Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 549,085 C→A 11.7% Q33K (CAA→AAA)  EO946_RS02865 → PLP‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943549,0850CA11.7% 1052.5 / 26.9 443Q33K (CAA→AAA) EO946_RS02865PLP‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base C (208/164);  new base A (3/68);  total (211/232)
Fisher's exact test for biased strand distribution p-value = 4.51e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 443 total aligned reads displayed.

GTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCT  >  NZ_CP034943/548947‑549222
                                                                                                                                          |                                                                                                                                         
   ttCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCaaaa                                                                                                                                       <  1:1367683/140‑1 (MQ=255)
   ttCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCaaaa                                                                                                                                       <  1:3257319/140‑1 (MQ=255)
             aaTGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAg                                                                                                                             <  1:6726068/140‑1 (MQ=255)
             aaTGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAg                                                                                                                             <  1:3708696/140‑1 (MQ=255)
                gCATACGAGTTAGGAGAACAACCCAAATTGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTc                                                                                                                          <  2:5994468/140‑1 (MQ=255)
                gCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTc                                                                                                                          <  1:7756933/140‑1 (MQ=255)
                gCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTc                                                                                                                          <  2:4424443/140‑1 (MQ=255)
                gCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTc                                                                                                                          <  2:2229625/140‑1 (MQ=255)
                         ttAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCaaa                                                                                                                 >  1:74181/1‑140 (MQ=255)
                          tAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCaaaa                                                                                                                <  1:360561/140‑1 (MQ=255)
                          tAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCaaaa                                                                                                                <  1:3121531/140‑1 (MQ=255)
                           aGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAg                                                                                                               >  1:6190471/1‑140 (MQ=255)
                                aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCCAAAGACTcc                                                                                                          >  2:518239/1‑140 (MQ=255)
                                aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGa                                                                                                              >  2:2617008/1‑136 (MQ=255)
                                aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGa                                                                                                              <  1:2617008/136‑1 (MQ=255)
                                aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTcc                                                                                                          >  1:3834777/1‑140 (MQ=255)
                                aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTcc                                                                                                          >  2:5118730/1‑140 (MQ=255)
                                aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTcc                                                                                                          >  2:1378306/1‑140 (MQ=255)
                                aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTcc                                                                                                          >  1:6840459/1‑140 (MQ=255)
                                  caacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCACAAGACTCCTg                                                                                                        >  2:863515/1‑140 (MQ=255)
                                  caacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTg                                                                                                        >  2:231869/1‑140 (MQ=255)
                                   aacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGTAGCTCCCTTACAAAAGACACCTgg                                                                                                       >  2:7683560/1‑140 (MQ=255)
                                        acaTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGt                                                                                                  <  1:2992702/138‑1 (MQ=255)
                                        aaaTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGt                                                                                                  <  1:5966028/140‑1 (MQ=255)
                                          aTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAATGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGACGCTCCCTTCCCAAAGACTCCGGGCGAGTCa                                                                                                >  1:6354693/1‑140 (MQ=255)
                                             gATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCt                                                                                             >  2:5467150/1‑140 (MQ=255)
                                             gATATCACGCCTTTTTTGAATAGAAATCGGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCt                                                                                             >  1:5343885/1‑140 (MQ=255)
                                               tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                           >  1:1677553/1‑140 (MQ=255)
                                               tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                           >  1:4854090/1‑140 (MQ=255)
                                               tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                           >  1:1240980/1‑140 (MQ=255)
                                                atCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTc                                                                                          >  2:6492555/1‑140 (MQ=255)
                                                atCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCATGGCGAGTCAGCTTTc                                                                                          >  2:1759854/1‑140 (MQ=255)
                                                  cACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCa                                                                                        <  1:5555868/140‑1 (MQ=255)
                                                  cACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCa                                                                                        >  1:7287140/1‑140 (MQ=255)
                                                    cGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                                                      >  2:5367051/1‑140 (MQ=255)
                                                    cGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                                                      >  1:952917/1‑140 (MQ=255)
                                                      ccTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTCTTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAg                                                                                    >  2:2446837/1‑140 (MQ=255)
                                                       cTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAACAGACTCCTGGCGAGTCAGCTTTCCATCAGt                                                                                   >  2:7497143/1‑140 (MQ=255)
                                                       cTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGt                                                                                   >  2:3721806/1‑140 (MQ=255)
                                                       cTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGt                                                                                   >  1:4616166/1‑140 (MQ=255)
                                                       cTTTTATGAATAGAAATCTGGATATTCCGCTTTATCAGAAGCATTATCGCTATTTCAAACAAAACATGAATCAGGCGCGTATTCAAAAAGCAATGAAGCTCACTTCCAAAAGACTCCTGGCGAGTCAGCTTTACATCAGt                                                                                   >  2:6672145/1‑140 (MQ=255)
                                                         tttttGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCa                                                                                 >  2:3519745/1‑140 (MQ=255)
                                                         tttttGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGATACATTCAAAAAGACTCCTGGAGAGTCAGCTATGCATCAGACa                                                                                 >  2:4566355/1‑140 (MQ=255)
                                                          ttttGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCACAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCaa                                                                                >  2:6154590/1‑140 (MQ=255)
                                                           tttGAATAGAAATCTGTATATTCCGCTTTATCCGCAGCTTTATCGATTTTTCAAAGAAAACATCCATCAGGGGCGTATTCAAAAAGGAATGACGCTCCCTTCCAAACCACGCCTGGCGAGTCAGCTTTCCATCCGTCaca                                                                               <  2:5858182/140‑3 (MQ=255)
                                                            tttaaTATAAATTTTGATTTTCGGATTTATCAGCTGCTTTATCGATATTTAAAATAAAACTTGATTCAGGGGCGTTTTAAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAAc                                                                              <  2:1594575/137‑1 (MQ=255)
                                                            tttaaTATAAATCTGTATATTCCGGTTTATCAGAAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGTAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAAc                                                                              <  2:5940387/137‑1 (MQ=255)
                                                            tgcaaTAGAAAACTGGATATTCCGCTTAAACAGCCGCTTTATCGATATTTCAACAAAAACATGCATCAGGGGCGTTTTCAAAAAGGAATGACGCTCCCTTCCAACCCACTCCGGGCCAGTCCGATTTCCATCCGTCAAAc                                                                              <  2:1845564/137‑1 (MQ=255)
                                                                 tAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTg                                                                         >  2:3773538/1‑140 (MQ=255)
                                                                   gAAATCTGGATATTCCGCTGTCTCAGCCGGTTTATCGCTATGACAAAGAAACCATGCATCTGGGGCTTTTTCAAAAAGGAATGACGCGCCCTCCCAAAAGACTCCTGGCGAGTACGTTTCCCAACAGGCACACGACTGTa                                                                       <  2:3286883/140‑1 (MQ=255)
                                                                        gtGGATATTCCGCTTTATCCGCTGCTTTATCGATATTAAAAACAAACCATGCATCCGGGGCGTATCCAAAAAGCAATGAAGCTCCCTTCAAACACCCGCCTGGCGAGTCAGCTTTCAAACAGGAAAACGACTGTAGAACg                                                                  <  2:2923431/139‑1 (MQ=255)
                                                                        cggtatatTCCGCTTTATCCGCAGCTTTATCGCTATTGCAAACAAAACATGCAGCAGGGGGGTATTCAAAAAGCAATGAAGCGCCCTTCCAAACGACGCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACg                                                                  <  1:4148305/136‑1 (MQ=255)
                                                                        cgggATATTCCGCTTTTTCAGCAGATTTATCGCTATTTCAAATAAAACATGCATCCGGGGCGTATTAAAAAAGGAATGAAGCTCCCTTCCAAACGACGCGGGGCGAGTCAGGTTTCCATCAGTCAAACGACTGTAGAACg                                                                  <  2:497184/138‑1 (MQ=255)
                                                                        cTGTATATTCCGCTTTATCCGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCCGGGGCTTTTACAAAAAGGAAGGACGCGCCCTTCCAAAAGACGCCGGGGGCGTCCGCTTTCCATCAGACACACGACTGTAGAACg                                                                  <  1:1579847/140‑1 (MQ=255)
                                                                        cTGTATATTCCGATTTACCCGAAGATTTATCGCTATTTCAAACAAAACATGCAGCCGGGGCTTATTAAAAAAGTAATGAAGCTCCCTACCAAACGCCGCCTGGCGAGTCAGCTTTCCATCAGACAAACGACTGTAGAACg                                                                  <  1:231869/140‑1 (MQ=255)
                                                                        cTGGATATTCCGCTTTAGCCGCAGCTTTAGCGATATTCCAAATAAAACAGGCATCCGGGGCGTATTCAAAAAGGAATGACGCGCCCTTCCAAAAGCCTCGGGGCGCGACAGCTTCCCATCAGTCAAACGACTGTAGAACg                                                                  <  2:6509890/140‑1 (MQ=255)
                                                                         tGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGt                                                                 >  2:7293905/1‑140 (MQ=255)
                                                                           gATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGc                                                               >  1:4800892/1‑140 (MQ=255)
                                                                                tCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCCGTCAAACGACTGTCGAACGTGCCTATg                                                          >  1:3974841/1‑140 (MQ=255)
                                                                                tCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATg                                                          >  1:2812304/1‑140 (MQ=255)
                                                                                tCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATg                                                          >  1:7631559/1‑140 (MQ=255)
                                                                                  cgatttATCTGAAGCTTTATCTATATTTCAAATAAAACATGAATCTGGGGGTTATTCAAAAATTAATGAAGCTCGCTTCCAAAAGACTCCTGGCGTGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGaa                                                        <  2:4635547/137‑1 (MQ=255)
                                                                                  cgatttATCAGCTGCTTTATCGATTTTTAAAAGAAAACATGAATCAGGGGCGTATTCAAAAAGTATTGACGCTCCCTCCCAAAACACGCCGGGCGATTCAGCTTACAATCAGACAAACGACTGTAGAACGTGCCTATGaa                                                        <  1:7541748/137‑1 (MQ=255)
                                                                                   gCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGaaa                                                       >  2:3990954/1‑139 (MQ=255)
                                                                                    cTTTATCAGCAGCTTTATCGATATTTCAAATAAAAAATGCATCAGGGGCTTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCGGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACa                                                      <  2:6575368/140‑1 (MQ=255)
                                                                                       taccagcagTTTTATCGTATTTTCAAACAAAACAGGCAACAGGGGCGTATACAAAAAGGAATCAAGCTCCGTTCCAAAAGACCCCTGGCGAGTCAGCTTTCCATCCCTCACACGACTGTAGAACGTGCCTATGAACAGCt                                                   <  1:5687283/137‑1 (MQ=255)
                                                                                              agttttATCGCTTTTCAAAAGAAAAATTGCATCCGGGGCTTATTAAAAAAGTAATGAAGCTCCCTTCCAAAAGACTCCTGGCGATTCAGCTTTCAATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                            <  2:6344194/137‑1 (MQ=255)
                                                                                              agcTTTATCTATATTACAAACAAAAACTGCATCCGGGGCTTATTAAAAACGGAATGAAGATCCATTCCAAACTCCCCGTGGCGAGTCAGCTTTCAATACGTAACACGACTGTAGAACGTGCCTATGAACATCTTgctgct                                            <  2:3391268/140‑1 (MQ=255)
                                                                                              agcTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCTTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTACCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                            <  2:2106175/140‑1 (MQ=255)
                                                                                              agatttATCGAAAATTCAACTAAAACAGCCATCCGGGCCTTTTACACAACGCACTCAAGCTCCCTTCCAAACCACCCCGGGCGAGTCAGATTCCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCGTgctgct                                            <  2:4817082/137‑1 (MQ=255)
                                                                                              accTTTATCGCTATTTCAAACAAAACATGCATCCGGGGCTTATTAAAAAAGTAATGAAGCTCCCTTCCAAAACACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                            <  2:4205228/138‑1 (MQ=255)
                                                                                                ttttATCGATATTTAAAATAAAACATGCATCTGGGGCGTATAAAAAAAGTAATGAAGCTCCCTACCAAAAGCCTCCGGGCGAGTCTGCTTCCCATCAGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                          <  1:2191263/139‑1 (MQ=255)
                                                                                                ctgaaccGCAATATCAAACAAACCACGAATCAGCGGCGTATTAAAAAAGCAACGCACCTCCCTACCAAACCACACCCGGCCAGTCACCTTACCACCCTCCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                          <  2:4258390/134‑1 (MQ=255)
                                                                                                cTTTCTCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAATGGAATTAAGCTCCATTCAAACCCCCCCGTGGCTAGTCAGCTTGCCAACAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                          <  2:6208720/140‑1 (MQ=255)
                                                                                                cTTTATCGCTATTTAAAACAAAACATGCACCAGGGGCGTATTAAAAAAGGAATGAAGCTCCCTTCCAAAACCCGCCTGGCGCGTCAGCTTTCCAACAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                          <  2:5528705/140‑1 (MQ=255)
                                                                                                cTTTATCGCTATTACAAACAAAACTTGCAACCGGGGCGTATCCAAAACCCATTGAACCCCCATTCCAAAAGACGCGTGGCTAGTCAGCTTGCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                          <  1:517352/140‑1 (MQ=255)
                                                                                                cTTTATCGCTATTACAAACAAAACATGAATCCGGGGCTTATTAAAAAAGGAATGAAGCTCCCTTCAAAAAGACTCCTGGCGAGTCAGCTTTCCACCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                          <  2:6717622/140‑1 (MQ=255)
                                                                                                cTTTATCGCTATTACAAACAAAACATCCATCCGGGGCTTATAAAAAAAGCACTGAAGCTCCCTACCAACACACCCCGGGCGAGTCAGCTTTCCAACAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                          <  1:3375259/140‑1 (MQ=255)
                                                                                                     tcgatttttCAAATAAAACATGAATCCGGGGCTTATTCAAAAAGGAATGAAGCTCCCTTCCAAAACACTCCTGGCGAGTCAGCTTTCAATCACTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCt                                     <  1:6941836/134‑1 (MQ=255)
                                                                                                          aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGGACAGCTTGCTGCTGAGGGCTATATc                                >  1:7005776/1‑140 (MQ=255)
                                                                                                          aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTCTATc                                >  1:1097405/1‑140 (MQ=255)
                                                                                                          aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                >  1:1819560/1‑140 (MQ=255)
                                                                                                          aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                >  1:2043825/1‑140 (MQ=255)
                                                                                                          aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                >  1:1029427/1‑140 (MQ=255)
                                                                                                          aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                >  2:3145786/1‑140 (MQ=255)
                                                                                                             tCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTg                             >  2:3645371/1‑140 (MQ=255)
                                                                                                                aataaaaaaTGAATCTGGGGCTTATAAAAAAAGGAATCAAGCCCCCTTCCAAAACACTCCTGGCGAGACAGCTTTCCATCAGTAACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGc                          <  1:4420508/137‑1 (MQ=255)
                                                                                                                     aaCATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGcc                     >  1:771819/1‑140 (MQ=255)
                                                                                                                              ccggggCTTATTAAAAAAGGAATGAAGCTCCCTTCCAAACGACTCCGGGCGAGTAAGCTTTCCATCAGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCgg            <  1:7155454/138‑1 (MQ=255)
                                                                                                                                 gggCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATg         >  1:5262021/1‑140 (MQ=255)
                                                                                                                                    cGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGtt      >  1:2477277/1‑140 (MQ=255)
                                                                                                                                      tataCAAAAAGGAATGAAGCCCCCTTCCAACACACTCCTGGCGAGTCAGCTTCCCATCACACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg    <  1:6492555/140‑1 (MQ=255)
                                                                                                                                      tATTCAAAAAGGAATTAAGCTCCATTCCAAAAGACTCGTGGCTAGTCAGATTTCCATCAGACAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg    <  2:5641526/140‑1 (MQ=255)
                                                                                                                                      tATTCAAAAAGGAATGAAGCTCCCTTCCAAACGCCGCCGGGCGAGTAAGCTTGCCATCAGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg    <  2:6767189/140‑1 (MQ=255)
                                                                                                                                      tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCACTAAGCGGAGGGGTTg    >  2:4309532/1‑140 (MQ=255)
                                                                                                                                      tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg    >  2:1291560/1‑140 (MQ=255)
                                                                                                                                      tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg    >  2:7119768/1‑140 (MQ=255)
                                                                                                                                      tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg    >  2:3579419/1‑140 (MQ=255)
                                                                                                                                        ttCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCt  >  2:7187478/1‑140 (MQ=255)
                                                                                                                                        ttCAAAAAGGAAGGAAGCTCCCTTCCAACAGACGCCTGGCGAGTCAGCTTTCCATCCTCCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCt  <  2:6907785/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                         
GTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCT  >  NZ_CP034943/548947‑549222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: