BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	240,207	G→T	10.3%	Q109K (CAA→AAA)	EO946_RS01360 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	240,207	0	G	T	10.3%	1472.0 / 38.1	687	Q109K (CAA→AAA)	EO946_RS01360	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (257/331); new base T (97/2); total (354/333)
Fisher's exact test for biased strand distribution p-value = 1.15e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 689 total aligned reads displayed.

GTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTA  >  NZ_CP034943/240068‑240345

  gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGaa                                                                                                                                          <  2:2901672/140‑1 (MQ=255)

         agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCttt                                                                                                                                   >  2:2144288/1‑140 (MQ=255)

          gagagaAGGAGCATCTCTGATGTTGTAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAATTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGATTTGGTCAATTTTAATGGCgttt                                                                                                                                  >  1:5635349/1‑140 (MQ=255)

          gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCtttt                                                                                                                                  >  2:1069994/1‑140 (MQ=255)

          gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTGAAGTTTTTGGAGATTTGAATGGCtttt                                                                                                                                  >  1:5762696/1‑140 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTGTTTGTATTTTTTGGAGATTTGAATTGCtttta                                                                                                                                 >  1:757734/1‑139 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCTGATTTTTTTTTTAAGTTTTGGGAGATTTTAATTTCtttta                                                                                                                                 >  1:2058984/1‑139 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAATTTTTGTGAGATTTTAATTGGtttta                                                                                                                                 >  1:4137202/1‑139 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGTCTTTTc                                                                                                                                 >  2:4760316/1‑140 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAACTTTTTGGAGATTTGAATTGGTTTTc                                                                                                                                 >  1:1009165/1‑140 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTTATTTTTTGGAGATTTTAATTGCtttta                                                                                                                                 >  1:4908039/1‑139 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAATTTTTTGTAGATTTTAATGTCtttta                                                                                                                                 >  1:6250872/1‑139 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAATTTTTGTGAGATTTGAATGGCttttg                                                                                                                                 >  1:6129379/1‑139 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTTGAGATTTTTATGTGTTTTc                                                                                                                                 >  1:5861572/1‑140 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTGGAGATTTGAATTGCtttta                                                                                                                                 >  1:7144195/1‑139 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTGGAGAGTTGAATGGCTGTTc                                                                                                                                 >  1:1349469/1‑140 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGGAGTGTGGGGATGTTTGAATGGCTTTTc                                                                                                                                 >  2:4172386/1‑140 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTTAATGGCTTTTc                                                                                                                                 >  1:5466176/1‑140 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                                 >  2:2289379/1‑140 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTGAAGTTTTGGGAGATTTTAATTGCTTTTc                                                                                                                                 >  2:4615260/1‑140 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTGGGATTTTTGTTTTTAGTTTTTGGTGCTTTTAATTGCGTTTc                                                                                                                                 >  1:3958873/1‑140 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTAGGATTTTTTTTTTAATTTTTGGGAGATTTGAATTGCTTTTc                                                                                                                                 >  1:1282942/1‑140 (MQ=255)

           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTAGGAATTTTTTTTTAATTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                                 >  1:3565468/1‑140 (MQ=255)

             agaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTCCg                                                                                                                               >  2:4298230/1‑140 (MQ=255)

               aaGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTGAATTTTTGGGAGAATTTAATTGCTTTTCCGtt                                                                                                                             >  2:2464572/1‑140 (MQ=255)

                  gAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGTGATTTTAATGGCTTTTACGttttt                                                                                                                          >  1:1659747/1‑140 (MQ=255)

                        ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGACGTCGATCCTTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                                                    <  1:1516642/140‑1 (MQ=255)

                        ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                                                    <  1:985871/140‑1 (MQ=255)

                             aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAATTTTTTGGAGATTTGAATGGCTTTTACGTTTTTAaaaaaaaatg                                                                                                               >  1:85440/1‑136 (MQ=255)

                             aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTGTTAAATCAAGAgg                                                                                                               >  2:6136412/1‑140 (MQ=255)

                                  ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGTTCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGGTTTTGCGTTTTTAAAAAAAGAGGCAAGc                                                                                                          >  1:3650768/1‑140 (MQ=255)

                                  ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGAGATTTGAATGGCTTTTACTTTTTTAAAAAAAGAGGCAAGc                                                                                                          >  2:4519192/1‑140 (MQ=255)

                                  ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTTGGCGATTTGAATGGCTTTTCCGTTTTTATAAAAAGATGCAAGc                                                                                                          >  1:6989187/1‑140 (MQ=255)

                                  ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAGTCCAGc                                                                                                          >  2:7097233/1‑140 (MQ=255)

                                  ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                                          >  2:135544/1‑140 (MQ=255)

                                  ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGTATTTTTGGGAGATTTTAATTGCTTTTTCGTTTTTTAAAAAATAGGCAAGc                                                                                                          >  1:3442052/1‑140 (MQ=255)

                                        attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAAAGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                                    >  2:5092572/1‑140 (MQ=255)

                                         ttCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGTTTTGAATTGCTTTTACGTTTTTAAAAAAAAAGGCAAGCCAGATaa                                                                                                   >  1:3771282/1‑140 (MQ=255)

                                                  gCTGTTTTTGTATTTTTGTTCTAATCCGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTGTGAAGTTTTGGGAGATTGGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaa                                                                                          <  2:4912446/140‑1 (MQ=255)

                                                   cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGATGCAAGCCAGATAAAACCGacaaa                                                                                         >  2:5831470/1‑139 (MQ=255)

                                                   cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAAACCGacaaa                                                                                         >  2:2012301/1‑139 (MQ=255)

                                                   cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAATCCAGATAAACCCGacaaa                                                                                         >  2:7014208/1‑139 (MQ=255)

                                                   cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGTCTTTTCCGTTTTTAAAAAAACAGGCAAGCCAGATAAACCCGacaac                                                                                         >  2:6903309/1‑140 (MQ=255)

                                                   cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATTTCTTTTCCGTTTTTAAAACAAGATGCAAGCCAGATAAACCCGacaac                                                                                         >  2:7758956/1‑140 (MQ=255)

                                                   cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACAATACGGGCGTTTGCGGATTTTTGTTTGAAATTTTGTTAGAGTTTAAATGATATTCCGTATTTAATACAATATGCAACCCAGAGAAACACGaaaac                                                                                         >  2:3012426/1‑140 (MQ=255)

                                                        tttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAATTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAATAGGCAAGCCAGATAAACCCCAcaacaaaaa                                                                                    >  1:7717678/1‑140 (MQ=255)

                                                        tttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTTAAATTTTTGGAGATTTTAATGGCTTTTACGTTTTTAAAAAAAGAGGCAAACCAGATAAAACCGAcaacaacaa                                                                                    >  1:6811264/1‑140 (MQ=255)

                                                        tttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTAGGATTTTTTTTTTTATTTTTTGGAGATTTTAATTGCTTTTCCGTTTTTTAAAAAAAATGCAAGCCACCTAAACCCCAcaaaaaaaa                                                                                    >  1:2203831/1‑140 (MQ=255)

                                                             aTTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAaat                                                                               <  1:7480634/140‑1 (MQ=255)

                                                                tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAATTTTTGGGAGATTTGAATGGGGTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATTAACCCGTCAACAACAAAAaataat                                                                            >  2:5436756/1‑140 (MQ=255)

                                                                tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAaataat                                                                            <  2:5569947/140‑1 (MQ=255)

                                                                  tGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGCTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCt                                                                          <  1:1797371/140‑1 (MQ=255)

                                                                    ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                        <  1:113777/140‑1 (MQ=255)

                                                                    ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                        <  1:1445903/140‑1 (MQ=255)

                                                                    ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                        <  1:193388/140‑1 (MQ=255)

                                                                         aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                                                   <  1:5252738/140‑1 (MQ=255)

                                                                         aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                                                   <  1:2944175/140‑1 (MQ=255)

                                                                         aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                                                   <  1:7649069/140‑1 (MQ=255)

                                                                          tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                  <  2:7568023/140‑1 (MQ=255)

                                                                          tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                  <  2:2572945/140‑1 (MQ=255)

                                                                          tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                  <  1:2464572/140‑1 (MQ=255)

                                                                           aCGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTaa                                                                 <  1:460719/140‑1 (MQ=255)

                                                                              aaGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACt                                                              >  2:7647461/1‑140 (MQ=255)

                                                                                gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                                                            <  2:1692349/140‑1 (MQ=255)

                                                                                gTCGATCATACCGACTATACGGGCGTTTTCGGATTTTTGTATGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCACGTAAACTcc                                                            <  1:2424434/140‑1 (MQ=255)

                                                                                    aTCATTCCGACTATACGGGCGTTTTCGGATTTTTGTGTGAAGTTTTTGGAGATTTGAATGGCGTTTCCGGTTGTAAAACAAGAGGCAAGCCAGATAAAGCCCTCCAGAACTAAAAATAAACTAGCAAGTAAACTCcctgc                                                        >  1:5367331/1‑140 (MQ=255)

                                                                                     tCATTCCGACTATACGGGCGTTTTCTGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  2:346687/140‑1 (MQ=255)

                                                                                     tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  1:3176777/140‑1 (MQ=255)

                                                                                     tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  1:4473188/140‑1 (MQ=255)

                                                                                     tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  1:2824795/140‑1 (MQ=255)

                                                                                     tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  2:6278824/140‑1 (MQ=255)

                                                                                     tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  2:3689020/140‑1 (MQ=255)

                                                                                     tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  2:1483346/140‑1 (MQ=255)

                                                                                     tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  1:6617152/140‑1 (MQ=255)

                                                                                           cGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAAACCGACAACAACAAAAAATAATATAGCAAGTAAACTACATGCATGGGGt                                                 >  2:670175/1‑140 (MQ=255)

                                                                                             aCTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGCGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTACATGCATGGGGTaa                                               >  2:6552399/1‑140 (MQ=255)

                                                                                             aCTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTACGTTTTTAAAAAAAGAGGCAAGCCAGAAAAACACGACAACAACAAAAAATAATCTAGCAAATAAACTACATGCATGGGGTaa                                               >  2:2731337/1‑140 (MQ=255)

                                                                                             aCTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGGTTTTCCGTTTTTAAAAAAAGAGGCAAGCCAGATAAACCCGACACCAACAAAAAATAATATAGCAAGTAAAAACCATGCATGGGGTaa                                               >  2:3300698/1‑140 (MQ=255)

                                                                                                      gCGTTTTCGGATTTTTGTTTGAATTTTTTGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCCACAAAAACAAAAAATAATATAGCAAGTAAACTCCATGCATTGGGTAAATAATTAGt                                      >  2:900285/1‑140 (MQ=255)

                                                                                                      gCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTACATGCATGGGGTAATTCATTCGt                                      >  2:7179781/1‑140 (MQ=255)

                                                                                                       cGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTTAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGtt                                     >  2:5940255/1‑140 (MQ=255)

                                                                                                           ttaggATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCCACAACAACAAAAAAAAAACTAGCAAGTAAACTCCATGCATGGGGTAATTAATTCGTTGCaa                                 >  2:1214055/4‑140 (MQ=255)

                                                                                                           ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                 <  1:3319994/140‑1 (MQ=255)

                                                                                                           ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                 <  2:6012247/140‑1 (MQ=255)

                                                                                                           ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                 <  1:2289379/140‑1 (MQ=255)

                                                                                                           ttCGGATTTTTGTTGGCAGTTTTGGGAGAATTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                 <  1:4726251/140‑1 (MQ=255)

                                                                                                             ctgATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaaaa                               <  2:5209692/138‑1 (MQ=255)

                                                                                                                aTTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAatat                            <  1:913179/140‑1 (MQ=255)

                                                                                                                    ttgtatgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATaaaa                        <  2:1862868/140‑1 (MQ=255)

                                                                                                                      gtttgAAGTGTTGGGAGATCTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAAt                      <  1:4296915/140‑1 (MQ=255)

                                                                                                                       tttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATa                     <  2:6811264/140‑1 (MQ=255)

                                                                                                                        ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                    <  1:6402328/140‑1 (MQ=255)

                                                                                                                           aaGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGt                 <  1:6040466/140‑1 (MQ=255)

                                                                                                                                ttGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTa            <  2:4005074/140‑1 (MQ=255)

                                                                                                                                ttGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTa            <  1:5002809/140‑1 (MQ=255)

                                                                                                                                ttGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTa            <  1:2731337/140‑1 (MQ=255)

                                                                                                                                  gggAGCTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAAt          <  2:3222147/140‑1 (MQ=255)

                                                                                                                                  gggAGATTAGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAAt          <  1:7252140/140‑1 (MQ=255)

                                                                                                                                       aTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGcc     <  2:3113724/140‑1 (MQ=255)

                                                                                                                                        tttGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  2:7401834/89‑1 (MQ=255)

GTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTA  >  NZ_CP034943/240068‑240345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`