Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,676 C→G 5.4% T173T (ACC→ACG EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,6760CG 5.4% 758.1 / 25.6 298T173T (ACC→ACGEO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base C (159/119);  new base G (8/10);  total (169/129)
Fisher's exact test for biased strand distribution p-value = 3.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 307 total aligned reads displayed.

ACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGG  >  NZ_CP034943/607539‑607814
                                                                                                                                         |                                                                                                                                          
tccGGCGAAGCCGGTGCACCGGGTGCCTCCGGAGCCACAGGCGCAACTGGCGAACCAGGACCACCCGGAGCCACAGGAGCTCCCGGTGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                                                                          <  1:2058935/139‑1 (MQ=2)
aCCGGGGCAGCCGGACCCGCCGGCGCCACGGGAGCCACCGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                                                                          <  2:515143/140‑1 (MQ=17)
aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACTGGGGCTAAAGGGGAATCCGGACCAGCCGGAGCGACAGGACCAACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGAAGCACCCACCgg                                                                                                                                          >  1:2201335/1‑140 (MQ=17)
aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACCGCAGCGACCGGGGAAGCCGGACCAACCgg                                                                                                                                          >  1:624224/1‑140 (MQ=255)
aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCgg                                                                                                                                          >  2:1860744/1‑140 (MQ=255)
aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCAACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGCAGCGACAGGAGCTCCGGGGGACCCCGGCCCTACCgg                                                                                                                                          >  2:6967596/1‑140 (MQ=14)
         cccGGCCCCGCCGGGCCCCCGGGCGCCCCCGGCCCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                                                                 <  2:2781560/139‑1 (MQ=11)
           cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                                                               <  1:2953505/140‑1 (MQ=255)
                  gCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCAACCGGGGAACCCGGACCACCCGGAGCGCCAGGAGCGACCgg                                                                                                                        >  2:214354/1‑140 (MQ=14)
                   ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCTGACCCGCCCGACCGACCGGAGCTACCGGGGCAGCCGGACCGACCGGAGCGCCAGGAGCGCCCggg                                                                                                                       >  1:7518036/1‑140 (MQ=17)
                   ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                                                                       <  1:6694291/140‑1 (MQ=255)
                   ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                                                                       >  1:5790553/1‑140 (MQ=255)
                       ggCCACGGGAGCCACAGGGGCTACTGGTGACGCCGCACCAGCCGGAGCGACAGCAGCTACCGGGGAAGCCGGTCGATCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCTCCCGGGGacg                                                                                                                   >  2:207577/1‑138 (MQ=14)
                            cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                              >  1:3136927/1‑140 (MQ=255)
                             gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                             >  1:5312208/1‑140 (MQ=255)
                             gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                             <  1:2706624/140‑1 (MQ=255)
                             gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                             >  1:3922388/1‑140 (MQ=255)
                                 gcgaccggggCTACGGGGGAAGCCGGAGCCGCCGGAGCGACAGGTGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                                                         <  2:1163429/134‑1 (MQ=11)
                                 gCCACAGGGGCTCCTGGGGAAGCCGTACCAGCCGGAGCGACAGGAGCTACCGTGGAAGCCGGACCAGCCGGAGCGACAGGAGCCACCGGTGCACCAGGCCCAGCCGCAGCGACCGGAGCAACAGGCGAATCCCGAcatga                                                                                                         >  1:4555594/1‑136 (MQ=2)
                                 gCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAACGGAGCGACCGGAGCAACCGGGGGAGCAGGACCAGc                                                                                                         >  1:3367334/1‑140 (MQ=14)
                                     caGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGa                                                                                                     >  2:3476655/1‑140 (MQ=255)
                                     caGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGa                                                                                                     >  2:1235228/1‑140 (MQ=255)
                                      cgcggCCACCGGCGAAGCCGGACCACCCGGAGCGACAGCAGCTACCGGGGACCCCGGACCACCCGGAGCGACCGGAGCTACCGGGGAAGCCGCACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                                                                    <  2:4426687/137‑1 (MQ=11)
                                         ggCTCCCGGGGAAGCCGGACCAGCCGGCGCGTCAGGTTCTAGCGGGGTCGCCGGTCGAGCCGGTGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa                                                                                                 <  1:7192493/140‑1 (MQ=11)
                                          gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                                                                                            >  1:4346376/1‑96 (MQ=37)
                                          gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                                                                                            <  2:4346603/96‑1 (MQ=37)
                                          gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                <  1:3841069/140‑1 (MQ=40)
                                           cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGGGACAGGGAAAGCAGTACCAGCCCGAGCGACCGGAGCAAACGGGGAATCCCGACCAACCGGAGCGACAGGAGCTACGGGGGGAGCCGGGCGAGCCGGCGGGAcc                                                                                               >  1:3975513/1‑139 (MQ=9)
                                             aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                                                                          >  1:1550330/1‑95 (MQ=37)
                                             aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGCAGCTACAGGAGCGACCGCGGACGCCGGACCAGCCGGAGAGACAgg                                                                                             >  2:7645203/1‑140 (MQ=14)
                                                   gAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAGCCGGAGCGACAGCAGCtcc                                                                                       >  2:7438154/1‑138 (MQ=255)
                                                   gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                                                       >  2:5260997/1‑140 (MQ=255)
                                                    aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa                                                                                                                                         <  2:3858392/89‑1 (MQ=33)
                                                    aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACTGGAGCTACCGGGGAAGACGGACAATCCGGAGCGCCAGCAGCCAca                                                                                      >  2:4614005/1‑139 (MQ=11)
                                                    aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                        >  1:5467149/1‑122 (MQ=38)
                                                    aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                      >  2:1707905/1‑140 (MQ=255)
                                                    aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                      >  2:927265/1‑140 (MQ=255)
                                                    aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAACAGCCGGAGCGACAGGAGCTACCGGCGACTCCGGAACAACCGGAGCGACAGTAGCTCCCGGGGCAGCCGGAGCAGACAGAGCGACAGGaacacac                                                                                      >  2:1118453/1‑134 (MQ=14)
                                                      gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                   >  2:6723553/1‑109 (MQ=35)
                                                      gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                    >  2:4115359/1‑140 (MQ=255)
                                                      gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACCGGAGCTACCgg                                                                                    >  2:4254078/1‑140 (MQ=255)
                                                      cccGGACCACCCGGAGCCCCAGGAGCTCCCGGCGACCCCGGAGCACCCGGAGCGACAGGAGCACCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                    <  1:4376731/139‑1 (MQ=14)
                                                       cgggACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                   <  1:1444590/138‑1 (MQ=255)
                                                       ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                   <  1:4855992/140‑1 (MQ=255)
                                                        cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                                                                  <  1:127254/140‑1 (MQ=255)
                                                           acctcccGGAGCGACCGGAGCTTCCGGGGACGCCGGAGCACCAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                               <  1:5078321/135‑1 (MQ=14)
                                                            ccACCCGGAGATACAGGAGCTCCCGGGGACCCCGGACCAGCCGGAGCCCCAGGAGCTACCGGAGAAGCCGGACCAACCGGAGCGACAGGCGCTACCGGGGAAGCGGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc                                                                              <  1:2532430/140‑1 (MQ=14)
                                                               gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                      <  1:6732857/113‑1 (MQ=39)
                                                               gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                                                                           >  2:684514/1‑140 (MQ=255)
                                                                    aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCaa                                                                      >  1:6603823/1‑140 (MQ=255)
                                                                         cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGa                                                                 >  2:3220454/1‑140 (MQ=255)
                                                                               cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACg                                                           <  1:1742167/140‑1 (MQ=40)
                                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCgg                                                                                                                        >  2:7088598/1‑77 (MQ=39)
                                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCgg                                                                                                                        >  2:145833/1‑77 (MQ=39)
                                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCgg                                                                                                                        >  2:4069413/1‑77 (MQ=39)
                                                                                   cGGGGCAGCCGGACCACCCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCGGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAg                                                       <  1:2008807/140‑1 (MQ=255)
                                                                                       gcaGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                   <  1:3647261/138‑1 (MQ=255)
                                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGTAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                   <  1:2585729/140‑1 (MQ=255)
                                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                                                                    <  1:4092974/91‑1 (MQ=37)
                                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                   <  1:7415905/140‑1 (MQ=255)
                                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                   <  2:3922388/140‑1 (MQ=255)
                                                                                       gAACCCGGACCACCCGGAGCAACAGGACCTCCCGGGGCCGCCGGACCAACCGGAGCGACAGGAGCTCCCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                   <  1:1345719/140‑1 (MQ=14)
                                                                                       ccccccGGACCCTCCGCCGCGACAGCCGCTACCGCGGCAGCCGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                   <  2:6738991/136‑1 (MQ=14)
                                                                                        aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                  <  1:5901535/140‑1 (MQ=255)
                                                                                          gCCGGACCAGCCGGATCGACAGGAGCTACGGGGGTAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCGGTACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                <  2:4396584/140‑1 (MQ=14)
                                                                                          gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCg                                                                                                                         >  2:305948/1‑67 (MQ=35)
                                                                                          gCCGGACCACCAGGATCTACAGGAGCTTGGGGGTAAGCCGGACCTACCGGAGCTACAGGAGATACGGGGTAAGCGGGACCAGCCGTATCGAGAGGAGATACCGGGGAAGCCGGACCAACCGGAGCGACGTGAGCCACAgg                                                <  2:596025/140‑1 (MQ=9)
                                                                                          cccGGACCTCCCGGAGCCACCGGAGCTACCGGCGAAGCCGGACCATCCGGAGCTACCGGAGCTACCGGGGAAGCCGGACCAGCAGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGCAGCGACGGGAGCCACCgg                                                <  2:5355146/139‑1 (MQ=11)
                                                                                               accccacGGAGCTCCCGGAGCCACCGGCGCCACCGGACCAACCGGAGCCCCAGGAGCTCCCGGCCCAGCCGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa                                           <  2:896402/134‑1 (MQ=9)
                                                                                               aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                                  >  2:5820955/1‑69 (MQ=35)
                                                                                               aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                                  >  2:6382787/1‑69 (MQ=35)
                                                                                               aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGcga                                           >  2:1643342/1‑138 (MQ=255)
                                                                                               aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa                                           >  2:5196651/1‑140 (MQ=255)
                                                                                               aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACGGGGGAACCCGGACCATCCGGAGCGGGGGGATCCGCAggcggga                                           >  2:5430110/1‑135 (MQ=11)
                                                                                                    caggAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                   >  2:6127079/3‑127 (MQ=255)
                                                                                                              aGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                            <  2:6680854/140‑1 (MQ=255)
                                                                                                                   cTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGcc                       <  1:684514/140‑1 (MQ=255)
                                                                                                                     cccGGGGAAGCCGGACCTACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                     <  2:1286250/139‑1 (MQ=255)
                                                                                                                     aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                          <  1:962778/87‑1 (MQ=38)
                                                                                                                          ggAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACGTCGGACCAGCCGGAGCGa                >  1:926471/1‑140 (MQ=255)
                                                                                                                          ccaccccGGACCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGa                <  1:1024975/135‑1 (MQ=17)
                                                                                                                           gcaGCCGGAGCAACGGGAGCGACAGGAGCTACCGCCGAAGCCGCACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc               <  1:5948355/138‑1 (MQ=21)
                                                                                                                           gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc               <  1:3476655/140‑1 (MQ=255)
                                                                                                                           gAAGCCGGACCAACCGGAGCGACAGGAGCCACCGGGGAAGCCGGACCAGGCGGAGCGCCAGGAGCGACCGGCGAAGCCGGACCACCCGGAGCGACGGGCGCGACAGGAGCACCGGGCGCCGGCGGACCAGCCGCAGCggc               >  2:371827/1‑138 (MQ=14)
                                                                                                                            aaGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                         >  2:6625711/1‑97 (MQ=37)
                                                                                                                             aaccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAg             >  2:2718191/3‑140 (MQ=14)
                                                                                                                              gCCGGACCAACCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGCCCGGGGACGCGGGACCTACCGGTGCGACGGGAGCCAAAGCTCCAACGGGGGAAGGCGGAACAGGCGCAGAGACatg            >  2:2613489/1‑138 (MQ=2)
                                                                                                                              gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg            >  2:3635558/1‑140 (MQ=255)
                                                                                                                              gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg            >  1:7022677/1‑140 (MQ=255)
                                                                                                                              gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg            >  1:5237475/1‑140 (MQ=255)
                                                                                                                              gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg            >  1:1494821/1‑140 (MQ=255)
                                                                                                                              gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGAAGACGGACCAGCCGGAGCGAccgc            >  2:5520921/1‑137 (MQ=255)
                                                                                                                              accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg            <  1:4423127/139‑1 (MQ=14)
                                                                                                                              accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg            >  2:4902357/2‑140 (MQ=14)
                                                                                                                               ccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGCCGTCGGACCAGCCGGAGCGTCAgca           >  2:1981024/1‑138 (MQ=14)
                                                                                                                                   aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa       >  2:7300613/1‑140 (MQ=255)
                                                                                                                                   aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa       >  2:1413417/1‑140 (MQ=255)
                                                                                                                                   aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa       >  2:4812020/1‑140 (MQ=255)
                                                                                                                                   aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCTGCCGGAGCGACAGGAGCTa       >  2:1545113/1‑140 (MQ=255)
                                                                                                                                      aaCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCg    >  2:7233493/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                          
ACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGG  >  NZ_CP034943/607539‑607814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: