BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,700	A→C	22.3%	E181D (GAA→GAC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,700	0	A	C	22.3%	484.8 / 162.2	355	E181D (GAA→GAC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (155/114); new base C (60/20); total (218/137)
Fisher's exact test for biased strand distribution p-value = 5.79e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.20e-02

Only 100 of 370 total aligned reads displayed.

GCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACA  >  NZ_CP034943/607563‑607838

    cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                                                      >  1:3136927/1‑140 (MQ=255)

     gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                                                     >  1:3922394/1‑140 (MQ=255)

     gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCAGGACCACCCGGAGCGCCCGGAGCTACCGGGGAAGCCGGAc                                                                                                                                     >  2:67128/1‑140 (MQ=255)

            ccaGCGGCGACGGGGCAAGCCGGCCCACCCGGAGCGCCAGGAGCGACCGGGGAAGCCGCACCAGCCGGAGCGACCGCAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                              <  2:4103401/139‑1 (MQ=14)

              cgcggCCACCGGCGAAGCCGGACCACCCGGAGCGACAGCAGCTACCGGGGACCCCGGACCACCCGGAGCGACCGGAGCTACCGGGGAAGCCGCACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                                                                                            <  2:4426687/137‑1 (MQ=11)

                 ggCTCCCGGGGAAGCCGGACCAGCCGGCGCGTCAGGTTCTAGCGGGGTCGCCGGTCGAGCCGGTGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa                                                                                                                         <  1:7192493/140‑1 (MQ=11)

                  gctccgggggAAGCGGGCGCAGCGTGAGCGAGAGGAGCTCCAGGAGAACCCGGACCACCCGGACCAACAGGACCTACGGGAGTCCCGGGAGCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                        <  1:2623752/134‑1 (MQ=2)

                  gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                        <  1:3841069/140‑1 (MQ=40)

                  gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                        <  2:6972329/140‑1 (MQ=40)

                   cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGGAGCCGGAGCAGCCGGAGCGGCa                                                                                                                       >  2:4396241/1‑140 (MQ=14)

                   cTACTGGGGAAGCCGGACCAGCAGGAGCGACAGGAGCTACCTGGGAAGCAGGACCAGCCGGAGAGACAGGAGATAAAGGGGAAGACCGACAAACCGGAGCGCCAGGAGCTACCGGAGAATCCGGACACGCCGCAGCGAcc                                                                                                                       >  1:4061822/1‑139 (MQ=21)

                     aCTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGGGAAGCCGCACACGGCGGAGCGACAGGAGCTACCGGGGCTGCCGGGGCGGCCGCGGCGACggg                                                                                                                     >  2:1958747/1‑140 (MQ=11)

                         gggACGCCGGACCACCCGGAGCGACAGGAGCTACCGGCGCAGCCGGACCCGCCGGAGCGACAGGAGCTCACGGCGCCGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCt                                                                                                                 <  1:2196841/140‑1 (MQ=14)

                          ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                >  2:7415905/1‑140 (MQ=255)

                           gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAc                                                                                                               >  2:5260997/1‑140 (MQ=255)

                            aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                                              >  2:927265/1‑140 (MQ=255)

                            aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                                              >  2:1707905/1‑140 (MQ=255)

                            aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAACAGCCGGAGCGACAGGAGCTACCGGCGACTCCGGAACAACCGGAGCGACAGTAGCTCCCGGGGCAGCCGGAGCAGACAGAGCGACAGGaacacac                                                                                                              >  2:1118453/1‑134 (MQ=14)

                            aaGCCGGACCAACCGGAGCGACAGGAGCTACCCGGGAATCCGGACCAGCCGGAGCCACAGGAGCTACCGGGGAAGCCCGCCCAACCGGAGCGACCGGAGCAACCGGGGAAGCCGGAGCTGCCGGAGCGCCAGGAGCTccc                                                                                                              >  2:2073871/1‑140 (MQ=14)

                              gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACCGGAGCTACCgg                                                                                                            >  2:4254078/1‑140 (MQ=255)

                                   acctcccGGAGCGACCGGAGCTTCCGGGGACGCCGGAGCACCAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                       <  1:5078321/135‑1 (MQ=14)

                                   aCCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGCTCGACAGGAGCTAGCGGGGATGCCGGACGTTGCGGTGAGAGTGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                       <  1:4580674/140‑1 (MQ=14)

                                    ccACCCGGAGATACAGGAGCTCCCGGGGACCCCGGACCAGCCGGAGCCCCAGGAGCTACCGGAGAAGCCGGACCAACCGGAGCGACAGGCGCTACCGGGGAAGCGGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                      <  1:2532430/140‑1 (MQ=14)

                                       gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                              <  1:6732857/113‑1 (MQ=39)

                                            aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCAACCGGGGAAGCCGGACCAGACGGACCGACAGGAGCTACCGGGGAAACCGGACCaa                                                                                              >  1:5286077/1‑140 (MQ=255)

                                                      gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGACCAACCGGAGCGAc                                                                                    >  2:6724768/1‑140 (MQ=255)

                                                         aCCGGGGAAGCCGGACGAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGACGGACCAACCGGAGCGACggg                                                                                 <  1:7434327/140‑1 (MQ=255)

                                                         aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCAACCGGGGAAGCAGGAACAGCCGGAGCGGCCTGAGCTCCACGCGAAGCGGGTCCAACCGGAGCGACcgg                                                                                 >  2:3806238/1‑140 (MQ=14)

                                                          ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCAGCCGGAGCTCCCGGAGCGACCGGAGCCACCGGCCCAGCCGGAGCTCCGGGa                                                                                >  2:2888938/1‑140 (MQ=11)

                                                           cGGTGAAGCCGGACCAGCCGGAGCGCCAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAg                                                                               <  1:6901642/140‑1 (MQ=255)

                                                           cGGGGCAGCCGGACCACCCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCGGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAg                                                                               <  1:2008807/140‑1 (MQ=255)

                                                               gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                           <  2:3922388/140‑1 (MQ=255)

                                                               gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                           <  2:5312208/140‑1 (MQ=255)

                                                               gAACCCGGACCACCCGGAGCAACAGGACCTCCCGGGGCCGCCGGACCAACCGGAGCGACAGGAGCTCCCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                           <  1:1345719/140‑1 (MQ=14)

                                                               caacccGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                           <  2:4804837/136‑1 (MQ=255)

                                                                  gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                        >  1:5904119/1‑140 (MQ=255)

                                                                  gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGCGACGCCGGACCAGCCGGAGCGGCAGGAGCTCGCGGGGAAGCGGGACCAAACGGACCGACGGGAGCCACCgg                                                                        >  1:5800946/1‑140 (MQ=14)

                                                                       ccgaGCCGGAGCGACCGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa                                                                   <  1:1753411/137‑1 (MQ=255)

                                                                       aCCAGCGGGAGCCCCAGGAGCTACCGGCGACGCCGGAGCTCCCGGGGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGATACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa                                                                   <  2:2174182/140‑1 (MQ=14)

                                                                       aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                                                          >  2:5820955/1‑69 (MQ=35)

                                                                          aGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAACGGAGCGACGGGAGCCACAGGGGCAAcgg                                                                >  2:3703364/1‑138 (MQ=255)

                                                                            caggAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                           <  1:6127079/125‑1 (MQ=255)

                                                                                      cggAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                                    <  2:3587901/139‑1 (MQ=255)

                                                                                             cccGGGGAAGCCGGACCTACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                             <  2:1286250/139‑1 (MQ=255)

                                                                                             aCCGGGGACGCCGGCCCACCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                             <  2:6196497/140‑1 (MQ=18)

                                                                                             aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGCCCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGCCCAACCGCAGCGACGGGAGCGACAGGGGCTACAGGGGACGGCGGACCAGCCgg                                             >  2:5131697/1‑140 (MQ=17)

                                                                                             aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                  <  1:962778/87‑1 (MQ=38)

                                                                                                ggggAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGc                                          >  1:4921171/1‑140 (MQ=255)

                                                                                                  ccaccccGGACCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGa                                        <  1:1024975/135‑1 (MQ=17)

                                                                                                   gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                       <  1:3476655/140‑1 (MQ=255)

                                                                                                   gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGACCCACAGGGGCTACTGGGGACGTCTGACCAGCCGGAGCGAc                                       >  1:4307820/1‑140 (MQ=255)

                                                                                                    aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCCACAGGGGCAACCGGCGAAGGCGCACCAGCCGGAGCGACa                                      >  2:6060580/1‑140 (MQ=14)

                                                                                                    aaGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                 <  1:6625711/97‑1 (MQ=37)

                                                                                                    aaGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                 >  2:6625711/1‑97 (MQ=37)

                                                                                                     aaccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAg                                     >  2:2718191/3‑140 (MQ=14)

                                                                                                      gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                    <  2:1494821/140‑1 (MQ=255)

                                                                                                      gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                    <  2:5237475/140‑1 (MQ=255)

                                                                                                      gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGTCGGACCAGCCTGAGCGACAgg                                    >  1:7058299/1‑140 (MQ=255)

                                                                                                      accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                    >  2:701367/2‑140 (MQ=14)

                                                                                                      accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                    <  1:4423127/139‑1 (MQ=14)

                                                                                                      accGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                    >  2:4902357/2‑140 (MQ=14)

                                                                                                           agcgacgggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                               >  2:2463067/8‑140 (MQ=17)

                                                                                                           aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                               >  2:1413417/1‑140 (MQ=255)

                                                                                                           aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                               >  1:1630837/1‑140 (MQ=255)

                                                                                                              aaCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCg                            >  2:7233493/1‑140 (MQ=255)

                                                                                                                ccGGAGCGACAGGAGCTACCGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                      >  2:6803234/1‑96 (MQ=255)

                                                                                                                    aGCCACCGGAGCTACCGGTGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                      <  1:3621525/140‑1 (MQ=255)

                                                                                                                     gccaCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc                     >  1:5416058/4‑140 (MQ=18)

                                                                                                                     gccaCAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc                     >  2:535828/4‑140 (MQ=18)

                                                                                                                        aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                  <  2:3142166/140‑1 (MQ=255)

                                                                                                                        aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                  <  1:4115359/140‑1 (MQ=255)

                                                                                                                         cAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                 >  1:1467322/1‑140 (MQ=255)

                                                                                                                         cAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                 >  1:4727269/1‑140 (MQ=255)

                                                                                                                         cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                      <  1:1194869/135‑1 (MQ=255)

                                                                                                                         cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                      >  2:1194869/1‑135 (MQ=255)

                                                                                                                         cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                 >  2:7571664/1‑140 (MQ=255)

                                                                                                                              gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc            >  1:531489/1‑140 (MQ=255)

                                                                                                                              gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc            >  1:2856865/1‑140 (MQ=255)

                                                                                                                              gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc            <  2:1047687/140‑1 (MQ=255)

                                                                                                                              gCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc            <  2:3346397/140‑1 (MQ=255)

                                                                                                                              gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACAGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACACGGACCAAc            >  2:1633087/1‑140 (MQ=255)

                                                                                                                              gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAAc            >  2:4007503/1‑140 (MQ=255)

                                                                                                                                tACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCG          >  1:643551‑M1/1‑140 (MQ=255)

                                                                                                                                 actggggacgTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCCGGGAAGCCGGACCAACCGc         >  2:868399‑M1/4‑139 (MQ=255)

                                                                                                                                 actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                          <  1:523792/120‑1 (MQ=255)

                                                                                                                                  ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGt                                                         >  2:182496/1‑91 (MQ=255)

                                                                                                                                  ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGA        >  2:6275967‑M1/1‑140 (MQ=255)

                                                                                                                                  ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGA        >  2:6433519‑M1/1‑140 (MQ=255)

                                                                                                                                  ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGA        >  2:54518‑M1/1‑140 (MQ=255)

                                                                                                                                  ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGA        <  1:927265‑M1/140‑1 (MQ=255)

                                                                                                                                  ccGCGGAAGCCGGCCCAGCCGGACCGACAGGCGCTCCCGGGGATTCCGGCCAAACCGGAGCGTCGGGAGCCACAGGTGCTACTGGGGACGGCGGAACAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGA        <  1:2073871‑M1/140‑1 (MQ=255)

                                                                                                                                      ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGa    >  1:3264410/1‑140 (MQ=255)

                                                                                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                          <  2:4702809/69‑1 (MQ=255)

                                                                                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                 <  2:5568691/126‑1 (MQ=255)

                                                                                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                 >  1:5568691/1‑126 (MQ=255)

                                                                                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc   >  1:3346397/1‑140 (MQ=255)

                                                                                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc   >  1:2724281/1‑140 (MQ=255)

                                                                                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc   >  1:844064/1‑140 (MQ=255)

                                                                                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc   <  2:3264410/140‑1 (MQ=255)

                                                                                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACT‑GGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa  >  2:5023963/1‑140 (MQ=255)

GCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACA  >  NZ_CP034943/607563‑607838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`