Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 634,052 T→G 15.5% E573D (GAA→GAC EO946_RS03330 ← ABC‑F family ATP‑binding cassette domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943634,0520TG15.5% 627.7 / 44.5 324E573D (GAA→GACEO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (91/172);  new base G (59/2);  total (150/174)
Fisher's exact test for biased strand distribution p-value = 7.13e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 339 total aligned reads displayed.

GTCTGCATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAA  >  NZ_CP034943/633914‑634187
                                                                                                                                          |                                                                                                                                       
gTCTTCATGGATGGTTTGTACATTTTCATTTTCTTGATAGATTTCCTGATAACATAGCAATTCTTATTTATTACTAATGTTTTCTTCAATGGTTTTTACATTTTATTCTTTTTATTAAATAATACGGAgtcgttgtcgtt                                                                                                                                        <  2:4076493/140‑1 (MQ=255)
gTCTGCATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGtt                                                                                                                                        <  1:5319074/140‑1 (MQ=255)
 tCTGCATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGttc                                                                                                                                       >  1:1531620/1‑140 (MQ=255)
     cATGGATGGCTTGTACCTTTTCATGGTCATGATAGATTTCCGGATCACATAGCAATACATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATACGACGGAGTCGTTGGCGttctttc                                                                                                                                   >  2:4147172/1‑140 (MQ=255)
           tggCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTGttttta                                                                                                                             >  1:5597559/1‑139 (MQ=255)
            ggCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTcc                                                                                                                            <  2:1385767/140‑1 (MQ=255)
             gCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGCGGTGTTTGTTTTTGCa                                                                                                                           >  1:3277983/1‑140 (MQ=255)
              cTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCAt                                                                                                                          <  1:3981814/140‑1 (MQ=255)
               ttGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGGTTCTTTTTACAtt                                                                                                                         >  2:1177976/1‑140 (MQ=255)
               ttGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTGTTTTTCCAtt                                                                                                                         >  1:4467607/1‑140 (MQ=255)
                 gTACCTTTTCATGGTCTTGATAGATTTCCGGATCACCTAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCt                                                                                                                       <  2:3599159/140‑1 (MQ=255)
                 gTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCt                                                                                                                       <  2:5351152/140‑1 (MQ=255)
                   aCCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCttt                                                                                                                     <  2:912823/140‑1 (MQ=255)
                    ccTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTCTATCTTTTTCCATGGtttt                                                                                                                    >  2:6153398/1‑140 (MQ=255)
                    ccTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCtttt                                                                                                                    >  2:833272/1‑140 (MQ=255)
                      ttttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTttc                                                                                                                  <  1:4858438/140‑1 (MQ=255)
                       tttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTTATTTTTGGATTAttttttt                                                                                                                 >  1:2005641/1‑138 (MQ=255)
                       tttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGTTGTTTGTTTTTGGAGTCtttttgt                                                                                                                 >  1:5888716/1‑138 (MQ=255)
                       tttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTATTCAATCAGACGGAGTCGTTTGCGTTCTTTATTTTTACATTATTTttct                                                                                                                 >  2:1946093/1‑140 (MQ=255)
                       tttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAAAAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGCGTTGTTTCTTTTTCCAATAtttttgt                                                                                                                 >  2:7099507/1‑138 (MQ=255)
                        ttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGCGTTGTTTGTTTTTACATTGTTTttctt                                                                                                                >  2:2612948/1‑140 (MQ=255)
                        ttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTGTTTTTCCAATATTTttatt                                                                                                                >  1:1668017/1‑140 (MQ=255)
                        ttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTttctt                                                                                                                <  2:7303628/140‑1 (MQ=255)
                               tCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGGTGTTTGTTTTTGCATTGTTTTTATTATTAata                                                                                                         >  1:4320254/1‑140 (MQ=255)
                               tCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGCGGTGTTTGTTTTTGCATTCTTTTTGTTGTTAata                                                                                                         >  1:1812351/1‑140 (MQ=255)
                                 ttGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATg                                                                                                       <  1:3423450/140‑1 (MQ=255)
                                 ttGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATg                                                                                                       <  1:7571363/140‑1 (MQ=255)
                                 ttGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATg                                                                                                       <  1:6238887/140‑1 (MQ=255)
                                 ttGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTCTTTTTCCATTATTTTTATTCTTCATATg                                                                                                       >  2:4389242/1‑140 (MQ=255)
                                 ttGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCAGACGGAGTCGTTGGCGTTGTTTGTTTTTTCAATATTTTTATTATTCATATg                                                                                                       >  2:4570/1‑140 (MQ=255)
                                   gATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGaa                                                                                                     <  2:7643653/140‑1 (MQ=255)
                                       taTTTGCTGATCAGATATCAATTCATCGTTATGAATAATGTTTTTTTCAATGGTTTGATGAGTGGCTTCAATTTATTGAATCCTATGGAGTCGTTTGAGTTCTTTCTTTTTCCATTCTTTTTTTTTTTCTTTTGATCGtt                                                                                                 <  2:4507426/139‑1 (MQ=255)
                                       gATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACCGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGtt                                                                                                 <  2:3762330/140‑1 (MQ=255)
                                       gATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGtt                                                                                                 <  1:1875003/140‑1 (MQ=255)
                                       gATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGtt                                                                                                 <  1:848199/140‑1 (MQ=255)
                                       gATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCAGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGtt                                                                                                 <  1:7678667/140‑1 (MQ=255)
                                        aTTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGttt                                                                                                <  2:6343872/140‑1 (MQ=255)
                                        aTTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGttt                                                                                                <  2:7228831/140‑1 (MQ=255)
                                         tttCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGCGTTGTTTGTTTTTTCAATCTTTTTGTTGTTCATAATAAAGtttt                                                                                               >  1:1188945/1‑140 (MQ=255)
                                         tttCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGTTTGTTTTTACATTCTTTTTATTGTTCAAATTAAACtttt                                                                                               >  1:3646447/1‑140 (MQ=255)
                                          ttCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTCGTTTTCCATAATTTTTATTCTTGATAGTGACGttttt                                                                                              >  2:4000634/1‑139 (MQ=255)
                                           tCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTTCTTTTTCCATTATTTTTATTCTTAATATTAAAGTTTTGa                                                                                             >  2:7113506/1‑140 (MQ=255)
                                           tCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTTCTTTTTACATTGTTTTTCTTATTCATATGTAAGttttta                                                                                             >  2:6615350/1‑138 (MQ=255)
                                           tCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTGCTTTGTTTTTGCATTCTTTTTCTTATTAATATGAACGTTTTGa                                                                                             >  2:2394522/1‑140 (MQ=255)
                                           tCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGGTTCTTTTTACAATATTTTTATTATTAATATGAAATttttta                                                                                             >  2:6551313/1‑138 (MQ=255)
                                           tCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTATTCTTGATATTAACGTTTTGa                                                                                             >  2:3872042/1‑140 (MQ=255)
                                             cGGATCACATAGCAATTCATCGTTATGACTAATTTTTTCTTCCATTGTTTGAACAGTGGGTTCAATTTCTTCAATCCGACGGAGTCGTTTGCGTTCTTTCTTTTTCCATTCTTTTTCGTCTTCATATGAACGTTTTGAGt                                                                                           <  2:2920643/140‑1 (MQ=255)
                                             cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCACTGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                           <  1:4639966/140‑1 (MQ=255)
                                             cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                           <  1:2791050/140‑1 (MQ=255)
                                             cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                           <  2:5269327/140‑1 (MQ=255)
                                             cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                           <  2:5608450/140‑1 (MQ=255)
                                             cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTACATCCGACGGAGTCGTTTGCGTTCTTTCTTTTTCCATTCTTGTTCTTCTTCATATGAACGTTTTGAGt                                                                                           <  1:710479/140‑1 (MQ=255)
                                             cGGACCACATACCACTTCATCGTTCTGACTAATGTTGTCTTCAATTGTTTGAACAGAGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCGTCATATGAACGTTTTGAGt                                                                                           <  1:6447534/140‑1 (MQ=255)
                                             cGGAACACATAGCACTTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACACTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                           <  1:3824466/140‑1 (MQ=255)
                                                aTCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGCGTTGTTTGTTTTTGCATTGTTTTTGTTGTTCATATGAACGTTTTGTGTAAg                                                                                        >  2:2964689/1‑140 (MQ=255)
                                                            ttCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTCTTTCTTTTTACATTCTTTTTGTTCTTCATATGAACGTTTTTAGTAAGGCGTAACCGAAg                                                                            >  2:3097879/1‑140 (MQ=255)
                                                            ttCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTGTTTTTACATTGTTTTTGTTGTTCATATGAAAGTTTTGAGTCAGGCTTAAACGAAg                                                                            >  2:5129776/1‑140 (MQ=255)
                                                               aTCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTATTTTTCCATTGTTTTTCTTCTTCATATGAACGTGTTTAGTCAGGCGTAAACCAAGCAg                                                                         >  2:7052960/1‑140 (MQ=255)
                                                                 cGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGTTTGTTTTTGCAATGTTTTTGTTGTTGATATTAACCTTTTTATTGAGGCTTAAACCAAGCAggg                                                                       >  1:6024957/1‑139 (MQ=255)
                                                                  gTTATGACTAATGTTTGCGTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCGTTCATTTTCCATTCTTTTTCTTCTTCATATGCACGTTTTGACTCAGGCTTAACCGAAGCAGGCg                                                                      <  1:1090621/140‑1 (MQ=255)
                                                                   ttATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTCTTTTTGCATTGTTTTTCTTCTTCATATGAACGTTTTGTGTCTGGCTTAACCGTAGCAGGCGt                                                                     >  2:7394167/1‑140 (MQ=255)
                                                                        aCTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGCGTTGGTTTTTTTTGGATTGTTTTTGTTTTTGATATTTAAGTTTTTTTTGAGGCTTAAACCAAAGACGCGgttttt                                                                >  1:4703443/1‑140 (MQ=255)
                                                                           aaTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCgg                                                             <  2:3744438/140‑1 (MQ=255)
                                                                           aaTGTTTTCTTCAATGGTTTGAACAGTGGCGTCAATTTGTTCAATCCGACGGAGTCGTTGGGGGTGTTTTTTTTTTCAATATTTTTTTTGTTGATTATTACGTTTTTTTTAAGGCGTAAACCAAACAGGCGgtttttgag                                                             >  1:7316204/1‑137 (MQ=255)
                                                                            aTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGa                                                            <  2:1645214/140‑1 (MQ=255)
                                                                             tGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGCGTTCTTTCTTTTTGCATTGTTTTTGTTCTTGATATTAAAGTTTTGAGTCAGGCTTAAACGAAGCAGGCGTTTTTTAGGAt                                                           >  2:6121532/1‑140 (MQ=255)
                                                                             tGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTCTTTTTGCATTCTTTTTCTTCTTCAAATGAACGTTTTTAGTGAGGCTTAACCCAAGCAGGCGTTTTTTAGGAt                                                           >  2:1877947/1‑140 (MQ=255)
                                                                             tGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTATTTTTGCAATCTTTTTCTTCTTCATATGAACGGTTTGGGTCAGGCGTAACCGAATCAGGCGTTTTTTgggtt                                                           >  2:6854254/1‑138 (MQ=255)
                                                                             tGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATACGACGGAGTCGTTGGGGTTATTTATTTTTACATTCTTTTTGTTCTTCATATGAACGTTTTTAGTCAGGCTTAAACGAAGCAGGCGTTTTTTGGGAt                                                           >  2:3649807/1‑140 (MQ=255)
                                                                                 ttcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCct                                                       <  2:6976183/140‑1 (MQ=255)
                                                                                 ttcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTATTTATTTTTGCCTTCTTTTTCTTCTTGATATTAAAGTTTTGAGTAAGGCTTAAACCAAGCACGCGTGTTTTCGGATTCct                                                       >  2:4719045/1‑140 (MQ=255)
                                                                                              tGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTGTTTTTGCATTGTTTTTGTTGTTCATATTAACGTTTTGAGTGAGGGTTTACCGAATCACGCGTTTTTTTGTATTCCTCTTTGTGTTTCAt                                          >  2:6423837/1‑140 (MQ=255)
                                                                                              tGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTGCCTTCTTTTTCTTCTTCATATGAACGTTTTGAGTGAGGCTTAAACGAACCAGGCGTTTTTTGGGATTGCTCTTTGTGATTAAt                                          >  2:508264/1‑140 (MQ=255)
                                                                                              tGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTATTCTTCATATTAACGTTTTGAGTCAGGCTTAAACGAAGAAGGCGTTTTTTAGGAATACTCGTTCTGTTTGAt                                          >  2:2501378/1‑140 (MQ=255)
                                                                                                aaCAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGCGGTGGTTATTTTTGGATTATTTTTTTTGTTAAAATTTACGTTTTTATTTAGGCTTAAAAGAACCAGGCGTTTTTTGGGATTGCTGTTTGTGAtttcttt                                        >  1:6731458/1‑135 (MQ=255)
                                                                                                   aGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTtct                                     <  2:3295557/140‑1 (MQ=255)
                                                                                                    gTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTtctc                                    <  1:2600901/140‑1 (MQ=255)
                                                                                                    gTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTtctc                                    <  1:3507264/140‑1 (MQ=255)
                                                                                                     tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTCTTTGTTTTTCCATTATTTTTCTTGTTAATATGAAAGTTTTTAGTAAGGCGTAAACGAAGCAGGCGTTTTTTAGGATTGCTCTTGCGTATTAAttttatac                                   >  2:3507264/1‑136 (MQ=255)
                                                                                                     tGGCTTCAATTTCTTCAATCAGACGTAGTCGTATGCGTTGTTTAATTTTCCAATGTTTTTCTTCTTAAAAAGAACGTTTTGACTCAGGCTTAAACGCAGACTGCGGTTTTTAGGATTGCTCTTGCTGATTCAttttgtga                                   >  2:2263162/1‑136 (MQ=255)
                                                                                                        cTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAAt                                <  2:2299007/140‑1 (MQ=255)
                                                                                                           cAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCa                             <  2:25993/140‑1 (MQ=255)
                                                                                                                  ttcAATCCGACGGAGTCGTTGGCGTTGTTTCTTTTTGCCATCTTTTTATTCTTGATATTAACCGTTTGTGTCAGGCTTAAACCAATCAGGCGTTTTTTGGGATTACTCTTGGTGATTGATTTTGGACAAATCAAgctggg                      >  2:3045453/1‑138 (MQ=255)
                                                                                                                  ttcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                      <  2:7136507/140‑1 (MQ=255)
                                                                                                                  ttcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                      <  1:1329192/140‑1 (MQ=255)
                                                                                                                  ttcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTGTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                      <  2:2090238/140‑1 (MQ=255)
                                                                                                                  ttcAATCCGACGGAGTCGTTGGCGGTGTTTGTTTTTCCATTGTTTTTCTTCTTCATAGGAAAGGTTTTAGTCACGCTTAACCCAAGCAGGCGGTTTTTAGGATTCCGCTTGGTGGTTGATTTTGTACAAATAAAactggg                      >  2:77104/1‑138 (MQ=255)
                                                                                                                    cAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGctct                    <  2:2428444/140‑1 (MQ=255)
                                                                                                                    cAATCCGACGGAGTCGTTGGCGTTATTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGctct                    <  2:1331438/140‑1 (MQ=255)
                                                                                                                         cGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGtttt               <  2:4636247/140‑1 (MQ=255)
                                                                                                                         cGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGtttt               <  2:1284372/140‑1 (MQ=255)
                                                                                                                            cGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCGTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt            <  2:4300202/140‑1 (MQ=255)
                                                                                                                              gAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtct          <  2:595827/140‑1 (MQ=255)
                                                                                                                              gAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtct          <  2:1761212/140‑1 (MQ=255)
                                                                                                                              gAGTCGTGGGCGTTCTTTCTTTGTCCCTTCTTTTTCGTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtct          <  1:4114131/140‑1 (MQ=255)
                                                                                                                                 tCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCg       <  1:2417280/140‑1 (MQ=255)
                                                                                                                                 tCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCg       <  1:972425/140‑1 (MQ=255)
                                                                                                                                 tCGTTGGCGTTCTGTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCg       <  1:2302183/140‑1 (MQ=255)
                                                                                                                                   gTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGta     <  2:2810094/140‑1 (MQ=255)
                                                                                                                                     tGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGtata   <  1:7166542/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                       
GTCTGCATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAA  >  NZ_CP034943/633914‑634187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: