Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 112,660 C→A 5.2% T12K (ACA→AAA)  cysE → serine O‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943112,6600CA 5.2% 1928.7 / 24.2 673T12K (ACA→AAA) cysEserine O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (174/433);  new base A (60/2);  total (236/437)
Fisher's exact test for biased strand distribution p-value = 1.57e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 675 total aligned reads displayed.

ACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAAACGAAAATTTTATTTCCTTGCACGCCTTATATCT  >  NZ_CP034943/112521‑112799
                                                                                                                                            |                                                                                                                                           
        gAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGTATAGGAAATCAAAGAGAGAAGGGGGGGGAAGCAGGTGTTGTTTATACTTCTCAAAGACGACAATGATAAAGTGTTCg                                                                                                                                     >  1:6621358/1‑140 (MQ=255)
            cgcgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGCAGACATTGATACAGTGTTCGATCa                                                                                                                                 <  2:3748390/140‑1 (MQ=255)
              cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGCGGCGCTTTTTTTATTTGGGGTAGACGAAATGAAATATTGAATGGGGGGGACGCCTGTGTTTTTTTGAATTCTGTAAGAAACAATTGATACCGTGTTAGGTCAAg                                                                                                                               >  1:100097/1‑140 (MQ=255)
              cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGATGCGCGGTTTTTATTTGGTGAATAGGAAATAAAATATATAAGTGGGGGGCAGCATTTGGTTTTTTTAATGATAAAAAAACACAATGATAAATTGTTCAATaaaa                                                                                                                               >  1:7107749/1‑139 (MQ=255)
              cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGAAGAGGAAATCAAATATAGAACTGGGGGGAAGCCTGTGTTTTTTAGAATGCTAAAAGAAGACATTGATACACTGTTCGaaaaaa                                                                                                                               >  1:5953952/1‑135 (MQ=255)
              cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGAAGAGGAAAACAAAAAGAGAAGTGGGGGGAACCCAGTGTTTTTTATTATTCTCAAATAAAAAATTGATAAAGTGTTCGATAAAg                                                                                                                               >  1:7504877/1‑140 (MQ=255)
              cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGTGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATTCTCAAAGAAGACATTGATACAGTGTTAGATCAAg                                                                                                                               >  2:5136207/1‑140 (MQ=255)
              cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATTCTCAAAGAAGACAATTATAAATTGTTCGATaaaa                                                                                                                               >  2:5827986/1‑139 (MQ=255)
              cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTGTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAATCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTAGATAAAg                                                                                                                               >  2:2620410/1‑140 (MQ=255)
              cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTTTTTTAGTGGGTGACGAGGAAATCCAAAAGCTTAGTGGGGGGGAGCATGTGTGGTTTATAATGCTCAAAAAAGACATTGATAAAGGTTTGGATAAAg                                                                                                                               >  1:958729/1‑140 (MQ=255)
              cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGGTTTTTATTGGGGGTAGATGTCATCCAAAAGTGAAGTGGGGGGAACCCATTGTTTTTTTGGAAGGTCAAATGATACATATATAAATTGTTCGATaaat                                                                                                                               >  1:4011992/1‑139 (MQ=255)
                   tttAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCca                                                                                                                          <  2:3080304/140‑1 (MQ=255)
                             tctGTCATGTTTTCATGCCGAGGCGCTTTTTTTATTTGGTGTAGATGGAATCAAATAGAGTAGTGGGGGGAAGGATGTGTTTTTTTGTATGCTCAAAGAAGACCTTGTTAAATTGTTCGATCAAGATCCCGCAGCaagaa                                                                                                                >  1:1909300/1‑140 (MQ=255)
                             tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTTGGTGAAGAGGGAATCAAATAGAGAAGTGGGGGGAAGCCAGTGTTTTTTAGAATTCTCAAAGAAGACATTGTTAAAGTTTTGGATGAAGATCCAGCAGCaagaa                                                                                                                >  1:5506279/1‑140 (MQ=255)
                             tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTTGGGGTATACGCAAAACAAAAGAGCAGGGGGGGGGAGCATGTGTTTTTTTTGATGCGGAAAAAAGACAATGAGAAACTGTTAGGTAAAAATCCACCAGCaacca                                                                                                                >  1:2997412/1‑137 (MQ=255)
                             tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTTGGGGAAGAGGAAATAAAAAACAGAAGTGGGGGGAAGCATGTGTTTTTTATAATTCTCAAAGAACACAATTATAAAGTGTTAGATCAAGATACATCACCaagaa                                                                                                                >  1:3568391/1‑140 (MQ=255)
                             tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGTAAAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAATAAGACATTGATACAGTTTTCGATCAAAATACAGCAGCaagaa                                                                                                                >  1:5642382/1‑140 (MQ=255)
                             tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAATCATGTGTTTTTTAGAATGCTGAAAGAAGACATTGATACAGTGTTAGATCAAGATACAGCAGCaagaa                                                                                                                >  2:7580319/1‑140 (MQ=255)
                             tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTATAATTCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaa                                                                                                                >  2:7167810/1‑140 (MQ=255)
                             tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTATAATGCTCAAAGAATACATTGATACAGTGTTAGATCAATATCCAGCAGCaagaa                                                                                                                >  2:3643970/1‑140 (MQ=255)
                             tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAAGAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCaagaa                                                                                                                >  2:4771010/1‑140 (MQ=255)
                             tctGTCATGTTTTCATGCAGAGGCGCTGTTTTTATTGGGTGAAGAGGGAATCAAAAAGAGACGTGGGGGGGAGCATGTGTTTTTTAGAATGCTCCAAGTATACATTGTTAACGTGTTGGATCGAGATCCAGCCGCaagaa                                                                                                                >  2:5565106/1‑140 (MQ=255)
                             tctGTCATGTTTTCATGCAGAGGCGCGTTTTTTATTTGGTGTAGAGGCAAAAAAAAATATAAGTTGGGGGACGCATGTGTTTTTTTTAATTCTCAAAAAAGAAAATGAAAAAGTGTTCGATAAAAATACAGCAGCaaaaa                                                                                                                >  1:1280956/1‑140 (MQ=255)
                             tctGTCATGTTTTCATGCAGAGGCGCGTTTTTTATTTGGGGTATAGGTAATAAAATATAGTAGTGGGGGGAATCATGTTTTTTTTTTAAATGTCAAAGTATACAATTATAAATGGTTCGATAAATAACCAGCAGcaacaa                                                                                                                >  1:7418886/1‑140 (MQ=255)
                                 tCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACAATGATACAGTGTTCGATAAAGATACAGCAGCAAGAAGTTa                                                                                                            >  2:618411/1‑140 (MQ=255)
                                        ttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGaa                                                                                                     <  1:4714143/140‑1 (MQ=255)
                                        gtCATGCAGAGGCGCTTTTTATATTGGCTGAAGAGCAAACCAAATAGAGAAGGGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATtgtgca                                                                                                     <  1:4322063/139‑3 (MQ=255)
                                         tCATGCAGAGGCGCTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGt                                                                                                   <  1:827684/140‑1 (MQ=255)
                                                      cTTTTTTTTTTGGGTGTAAAAGAAATGAAATACAGAAGTGGGGGGAATCAAGTGTTTTTTAGAAAGATCAAAAAAAACATAGTTACAGTGTTCGATCAATAAACAGCAGCAAGAAGTTATTTTGGAGTGTATTTAAATTa                                                                                       >  2:2792227/1‑140 (MQ=255)
                                                      cTTTTTTTATTTGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTAAAAGAACAAAATTATACACTTTTCGAACAAAATACAGCATCAAAAAGTTATTTTTAATTGTTTTTAAATTa                                                                                       >  2:6914540/1‑140 (MQ=255)
                                                        ttttttCTTGGGTGCAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTAtt                                                                                     <  1:5914017/140‑1 (MQ=255)
                                                         tttttATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTc                                                                                    <  1:3672659/140‑1 (MQ=255)
                                                         tttttATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTc                                                                                    <  1:2723308/140‑1 (MQ=255)
                                                         tttttATTGGGTGAAGAGGAAATCAAAGAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTc                                                                                    <  2:2294317/140‑1 (MQ=255)
                                                         ttgttATGGGGTGAAGAGGAAATCAACTCGCGAAGTGGGGGCAAGCATGTGTTTTTTAGAATGCTCAACGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGCAGGGATTTTAACTTATTc                                                                                    <  1:3274818/137‑1 (MQ=255)
                                                         tatttATTGGGTGAAGAGGACATCACATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACAGTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTc                                                                                    <  2:3550870/138‑1 (MQ=255)
                                                          ttttCTGGGGAGCAGCGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACAGTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTcc                                                                                   <  1:5109060/140‑1 (MQ=255)
                                                           tttATTGGGTGAAGAGGAAATCAAATAGAGAACTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCg                                                                                  <  2:3250646/140‑1 (MQ=255)
                                                               ttGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGttt                                                                              <  2:1346828/140‑1 (MQ=255)
                                                                tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa                                                                             <  2:7503797/140‑1 (MQ=255)
                                                                tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa                                                                             <  2:2114352/140‑1 (MQ=255)
                                                                tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa                                                                             <  2:6348808/140‑1 (MQ=255)
                                                                 gggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTAc                                                                            >  1:2532487/1‑140 (MQ=255)
                                                                  ggTGAAGAGGAACTCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                                           <  2:4344464/140‑1 (MQ=255)
                                                                  ggTGAAGAGGAAATCCAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                                           <  1:2279706/140‑1 (MQ=255)
                                                                  ggTGAAGAGGAAATCACATAGAGAAGTGGGGGGAAGCATGTGTGTTTTAGAATGCTCAAAGAAGACATTGATACAGTGGTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                                           <  1:2084139/140‑1 (MQ=255)
                                                                  ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                                           <  2:2953168/140‑1 (MQ=255)
                                                                  ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                                           <  1:7216172/140‑1 (MQ=255)
                                                                  ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                                           <  1:4609061/140‑1 (MQ=255)
                                                                  ggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                                           <  1:6083766/140‑1 (MQ=255)
                                                                  gctGAAGCGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACa                                                                           <  1:5794909/138‑1 (MQ=255)
                                                                    tGAAGAGGAAATCAAATAGAGAAGTGTGCGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTGTGAAGTGATTTTAACTTATTCCGGTTTACATg                                                                         <  2:2497325/140‑1 (MQ=255)
                                                                    tGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATg                                                                         >  2:4475933/1‑140 (MQ=255)
                                                                    tGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATg                                                                         <  2:95929/140‑1 (MQ=255)
                                                                    tGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATg                                                                         <  1:6992939/140‑1 (MQ=255)
                                                                    agAAGACGAAATCAAAGAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCACAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATg                                                                         <  2:5400356/139‑1 (MQ=255)
                                                                         agGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCtata                                                                    <  1:3776408/140‑1 (MQ=255)
                                                                             aaaTCAAATAGAGAAGTGGGGGGACGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGAAACAGTGTTCGATCAATATCCAGCAGCAAGAAGTTATTTTTAAGTGATTTTAACTTATTCCGGTTTACATGCTATATgg                                                                 >  2:245188/1‑140 (MQ=255)
                                                                               aTCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCgg                                                                                 >  1:1723526/1‑122 (MQ=255)
                                                                               aTCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGgc                                                               >  2:4650744/1‑140 (MQ=255)
                                                                                tCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGgcg                                                              <  2:4268940/140‑1 (MQ=255)
                                                                                  aaaTAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCa                                                            <  1:3436589/140‑1 (MQ=255)
                                                                                  aaaTAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGGGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCa                                                            <  1:114874/140‑1 (MQ=255)
                                                                                    aTAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATc                                                          >  1:1623343/1‑140 (MQ=255)
                                                                                       gagaCGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGCAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGa                                                       <  1:4193666/140‑1 (MQ=255)
                                                                                       gagaAGTGGGGGTAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGa                                                       <  1:6840138/140‑1 (MQ=255)
                                                                                       gagaAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGa                                                       <  2:1637263/140‑1 (MQ=255)
                                                                                       gagaAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGa                                                       <  2:707755/140‑1 (MQ=255)
                                                                                       gagaAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGa                                                       <  2:6264141/140‑1 (MQ=255)
                                                                                       gagaAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGa                                                       <  2:1736151/140‑1 (MQ=255)
                                                                                       gagaAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGa                                                       <  2:4011992/140‑1 (MQ=255)
                                                                                       gagaAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGa                                                       <  2:1266656/140‑1 (MQ=255)
                                                                                        agaAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGAt                                                      >  1:2048461/1‑140 (MQ=255)
                                                                                          aaGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTg                                                    >  2:5655262/1‑140 (MQ=255)
                                                                                           aGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGc                                                   >  1:4826921/1‑140 (MQ=255)
                                                                                            ggggggggAAGCATGTGTTTTTGAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGca                                                  <  2:2064305/138‑1 (MQ=255)
                                                                                             tGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGcac                                                 <  2:7367915/140‑1 (MQ=255)
                                                                                             tGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGcac                                                 <  2:3368087/140‑1 (MQ=255)
                                                                                             tGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGcac                                                 <  1:3159860/140‑1 (MQ=255)
                                                                                             tGGGCGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGcac                                                 <  1:2837960/140‑1 (MQ=255)
                                                                                             gggggggAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGcac                                                 <  1:6389353/139‑1 (MQ=255)
                                                                                                  ggcaGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCt                                            <  1:6188686/137‑1 (MQ=255)
                                                                                                       cATGTGTTTTTTAGAATGCTCAAATAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTACGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTtata                                       >  1:3601732/1‑140 (MQ=255)
                                                                                                       cATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTtata                                       >  2:6742509/1‑140 (MQ=255)
                                                                                                           tgtTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGAtt                                                                                               <  1:7616201/80‑1 (MQ=255)
                                                                                                           tgtTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTACGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATaaa                                   >  2:4971979/1‑140 (MQ=255)
                                                                                                                       tGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAAACGAAAATTTTAt                       >  2:1199352/1‑140 (MQ=255)
                                                                                                                                aagaCATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAAACGAAAATTTTATTTCCTTGCa              <  2:4132532/140‑1 (MQ=255)
                                                                                                                                aagaCATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAAACGAAAATTTTATTTCCTTGCa              <  2:2898124/140‑1 (MQ=255)
                                                                                                                                aagaCATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAAACGAAAATTTTATTTCCTTGCa              <  1:1397348/140‑1 (MQ=255)
                                                                                                                                aagaCATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAAACGAAAATTTTATTTCCTTGCa              <  2:6026895/140‑1 (MQ=255)
                                                                                                                                aagaCATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAAACGAAAATTTTATTTCCTTGCa              <  1:5409374/140‑1 (MQ=255)
                                                                                                                                aagaCATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAAACGAAAATTTTATTTCCTTGCa              <  2:7762050/140‑1 (MQ=255)
                                                                                                                                aagaCATTGATACAGTGGTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAACCGAAAATTTTATTTCCTTGCa              <  2:1883004/140‑1 (MQ=255)
                                                                                                                                aagaCATTGATACAGTGGTCGATCAAGATCCAGCAGCAAGAAGTGATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAACCGAAAATTTTATTTCCTTGCa              <  1:2397650/140‑1 (MQ=255)
                                                                                                                                 agaCATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAAACGAAAATTTTATTTCCTTGCAc             <  1:4929269/140‑1 (MQ=255)
                                                                                                                                  gaCATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAAACGAAAATTTTATTTCCTTGCACg            <  1:4439381/140‑1 (MQ=255)
                                                                                                                                   aCATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAAACGAAAATTTTATTTCCTTGCACGc           >  2:1414739/1‑140 (MQ=255)
                                                                                                                                      ttGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAAACGAAAATTTTATTTCCTTGCACGCCtt        <  2:1042281/140‑1 (MQ=255)
                                                                                                                                       tGATACTGTGTTCTATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAAACGAAAATTTTATTTCCTTGCACGCCTta       <  1:6274150/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
ACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACATGCTATATGGGCGCATCGGATTGCACATGCTTTATATAAACGAAAATTTTATTTCCTTGCACGCCTTATATCT  >  NZ_CP034943/112521‑112799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: