BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,945,938	T→G	10.1%	L398R (CTT→CGT)	EO946_RS20305 →	Na+/H+ antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,945,938	0	T	G	10.1%	1025.2 / 17.7	493	L398R (CTT→CGT)	EO946_RS20305	Na+/H+ antiporter
Reads supporting (aligned to +/- strand): ref base T (220/190); new base G (72/3); total (300/193)
Fisher's exact test for biased strand distribution p-value = 2.98e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 519 total aligned reads displayed.

GGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTCAAACATAATTTACTTATTCACGAAGCTGT  >  NZ_CP034943/3945799‑3946076

ggcgggcTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTCCCATGTTGTTCAGGGGCTCACCAAAAAGCCGct                                                                                                                                            >  2:1002749/1‑140 (MQ=255)

ggcgggcTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCATTTTTTTAAGGGGCTAACCATAAAGCCGct                                                                                                                                            >  2:1688684/1‑140 (MQ=255)

    ggcTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGcttctt                                                                                                                                        >  2:263722/1‑140 (MQ=255)

    ggcTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGcttctt                                                                                                                                        <  1:4113015/140‑1 (MQ=255)

      cTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGa                                                                                                                                      <  1:1761689/140‑1 (MQ=255)

      cTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGCGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTAACTTGTTGTTAAGGTGCTAACCATCAAGCCGCATATTGa                                                                                                                                      >  2:2311768/1‑140 (MQ=255)

       tGAATGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGaa                                                                                                                                     <  1:4779908/140‑1 (MQ=255)

       tGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGaa                                                                                                                                     <  2:4712153/140‑1 (MQ=255)

       tGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGaa                                                                                                                                     <  2:2420398/140‑1 (MQ=255)

       tGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTGTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGaa                                                                                                                                     <  2:3976722/140‑1 (MQ=255)

               tctcTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAgg                                                                                                                             >  2:728822/1‑140 (MQ=255)

               tctcTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCTTTTTGTTCAGGGGGTCACCAACAAGCCGCGTGTTGAACGGGTAgg                                                                                                                             >  1:2302276/1‑140 (MQ=255)

                     tCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTGCCTTGTTGTTGAGGGGCTCACCATCAAGCCGCGTGTTGAACGGTTAGGTGTGaa                                                                                                                       >  1:792796/1‑140 (MQ=255)

                        aTTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTGCCTTGTTGTTCAGGGGGTCACCATCAAGCCCCTTGTTTAACGGTTAGGGGTGAATCa                                                                                                                    >  1:1146902/1‑140 (MQ=255)

                        aTTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCa                                                                                                                    >  2:2971381/1‑140 (MQ=255)

                          tGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCTCCTTCAGTGTCGTGTTGTTTTACCTTGTTGTTCAGGGGCTCACCAACAAGCCCCTGATTGAAAGGTTAGGTGTTAATCaaa                                                                                                                  >  1:3589168/1‑140 (MQ=255)

                          tGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTGCCTTGTTGGTGAGGGGCGCACCATCAAGCCGCTTCGTGAACGGTTGGGTGTGGATaaaa                                                                                                                  >  1:2170769/1‑140 (MQ=255)

                          tGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTGCCGTGTTTTTGAGGGGCTCCCCAACAAGCCGCTTCGTGAACGGGTTGGTGTGAATCaaa                                                                                                                  >  1:4528337/1‑140 (MQ=255)

                          tGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCTTGTTTTTCAGGGGCTCACCCAAAAGCCGCTTCTTGAACGGTTAGGTGTGAATaaaa                                                                                                                  >  1:6858382/1‑140 (MQ=255)

                          tGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTGTTGTTTTGCCTGGTTGGTCCGGGGCGCACCATCCATCCGCGTCTTGTACGGGTCGGTGGGAATaaaa                                                                                                                  >  1:2397289/1‑140 (MQ=255)

                          tGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTGTTGTTTTCCCTTGTTGTTCAGGGGCTAACCATCAAGACGCTTGGTGAACCGTTAGGTGTGAATCaaa                                                                                                                  >  1:4889439/1‑140 (MQ=255)

                              cTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAaaga                                                                                                              <  1:6099509/140‑1 (MQ=255)

                               tCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCGTGTTGTTCAGGGGCTCACCCTCAATCCGCTTATTTAAAGGTTAGGTGTGAATAAAAaacaa                                                                                                             >  2:2068297/1‑140 (MQ=255)

                               tCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTGTTGTTTTGCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAaagaa                                                                                                             >  1:3804964/1‑140 (MQ=255)

                                 gTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTCCCGTGTTGGTGAGGGGCTCACCCTCAAGCCGCGTCTTGAAAGTTTAGGGGTGGATCAAAaagaaga                                                                                                           >  2:308601/1‑140 (MQ=255)

                                 gTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGGGTCGTTTTGTTTTCCCTGGTTGTTCCGGGGCTCACCATACACCCGGTTCGTGTACGGTTAGGTGTGAATCAAAtagaaaa                                                                                                           >  1:7509576/1‑138 (MQ=255)

                                    cTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTGCCGTGTTGTTGAGGGGCTCACCATCAAGCCGCTTGTTGTACGGTTAGGTGTGAATCAAAaagaagaatg                                                                                                        >  1:7371994/1‑138 (MQ=255)

                                     tGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGc                                                                                                       <  2:4418583/140‑1 (MQ=255)

                                     tGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCGTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGc                                                                                                       <  2:6094985/140‑1 (MQ=255)

                                         cctgcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTCCCTTGTTGTTCAGGGGCTCAACAACAAGCCGCTTCTTTAAAGGTTAGGTGTGAATCAAAAAGAAGAAGGCAAcc                                                                                                   >  2:3757279/1‑140 (MQ=255)

                                           tgcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAg                                                                                                 <  1:1002749/140‑1 (MQ=255)

                                            gcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTGCCGTGTTGTTGGGGGGCTCACCATCAAGCCGCTGCGTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAgg                                                                                                >  1:2921206/1‑140 (MQ=255)

                                            gcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTAAAATGTTGTTGAAGGGGTCAACAACAAAACGCGAAATTAACCGGTAGGTGTGAATCAAAAATAAAAAAGAAACCact                                                                                                >  1:3222307/1‑138 (MQ=255)

                                            gcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTTTTTTTCAGGGGCTCACCATCAATAACCTTCTTTAAAGGTTAGGGTTTAATAAAAAAGAAGAAGGCAACCAgg                                                                                                >  2:3537158/1‑140 (MQ=255)

                                            gcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTTTTCAGGGGCTCACCATCAAGCCGCTTCTTTAACGGTTATGTGTGAATCAAAAAGAATAAGGCAACCAgg                                                                                                >  2:2782599/1‑140 (MQ=255)

                                            gcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAgg                                                                                                >  2:624280/1‑140 (MQ=255)

                                            gcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCGTGTTGTTCAGTGGCTCACAATAAAGCCGCTTCTTTAACGGAAAGGTGTGAAACAAAAAGAAGAAGGCAACCAgg                                                                                                >  2:878602/1‑140 (MQ=255)

                                            gcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCCAGTTGTTCAGGGGCTCACCATCAAGCCGCGTCTTGAACGGGTAGGTGTGAATCAAAAAGAACAAGGCAACCAgg                                                                                                >  2:7761247/1‑140 (MQ=255)

                                             cctAGAGACTTCCCGGGACGTGAAGACATTCTTGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTTTGAATCAAAAAGAAGAAGGCAACCAGGa                                                                                               <  1:1691843/140‑1 (MQ=255)

                                             cctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGa                                                                                               <  1:7278207/140‑1 (MQ=255)

                                             cctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGa                                                                                               <  1:2091273/140‑1 (MQ=255)

                                             cctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGGTCATGGGCTCACCATCAAGCCGCTTCTTGAAAGGTTATGTTTGAATAAAAAAGAAGAAGGCAACCAGGa                                                                                               >  2:3252149/1‑140 (MQ=255)

                                             cctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTGTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCGTGAACGGTTAGGTGTGAATCAAAAAGAATAAGGCAACCAGGa                                                                                               >  2:1469590/1‑140 (MQ=255)

                                                 gagaCTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTTCCTTTTTGTTTAGGGGGTCACCATAAAGCCGCTTCTTGTACGGTGTGGTGTGTATAAAAAATAAGAAGGCAAACAGGGGTAt                                                                                           >  1:709411/1‑140 (MQ=255)

                                                 gagaCTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCAACCAGCCGCTTCTTGAAAGGTTAGGTGTGAATCAAAAAGAAGAAGGGAACCAGGAGTAt                                                                                           >  1:5426036/1‑140 (MQ=255)

                                                   gaCTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCAACAAGCCGCGTCTTGAACGGTTAGGGGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGa                                                                                         >  1:3396776/1‑140 (MQ=255)

                                                     cTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCTTTTTGTTTTCCCGTTTTTTAAAGGGGCAAACCATAAATCCGCATATTTAAGCGTTTTGGGTTAATAAAAAAAAAGAAAGAAAACAGGAGTAAGAgg                                                                                       >  1:1443552/1‑140 (MQ=255)

                                                      ttCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTGCCTTGTTTTTCAGGGGCTCAACATCAAGCCGCTTCTTTAAACGTTATGTGTGAATCAAAAAGAACAATGCAACCACGAGGATTAGGa                                                                                      >  2:505362/1‑140 (MQ=255)

                                                      ttCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGGTAGGGGTGAATCAAAAAGAATAAGGCAACCAGGATTATGAGGa                                                                                      >  2:1911904/1‑140 (MQ=255)

                                                       tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTGCCTTGTTTTTCAGGGGGGCACCCTCAAGCCGCGTCTTGAACGGGTGGGGGTGGATCAAAAAGAAGAAGGCCACCAGGCGTATGAGGaa                                                                                     >  1:2696951/1‑140 (MQ=255)

                                                       tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTTCCTGGTTGTTGCGGGGCTCCCCATCAAGCCGCTTCGTGAACGGGTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGCGGATGAGGac                                                                                     >  1:5135144/1‑139 (MQ=255)

                                                       tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTTCCGTGTTTTTGAGGGGGTCACCAAAAAGCCCCGTGGTGCACGGGTAGGTGTGAATCAAAAAATATAAGGCCACCAGGGGTATTGGGaa                                                                                     >  1:671348/1‑140 (MQ=255)

                                                       tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTTTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATAAAAAATAAGAAGGCAACCAGGAGTATGAGGaa                                                                                     >  2:109449/1‑140 (MQ=255)

                                                       tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTGAGGGGCTCACCATCAAACCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGaa                                                                                     >  2:158596/1‑140 (MQ=255)

                                                       tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCTTGTTTTTAAGGGGCTCACCATCAACCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCACGAGTATGATGaa                                                                                     >  2:5147234/1‑140 (MQ=255)

                                                       tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCTTGTTGTTCAGGGGCTCACCATAAGACCGCTTCTTGTACGGGTAGGGGTGAATAAAAAAATAGAACGCAACCAGGAGTAGGAGGaa                                                                                     >  1:4247430/1‑140 (MQ=255)

                                                       tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCGTGTTGTTCAGGGGCGCACCAAAAAGCCGCGTCTTTAACCGTTATGTGTGTAACAAAAAAAACAAGGCAACCAGGAGTATGAGGaa                                                                                     >  1:1248068/1‑140 (MQ=255)

                                                        cccGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTAGCTTGTTTTTGAGGGGCTCACCCTCAAGCCCCTTCTTGTACGGGTTTGTGTGTATCAAAAAAAAAAAGGCAACCACGAGTATGTGGAAt                                                                                    >  1:5826234/1‑140 (MQ=255)

                                                            ggACGTGAAGACAGTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCGTGTTGGTCAGGGGCTCACCATCAAGCCGCTTCGTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAAttgct                                                                                <  2:4120596/140‑1 (MQ=255)

                                                                          tCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTGCCTTGTTGTTGAGGGGCTCACCATCAAGCCGCTTGTTGTACGGGTTGGGGTGAATCAAAAAGAATAAGGCAACCAGGAGTATGAGGAATTTGTTGCAAAAGGAAATc                                                                  >  1:7680696/1‑140 (MQ=255)

                                                                          tCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTGCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTTAATCAAAAAGAAGAAGGCAAACAGGAGTATTAGGAATTGCTTGCAAAAGGaaaac                                                                  >  2:548589/1‑136 (MQ=255)

                                                                          tCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTAAGGGGCTCAACATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATAAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTTCTTGCAAAATGACATc                                                                  >  2:6702330/1‑140 (MQ=255)

                                                                             ggTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAaagaagaag                                                                                                         >  2:1710598/1‑98 (MQ=255)

                                                                                  tCGCCTTCAGTGTCGTTTTGTTTTGCCTTGTTGGTGAGGGGCTCACCAACAAGCCGCGTGTTTTAGGGGTAGGTGTGATTGACAAAGTAGAAGGGAACCAGGTGGATGTGGCATTGGGTGCACAAGGACATCGCGTAgga                                                          >  1:5637682/1‑138 (MQ=255)

                                                                                  tCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTGAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAACAACGCAACCAGGAGTATGAGGAATTGCTTGCAAAATGACAAAGCTTAGaa                                                          >  2:119644/1‑140 (MQ=255)

                                                                                  tCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGaa                                                                                     <  1:6505687/113‑1 (MQ=255)

                                                                                  tCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGaa                                                          >  2:5389353/1‑140 (MQ=255)

                                                                                  tCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGGTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGaa                                                                                     >  2:6505687/1‑113 (MQ=255)

                                                                                               cGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAaag                                             <  1:3020472/140‑1 (MQ=255)

                                                                                               cGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAaag                                             <  2:7635539/140‑1 (MQ=255)

                                                                                                 ttttgttttgCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTATTTAACGGTTATGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATTAGGAATTGCTTTCAAAAGGACAAAGCTTATAAACCGCCATCAaagaa                                           >  2:4199986/1‑140 (MQ=255)

                                                                                                        ttCCGTTGTTGTTCAGGGTCTCACCATGCAGCCGCTTCTTGAATGTTTATGTGTGAATCAAAAAGTAGATGGCATACAGGAGTATTATGAATTGCTTGCAAAATTACATCGCTTAGAAACCGCCATCAAAGAAGTCCatc                                    <  2:4563694/140‑3 (MQ=255)

                                                                                                        ttCCCTTGTTGTTCAGTGGCTCACCATCAAGCCGCTTCTTGAACGGTTATGTGTTAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGATTGCAAAATGACATATATTATAAAAAGCCATCAAAGAAGTCCAGc                                    <  2:2361372/140‑1 (MQ=255)

                                                                                                        ttCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGc                                    <  2:4311860/140‑1 (MQ=255)

                                                                                                        gtCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGc                                    <  2:1970502/139‑1 (MQ=255)

                                                                                                          cccTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCaa                                  <  1:2061555/140‑1 (MQ=255)

                                                                                                           ccTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGc                                    >  2:2576917/1‑137 (MQ=255)

                                                                                                             ttgttgTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTc                               <  2:5826234/140‑1 (MQ=255)

                                                                                                               gttgttCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTCaa                             <  2:6984514/140‑1 (MQ=255)

                                                                                                                ttgttCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTCaaa                            <  2:7145826/140‑1 (MQ=255)

                                                                                                                ttgttCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTCaaa                            <  2:5940457/140‑1 (MQ=255)

                                                                                                                ttgttCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGCTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTAGGAGGAATTGCGTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTCaaa                            >  1:2535488/1‑140 (MQ=255)

                                                                                                                     cAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTCAAACATaa                       >  1:6376690/1‑140 (MQ=255)

                                                                                                                         ggCTCCCCCTCAAGCCGCGTCTTGAACGGTTAGGTGTGCATCAAAAAGAAGCAGGCAACCAGGAGTATGAGGAATCGCTCGCAACAGGCCATCGCTTAGAAACCGCCATCCAAGACGTCCCGCACGTCACACAGAATGTa                   <  2:5575004/140‑1 (MQ=255)

                                                                                                                           cTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTCAAACATAATTTACt                 <  2:5425042/140‑1 (MQ=255)

                                                                                                                            tCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTCAAACATAATTTACtt                <  2:2170769/140‑1 (MQ=255)

                                                                                                                             cACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAATAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTCAAACATAATTTAATTa               >  1:6321396/1‑140 (MQ=255)

                                                                                                                             cACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAACACGCCATCAAAGAAGTCCAGCAAGTCAAACATAATTTAATTa               >  2:2116354/1‑140 (MQ=255)

                                                                                                                             cACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGt                                >  1:5276472/1‑123 (MQ=255)

                                                                                                                             cACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGt                                >  1:4389706/1‑123 (MQ=255)

                                                                                                                             cACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTCAAACATAATTTACTTa               >  1:1611570/1‑140 (MQ=255)

                                                                                                                             cACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTCAAACATAATTTACTTa               >  1:7646299/1‑140 (MQ=255)

                                                                                                                             cACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTCAAACATAATTTACTTa               >  1:2031259/1‑140 (MQ=255)

                                                                                                                                 aTCAAGCCGCTTCTTGAACGGTTAGGTGTTAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTCAAACATAATTTACTTATTCa           <  2:7455762/140‑1 (MQ=255)

                                                                                                                                 aTCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTCAAACATAATTTACTTATTCa           <  2:3459397/140‑1 (MQ=255)

                                                                                                                                  tCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTCAAACATAATTTACTTATTCAc          >  2:530522/1‑140 (MQ=255)

                                                                                                                                      gCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTCAAACATAATTTACTTATTCACGAAg      <  2:6375620/140‑1 (MQ=255)

                                                                                                                                          cttcttGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTc                               >  1:695058/1‑111 (MQ=255)

                                                                                                                                          cttcttGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTCAAACATAATTTACTTATTCACGAAGCTGt  >  2:5248866/1‑140 (MQ=255)

                                                                                                                                          cttcttGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTCAAACATAATTTACTTATTCACGAAGCTGt  >  2:4901604/1‑140 (MQ=255)

GGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTCAAACATAATTTACTTATTCACGAAGCTGT  >  NZ_CP034943/3945799‑3946076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`