BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	240,211	G→T	16.3%	A107A (GCC→GCA)	EO946_RS01360 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	240,211	0	G	T	16.3%	1307.1 / 79.5	670	A107A (GCC→GCA)	EO946_RS01360	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (206/331); new base T (130/3); total (336/334)
Fisher's exact test for biased strand distribution p-value = 2.39e-41
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 675 total aligned reads displayed.

AAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGAT  >  NZ_CP034943/240072‑240348

aaGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTGAAGTTTTTGGAGATTTGTATg                                                                                                                                           >  2:3284304/1‑140 (MQ=255)

     agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGATTTGAATGGCttt                                                                                                                                      >  2:2831993/1‑140 (MQ=255)

     agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCttt                                                                                                                                      >  2:1045448/1‑140 (MQ=255)

      gagagaAGGAGCATCTCTGATGTTGTAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAATTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGATTTGGTCAATTTTAATGGCgttt                                                                                                                                     >  1:5635349/1‑140 (MQ=255)

      gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGGtttt                                                                                                                                     >  1:96996/1‑140 (MQ=255)

      gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTGAAGTTTTTGGAGATTTGAATGGCtttt                                                                                                                                     >  1:5762696/1‑140 (MQ=255)

       agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAATTTTTGTGAGATTTTAATTGGtttta                                                                                                                                    >  1:4137202/1‑139 (MQ=255)

       agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTTGGAGATTGTAATTGCTTTTc                                                                                                                                    >  2:5981618/1‑140 (MQ=255)

       agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAACTTTTTGGAGATTTGAATTGGTTTTc                                                                                                                                    >  1:1009165/1‑140 (MQ=255)

       agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAATTTTTTGTAGATTTTAATGTCtttta                                                                                                                                    >  1:6250872/1‑139 (MQ=255)

       agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAATTTTTGTGAGATTTGAATGGCttttg                                                                                                                                    >  1:6129379/1‑139 (MQ=255)

       agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTTGAGATTTTTATGTGTTTTc                                                                                                                                    >  1:5861572/1‑140 (MQ=255)

       agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTGGAGAGTTGAATGGCTGTTc                                                                                                                                    >  1:1349469/1‑140 (MQ=255)

       agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGGAGTGTGGGGATGTTTGAATGGCTTTTc                                                                                                                                    >  2:4172386/1‑140 (MQ=255)

       agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTc                                                                                                                                    >  2:1935837/1‑140 (MQ=255)

       agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATTGCTTTTc                                                                                                                                    >  2:1200664/1‑140 (MQ=255)

       agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                                    >  2:5068842/1‑140 (MQ=255)

       agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGAATTTTGTTTTAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                                    >  2:5322955/1‑140 (MQ=255)

       agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTGAAGTTTTGGGAGATTTTAATTGCTTTTc                                                                                                                                    >  2:4615260/1‑140 (MQ=255)

       agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTGGGATTTTTGTTTTTAGTTTTTGGTGCTTTTAATTGCGTTTc                                                                                                                                    >  1:3958873/1‑140 (MQ=255)

       agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTAGGATTTTTTTTTTAATTTTTGGGAGATTTGAATTGCTTTTc                                                                                                                                    >  1:1282942/1‑140 (MQ=255)

         agaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCg                                                                                                                                  >  2:127009/1‑140 (MQ=255)

         agaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTCCg                                                                                                                                  >  2:4298230/1‑140 (MQ=255)

             ggAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGTAGTTTTTGGAGATTTGAATGGCTTTTCCGtttt                                                                                                                              >  1:839975/1‑140 (MQ=255)

              gAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGTGATTTTAATGGCTTTTACGttttt                                                                                                                             >  1:1659747/1‑140 (MQ=255)

                 cATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTTAATTGCTTTTCCGTTTTTaaa                                                                                                                          >  2:213253/1‑140 (MQ=255)

                 cATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTACGTTTTTaaa                                                                                                                          >  2:6127656/1‑140 (MQ=255)

                   tctcTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAc                                                                                                                        >  2:1555450/1‑140 (MQ=255)

                    ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                                                       <  1:1515958/140‑1 (MQ=255)

                    ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                                                       <  2:626791/140‑1 (MQ=255)

                        gATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAagag                                                                                                                   <  1:2815307/140‑1 (MQ=255)

                         aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAATAgg                                                                                                                  >  2:3392040/1‑140 (MQ=255)

                             tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                              <  1:4259033/140‑1 (MQ=255)

                              ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGTTCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGGTTTTGCGTTTTTAAAAAAAGAGGCAAGc                                                                                                             >  1:3650768/1‑140 (MQ=255)

                              ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGTGATTTTTTTTTTAAGTTTTTTGAGATTTGAATTGCTGTTGAGTTTTTATAACAAGAGGCaaac                                                                                                             >  1:3137184/1‑138 (MQ=255)

                              ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGAGATTTGAATGGCTTTTACTTTTTTAAAAAAAGAGGCAAGc                                                                                                             >  2:4519192/1‑140 (MQ=255)

                              ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTTGGAGATTTGAATGTCTTTTACGTTTTTAAAAAAAGAGGCaaac                                                                                                             >  2:7385064/1‑138 (MQ=255)

                              ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAATAGGCAAGc                                                                                                             >  1:7449755/1‑140 (MQ=255)

                              ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGTATTTTTGGGAGATTTTAATTGCTTTTTCGTTTTTTAAAAAATAGGCAAGc                                                                                                             >  1:3442052/1‑140 (MQ=255)

                              ggAATTATTCAGAGATGCTGGTTTTGTATTTTTGTTCTAATACGAAGTCGCTCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGGGATTTGTATTGCTTTTCCGTTTTTAAAACAAGCGGCaacc                                                                                                             >  2:7095525/1‑138 (MQ=255)

                                    attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                                       <  1:5202905/140‑1 (MQ=255)

                                     ttCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGTTTTGAATTGCTTTTACGTTTTTAAAAAAAAAGGCAAGCCAGATaa                                                                                                      >  1:3771282/1‑140 (MQ=255)

                                          agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGTGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAAcccc                                                                                                 >  2:2692727/1‑139 (MQ=255)

                                          agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAATTTTTTGGAGATTTTAATTGGTTTTCCGTTTTTAAAAAAATAGGCAATCCAGATAAAACCg                                                                                                 >  1:500171/1‑140 (MQ=255)

                                               cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGATGCAAGCCAGATAAAACCGacaaa                                                                                            >  2:5831470/1‑139 (MQ=255)

                                               cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGATATTTGAATGTCTTTTACGTTTTTAAAAAAAAAGGCAAGCCAGATAAACCCGacaaa                                                                                            >  2:4752169/1‑139 (MQ=255)

                                               cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACAATACGGGCGTTTGCGGATTTTTGTTTGAAATTTTGTTAGAGTTTAAATGATATTCCGTATTTAATACAATATGCAACCCAGAGAAACACGaaaac                                                                                            >  2:3012426/1‑140 (MQ=255)

                                                    tttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGAcaacaaaaa                                                                                       >  2:6691566/1‑140 (MQ=255)

                                                    tttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTGGGATTTTTTTTTTAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAAAGGCAAGCCAGATAAACCCGAcaccaaaaa                                                                                       >  1:5331441/1‑140 (MQ=255)

                                                    tttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTAGGATTTTTTTTTTTATTTTTTGGAGATTTTAATTGCTTTTCCGTTTTTTAAAAAAAATGCAAGCCACCTAAACCCCAcaaaaaaaa                                                                                       >  1:2203831/1‑140 (MQ=255)

                                                    tttGTATTTTTGTTCTAATAATAATTATATAAAACCGACTATACGGTCGTTTTATTATTTTTTTTTTAAATTTTTTGAGATTTTAAAGGCTTTTCAGTTTTTAAATAAATATGCAAAACACATAAACACGaaaaaaaaaa                                                                                       >  2:6489861/1‑140 (MQ=255)

                                                            tttGTTCTAATACGAAGTCGATCATTCCGAGTATACGGGCGTTTTTGGATTTTTTTTTTAAATTTTTTGAGATTTTTATTTGTTTTCAGTTTTTAAAAAAACATCCAATCCACAGAAACACCACAACaaaaaaaaaaaaa                                                                               >  1:6552229/1‑139 (MQ=255)

                                                            tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTGTTTTTAGTTTTTGGGGATTTGAATTGCTTTTACGTTTTTAAAAAAAGAGGCAAGCCCGAAAAAACCCACAAAAACAAAAaataaa                                                                               >  1:4464468/1‑139 (MQ=255)

                                                            tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAaataat                                                                               <  2:5569947/140‑1 (MQ=255)

                                                            tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAaataat                                                                               >  2:7613325/1‑140 (MQ=255)

                                                              tGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGCTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCt                                                                             <  1:1797371/140‑1 (MQ=255)

                                                                ttCTAATCCGAAGTCGATCATTCCGACTATCCGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                           <  1:6784863/140‑1 (MQ=255)

                                                                ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                           <  1:113777/140‑1 (MQ=255)

                                                                ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                           <  1:193388/140‑1 (MQ=255)

                                                                ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                           <  1:1445903/140‑1 (MQ=255)

                                                                ttCTAATACGAAGTCGATCATTCCGAATATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                           <  1:7574732/140‑1 (MQ=255)

                                                                     aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGGTTTTGCGTTTTTAAAAAAAGATGCAAGCCAGATAAACCCGACAACAACAAAAAATAAAATAGCAAGt                                                                      >  2:697151/1‑140 (MQ=255)

                                                                     aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                                                      <  1:2944138/140‑1 (MQ=255)

                                                                     aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                                                      <  1:6961050/140‑1 (MQ=255)

                                                                     aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                                                      <  1:7648575/140‑1 (MQ=255)

                                                                     aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTTAAATTTTGGTAGATTTTAATTGCTTTTGGGTTTTTAAAACAATAGGGAAGCCAGGTAAACCCGAAAAAAAAAAAAAAAAATATAGGAAGt                                                                      >  1:2057062/1‑140 (MQ=255)

                                                                      taagAAGTCGATCATTCCGACTATTCGGGCGTTTTCTGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                     <  2:7741200/137‑1 (MQ=255)

                                                                      tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                     <  2:7155073/140‑1 (MQ=255)

                                                                           aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                                                <  1:3572099/140‑1 (MQ=255)

                                                                           aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                                                <  1:3304581/140‑1 (MQ=255)

                                                                            ttCGATCATTCCTACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGAGATTTGAATGGATTTTCCTTTTTTAAAAAAAGAGTCAAGCCAGATAAACCCGACAACATCATTAAATAATCTAGCAAGTAAACTcc                                                               <  2:1765691/139‑1 (MQ=255)

                                                                            gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                                                               <  1:5434414/140‑1 (MQ=255)

                                                                            gTCGATCATACCGACTATACGGGCGTTTTCGGATTTTTGTATGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCACGTAAACTcc                                                               <  1:2424434/140‑1 (MQ=255)

                                                                             tCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCca                                                              <  1:6403972/140‑1 (MQ=255)

                                                                                 tCATTCCGAGTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGCATGGCTTTTCCTTTTTTAAAACATGAGGCAAGCCAGTTAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                          <  1:489331/140‑1 (MQ=255)

                                                                                 tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                          <  1:2722212/140‑1 (MQ=255)

                                                                                 tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                          <  2:4907237/140‑1 (MQ=255)

                                                                                 tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                          <  2:3689020/140‑1 (MQ=255)

                                                                                 tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                          <  1:882316/140‑1 (MQ=255)

                                                                                 tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                          <  2:1478260/140‑1 (MQ=255)

                                                                                 tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAAGGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                          <  1:7099656/140‑1 (MQ=255)

                                                                                     tCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATggg                                                      <  2:2561392/140‑1 (MQ=255)

                                                                                         aCTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCGTTTCCCTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCAAGCAAGTACACTCCCTGCAAGGGGGaa                                                  >  2:6875419/1‑140 (MQ=255)

                                                                                             taCGGGCGTTTTCGGATTTTTGGTTGAAGTTTTGGGAGATTTGAATTGGGTTTCCGTTTTTAAAAAAAGAGGCCAGCCAGATAAACCCGACAACAAAAAAAAATAATCTAGCAAGTAAACACCAAGCATGGGGTAaatca                                              >  1:7690811/1‑140 (MQ=255)

                                                                                                  gCGTTTTCGGATTTTTGTTTGAATTTTTTGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCCACAAAAACAAAAAATAATATAGCAAGTAAACTCCATGCATTGGGTAAATAATTAGt                                         >  2:900285/1‑140 (MQ=255)

                                                                                                   cGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTTAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGtt                                        >  2:5940255/1‑140 (MQ=255)

                                                                                                   cGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAAAAAAAAATAATCTAGCAAGTAAAATCCATGCAAGGGGTAAATAATTAGtt                                        >  2:6775457/1‑140 (MQ=255)

                                                                                                       ttCTGATTTTTGTTTGTAGTTTTGGGATATTTGTATGGATTTTCCGTTTTTAAAACAAGAGGCAATCGATATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATTCATGGGGTAATTCATTCGTTGCaa                                    <  1:4606376/140‑1 (MQ=255)

                                                                                                       ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                    <  1:2289379/140‑1 (MQ=255)

                                                                                                       ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                    <  2:2375521/140‑1 (MQ=255)

                                                                                                       ttCGGATTTTTGTTGGCAGTTTTGGGAGAATTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                    <  1:4726251/140‑1 (MQ=255)

                                                                                                                ttgtttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATaaaa                           >  2:223714/1‑140 (MQ=255)

                                                                                                                       aaGTTTTGGGAGATTTGACTGGCTTTTCCTTTTTTAAAACAAGTGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGt                    <  1:7165494/140‑1 (MQ=255)

                                                                                                                       aaGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGt                    <  1:6040466/140‑1 (MQ=255)

                                                                                                                       aaGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGt                    <  2:7526907/140‑1 (MQ=255)

                                                                                                                            ttGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTa               <  2:518572/140‑1 (MQ=255)

                                                                                                                            ttGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTa               <  2:4005074/140‑1 (MQ=255)

                                                                                                                              gggAGCTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAAt             <  2:3222147/140‑1 (MQ=255)

                                                                                                                                 agaTTTGCATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAAtgtg          <  1:2448300/140‑1 (MQ=255)

                                                                                                                                   aTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGcc        <  2:3113724/140‑1 (MQ=255)

AAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGAT  >  NZ_CP034943/240072‑240348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`