BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,694	C→T	16.5%	T179T (ACC→ACT)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,694	0	C	T	16.5%	671.7 / 122.6	332	T179T (ACC→ACT)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base C (153/116); new base T (43/9); total (202/130)
Fisher's exact test for biased strand distribution p-value = 3.37e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01

Only 100 of 344 total aligned reads displayed.

AGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGA  >  NZ_CP034943/607556‑607832

  ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCTGACCCGCCCGACCGACCGGAGCTACCGGGGCAGCCGGACCGACCGGAGCGCCAGGAGCGCCCggg                                                                                                                                         >  1:7518036/1‑140 (MQ=17)

  ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                                                                                         >  1:5790553/1‑140 (MQ=255)

  ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                                                                                         <  1:6694291/140‑1 (MQ=255)

            gggATCCACAGGCGCTACTGGGTCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                                               <  2:6811156/140‑1 (MQ=11)

            gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCAGGACCACCCGGAGCGCCCGGAGCTACCGGGGAAGCCGGAc                                                                                                                               >  2:67128/1‑140 (MQ=255)

                gcgaccggggCTACGGGGGAAGCCGGAGCCGCCGGAGCGACAGGTGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                                                                           <  2:1163429/134‑1 (MQ=11)

                gCCACAGGGGCTCCTGGGGAAGCCGTACCAGCCGGAGCGACAGGAGCTACCGTGGAAGCCGGACCAGCCGGAGCGACAGGAGCCACCGGTGCACCAGGCCCAGCCGCAGCGACCGGAGCAACAGGCGAATCCCGAcatga                                                                                                                           >  1:4555594/1‑136 (MQ=2)

                gCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAACGGAGCGACCGGAGCAACCGGGGGAGCAGGACCAGc                                                                                                                           >  1:3367334/1‑140 (MQ=14)

                     cgcggCCACCGGCGAAGCCGGACCACCCGGAGCGACAGCAGCTACCGGGGACCCCGGACCACCCGGAGCGACCGGAGCTACCGGGGAAGCCGCACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                                                                                      <  2:4426687/137‑1 (MQ=11)

                        ggCTCCCGGGGAAGCCGGACCAGCCGGCGCGTCAGGTTCTAGCGGGGTCGCCGGTCGAGCCGGTGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa                                                                                                                   <  1:7192493/140‑1 (MQ=11)

                         gctccgggggAAGCGGGCGCAGCGTGAGCGAGAGGAGCTCCAGGAGAACCCGGACCACCCGGACCAACAGGACCTACGGGAGTCCCGGGAGCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                  <  1:2623752/134‑1 (MQ=2)

                         gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                  <  2:6972329/140‑1 (MQ=40)

                         gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                  <  1:3841069/140‑1 (MQ=40)

                          cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGGGACAGGGAAAGCAGTACCAGCCCGAGCGACCGGAGCAAACGGGGAATCCCGACCAACCGGAGCGACAGGAGCTACGGGGGGAGCCGGGCGAGCCGGCGGGAcc                                                                                                                 >  1:3975513/1‑139 (MQ=9)

                          cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACa                                                                                                                 >  2:4855992/1‑140 (MQ=40)

                          cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACa                                                                                                                 >  2:1444590/1‑140 (MQ=40)

                          cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGGAGCCGGAGCAGCCGGAGCGGCa                                                                                                                 >  2:4396241/1‑140 (MQ=14)

                            aCTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGGGAAGCCGCACACGGCGGAGCGACAGGAGCTACCGGGGCTGCCGGGGCGGCCGCGGCGACggg                                                                                                               >  2:1958747/1‑140 (MQ=11)

                            aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                  <  1:1451618/137‑1 (MQ=40)

                                gggACGCCGGACCACCCGGAGCGACAGGAGCTACCGGCGCAGCCGGACCCGCCGGAGCGACAGGAGCTCACGGCGCCGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCt                                                                                                           <  1:2196841/140‑1 (MQ=14)

                                gcgAAGCCGGAGCACCCGGAGCTACAGGAGCTACCGGGGAACCCGGACCACCCGGAGCTACAGGAGCAACCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCt                                                                                                           <  1:2536259/138‑1 (MQ=11)

                                 ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                          >  2:7415905/1‑140 (MQ=255)

                                  gAAGCCGGTCCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCTGCCGGAGCGACAGGAGGTAGGGGGGGAGCCGGACCAAGCGGAGCGCCAGGTGCTCCCGGGGCAGCCGGGCGAGAGGGAGCGAGAGGAGCTAc                                                                                                         >  2:3632714/1‑140 (MQ=14)

                                   aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                                          >  1:5467149/1‑122 (MQ=38)

                                   aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                                        >  2:927265/1‑140 (MQ=255)

                                   aaGCCGGACCAACCGGAGCGACAGGAGCTACCCGGGAATCCGGACCAGCCGGAGCCACAGGAGCTACCGGGGAAGCCCGCCCAACCGGAGCGACCGGAGCAACCGGGGAAGCCGGAGCTGCCGGAGCGCCAGGAGCTccc                                                                                                        >  2:2073871/1‑140 (MQ=14)

                                     gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCAACCGGACCGACCGGAGCGACAGGGGATACCGGCCCAGCCGGAGCAACCGGAGCGACCgg                                                                                                      >  2:21023/1‑140 (MQ=14)

                                       cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGCAGCGCCCGGAGCAACAggcg                                                                                                    >  2:3082843/1‑138 (MQ=11)

                                          acctcccGGAGCGACCGGAGCTTCCGGGGACGCCGGAGCACCAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                 <  1:5078321/135‑1 (MQ=14)

                                              gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                                                                                             >  1:1286269/1‑140 (MQ=255)

                                              gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                        >  2:6732857/1‑113 (MQ=39)

                                              gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                        <  1:6732857/113‑1 (MQ=39)

                                                                aCCGGGGACGCCGTCCCAGCCGGAGCTACAGGCGCTACCGGTGAAGCCGGACCAACGGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                           <  2:2293391/140‑1 (MQ=14)

                                                                aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCgg                                                                                                                                          <  1:4069413/77‑1 (MQ=39)

                                                                aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                           >  2:4212782/1‑140 (MQ=255)

                                                                aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCAACCGGGGAAGCAGGAACAGCCGGAGCGGCCTGAGCTCCACGCGAAGCGGGTCCAACCGGAGCGACcgg                                                                           >  2:3806238/1‑140 (MQ=14)

                                                                  cGGTGAAGCCGGACCAGCCGGAGCGCCAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAg                                                                         <  1:6901642/140‑1 (MQ=255)

                                                                      gccgCCGGACCAGCCGGAGCGACAGGCGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                     <  2:6522067/137‑1 (MQ=255)

                                                                      gcatccGGACCAGCCGGAGATACTGGAGCCACCGGCGCACCCGGAGCAACCGGAGCTCCAGGAGCACCCGGCGCAGCCGGACCCACCGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                     <  1:2023337/136‑1 (MQ=2)

                                                                      gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                     <  2:5312208/140‑1 (MQ=255)

                                                                      gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                     <  1:7415905/140‑1 (MQ=255)

                                                                      gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                     <  2:3922388/140‑1 (MQ=255)

                                                                      gAAGCCGGACCACCCGCAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGCAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCCGGGAAGCCGGACCACCCGGAGCGACGGGAGCcac                                                                     <  1:207577/140‑1 (MQ=14)

                                                                      caacccGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                     <  2:4804837/136‑1 (MQ=255)

                                                                       aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                    <  1:5901535/140‑1 (MQ=255)

                                                                         gCCGGACGATCGGGAGCGACAGGTGCTACGGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                  <  2:7150866/140‑1 (MQ=14)

                                                                         cccccaccaccCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGTGCGACAGGCGCTACGGGGTACGCATGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                  <  1:3269942/135‑1 (MQ=14)

                                                                           cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACGGGCGCCGCCGGCACAGCCGGAGCGCCGGGAGcccacgccg                                                                >  2:5260600/1‑133 (MQ=11)

                                                                            ggACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGc                                                               <  1:2759902/140‑1 (MQ=255)

                                                                              accccacGGAGCTCCCGGAGCCACCGGCGCCACCGGACCAACCGGAGCCCCAGGAGCTCCCGGCCCAGCCGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa                                                             <  2:896402/134‑1 (MQ=9)

                                                                              aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                                                    >  2:6657940/1‑69 (MQ=35)

                                                                              aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                                                    >  2:5820955/1‑69 (MQ=35)

                                                                                                    cccGGGGAAGCCGGACCTACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                       <  2:1286250/139‑1 (MQ=255)

                                                                                                    aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                 >  2:273360/1‑82 (MQ=38)

                                                                                                       ggggAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGc                                    >  1:4921171/1‑140 (MQ=255)

                                                                                                         ccaccccGGACCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGa                                  <  1:1024975/135‑1 (MQ=17)

                                                                                                          gcaGCCGGAGCAACGGGAGCGACAGGAGCTACCGCCGAAGCCGCACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                 <  1:5948355/138‑1 (MQ=21)

                                                                                                          gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGACCCACAGGGGCTACTGGGGACGTCTGACCAGCCGGAGCGAc                                 >  1:4307820/1‑140 (MQ=255)

                                                                                                          gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGCCGGGAGCCACAGGGGCTACGGGGGACGTCGGACCAGCCGCAGCGAc                                 >  2:3355844/1‑140 (MQ=255)

                                                                                                          gAAGCCGGACCAACCGGAGCGACAGGAGCCACCGGGGAAGCCGGACCAGGCGGAGCGCCAGGAGCGACCGGCGAAGCCGGACCACCCGGAGCGACGGGCGCGACAGGAGCACCGGGCGCCGGCGGACCAGCCGCAGCggc                                 >  2:371827/1‑138 (MQ=14)

                                                                                                           aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCCACAGGGGCAACCGGCGAAGGCGCACCAGCCGGAGCGACa                                >  2:6060580/1‑140 (MQ=14)

                                                                                                            aaccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAg                               >  2:7284153/3‑140 (MQ=14)

                                                                                                            aaccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAg                               >  2:1701759/3‑140 (MQ=14)

                                                                                                             gCCGGACCAACCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGCCCGGGGACGCGGGACCTACCGGTGCGACGGGAGCCAAAGCTCCAACGGGGGAAGGCGGAACAGGCGCAGAGACatg                              >  2:2613489/1‑138 (MQ=2)

                                                                                                             gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                              >  1:7022677/1‑140 (MQ=255)

                                                                                                             gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                              <  2:1494821/140‑1 (MQ=255)

                                                                                                             gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                              >  1:1494821/1‑140 (MQ=255)

                                                                                                             gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGAAGACGGACCAGCCGGAGCGAccgc                              >  2:5520921/1‑137 (MQ=255)

                                                                                                             gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAGCCGGAGCGACAGGATCTACCGGGGAACCCGGACAAACAGAAGAGACGGGAGCCACAGGGGCGACGGGGGACGTCGGACCAGCCGGAGCGACAgg                              >  1:3561517/1‑140 (MQ=18)

                                                                                                             accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGTGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                              >  2:5171479/2‑140 (MQ=14)

                                                                                                             accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                              <  1:4423127/139‑1 (MQ=14)

                                                                                                                 gACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCt                          <  1:5260997/140‑1 (MQ=255)

                                                                                                                  aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                                                >  1:302449/1‑37 (MQ=40)

                                                                                                                           aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                <  1:975370/140‑1 (MQ=255)

                                                                                                                            gccaCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc               >  2:535828/4‑140 (MQ=18)

                                                                                                                                cAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa           >  1:4727269/1‑140 (MQ=255)

                                                                                                                                cAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa           >  1:4126231/1‑140 (MQ=255)

                                                                                                                                cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                <  1:1194869/135‑1 (MQ=255)

                                                                                                                                cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                >  2:1194869/1‑135 (MQ=255)

                                                                                                                                cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa           >  2:7571664/1‑140 (MQ=255)

                                                                                                                                 aGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACAGGGGACGTCGGACCAGCCGGATCGCAAGCAGCTACCGGGGAAGACGGAc          >  2:507295/1‑140 (MQ=17)

                                                                                                                                     gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc      >  1:530986/1‑140 (MQ=255)

                                                                                                                                     gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc      >  1:538419/1‑140 (MQ=255)

                                                                                                                                     gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc      >  1:6354850/1‑140 (MQ=255)

                                                                                                                                     gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc      >  2:4093816/1‑140 (MQ=255)

                                                                                                                                     gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc      >  1:1688013/1‑140 (MQ=255)

                                                                                                                                     gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc      <  2:1047687/140‑1 (MQ=255)

                                                                                                                                     gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc      >  1:939162/1‑140 (MQ=255)

                                                                                                                                     gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACAAGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCTGACCAAc      >  1:644168/1‑140 (MQ=18)

                                                                                                                                     gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAAc      >  2:617335/1‑140 (MQ=255)

                                                                                                                                     gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACAGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACACGGACCAAc      >  2:1633087/1‑140 (MQ=255)

                                                                                                                                     gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAAc      >  2:4007503/1‑140 (MQ=255)

                                                                                                                                      cTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc     >  2:3170777/1‑140 (MQ=255)

                                                                                                                                       tACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCG    >  1:3688657‑M1/1‑140 (MQ=255)

                                                                                                                                        aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                     >  2:4593847/1‑74 (MQ=39)

                                                                                                                                         ccGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGt                                                   <  1:182496/91‑1 (MQ=255)

                                                                                                                                         ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGt                                                   >  2:182496/1‑91 (MQ=255)

                                                                                                                                         ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGA  <  1:922210‑M1/140‑1 (MQ=255)

                                                                                                                                         ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGA  >  2:6275967‑M1/1‑140 (MQ=255)

                                                                                                                                         ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGA  >  2:54518‑M1/1‑140 (MQ=255)

AGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGA  >  NZ_CP034943/607556‑607832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`