Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,976,149 G→T 6.2% G19G (GGC→GGA rlmH ← 23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,976,1490GT 6.2% 1579.7 / 21.9 554G19G (GGC→GGArlmH23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH
Reads supporting (aligned to +/- strand):  ref base G (155/343);  new base T (48/4);  total (206/348)
Fisher's exact test for biased strand distribution p-value = 2.08e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 554 total aligned reads displayed.

GGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAGATC  >  NZ_CP034943/3976011‑3976287
                                                                                                                                          |                                                                                                                                          
 gATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGcc                                                                                                                                          <  2:2993413/140‑1 (MQ=255)
 gATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGcc                                                                                                                                          <  1:2264335/140‑1 (MQ=255)
     ggATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCcttgc                                                                                                                                      <  2:2218964/140‑1 (MQ=255)
     ggAGGCGGTCGCCTTCTTTGTCTTGTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCcttgc                                                                                                                                      <  2:4467386/140‑1 (MQ=255)
           ggTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAgg                                                                                                                                >  2:7647391/1‑140 (MQ=255)
                ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTTCGTATGCCGAAAGTCGTTTCGTGTATTATTGAATTGCTTGCTTGATGTAttt                                                                                                                           >  1:7148732/1‑140 (MQ=255)
                ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTAttt                                                                                                                           >  2:1232019/1‑140 (MQ=255)
                ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTATTCAATGCCTTGCTTTAGGTAttt                                                                                                                           >  2:7013619/1‑140 (MQ=255)
                ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGGTTCGTGTATTCTTCAATGCCGTGCTTGAGGTAttt                                                                                                                           >  2:2845040/1‑140 (MQ=255)
                ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTATTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTAttt                                                                                                                           >  2:7515297/1‑140 (MQ=255)
                ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCGTAGGTTTTGCGGCGCTTTTTAGTCCGGGCGTTTGATGATTTGGCTTTTTTGGTTTGGCGAAAGTAGTTTCGTGTATTCTTGCATTCCGTGCGTGTGGTAttt                                                                                                                           >  1:2575916/1‑140 (MQ=255)
                ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCGTAGGTTTTCCGGCGCTTTTTGGTGCGGGAGTTCGATGATGTCGATTTTTGCGTTGGCCGAAAGTCGTTTCGTGTTTTGTTGAATGCCTTGCTTGTGGTAttt                                                                                                                           >  1:6211696/1‑140 (MQ=255)
                      ttGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCttt                                                                                                                     <  1:2435555/140‑1 (MQ=255)
                       tGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCGTGCTTTAGGTATTTTTCtttt                                                                                                                    >  1:7717602/1‑140 (MQ=255)
                       tGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTAGTCCGGGAGTTCGATGATGTAGATTTTTGCGTAGGCCGAAAGTGGTTTAGTGTATTCTTCAATTCCTTGCTTGAGTTATTTTTCtttt                                                                                                                    >  2:7709953/1‑140 (MQ=255)
                       tGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTCGTCCGGGATTTAGATGATGTCGATTTTTTCGTATGCCGAAAGTCGGTTAGTGTATTATTAAAATCCGTTCTTGAGGtatttttatttt                                                                                                                    >  1:1571879/1‑140 (MQ=255)
                           ttttATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTATTCCGGGAGTTCGATGATGTCGATTATTGCGTAGGCCGAAAGTCGTTTCGTGTAATCTTAAAAGGCTTTATTTAGGTATTTTtattttattt                                                                                                                >  2:1078278/1‑140 (MQ=255)
                           ttttATGATTTTCATGTCCTGGTCGCTTAGGGTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTATTTTAGtt                                                                                                                >  2:5047171/1‑140 (MQ=255)
                              tATGATTTTCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTCGTCCGGGAGTTCGATGATTTCGAATTTTGCGTAGGCCGAAAGTCGTTTCGTTTATTCTTCAATTCCATTCTTGATGTATTTTTAATTTAGTTTTc                                                                                                             >  2:250722/1‑140 (MQ=255)
                                  aTTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGAt                                                                                                         <  2:6271644/140‑1 (MQ=255)
                                  aTTTTCAGGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGAGGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGAt                                                                                                         <  1:1072505/140‑1 (MQ=255)
                                   ttttCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGCGTTCGATGATGTGGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTATTTTAGTTTTCCGAtt                                                                                                        >  2:5390785/1‑140 (MQ=255)
                                   ttttCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGAtt                                                                                                        <  1:1003779/140‑1 (MQ=255)
                                       cATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTca                                                                                                    <  1:3987923/140‑1 (MQ=255)
                                       cATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTca                                                                                                    <  1:6709076/140‑1 (MQ=255)
                                       cATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGGCGATTTTTGCGTAGGCCGAAATTCCTTTGGTGTATTCTTCAATTCCTTGGTTGAGGTATTTTTGTTTTTTTTTTACGCTTGTca                                                                                                    >  1:3032051/1‑140 (MQ=255)
                                       cATGTCCTGGTCGCTTAGGTTGTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTca                                                                                                    <  1:13765/140‑1 (MQ=255)
                                          gTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACaa                                                                                                 <  2:1653276/140‑1 (MQ=255)
                                          gTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACaa                                                                                                 <  1:2860471/140‑1 (MQ=255)
                                            ccTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAata                                                                                               <  2:5635237/140‑1 (MQ=255)
                                              tGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAATTCGTTTCGTGTATTATTAAATTCCTTGCTTGTTGTAATTTTGTTTTTTTTTTCCGCTTGTCACAATAtt                                                                                             >  1:1921120/1‑140 (MQ=255)
                                               ggTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                                                            <  2:3290493/140‑1 (MQ=255)
                                               ggTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                                                            <  2:5946682/140‑1 (MQ=255)
                                               ggTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                                                            <  2:6643701/140‑1 (MQ=255)
                                               ggTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                                                            <  2:6868296/140‑1 (MQ=255)
                                               ggTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                                                            <  2:861445/140‑1 (MQ=255)
                                               ggTCGCTTAGGTTGTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                                                            <  2:3788005/140‑1 (MQ=255)
                                               ggTCGATTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                                                            <  2:4148012/140‑1 (MQ=255)
                                                gTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGa                                                                                           <  2:1892909/140‑1 (MQ=255)
                                                gTCGCTTAGGTTTTCCGGCGCTTTTTAGTCCGGGAGTTAGATGAATTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTATTCAATTCCTTGCTTTAGGTATTTTTGTTTTATTTTTTCGCATGTCAGAAAAttta                                                                                           >  1:4309380/1‑138 (MQ=255)
                                                 tCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGat                                                                                          <  2:7121841/140‑1 (MQ=255)
                                                 tCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGat                                                                                          >  2:5448702/1‑140 (MQ=255)
                                                 tCGCTTAGGTTTTCCGGCGCTTTGTCGTCCGTGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGat                                                                                          <  2:2704222/140‑1 (MQ=255)
                                                 tCGCTTAGGTTTTCCCGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATCCCGTGCTTGAGGTATTTTTATTTTAGTTTTGCTATTGTCACAAAATTGat                                                                                          >  2:744248/1‑140 (MQ=255)
                                                  cGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGata                                                                                         <  2:5193868/140‑1 (MQ=255)
                                                      tatgTTTTCCGGCGATTTTTCGTCAGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAATTCGTTTCGTGTATTCTTCAATTCCTTGATTGTGGTTTTTTTCTTTTAGTTTTCGGATTGTCACAATATTGATATTca                                                                                     <  1:1237459/137‑1 (MQ=255)
                                                      tAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTca                                                                                     <  1:2081637/140‑1 (MQ=255)
                                                      tAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTca                                                                                     <  1:7340209/140‑1 (MQ=255)
                                                      tAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTca                                                                                     <  1:7654778/140‑1 (MQ=255)
                                                          ttttCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAgg                                                                                 <  1:4570288/140‑1 (MQ=255)
                                                           tttCCGGCGCTTTGTCGTCCGGGAGTTCGATGAGGTCGACACTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCCATGCCTTGCTTGAGGTATTTTTCTTTTAGTGTTCCGATGGTCACACTATTGATATTCACAGGt                                                                                <  1:5636005/140‑1 (MQ=255)
                                                             tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCa                                                                              >  2:2193214/1‑140 (MQ=255)
                                                             tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTAACAGGTCa                                                                              >  1:6921822/1‑140 (MQ=255)
                                                             tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTGGCTTGAGGTATTTTTGTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCa                                                                              >  1:3657014/1‑140 (MQ=255)
                                                                  cgcTTTGTCGTCCGGGAGTTCGAGGATGTCGATATTTGCGTAGGCCGAAAGTCGTTTCGTGGATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATcccc                                                                         <  1:6037739/140‑1 (MQ=255)
                                                                   gcTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                                                            <  1:5788294/120‑1 (MQ=255)
                                                                   gcTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                                                            >  2:2550848/1‑120 (MQ=255)
                                                                   gcTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCa                                                                        <  1:3499967/140‑1 (MQ=255)
                                                                   gcTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCa                                                                        <  1:3134566/140‑1 (MQ=255)
                                                                   gcTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCa                                                                        <  1:7238826/140‑1 (MQ=255)
                                                                                ggAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAg                                                           <  1:487914/140‑1 (MQ=255)
                                                                                ggAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAg                                                           <  1:167014/140‑1 (MQ=255)
                                                                                 gAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGt                                                          <  1:5303281/140‑1 (MQ=255)
                                                                                  aGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGtt                                                         <  1:5213964/140‑1 (MQ=255)
                                                                                   gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCACTGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  2:4844560/140‑1 (MQ=255)
                                                                                   gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCACTGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  1:5100038/140‑1 (MQ=255)
                                                                                   gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  2:146642/140‑1 (MQ=255)
                                                                                   gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  1:3012346/140‑1 (MQ=255)
                                                                                   gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  1:95700/140‑1 (MQ=255)
                                                                                   gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  1:828019/140‑1 (MQ=255)
                                                                                   gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  1:1475722/140‑1 (MQ=255)
                                                                                   gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  1:7573394/140‑1 (MQ=255)
                                                                                   gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCGTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  1:4016658/140‑1 (MQ=255)
                                                                                    ttCGATGAGGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATACTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATACCCCCTTTAAAAACAAGTTAt                                                       >  2:2005050/1‑140 (MQ=255)
                                                                                     tCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAtt                                                      <  1:1841561/140‑1 (MQ=255)
                                                                                     tCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAtt                                                      <  2:3478239/140‑1 (MQ=255)
                                                                                           atgTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCCCAATATTGATATTCACAGGTCATCCCCACGTTAAAAACACGTTATTCatata                                                >  1:6821525/1‑140 (MQ=255)
                                                                                             gtagATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTGTCAGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg                                              <  2:2328495/137‑1 (MQ=255)
                                                                                             gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg                                              <  1:5405840/140‑1 (MQ=255)
                                                                                             gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg                                              <  2:4976865/140‑1 (MQ=255)
                                                                                             gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg                                              <  1:4780231/140‑1 (MQ=255)
                                                                                                 aTTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtgttgt                                          >  2:610077/1‑140 (MQ=255)
                                                                                                  tttttGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTc                                         <  2:4638185/140‑1 (MQ=255)
                                                                                                    tttGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCca                                       <  1:7463779/140‑1 (MQ=255)
                                                                                                    tttGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCca                                       <  1:5472453/140‑1 (MQ=255)
                                                                                                    tttGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCca                                       <  1:3818628/140‑1 (MQ=255)
                                                                                                      tGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCcaca                                     >  1:4178364/1‑140 (MQ=255)
                                                                                                       gCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACaa                                    <  1:6434779/140‑1 (MQ=255)
                                                                                                       gCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACaa                                    <  2:3082467/140‑1 (MQ=255)
                                                                                                            ggCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATtgtg                               <  2:4758494/140‑1 (MQ=255)
                                                                                                             gCCGAAAGTCGTTTCGTGTATTCTTCAATGCCGTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCAACATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATCTGTTGTCCACAATTGTgg                              >  1:657244/1‑140 (MQ=255)
                                                                                                                 aaaGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGatat                          <  1:409751/140‑1 (MQ=255)
                                                                                                                       gTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATCTTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATTTTGTCAACAATTGAGGATATCTATTc                    >  1:7021568/1‑140 (MQ=255)
                                                                                                                       gTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCat                                                   <  2:7260402/109‑1 (MQ=255)
                                                                                                                       gTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCAATTGTCATAATATTGATATTCACAGGTCATCCCCACGTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTc                    >  1:3327334/1‑140 (MQ=255)
                                                                                                                        tttCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAACTTATTCATATATGTTGTCCACAATTGTGGATATCttttat                   >  1:5973557/1‑138 (MQ=255)
                                                                                                                             tgtATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg                                              >  2:3949613/1‑108 (MQ=255)
                                                                                                                             tgtATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt              <  1:463847/140‑1 (MQ=255)
                                                                                                                             tgtATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTGTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt              <  2:6436164/140‑1 (MQ=255)
                                                                                                                                         tGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAGATc  >  1:7516523/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
GGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAGATC  >  NZ_CP034943/3976011‑3976287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: