BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,413	A→C	11.4%	T86P (ACC→CCC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,413	0	A	C	11.4%	670.2 / 15.1	347	T86P (ACC→CCC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (107/179); new base C (3/51); total (112/235)
Fisher's exact test for biased strand distribution p-value = 8.44e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 355 total aligned reads displayed.

AGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGG  >  NZ_CP034943/607274‑607552

aGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCCACGGCAGCTACCGGGGACGCCGGACAAGCCGGACCaa                                                                                                                                             >  1:6308374/1‑140 (MQ=255)

 gCCACGGGAGCTACCGGCGAAGCTGGACCAGCCGGAGCGACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAc                                                                                                                                            <  2:4955435/140‑1 (MQ=255)

  ccACTGGAGCTACCGGGGAAGCTGGACCTGCCGGAGCGACGCGAGCCACAGGAGCTACCGGGGAAGCGGGACCAGCCGGTCCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAcc                                                                                                                                           <  2:1977831/140‑1 (MQ=14)

  ccACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCCACGGGAGATACCGCGCAAGCCGGACCAGCCGGACCAccc                                                                                                                                           >  1:258998/1‑140 (MQ=18)

    aCGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCgg                                                                                                                                         <  2:110105/140‑1 (MQ=255)

           cTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgaca                                                                                                                                  >  1:625387/1‑140 (MQ=255)

            tACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGCGCCACGGGAGCGACCGGCGCCGCCGGACCAGCCGGAGCCACCggcgcgcccg                                                                                                                                 >  2:6145834/1‑136 (MQ=17)

             aCCGGGGAAGAAGGACCAGCCGGAGGGACGGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACGGGGGAAGCCGGACCAGCCGGACCACCCggagcgacagg                                                                                                                                <  1:1433707/140‑1 (MQ=18)

               cGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggag                                                                                                                              <  1:4905722/140‑1 (MQ=255)

                   gcaGCTGGACCAGCCGGACCTACGGGAGCGAGAGGAGCTACCGGGGAACCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagggacaggagcgac                                                                                                                          <  2:7248687/138‑1 (MQ=17)

                   gAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                                                                          <  2:742633/140‑1 (MQ=255)

                   gAAGCTGGACCAGACGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                                                                          <  2:6707067/140‑1 (MQ=255)

                      gCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacaggagcgacagg                                                                                                                       >  2:2472659/1‑140 (MQ=255)

                      gCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacaggagcgacagg                                                                                                                       >  2:2549788/1‑140 (MQ=255)

                      gCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacaggagcgacagg                                                                                                                       >  2:2850505/1‑140 (MQ=255)

                      gCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacaggagcgacagg                                                                                                                       <  2:625387/140‑1 (MQ=255)

                      gCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacaggagcgacagg                                                                                                                       >  2:695623/1‑140 (MQ=255)

                        gggcccAGCCGGCGCTCCGGGAGCGACCGGAGCTCCCGGGGCAGCCGGACCAGCCGGCCCACCCGGAGCGACGGGAGCCACGGGTGCTCCCGGGGACGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                                                                                     <  2:3791651/136‑1 (MQ=17)

                           tccAGCCGGAGAGCTGGGACCGACAGGAGCTACCGGGGATGCCGGACCACCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCGGGAGCGACCGGAGCGACAGGAGCTa                                                                                                                  <  1:2038859/139‑1 (MQ=17)

                           aCCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCAACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCtc                                                                                                                  >  2:40228/1‑139 (MQ=255)

                               gCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcga                                                                                                                           <  1:7717381/127‑1 (MQ=255)

                                cctgAGCGACGCGAGCGACATGAGCTACTGGGGAAGCCGGACCAGCCGGACCAACCTTAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                             <  2:3528262/137‑1 (MQ=17)

                                    aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         >  1:2914019/1‑140 (MQ=255)

                                         cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                    <  2:4254288/140‑1 (MQ=255)

                                         cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                    >  1:3245621/1‑140 (MQ=255)

                                          gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGCGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACCGGAGATACCGGGGAAGCCGGAc                                                                                                   >  2:5077106/1‑140 (MQ=17)

                                          gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGAGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                   >  2:5559877/1‑140 (MQ=255)

                                              gCGACTGGAGATACCGGGGAAGCCGGACCAGCCGGACCATCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                                               <  1:3897021/140‑1 (MQ=255)

                                              gCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                                               <  1:679252/140‑1 (MQ=255)

                                                 aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                            >  2:5422816/1‑140 (MQ=255)

                                                          aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagg                                                                                   >  2:6812684/1‑140 (MQ=255)

                                                          aCCGGGGAAGCCGGACCAGCCGCACCAACCGGCGCGACGGGAGCCACGGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACGGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacagg                                                                                   <  1:785236/140‑1 (MQ=255)

                                                               ggccgccggacccgccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcga                                                                              <  2:183784/136‑1 (MQ=255)

                                                                gAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCGggagcgccaggcgcgcc                                                                             >  1:7595403/1‑138 (MQ=17)

                                                                 aaGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGCCAGGAGCTACCGGGGAAGCCGGACAAGCCggagcgaccggagcgccc                                                                            >  2:5171744/1‑137 (MQ=255)

                                                                     cggagccgccggaccaACCGGCGCGACTGCAGCCCCGGGAGCTCCCGGGGAAGCGGGACACTCCGGACAAACCGGAGCGACTGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggag                                                                        <  1:4167948/140‑1 (MQ=17)

                                                                     cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggag                                                                        >  1:1114781/1‑140 (MQ=255)

                                                                     cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggag                                                                        >  1:1806194/1‑140 (MQ=255)

                                                                     cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggag                                                                        >  2:651065/1‑140 (MQ=255)

                                                                     cggaccagccggaccaACCGGAGCGACGGGAGCCACCGGAGCTACCGGGGAAGCCGGACCACCCGGACCAACCGGAGCGACAGGAGCGCCAGGAGCTACCGGGGAAGCAGGACCAGCCggagggacaggtgcgacaggag                                                                        >  1:5846723/1‑140 (MQ=14)

                                                                      ggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgaccggagcgacaggagc                                                                       >  2:4828696/1‑140 (MQ=255)

                                                                      ggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagc                                                                       >  2:2041683/1‑140 (MQ=255)

                                                                        accagccggaccaACCGGAGCGCCGGGAGCCACGGGAGCTCCCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcga                                                                     <  2:3878346/140‑1 (MQ=255)

                                                                         ccagccggaccaCCCGGAGCGCCGGGAGCCACGGGAGCCACCGGGGAACCCGGCCCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                                                    <  2:5347672/140‑1 (MQ=17)

                                                                         ccagccggaccaCCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                                                    <  1:659848/140‑1 (MQ=255)

                                                                            gccggacccAGCGTCGGGACGGGAGCCTCGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg                                                                 <  2:6342559/140‑1 (MQ=17)

                                                                            gccggaccaCGGGGAGCGACTGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACTGGAGCGACAGGAGCTACCGTGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgactgg                                                                 <  2:7747705/140‑1 (MQ=17)

                                                                            gccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                                                                          >  2:1480625/1‑83 (MQ=255)

                                                                             ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgaca                                                                                     <  2:1937540/119‑1 (MQ=255)

                                                                             ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAgcgacaggagcgaccggcgcgaccgga                                                                >  1:1258742/1‑140 (MQ=255)

                                                                              cggacctCCCGGAGCGCCGGGAGCCACGGGAGCTACCGGTGAAGCCGGACCAGCCGGACCAACCGGAGCGACTGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGcgacaggagcgacaggagcgacaggag                                                               <  1:5066182/140‑1 (MQ=14)

                                                                                     aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                   <  1:2321658/97‑1 (MQ=255)

                                                                                     aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                   >  2:2321658/1‑97 (MQ=255)

                                                                                     aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGCCAGGAGCGACAGGAGCTACCgg                                                        >  1:515143/1‑140 (MQ=255)

                                                                                      ccGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  1:1832753/140‑1 (MQ=255)

                                                                                      ccGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  1:1608025/140‑1 (MQ=255)

                                                                                          cgCGACGGGACCCCCGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   <  1:2659285/139‑1 (MQ=255)

                                                                                          aGCGCCGGGAGCCACGGGAGCGACCGGGGAAGCCGGACCAGCCGGACCAACCGCAGCCACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   <  1:548471/140‑1 (MQ=255)

                                                                                          aGCGACTGGAGCAACGGGAGCGACCGTGTGAGCCGGAGCATCCGGACCAACCGGAGCGACAGTGTCGACAGGAGATACCGGGGCCGCCGGACCAGCTGGAGCGACAGGAGCGTCAGGAGCGCCAGGACCTACCGGGGAAg                                                   <  2:1678481/140‑1 (MQ=9)

                                                                                          aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   >  1:4351153/1‑140 (MQ=255)

                                                                                          aGCGACAGGAGCCACCGGAGCTACCGGCGAACCCGGACCAGCCGGACCACCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   <  2:5974551/140‑1 (MQ=14)

                                                                                               cGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGTGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa                                              >  2:4689867/1‑140 (MQ=255)

                                                                                               cGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTggc                                              >  2:918721/1‑139 (MQ=255)

                                                                                               cGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa                                              <  1:4713156/140‑1 (MQ=255)

                                                                                               cGGGAGCCACGGGAGCGACCGGGGAAGCCGCCCCAGCCGGACCAACCGGAGCGACAGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa                                              <  1:2509909/140‑1 (MQ=14)

                                                                                                    gCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACCGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGAGCGACCGGAGCTACCGGGGCCGCTGGACCAGc                                         >  2:2953505/1‑140 (MQ=17)

                                                                                                     ccACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGTAGCGACGGGTGCGGCAGGAGCTACCGGGGAAGCTGGACCAGCt                                        >  2:3841069/1‑140 (MQ=255)

                                                                                                     ccACGGGAGCGACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTTCCGGGGAAGCTGGACCAGCt                                        <  1:6082571/140‑1 (MQ=255)

                                                                                                            aGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGa                                 <  2:5222725/140‑1 (MQ=255)

                                                                                                              ctcccGGCGCAGCCGGACCAGCCGGACCAACCCGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGGGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACg                               <  2:5946468/137‑1 (MQ=17)

                                                                                                              cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                                                    >  2:4589746/1‑103 (MQ=255)

                                                                                                              cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa                                              >  2:3471291/1‑125 (MQ=255)

                                                                                                              cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGa                                              <  1:3471291/125‑1 (MQ=255)

                                                                                                                acggggtAAGCCGGACCAGCCGGACCACCCGGAGCCCCAGCAGCCACTGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                             <  1:1029910/137‑1 (MQ=14)

                                                                                                                aCCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                             <  2:6179962/140‑1 (MQ=255)

                                                                                                                aCCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                             <  1:6000153/140‑1 (MQ=255)

                                                                                                                aCCGGCGCACCCGGACCAGCCGCACCAACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                             <  2:354758/140‑1 (MQ=14)

                                                                                                                  cgggcccgccggacccgccggaccaCCCGGAGCGACTGCAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAg                           <  1:681863/133‑1 (MQ=14)

                                                                                                                  cGGGTAAGCGGGACCAGCGGGACCCGCCGGCGCGACAGGAGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAg                           <  2:7688583/140‑1 (MQ=14)

                                                                                                                  cGGGGACGCCGGACCCGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCGACAGGAGCGCCAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAg                           <  1:4106071/140‑1 (MQ=255)

                                                                                                                  cGGGGAAGCCGGACCAGCCGGACCACCCGGCGCGACCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAg                           <  2:1302006/140‑1 (MQ=255)

                                                                                                                  cGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAg                           <  1:4612923/140‑1 (MQ=255)

                                                                                                                  cGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAg                           <  1:4654122/140‑1 (MQ=255)

                                                                                                                  cGGGCAACCCGCACCAGCCGCACCAACCGGAGCCACCGGACCGACAGGAGCTACCGGGGAAGCGGGACACGCCGGAGCGACAGGAGCTACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAg                           <  1:353335/140‑1 (MQ=17)

                                                                                                                      gctcccggaccagccggcccaCCCGGAGCGACCGGCGCCACGGGAGCTACCGGGGAAGCCGGCCCAGCCGGAGCCCCCGGAGCGACTGGAGCGACCGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                       <  1:569229/136‑1 (MQ=11)

                                                                                                                       aaGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgaca                                                                                     <  1:6154350/77‑1 (MQ=255)

                                                                                                                        cgccggcccagccggaccacCCGGAGCTTGAGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCGACAGGAGCGACTGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAG                     <  2:5856571‑M1/139‑1 (MQ=255)

                                                                                                                        agccggaccaccaggaccTCCCGGAGCGAGCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAG                     <  2:5615726‑M1/140‑1 (MQ=255)

                                                                                                                         gccggaccagccggaccaCCCGGAGCCACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGG                    <  1:7443308‑M1/140‑1 (MQ=255)

                                                                                                                         gccggaccagccggaccaACCGGAGCGACAGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGG                    <  1:711752‑M1/140‑1 (MQ=255)

                                                                                                                           cggaccagccggaccaACGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAG                  <  1:7656629‑M1/140‑1 (MQ=255)

                                                                                                                             gaccagccggaccaACCGGAGCGACAGGAGCGACAGGATCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCt                <  2:4368524/140‑1 (MQ=255)

                                                                                                                              accagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTa               >  2:6286162/1‑140 (MQ=255)

                                                                                                                                  gccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                                                    <  2:4470161/83‑1 (MQ=255)

                                                                                                                                   ccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCggg          >  1:1681821/1‑140 (MQ=255)

                                                                                                                                   ccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCGACAGGGGCTACAGGCGCTCGCGGAGACGCAGGACCTCCCCGAGCGCCCCGGGCCACAGGAGGTAGCggg          >  1:5330426/1‑140 (MQ=9)

                                                                                                                                       accaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAg      <  1:4515887/140‑1 (MQ=255)

                                                                                                                                       accaACCGGAGAGAGCGGACCCACAGGAGCTCCCGGCGCACCCGGACCACCCGGACCGCCAGGAGCGACAGGACCTCCAGGAGCTACCGGTGAAGCAGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAg      <  1:3701845/140‑1 (MQ=14)

                                                                                                                                           aCCGGAGCGACAGGAGCGACAGGGGAATCCGGCGAACCCGGACCACCCGGAGCGACAGGAGCGACTGGGGCGCCCGGAGCTGCCGGCGACGCTGGAGCGCCGGGGGCGGCGGGAGCGAGAGGAGCGGCCGGCGacggggg  >  1:7248129/1‑134 (MQ=255)

                                                                                                                                           aCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGTAGCAGCCGGAGAGGCAGGACCAGATGGAGCGACGGGAGCTGCAGGAGCTACGGGAGACGCCgc  >  1:4793429/1‑139 (MQ=17)

AGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGG  >  NZ_CP034943/607274‑607552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`