Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 112,636 G→T 6.0% R4I (AGA→ATA)  cysE → serine O‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943112,6360GT 6.0% 2118.2 / 29.3 768R4I (AGA→ATA) cysEserine O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (183/514);  new base T (69/2);  total (252/516)
Fisher's exact test for biased strand distribution p-value = 4.38e-34
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 771 total aligned reads displayed.

GGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAAACGAAAATT  >  NZ_CP034943/112497‑112774
                                                                                                                                            |                                                                                                                                          
 gagaGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGa                                                                                                                                           <  1:7435922/140‑1 (MQ=255)
 gagaGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGACGAAATCAAATAGAGAAGGGGGGGGAAGCATGTGTTTTTTAGa                                                                                                                                           <  2:6101026/140‑1 (MQ=255)
    agCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTGGGTGTAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTATAATg                                                                                                                                        >  1:5562933/1‑140 (MQ=255)
           gTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaag                                                                                                                                 <  1:6608930/140‑1 (MQ=255)
           gTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaag                                                                                                                                 <  2:6598322/140‑1 (MQ=255)
           gTCGGCGTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGAGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaag                                                                                                                                 <  2:3265119/140‑1 (MQ=255)
           ctctcCTTTACAAACATAGTCGAACCGCGCGTATTAAGCGTATATGTCATGTTTTCATGCAGAGGCGCTATTTTTATTCGGTGAAGAGGAAAACAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaag                                                                                                                                 <  2:4063010/139‑1 (MQ=255)
           ctCTGCTTTTCAAACAGAGTCGAACCGCGCGGTTTAAGAGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaag                                                                                                                                 <  2:4579365/139‑1 (MQ=255)
             cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                                                                               <  2:4806218/140‑1 (MQ=255)
               tcTTTTCAAACAGAGTGGAACCGCGCTGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTTTTTAAGAGTAAATCAAATAGAGAATTTGGGGTAAGCATTTTTTTTTTAGAATGCTCAAagaaga                                                                                                                             <  1:692850/139‑1 (MQ=255)
               gCTTTTCAACCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGAGAAGAGTAAATCAAATAGAGAAGTGGGGGTAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                                                             <  1:7633921/140‑1 (MQ=255)
               gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                                                             <  2:2712284/140‑1 (MQ=255)
               gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTAATGCAGAGGCGCTTTTTTTAGTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                                                                             <  1:823246/140‑1 (MQ=255)
                agacagaaaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGCAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGAc                                                                                                                            <  1:3709268/134‑1 (MQ=255)
                 ttttCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGACGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACa                                                                                                                           <  1:3313546/140‑1 (MQ=255)
                  tttCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACAt                                                                                                                          <  1:1827470/140‑1 (MQ=255)
                     cAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTTTTTTTTTTGGGGGAGAGGGAATCAAATATAGTAGTTGGGGGGAGCAGGGGTTTTTTATAATTCTCAAAGAAAACaattt                                                                                                                       >  1:4670348/1‑136 (MQ=255)
                       aaCTGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAACTAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                                                     <  2:3819037/140‑1 (MQ=255)
                       aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                                                     <  2:7679113/140‑1 (MQ=255)
                       aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                                                     <  1:3502674/140‑1 (MQ=255)
                       aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                                                     <  1:515736/140‑1 (MQ=255)
                       aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTGATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                                                                     <  1:985514/140‑1 (MQ=255)
                         cAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACa                                                                                                                   <  1:1920477/140‑1 (MQ=255)
                            agTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAgtg                                                                                                                <  1:5707971/140‑1 (MQ=255)
                              tGGAACCGCGCGGTTTACGCGTCTCTGTCATGTTTTCAGGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATCGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGtt                                                                                                              <  2:5025433/140‑1 (MQ=255)
                              tGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGtt                                                                                                              <  2:3169162/140‑1 (MQ=255)
                                    cgcgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAACTAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCa                                                                                                        <  2:1983625/140‑1 (MQ=255)
                                      cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCCGAGGCGCTTTTTTTATTGGGTGGAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTATAATGCTCAAAGAAGACCTTTTTAAACTGTTCGATCaat                                                                                                      >  2:2455862/1‑139 (MQ=255)
                                      cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGCGGCGCTTTTTTTATTTGGGGTAGACGAAATGAAATATTGAATGGGGGGGACGCCTGTGTTTTTTTGAATTCTGTAAGAAACAATTGATACCGTGTTAGGTCAAg                                                                                                      >  1:100097/1‑140 (MQ=255)
                                      cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTGGGTGAAGAGGAAATAAAATATAGAAGTGGGGGGAAGCAAGTGTTTTTTATAATTATAAAATAAGACAATGATACAGTGTTCGagaaaa                                                                                                      >  1:3828336/1‑135 (MQ=255)
                                      cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGAAGAGGAAAACAAAAAGAGAAGTGGGGGGAACCCAGTGTTTTTTATTATTCTCAAATAAAAAATTGATAAAGTGTTCGATAAAg                                                                                                      >  1:7504877/1‑140 (MQ=255)
                                      cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGTGTGAAGAGGAAATAAAATAGAGAAGTGGGGGGAAGCAAGTGTTTTTTAGAATGGTCAAAGAAGACATTGATACAGTGTTCGATAAAg                                                                                                      >  2:4929269/1‑140 (MQ=255)
                                      cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGTGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATTCTCAAAGAAGACATTGATACAGTGTTAGATCAAg                                                                                                      >  2:5136207/1‑140 (MQ=255)
                                      cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGTAATCAAATAGATAAGTGGGGGGAAGCATGTGTTTTTTAGAATTCTAAAAGAAGACATTGATAAAGTGTTAGATCAAg                                                                                                      >  2:2077532/1‑140 (MQ=255)
                                      cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATTCTCAAAGAAGACAATTATAAATTGTTCGATaaaa                                                                                                      >  2:5827986/1‑139 (MQ=255)
                                      cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGGTCGATAAAg                                                                                                      >  2:6736530/1‑140 (MQ=255)
                                      cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGGAAGAGGAAATCAAATAGAGAAGGGGGGGGAAGGATGTGTTTTTTAGAATGCTCAAAGAAGACAATGATACCGTGTTCGATCAAg                                                                                                      >  2:2805436/1‑140 (MQ=255)
                                           tttAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGGGAAGAGGAAAACAAATAGAGAACTGGGGGGAAGCCTTTGTTTTTTAGAATGCTCAAATAACACAATTAAACAGGGTTCGATCAATATAca                                                                                                 >  1:6111689/1‑140 (MQ=255)
                                                     tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTTGGGGTATACGCAAAACAAAAGAGCAGGGGGGGGGAGCATGTGTTTTTTTTGATGCGGAAAAAAGACAATGAGAAACTGTTAGGTAAAAATCCACCAGCaacca                                                                                       >  1:2997412/1‑137 (MQ=255)
                                                     tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTTGGGGGAGAGGGAAAAAAAAAGAGACATGGGGGGGCGCATGTGTTTTTTTTAAATCTCAAAAAAAACAATGATAAAGTGTTGGATAAAAAAACAGCAGCaagaa                                                                                       >  1:2053176/1‑140 (MQ=255)
                                                     tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTATAATTCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaa                                                                                       >  2:7167810/1‑140 (MQ=255)
                                                     tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTTATACAGTGTTCGATCAAGATCCAGCAGCaagaa                                                                                       >  2:2523587/1‑140 (MQ=255)
                                                     tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAACCAGGTTTTTTTTATAATGCTCAAAGAAGACATTGATAAAGTGTTCGATCAAGATCAAGCAGCaagaa                                                                                       >  2:6254236/1‑140 (MQ=255)
                                                     tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGGCAGAGGAAATCAAATAGAGAAGGGGGGGGTAGCATGTGTTTTTTAGCATGCTCAAAGAAGACAGTTATGCAGTGTTAGATAACGATCCAGCAGCaagaa                                                                                       >  2:4146756/1‑140 (MQ=255)
                                                     tctGTAATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAATAGGAAATCCATTAGATAAGTGGGGGTAACCAGGTGTTTTTTAGAATTCTAAATGTAGACATTTATACAGTGTTCGAGGAAGATACCGCAGCtagta                                                                                       >  2:1319332/1‑138 (MQ=255)
                                                     tcggTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAATTAGAGAAGTTGGGGGAAGCATGGGTTTTTTAGAATGCTCAAAGAAGACCTTGATACCCTGTTCGATCAAGATCCAGCAGCaagag                                                                                       >  2:4310298/4‑139 (MQ=255)
                                                      ctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGCTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaag                                                                                      <  2:928553/140‑1 (MQ=255)
                                                      ctGTCATGTGTTCATGCAGAGGCGCTTTTTTGATTGGGTGAAGAGGAACTCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaag                                                                                      <  1:7075693/140‑1 (MQ=255)
                                                       tGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGTATAGGAAAACAAATATAGAACTGGGGGGCAGCAAGTGTTTTTTTTAATTCTCAAAAAAGACAATGATAAAGTGTTAGATAAAGATACACCAGCAAAAAGt                                                                                     >  1:2606221/1‑140 (MQ=255)
                                                           aTGTTTTCATGCCGAGGCGCTTTTTTTATTGGGGGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTAtt                                                                                 >  2:1836040/1‑140 (MQ=255)
                                                              ttttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGTAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTTTTACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTg                                                                              >  2:3558997/1‑140 (MQ=255)
                                                                ttCATGCAGAGGCGCTTTTTTTATTGGGTTAAGAGGAAATCAAATATAGAAGTGGGGGGAACCATGTTTTTTTTAGAATGATCAAAGAAGACAATTATACAGTGTTAGATAAATATCCAGCAGCAAGAAGTTATTTTGaa                                                                            >  2:1238332/1‑140 (MQ=255)
                                                                ttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGACGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGaa                                                                            <  1:2433419/140‑1 (MQ=255)
                                                                  tccggagagGGGCATTTTTTTTTGGGGGCAGCCGAAAACAAATTCCCCAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGt                                                                          <  1:906701/135‑1 (MQ=255)
                                                                  cATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGt                                                                          <  1:5912861/140‑1 (MQ=255)
                                                                              cTTTTTTTATTTGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTAAAAGAACAAAATTATACACTTTTCGAACAAAATACAGCATCAAAAAGTTATTTTTAATTGTTTTTAAATTa                                                              >  2:6914540/1‑140 (MQ=255)
                                                                              cTTTTTTTATTTGGTGAAGAGGAAAACAAATAGAGACGGGGGGGGAACGAGGTGTTTTTTAGAATGCTCAAATATGACATTTATACATGTTTAGATCAATATCAAACACCAAGAATGTAATTTGTAGTGATTTTAAGTTa                                                              >  2:7279051/1‑140 (MQ=255)
                                                                              cTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAACTGGGGGGCAGCATGTGTTTTTTAGAATTCTCAAAGAACACAATGATACAGTGTTCGATCAAGATCCACCACCAAGAAGTTATTTTGAAGTTATTTTAAATTa                                                              >  2:302194/1‑140 (MQ=255)
                                                                                gtttttATGGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTGTAGAATGCTCAAAGCAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTAtt                                                            <  1:4236213/139‑1 (MQ=255)
                                                                                  ttttATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTcc                                                          <  1:1547460/140‑1 (MQ=255)
                                                                                        tcgctGAAGAGCAAATCAAATACAGAAGTGGGGCGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGAATTTAACTTATTCCGGATTa                                                    <  2:7517857/136‑1 (MQ=255)
                                                                                        tGGGTGCAGAGGAAATCAAATAGAGACGGGGGGGGAAGCATGTGTTTTTGAGAATGCTCAAAGACGACATTGATACAGTGTTCGATCAAGAGCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa                                                    <  1:3206608/140‑1 (MQ=255)
                                                                                        tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa                                                    <  2:6348808/140‑1 (MQ=255)
                                                                                        tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa                                                    <  2:4464410/140‑1 (MQ=255)
                                                                                        agggTGAAGACGAAATCAAATAGAGACGTGGGGGGAAGCATGTTTTTTTTAGAATGCTCAAAGAATACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa                                                    <  2:7418450/139‑1 (MQ=255)
                                                                                          ggTGAAGAGGAAATCACATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                  <  1:2109964/140‑1 (MQ=255)
                                                                                          ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTGACTTATTCCGGTTTACa                                                  <  1:4994649/140‑1 (MQ=255)
                                                                                          ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                  <  1:1279560/140‑1 (MQ=255)
                                                                                          ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                  <  2:1716612/140‑1 (MQ=255)
                                                                                          ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                  <  1:4540869/140‑1 (MQ=255)
                                                                                          ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                  <  2:2944423/140‑1 (MQ=255)
                                                                                          ggTGAAGAGGAAATCAAATAGAGAAGGGGGGGGAAGCAGGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                  <  1:2828974/140‑1 (MQ=255)
                                                                                            tGAAGAGGAAATCACATAGAGAAGTGGGGGGACTCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATg                                                <  1:4043304/140‑1 (MQ=255)
                                                                                            tGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATg                                                <  2:1162501/140‑1 (MQ=255)
                                                                                            tGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATg                                                <  2:5388739/140‑1 (MQ=255)
                                                                                            tGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATg                                                >  2:367066/1‑140 (MQ=255)
                                                                                                      aaTCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATggg                                       >  1:1109523/1‑140 (MQ=255)
                                                                                                       aTCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaag                                                                                      >  2:4671298/1‑92 (MQ=255)
                                                                                                       aTCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                  >  2:5515405/1‑128 (MQ=255)
                                                                                                        tCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGgcg                                     <  2:141722/140‑1 (MQ=255)
                                                                                                          aaaTAGAGAAGTGTGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCa                                   <  1:3461498/140‑1 (MQ=255)
                                                                                                          aaaTAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCa                                   <  1:1382697/140‑1 (MQ=255)
                                                                                                          aaaTAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCa                                   <  1:5988714/140‑1 (MQ=255)
                                                                                                               gagaAGTGGGGGTAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGa                              <  1:6840138/140‑1 (MQ=255)
                                                                                                               gagaAGTGGGGGGACGCATGTGTTTTTTAGAATGCTCAAAGAAGCCATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGa                              <  1:5468878/140‑1 (MQ=255)
                                                                                                                  aaGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTg                           <  2:2184449/140‑1 (MQ=255)
                                                                                                                     tGGGGGGAATCATGTGTTTATTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGcac                        <  1:7208897/140‑1 (MQ=255)
                                                                                                                     tGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGcac                        <  1:6270183/140‑1 (MQ=255)
                                                                                                                     tGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGcac                        <  2:7104835/140‑1 (MQ=255)
                                                                                                                     tGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGcac                        <  1:4366785/140‑1 (MQ=255)
                                                                                                                     gggggggAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGcac                        <  1:6389353/139‑1 (MQ=255)
                                                                                                                          ggAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCt                   <  1:4797029/140‑1 (MQ=255)
                                                                                                                          ggAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCt                   <  1:1706310/140‑1 (MQ=255)
                                                                                                                          ggAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGGTATTTTGAAGTGATTTGAACTTATTCCGGTTTCCATGCTATATGGGCGCATCGGATTGCACATGCt                   <  1:2312186/140‑1 (MQ=255)
                                                                                                                               cATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTtata              >  1:3599746/1‑140 (MQ=255)
                                                                                                                               cATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTtata              >  2:2375530/1‑140 (MQ=255)
                                                                                                                               cATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGATATATGGGCGCATCGGATTGCACATGCTTtcta              >  2:5980609/1‑140 (MQ=255)
                                                                                                                                 tgtgTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTtatata            >  1:4875312/1‑140 (MQ=255)
                                                                                                                                   tgtTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATGTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATaaa          >  2:3430920/1‑140 (MQ=255)
                                                                                                                                           aGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAAACGAAAAtt  <  2:5642382/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
GGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAAACGAAAATT  >  NZ_CP034943/112497‑112774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: