Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 112,586 G→T 8.0% intergenic (+264/‑40) gltX → / → cysE glutamate‑‑tRNA ligase/serine O‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943112,5860GT 8.0% 1462.3 / 20.7 596intergenic (+264/‑40)gltX/cysEglutamate‑‑tRNA ligase/serine O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (161/361);  new base T (68/4);  total (229/367)
Fisher's exact test for biased strand distribution p-value = 1.41e-26
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 610 total aligned reads displayed.

TTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTAC  >  NZ_CP034943/112447‑112725
                                                                                                                                            |                                                                                                                                           
   cGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGt                                                                                                                                          <  2:2492125/140‑1 (MQ=255)
        tCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAaga                                                                                                                                     <  2:2238970/140‑1 (MQ=255)
        tCAGTAGTCCATGGCCGCCGCCGCCGGTACAGGCTGAAGTGGGGACCGCAGAAGCCTGCTTTTCAACCAGCGTCGAACCGCGCGGTTTAAGCGTCTCAGGCATGTTTTCATGGAGAGGCGCTTTTTTTATTGGGTGAaga                                                                                                                                     <  2:1001121/140‑1 (MQ=255)
             aTTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGaaa                                                                                                                                <  2:5192701/140‑1 (MQ=255)
               tCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATc                                                                                                                              <  1:715126/140‑1 (MQ=255)
               tCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATc                                                                                                                              <  1:3207549/140‑1 (MQ=255)
                      cggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAATCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAACAGGCAATCAAAAaga                                                                                                                       >  1:295208/1‑140 (MQ=255)
                       ggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATagag                                                                                                                      <  1:1142807/140‑1 (MQ=255)
                        gccgccgccGTTACAGGCTGAAGGTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAACAGGAAATCAAATAgtga                                                                                                                     >  2:261493/1‑140 (MQ=255)
                                     ctggCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGc                                                                                                        <  1:55823/138‑1 (MQ=255)
                                       ggCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCAt                                                                                                      <  2:842275/140‑1 (MQ=255)
                                       ggCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCAt                                                                                                      <  1:5860431/140‑1 (MQ=255)
                                            aaGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTTGGTGGAGTGGTAATGCAATATAGAAGTGGGGGGTAGCATGTGtt                                                                                                 >  1:302051/1‑140 (MQ=255)
                                                  ggAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGTAGAGGAAAACAAAAAGAGAAATGGGGGGAAGCATGTGTTTTTTat                                                                                           >  1:2741077/1‑139 (MQ=255)
                                                   gagaGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGa                                                                                          <  1:7435922/140‑1 (MQ=255)
                                                   gagaGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGa                                                                                          <  1:2222653/140‑1 (MQ=255)
                                                   gagaGCACATGTATGCGTTTCAAAAAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCCGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGa                                                                                          <  1:3357594/140‑1 (MQ=255)
                                                      agCAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGGAAGAGGCAATCAAAAATAGAACTGGGGGGAAGCCTGTGTTTTTTAGAAtt                                                                                       >  1:828493/1‑139 (MQ=255)
                                                          taaGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCa                                                                                   <  1:7741665/139‑1 (MQ=255)
                                                             gTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGACATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaag                                                                                <  1:1601463/140‑1 (MQ=255)
                                                             gTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGGAAGACGAAAACAAAAAGAGAACTGGGGGGAACCATGTGTTTTTTAGAATGCTCacac                                                                                >  1:4433707/1‑137 (MQ=255)
                                                             ctCTGCTTTTCAAACAGAGTCGAACCGCGCGGTTTAAGAGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaag                                                                                <  2:4579365/139‑1 (MQ=255)
                                                               cggCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTGTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                              <  2:4446321/138‑1 (MQ=255)
                                                               cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                              <  2:4806218/140‑1 (MQ=255)
                                                               cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                              <  2:6797173/140‑1 (MQ=255)
                                                               cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                              <  2:3243011/140‑1 (MQ=255)
                                                               cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                              <  2:4695253/140‑1 (MQ=255)
                                                               cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                              <  1:2486493/140‑1 (MQ=255)
                                                               cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                              <  1:4853146/140‑1 (MQ=255)
                                                               cTGCTTTTCAAACAGAGTGGAACCGCGCGGGTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                              <  2:6417673/140‑1 (MQ=255)
                                                               atGCTTTTCAAACAGAGTGGAACCGCGCGCTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAACCATGTGTTTTTTAGAATGCTCAaagaa                                                                              <  2:74/139‑1 (MQ=255)
                                                                 gCTTTTCAACCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                            <  2:4914136/140‑1 (MQ=255)
                                                                 gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGCAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                            <  1:1441368/140‑1 (MQ=255)
                                                                 gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagacga                                                                            <  1:6510617/140‑1 (MQ=255)
                                                                 gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                            <  2:2712284/140‑1 (MQ=255)
                                                                 gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                            <  2:1127348/140‑1 (MQ=255)
                                                                 gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                            <  1:1851217/140‑1 (MQ=255)
                                                                 gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                            <  1:926463/140‑1 (MQ=255)
                                                                 gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                            <  1:2618038/140‑1 (MQ=255)
                                                                 gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                            <  2:7229615/140‑1 (MQ=255)
                                                                 gCTTTTCAAACAGAGGGGAACCTCGCGGTTTAAGCGTCTCTGTCATGATGTCATGCAGAGGCGCTTTTTGTAGTGGGTTACGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGATTTTTAGAATGCTCAAagaaga                                                                            <  2:6141755/140‑1 (MQ=255)
                                                                   ttttCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACa                                                                          <  1:5012528/140‑1 (MQ=255)
                                                                     ttCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGACAAGTTGGGGGAAGCATTTGTTTTTTAGAATTATCAAataagaaaat                                                                        >  2:2039993/1‑136 (MQ=255)
                                                                       cAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTTTTTTTTTTGGGGGAGAGGGAATCAAATATAGTAGTTGGGGGGAGCAGGGGTTTTTTATAATTCTCAAAGAAAACaattt                                                                      >  1:4670348/1‑136 (MQ=255)
                                                                         aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGCCATTGATa                                                                    <  2:3583923/140‑1 (MQ=255)
                                                                         aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                    <  1:3946359/140‑1 (MQ=255)
                                                                         aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                    <  2:7679113/140‑1 (MQ=255)
                                                                         aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                    <  2:5663494/140‑1 (MQ=255)
                                                                         aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                    <  2:1896063/140‑1 (MQ=255)
                                                                         aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                    <  2:3142293/140‑1 (MQ=255)
                                                                         aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGGCAGAGGAAATCAAATAGAGAAGCGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                    <  2:6572618/140‑1 (MQ=255)
                                                                           cAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACa                                                                  <  2:412932/140‑1 (MQ=255)
                                                                             gagTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTTTTTTATTTGGTGAAGAGGAACTCAAATATAGTAGGGGGGGGCAGCCTGTGTTTTTTAGTATGCGGAAAGTAGACCTTTATACact                                                                >  1:7072338/1‑138 (MQ=255)
                                                                              agTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGAAGAGGAAATAAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTTGAATTCTCAAAGAACACATTGATAAAgtg                                                               >  2:5924281/1‑140 (MQ=255)
                                                                                tGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGtt                                                             <  2:2091186/140‑1 (MQ=255)
                                                                                tGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATAAAATAGAGAAGTGGGGGGAAGCATGTTTTTTTTATAATGCTCAAAGAAGACATTTATAAAGTGtt                                                             >  2:2394502/1‑140 (MQ=255)
                                                                                tGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGAGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGtt                                                             <  2:4336283/140‑1 (MQ=255)
                                                                                tGGAACCGCGCGGTTTAAGCGTCTCGGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGtt                                                             <  2:3319375/140‑1 (MQ=255)
                                                                                        cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGCGGCGCTTTTTTTATTTGGGGTAGACGAAATGAAATATTGAATGGGGGGGACGCCTGTGTTTTTTTGAATTCTGTAAGAAACAATTGATACCGTGTTAGGTCAAg                                                     >  1:100097/1‑140 (MQ=255)
                                                                                        cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTTGGTGAAGAGGAAATCAAATAGAGTAGGGGGGGGAAGCAGGTGTTTTTTAGAATGCTCAAAGAAGAACTTGATAAATTGTTCGATCAAg                                                     >  2:6668095/1‑140 (MQ=255)
                                                                                        cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTGGGTGGAGAGGAAAAAAAATATAGAAATGGGGGGAAGCATGTGTTTTTTAGTATGCTCAAAGAAGACATATATAAACTGTTAGATCaaa                                                     >  1:5703886/1‑139 (MQ=255)
                                                                                        cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGTAGAGGAAATCAAATATAGAAGTGGGGGGCAGCATTTGTTTTTTTGAATTCTGAAAAAAGACAATGATAAAATGTTCGATCaat                                                     >  1:5173421/1‑139 (MQ=255)
                                                                                        cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGAAGAGGAAATCAAATATAGAACTGGGGGGAAGCCTGTGTTTTTTAGAATGCTAAAAGAAGACATTGATACACTGTTCGaaaaaa                                                     >  1:5953952/1‑135 (MQ=255)
                                                                                        cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGAAGAGGAAAACAAAAAGAGAAGTGGGGGGAACCCAGTGTTTTTTATTATTCTCAAATAAAAAATTGATAAAGTGTTCGATAAAg                                                     >  1:7504877/1‑140 (MQ=255)
                                                                                        cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGTGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATTCTCAAAGAAGACATTGATACAGTGTTAGATCAAg                                                     >  2:5136207/1‑140 (MQ=255)
                                                                                        cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAg                                                     >  2:6992939/1‑140 (MQ=255)
                                                                                        cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAAAATAGAAGTGGGGGGAAGCATGTGTTTTTTAGAAAGCTCAAACAAGACATTGATACAGTGTTCGATCAAg                                                     >  1:7624852/1‑140 (MQ=255)
                                                                                        cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATAAAAAAGAGAATTGGGGGGAACCATGTGTTTTTTTGAATGCGCAAAGAAGACAATGATACAGTGTTAGATaaat                                                     >  1:4275233/1‑139 (MQ=255)
                                                                                        cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGGAAGAGGAAATCAAATAGAGAAGGGGGGGGAAGGATGTGTTTTTTAGAATGCTCAAAGAAGACAATGATACCGTGTTCGATCAAg                                                     >  2:2805436/1‑140 (MQ=255)
                                                                                          cgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTTGGTGTAGATGAAATAAAATAGATAACTGGGGGGAAGCATGTTTTTTTTATAATGCTGAAATAAGAAAATTTTACAATGTTAGATAAAGAt                                                   >  1:6638476/1‑140 (MQ=255)
                                                                                             tttAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGGGAAGAGGAAAACAAATAGAGAACTGGGGGGAAGCCTTTGTTTTTTAGAATGCTCAAATAACACAATTAAACAGGGTTCGATCAATATAca                                                >  1:6111689/1‑140 (MQ=255)
                                                                                                       tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTTGGGGAAGAGGAAATAAAAAACAGAAGTGGGGGGAAGCATGTGTTTTTTATAATTCTCAAAGAACACAATTATAAAGTGTTAGATCAAGATACATCACCaagaa                                      >  1:3568391/1‑140 (MQ=255)
                                                                                                       tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGTAGAGGGAAACAAATATTGAAGTGGGGGGGAGCCGGTGTTTTTTATAATTCTGAAACAATACATTTATAAAGTTTTCGATAAAGATACAGCAGCaagaa                                      >  1:6264141/1‑140 (MQ=255)
                                                                                                       tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTATAATTCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaa                                      >  2:7167810/1‑140 (MQ=255)
                                                                                                       tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAATAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaa                                      >  2:5409374/1‑140 (MQ=255)
                                                                                                       tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAACCAGGTTTTTTTTATAATGCTCAAAGAAGACATTGATAAAGTGTTCGATCAAGATCAAGCAGCaagaa                                      >  2:6254236/1‑140 (MQ=255)
                                                                                                       tctGTCATGTTTTCATGCAGAGGCGCGTTTTTTTTTTGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCCTGTGTTTTTTTTAATGGTCAAAGAAGACATTGGTAAAGTGTTCGTTAAAGATCAAGCAGCaagaa                                      >  1:6905267/1‑140 (MQ=255)
                                                                                                       tctGTCATGTTTTCATGCAGAGGCGCGTTTTTTATTTGGTGTAGAGGCAAAAAAAAATATAAGTTGGGGGACGCATGTGTTTTTTTTAATTCTCAAAAAAGAAAATGAAAAAGTGTTCGATAAAAATACAGCAGCaaaaa                                      >  1:1280956/1‑140 (MQ=255)
                                                                                                       tctGTAATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAATAGGAAATCCATTAGATAAGTGGGGGTAACCAGGTGTTTTTTAGAATTCTAAATGTAGACATTTATACAGTGTTCGAGGAAGATACCGCAGCtagta                                      >  2:1319332/1‑138 (MQ=255)
                                                                                                                  ttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCACATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGaa                           <  1:3274549/140‑1 (MQ=255)
                                                                                                                  ttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTTTAATGCTCAAAGAAGACAATGATAAAGTGTTCGATCAATATACAGCAGCAAGAAGTTATTTTGaa                           >  2:1238271/1‑140 (MQ=255)
                                                                                                                  ttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGaa                           <  2:6035370/140‑1 (MQ=255)
                                                                                                                  ttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGaa                           <  2:5038738/140‑1 (MQ=255)
                                                                                                                  ttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGaa                           <  1:6830291/140‑1 (MQ=255)
                                                                                                                  ttCATGCAGAGGCGCTTTTTTTATGGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGaa                           <  2:4957599/140‑1 (MQ=255)
                                                                                                                  ctCAGGCAGAGGCGCTATCTGTATGGGGCGACCCCGAACTCCCAGAGAGAAGTGGCGGGAAGCAAGGGTTTTTGAGAATGCTCAAAGAAGACATTGATACCGGGTTCGAGCAAGATCCAGCAGCAAGAAGTTATTTTGaa                           <  2:1777778/139‑1 (MQ=255)
                                                                                                                   tCATGCAGAGGCGCTTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGTAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGaa                           <  1:1778787/140‑1 (MQ=255)
                                                                                                                     aTGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTg                        >  2:3436589/1‑140 (MQ=255)
                                                                                                                     aTGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAAACCAATAGAGAAGTGGGGTGAAGCATGTGTTTTTTAGAAAGCTCAAAGAAAAAATTGATACAGTGTTAGATAAATATCCATCAGCAAGAAGTTATTTTGAATTg                        >  2:3746871/1‑140 (MQ=255)
                                                                                                                                    tttttATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTc          <  1:3672659/140‑1 (MQ=255)
                                                                                                                                     ttttATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTcc         <  1:1982525/140‑1 (MQ=255)
                                                                                                                                        tATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGACGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGt      >  2:5561190/1‑140 (MQ=255)
                                                                                                                                        tATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGt      <  1:5435897/140‑1 (MQ=255)
                                                                                                                                           tGGGTGAAGAGGAAATCAAATAGAGCAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa   <  2:3665941/140‑1 (MQ=255)
                                                                                                                                           tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa   <  2:4253692/140‑1 (MQ=255)
                                                                                                                                           tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa   <  2:295248/140‑1 (MQ=255)
                                                                                                                                           tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa   <  2:7503797/140‑1 (MQ=255)
                                                                                                                                           tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGATTa   <  2:1463442/140‑1 (MQ=255)
                                                                                                                                            gggTGAAGCGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTAc  <  1:3478619/140‑1 (MQ=255)
                                                                                                                                            gggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTAc  <  1:4093397/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTAC  >  NZ_CP034943/112447‑112725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: