Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 316,303 A→C 5.0% K41N (AAA→AAC opuAA → glycine/proline betaine ABC transporter ATP‑binding protein OpuAA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943316,3030AC 5.0% 1533.2 / 8.5 625K41N (AAA→AACopuAAglycine/proline betaine ABC transporter ATP‑binding protein OpuAA
Reads supporting (aligned to +/- strand):  ref base A (321/241);  new base C (3/59);  total (324/301)
Fisher's exact test for biased strand distribution p-value = 5.47e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 631 total aligned reads displayed.

TATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGCTTATTGAACCGACTGCCG  >  NZ_CP034943/316167‑316442
                                                                                                                                        |                                                                                                                                           
     aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCTAGAAAGTAGCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGAATGCCAACGGAAAGAAAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                     >  1:3685631/1‑140 (MQ=255)
     aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCCACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                     >  2:2353146/1‑140 (MQ=255)
     aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                     >  1:2477572/1‑140 (MQ=255)
     aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                     >  1:1005972/1‑140 (MQ=255)
     aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                     >  2:3194947/1‑140 (MQ=255)
     aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                     >  2:4692470/1‑140 (MQ=255)
     aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                     >  1:734146/1‑140 (MQ=255)
     aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                     >  1:234399/1‑140 (MQ=255)
     aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                     >  1:2182561/1‑140 (MQ=255)
     aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                     >  1:6231683/1‑140 (MQ=255)
     aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                     >  2:7686485/1‑140 (MQ=255)
     aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                                                                     >  1:5889598/1‑140 (MQ=255)
     aGGGAAAATATGAGTGTAGATCAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGCAAGACAAAAAAAGAGGTCCTGAAAGCGACCgg                                                                                                                                     >  2:633103/1‑140 (MQ=255)
           aaTATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAAc                                                                                                                               >  1:865819/1‑140 (MQ=255)
                   tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                                                                       >  1:2538911/1‑140 (MQ=255)
                   tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                                                                       >  2:4273081/1‑140 (MQ=255)
                   tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                                                                       >  1:307965/1‑140 (MQ=255)
                   tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                                                                       >  2:3118825/1‑140 (MQ=255)
                   tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                                                                       >  1:613370/1‑140 (MQ=255)
                   tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGAGCAACCGGTgggg                                                                                                                       >  2:6637307/1‑140 (MQ=255)
                   tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGt                                                                                                                      >  1:1185859/1‑140 (MQ=255)
                    gtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGt                                                                                                                      >  2:7252059/1‑140 (MQ=255)
                     tAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAACATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTa                                                                                                                     >  2:6998939/1‑140 (MQ=255)
                      aGATGATAAACCAATTAATATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAACCAGTTCAAATGCTTGCCACCGGAAAGAAAAAAAACGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTaa                                                                                                                    <  2:3357406/140‑1 (MQ=255)
                        aTGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAAt                                                                                                                  >  2:5784582/1‑140 (MQ=255)
                        aTGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAAt                                                                                                                  >  1:4911833/1‑140 (MQ=255)
                        aTGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGCAAACAAACAAATAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGACCCTGAAAGCGACCGGATCAACCGTTGGGGTAAAt                                                                                                                  <  2:1329233/140‑1 (MQ=255)
                           agaAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGACACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTGGGGGTAAATCaa                                                                                                               >  2:645807/1‑140 (MQ=255)
                           agaAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCaa                                                                                                               >  2:2608422/1‑140 (MQ=255)
                           agaAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAAACGGTGGGGTAAATCaa                                                                                                               >  2:5951780/1‑140 (MQ=255)
                             aaaaaaaTTAATATAAAAGTCTATAAATTATATAAAATTTTTGGCAAACAAAAAAATAAAGCAGTTCAAATGTTTGCAAACTTAAATAAAAAAAAAGTGATCTTTAAATATACATTATAAACCGTTGGGGTAAATCaacc                                                                                                             <  2:2718998/135‑3 (MQ=255)
                             aaaCCAATTAAGATACAAGTCGAGAAAGTATATAAAATTTTTGGGAAACAAACAAAGAACGCATTTAAAAGGCTTGCCAACGGAACGACAAAAAAAGCGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                                             <  2:1719046/140‑1 (MQ=255)
                             aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAATCAGTTAAAATGATTGCCAACGGAAAGACAAAAAAATAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                                             >  1:5183923/1‑140 (MQ=255)
                             aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAATAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                                             >  1:6782982/1‑140 (MQ=255)
                             aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGAc                                                                                                                                        >  1:4399811/1‑113 (MQ=255)
                             aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                                             >  1:3420853/1‑140 (MQ=255)
                             aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                                             >  1:1675328/1‑140 (MQ=255)
                             aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                                             >  1:3330801/1‑140 (MQ=255)
                             aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                                             >  1:4040458/1‑140 (MQ=255)
                             aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                                                             >  1:4188934/1‑140 (MQ=255)
                             aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACAGGATCAACCGGTGGGGTAAATCAAGc                                                                                                             >  1:6489042/1‑140 (MQ=255)
                               aCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTGGGGGTAAATCAAGCAg                                                                                                           >  1:4544073/1‑140 (MQ=255)
                               aCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGGTGGGGTAAATCAAGCAg                                                                                                           >  1:7024964/1‑140 (MQ=255)
                                  aaTTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGGTTGCCAACGGACAGACACAAAAAGAGTTCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAtt                                                                                                        >  1:2874583/1‑140 (MQ=255)
                                  aaTTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAtt                                                                                                        >  2:3487558/1‑140 (MQ=255)
                                     taacaaaaacGTCGAGAAAGTAACAAAAATTTTTGGCAAAAAAAAAAAGAAAGCAGTAAAAATGCTTGCCAACGCAAACACAAAAAAATAGAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                                                     <  1:2388529/134‑1 (MQ=255)
                                     tAATATAAAAGTCGATAAATTATCTAAAATTTTTGGCAAACAAACAAAGAAAGCAGTTCAAAGGCTTGCCACCGCAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                                                     <  2:1431284/140‑1 (MQ=255)
                                     tAAGATAAAAGTCGATAACGTATATAAAATTTTTGGCAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAACAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                                                     <  2:4648557/140‑1 (MQ=255)
                                     tAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                                                     <  2:5262838/140‑1 (MQ=255)
                                     tAAGATAAAAGCCGACAAAGTATCTAAAATTTTTGGCAAAAAAAAAAACAACGCAGTTAAAATCCTTCCCAACGCAAACAAAAAAAAAGACATCCGGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                                                                     <  2:378820/140‑1 (MQ=255)
                                       acaTAAAAGTCGACAAATTATCTAAAATTTTGGGCAAACAAAAAAAGAACGCAGTTCAAATGCTTCCCAACGTAAACAAAAAAAAAGACATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                                                                   <  1:2703627/138‑1 (MQ=255)
                                       aGATAAATGTCGATAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                                                                   <  2:420100/140‑1 (MQ=255)
                                       aGATAAAAGTCGCTAAATTATCTAAAATTTTTGGCAAAAAAACAAAGACAGCTGTTCAAATGCTTGCCACCGGAAACAAAAAAAAAGAGATCGTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                                                                   <  2:6389734/140‑1 (MQ=255)
                                            aaaGTCGAGAAAGTATATAAAATTTTGGGTAAAAAAACAAAGAACCCAGTTCAAATGCTTGCCAACGTAAAGAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGtata                                                                                              <  1:5350889/140‑1 (MQ=255)
                                             aaGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTatat                                                                                             >  2:3609125/1‑140 (MQ=255)
                                                  gataatttatataAATTTTTTGGGAAACAAACAAACAAAGCTGTTCAAATGCTTGCCAACGCAAAGAAAAAAAAAGAGATCCTCAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATgg                                                                                        <  1:1850195/133‑1 (MQ=255)
                                                    taatttATCTAAAATTTTTGGGAAACAAACAAATAACCCCGTTCAATGGCTTGCCACCGTAAAGACAAAAAAATAGATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTg                                                                                      <  2:2239098/135‑1 (MQ=255)
                                                          ctCTAAATTTTTTGGGAAACAAAAAACGAACGCAGTTCAAAGGGTTGCCACCGGATAAAAAAAAAAACACATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAAACAAGCAGATTTTGATGTATATGATGGTGAGatat                                                                                <  1:3531997/139‑1 (MQ=255)
                                                          aTCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACCAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGatat                                                                                >  1:7259736/1‑140 (MQ=255)
                                                            atAAATTTTTTGGTAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATAttt                                                                              <  1:5715104/139‑1 (MQ=255)
                                                              aaatTTTTTGGCAAAAAAACAAAGAAAGCAGTAAAAATGCTTGCCAACGGAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGt                                                                            <  2:2537223/140‑1 (MQ=255)
                                                              aaaaTTTTTGGCAAACAAACAAACAAACCAGTAAAAATGCTTGCCAACGTAAACAAAAAAAAAGAGAACCGGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGt                                                                            <  1:6972315/140‑1 (MQ=255)
                                                              aaaaTTTTTGGCAAAAAACCAAACAAAGCAGTTCAAATGCTTGCCAACGGAAACACAAAACAACAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGt                                                                            <  2:5127689/140‑1 (MQ=255)
                                                                   ttttGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGtcatca                                                                       >  2:392395/1‑140 (MQ=255)
                                                                    tttGGGAACCAAACAAAGAAAGCTGTTCAATTGCTTGCCAACGGAAAGACAAAAAAAGTGAGCGGGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                                                                      <  2:1987721/140‑1 (MQ=255)
                                                                    tttGGGAAACAAACACAGAAAGCAGTACAAAGGCTTGCCAACGGAAAGACAAAAAAAGAGATCCGGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                                                                      <  2:112633/140‑1 (MQ=255)
                                                                    tttGGGAAACAAACAAAGAAAGCAGTTCAAAGGCTTGCCAACGGAAACACAAAAAAAGAGAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                                                                      <  2:5673242/140‑1 (MQ=255)
                                                                    tttGGGAAACAAAAAAAGAAAGCATTTCAAATGCTTGCCAACGGAAACACAAAAAAAGAGATCATGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                                                                      <  2:4059997/140‑1 (MQ=255)
                                                                    tttGGCAAAAAAAAAAATAACCCACTAAAAATGATTCCCAACCGAAACAAAAAAAAAGACATCCTCAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                                                                      <  1:5750209/140‑1 (MQ=255)
                                                                     ttGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATg                                                                     >  2:2433454/1‑140 (MQ=255)
                                                                      gggtaaaaaaacAAAGAAAGCATTTAAAATGCTTCCCAACGCAAACACAAAAAAACACATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                                                                    <  1:3844303/136‑1 (MQ=255)
                                                                       ggcaaacaaacaaacAAAGCATTTCAAATGCTTGCCAACGGAAACAAAAAAAAGAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGt                                                                  <  1:5011342/137‑1 (MQ=255)
                                                                        gcaaacaaacACAGAAACCAGTTAAAATGCTGGCCAACGCAAACACAAAAAAACAGAGCCCGAAAGCGACCGGATCAACCGGTGGGGTAAATCAAGCAGATTTTGATTTATATGATGGTGAGATCTTTGTCATCATGGGt                                                                  <  2:456935/138‑1 (MQ=255)
                                                                        gcaaacaaacAAAGAACGCAGTCAAAATGCTTGCCAACCCAAACACAAAAAAAGAGCTCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGt                                                                  <  2:1200963/138‑1 (MQ=255)
                                                                                        agCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATGTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCATGGAGCGGTa                                                  >  1:4631569/1‑140 (MQ=255)
                                                                                           actgcAAATGCTTGCCACCGGAAAGACAACAAAAGAGATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAAt                                               <  2:6934002/136‑1 (MQ=255)
                                                                                            gTTCAAATGCTTGCAAACGGAAAGACAAAAAAAGAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc                                              <  2:4403462/140‑1 (MQ=255)
                                                                                               cataTGCTTGCCACCGCAAACAAAAAAAAAGAGATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAAc                                           <  1:6361336/137‑1 (MQ=255)
                                                                                               cAATTGCTTCCAAACGTAATGAAAAAAAAAGTGTTCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAAc                                           <  2:613370/140‑1 (MQ=255)
                                                                                               aaactGCTGGCCACCGTAAACAAAAAAAAAGAGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAAc                                           <  1:1980828/136‑1 (MQ=255)
                                                                                                      ttGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                                                                   >  1:1458393/1‑77 (MQ=255)
                                                                                                       tccaaaCGCACAGACAAAAAAAGAGAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTAc                                   <  1:7611642/136‑1 (MQ=255)
                                                                                                       tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCTGGGGTGTATAAGGGAGCGGTAAATCAACTTTAGTAc                                   >  2:7755768/1‑140 (MQ=255)
                                                                                                       tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGTTAAATCAACTTTAGTAc                                   >  2:5040741/1‑140 (MQ=255)
                                                                                                       tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTAc                                   >  1:738599/1‑140 (MQ=255)
                                                                                                       tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTAc                                   >  2:2044794/1‑140 (MQ=255)
                                                                                                       tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAGGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTAc                                   >  1:5590961/1‑140 (MQ=255)
                                                                                                       tGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAGTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGTGAGCGGTAAATCAACTTTAGTAc                                   >  1:130778/1‑140 (MQ=255)
                                                                                                       tGCCAACGCAAAGACAAAAAAAGAGAACCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTAc                                   <  2:3029725/140‑1 (MQ=255)
                                                                                                       cgCCAACTCAAACACAAAAAAAGAGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTAc                                   <  1:3204406/139‑1 (MQ=255)
                                                                                                                 atgACAAAACAAGATATCCTGAAAGCGACCGGATCAACCGTTGGCGTAAATCAAGCAGTTTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTTTTaaa                         <  2:5462186/138‑1 (MQ=255)
                                                                                                                 aaGAAAAAAAACGAGATCCGCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTaaa                         <  2:6760179/140‑1 (MQ=255)
                                                                                                                 aaGAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTaaa                         <  1:97170/140‑1 (MQ=255)
                                                                                                                  aGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATTATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAAc                        <  2:7144798/140‑1 (MQ=255)
                                                                                                                    aaaaaaaaaCAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCg                      <  1:7164075/138‑1 (MQ=255)
                                                                                                                    aCAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGGTGGGGTAAATCAAGCAGATTTGGCTGTATATGATGGTGAGATATTTGTCATCAGGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTCAACCg                      >  2:4927017/1‑140 (MQ=255)
                                                                                                                     aaaaaaaaGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCgg                     <  2:4356245/139‑1 (MQ=255)
                                                                                                                        aaaaaGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGCtt                  >  2:7501154/1‑140 (MQ=255)
                                                                                                                                aTCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGCTTATTGAAcc          >  2:5549192/1‑140 (MQ=255)
                                                                                                                                   ctcaaaGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGCTTATTGAACCGAc       <  2:2848105/137‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                           
TATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAACTTTAGTACGGCTGTTAAACCGGCTTATTGAACCGACTGCCG  >  NZ_CP034943/316167‑316442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: