BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	886,736	G→C	6.8%	S51* (TCA→TGA)	EO946_RS04490 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	886,736	0	G	C	6.8%	795.2 / 6.0	303	S51* (TCA→TGA)	EO946_RS04490	MFS transporter
Reads supporting (aligned to +/- strand): ref base G (172/100); new base C (2/25); total (174/129)
Fisher's exact test for biased strand distribution p-value = 1.07e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 316 total aligned reads displayed.

CAAAGCAACAAGCAAAGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAA  >  NZ_CP034943/886597‑886875

cAAAGCAACAAGCAAAGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATg                                                                                                                                             <  2:2414299/140‑1 (MQ=255)

cAAAGCAACAAGCAAAGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATg                                                                                                                                             <  1:7477448/140‑1 (MQ=255)

  aaGCAACAAGCAAAGAACCAAGTCCGAACAGGCAAACCTCAATGATAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGGTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGAc                                                                                                                                           <  1:3820078/140‑1 (MQ=255)

  aaGCAACAAGCAAAGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGATGGTAATGCCCCATGAc                                                                                                                                           >  1:534672/1‑140 (MQ=255)

  aaGCAACAAGCAAAGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATg                                                                                                                                             >  2:2508029/1‑138 (MQ=255)

  aaGCAACAAGCAAAGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATg                                                                                                                                             <  1:2508029/138‑1 (MQ=255)

  aaGCAACAAGCAAAGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGAc                                                                                                                                           >  2:5378002/1‑140 (MQ=255)

  aaGCAACAAGCAAAGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGAc                                                                                                                                           >  1:2187232/1‑140 (MQ=255)

  aaGCAACAAGCAAAGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGAc                                                                                                                                           >  2:683721/1‑140 (MQ=255)

  aaGCAACAAGCAAAGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGAc                                                                                                                                           <  1:27430/140‑1 (MQ=255)

   aGCAACAAGCAAAGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTCAGGCCGAGCGTATAGAGGGTAATGACCCAGGAcc                                                                                                                                          >  1:6475876/1‑140 (MQ=255)

   aGCAACAAGCAAAGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAACGCCGAGCGTATAGAGGGTAATTCCCCATGAcc                                                                                                                                          >  1:2565293/1‑140 (MQ=255)

     ccacaaGCAAAGACCCAACCCCGACCAGGCAAACCTCAATCCGCAAAACGTACTGTCGGCCGTCGCGGTCGGAACGCTTGCGCCCGATCCGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCAGCAcccc                                                                                                                                        <  2:5995720/138‑1 (MQ=255)

        caaGCAAAGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCag                                                                                                                                     >  2:4930259/1‑140 (MQ=255)

        caaGCAAAGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCag                                                                                                                                     >  2:1784256/1‑140 (MQ=255)

               aGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACGGACCCGAAGGCCGAGCGGATAGAGGGTCATGCCCCAAGACCCCCACGAAGgag                                                                                                                              >  1:2085921/1‑140 (MQ=255)

                  aCCAAGTCCGAACAGGCAAACCTCAATCAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGaaa                                                                                                                           <  2:343476/140‑1 (MQ=255)

                      aGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGGATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACCGc                                                                                                                       >  1:3195060/1‑140 (MQ=255)

                           gAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTCTAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACg                                                                                                                  >  2:3577380/1‑140 (MQ=255)

                           gAACAGGCAAACCTCAATGAGAAAAAGTTTATTTCTGACGTAGCGGTCGGAAAGCTTTCCGACGATAGGCACACTGACGGTAAGGCCGAGCGTAGAGAGGGTAATGCCCCATGACACCCAGGAAGGAGAAACGGCAAACg                                                                                                                  >  1:58951/1‑140 (MQ=255)

                                      ccTAAATCACAAAAATTTTCTTTCTGCCGTAGCGGTGGGAAAGCTTTCCGACGATCGGCACACGGACGCTAAGGCCGAGCTTATAGCGGGTAATCCCCCAGGACCCCCAGGAAGCACAACCGGAAAACGACTCGTTTATc                                                                                                       <  2:2197081/140‑1 (MQ=255)

                                           aTGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGGATAGAGGGTAATGCCCCATGACCCCCAGGCAGGAGAAACGGCAAACGACTCGTTTATCgttgt                                                                                                  >  1:469179/1‑140 (MQ=255)

                                                 aactgtttgtttCTGGGGTAGGGGTCGTAAAGGTTTCCGACGCTCGGAACCGGGCCGGTACGGCCGCGTTTATTGTGTGTACGGCCCCATGACCCCCAGCACGGACAACCGGAACACGACTCGTTTATCGTTGTTAAagc                                                                                            <  2:2085921/136‑1 (MQ=255)

                                                 aaaaGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCACACGACTCGTTTATCGTTGTTAAagc                                                                                            >  2:649155/1‑140 (MQ=255)

                                                 aaaaGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAagc                                                                                            >  2:4093946/1‑140 (MQ=255)

                                                 aaaaCTTTATTTCTGCCTTAGCGGTCGGAAAGATTTCCGAAGATCGGCACACTGACGCTAAGGCCGAGCGTATTTAGGGTAATGCCCCATCACCCCAACGAAGGAGACACGGCAAACGACTCGGTGATCGTTGTTAAagc                                                                                            >  2:7257944/1‑140 (MQ=255)

                                                           ttctGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAaata                                                                                  >  2:4699963/1‑140 (MQ=255)

                                                            tctGCTGTGGCGGTCGCAATGCTTTCCGAGGATCGCGACACTGCCGCGCCGGCGGTGCTTATAGAGGTTATTGCCCCATCCCCCCCAGGCCGGATCAAAGGAAAACGACTCGTTTATCGTTGTTAATGCAGCAGAaataa                                                                                 <  2:1208106/140‑1 (MQ=255)

                                                                           ttaagtcTTCCCGACGATCGGCACACTGACGCTACGGCCGAGCTTATAGTGGGTAATGCCCAATCACCCCCAGCAAGTACAAACGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                  <  1:7233114/134‑1 (MQ=255)

                                                                           gtaatgCTTTCCGACGAGAGGAACCGTGACGCTAAGGCCGAGCGTTTAGCGGGTAATGCCCCATGACCCCCAGTAAGTAGAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                  <  2:1556954/135‑1 (MQ=255)

                                                                           gtaacgCTTTCCGACGATCGGCACACTGACGAAAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                  <  2:2345117/135‑1 (MQ=255)

                                                                           ggAATGATTTCCCACGATCGGCACACGGACGCTACGGCCTAGCGTATAGAGGGTAATGCCCAATGACCCCCAGCAAGGCGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                  <  1:6623493/140‑1 (MQ=255)

                                                                           ggAACGGTTCCCGACGATGGGCACACTGCCGCTTCGGGGGAGGTTTTAGAGGGTAAGGCCCCATGCCCCCCCGGACGCAGAAACGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                  <  2:4162398/140‑1 (MQ=255)

                                                                                   ttCCGACGATCGGCACACTGACGCTAAGGCCGAGCTTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATa                                                          <  2:4650256/140‑1 (MQ=255)

                                                                                    cccGACGATCGGCACAAGGACGCTACGGCCGTGCGTATAGTGGTTAAGGCCCCATCACCCCCAGGAAGGAGACCCGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaa                                                         <  1:1406861/139‑1 (MQ=255)

                                                                                     cctactaTCGGCACACTGACGATAAGGCCGAGCTTATAGCGGTTAATGCCCCAGGACCCCCCGGACGGAGAAACGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaaa                                                        <  2:1040443/134‑1 (MQ=255)

                                                                                     ccGTCGATGGTCACACTTACGATACGGCCTTGCTTATATAGGGAATTGCCCCTTCACCCCCAGGTAGGACAAACGTCAAACGACTCGTTTATAGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaaa                                                        <  2:5652302/140‑1 (MQ=255)

                                                                                          gaggggCACAGTGACGCTAAGGCCGAGGGTATAGCGGTTAATGCCCCTTGCCCCCCAGGACGGACAAACGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                   <  2:3014835/136‑1 (MQ=255)

                                                                                          gaTCGGCACACTCCCCCCACGCCCGAGCGTATCGCGGTTAAGCCCCCAGCCCCCCCCGCATGCACAAACGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                   <  1:1003507/140‑1 (MQ=255)

                                                                                          gaTCGCCACCCTGCCGCTACGGCCGAGCTTTGAGCGGGTAAGCCCCCATCCCCCACTGCACGCACAACCGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                   <  1:155550/140‑1 (MQ=255)

                                                                                           aTCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAGAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACAcc                                                  >  1:4820647/1‑140 (MQ=255)

                                                                                           aTCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACAcc                                                  >  1:4960057/1‑140 (MQ=255)

                                                                                                    cttcctatAAGCCCGAGCTTATAGAGGTTAATGCCCCATGACCCCCAGTATGTCTAAACGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTaaa                                         <  2:4491198/133‑1 (MQ=255)

                                                                                                     tGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                  >  1:376700/1‑114 (MQ=255)

                                                                                                      gACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACa                                       >  1:3719491/1‑140 (MQ=255)

                                                                                                          cTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCt                                   >  2:3764439/1‑140 (MQ=255)

                                                                                                          cTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCt                                   >  2:7180804/1‑140 (MQ=255)

                                                                                                          cTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCt                                   >  2:7684792/1‑140 (MQ=255)

                                                                                                          cTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCt                                   >  1:1195334/1‑140 (MQ=255)

                                                                                                          cTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACGCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCt                                   >  2:6737847/1‑140 (MQ=255)

                                                                                                             aGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGAt                                >  1:1679027/1‑140 (MQ=255)

                                                                                                             aGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGAt                                >  1:4011705/1‑140 (MQ=255)

                                                                                                             aGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGAt                                >  1:6246431/1‑140 (MQ=255)

                                                                                                                 cgtgcttattGAGGGTATTGCCCCCTTCCGCCCTGGAAGCATCATCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTGCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGcc                            <  1:6900876/134‑1 (MQ=255)

                                                                                                                   aGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCa                          >  1:6606545/1‑140 (MQ=255)

                                                                                                                          gacaggTAAGGCCCCATGACCCCCAGGACGGAGAAAGGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTGATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTgg                   <  2:4305650/136‑1 (MQ=255)

                                                                                                                              gttAATGCCCCATTACCCCCAGGACGTATAAACGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt               <  1:1886364/138‑1 (MQ=255)

                                                                                                                              ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATCAACACACCAATCGTAAACAGGCTGATCGCCCTCTTTTGGCttt               >  1:7389681/1‑140 (MQ=255)

                                                                                                                              ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                   >  1:6047146/1‑104 (MQ=255)

                                                                                                                              ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt               >  1:4192857/1‑140 (MQ=255)

                                                                                                                              ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt               >  1:4301135/1‑140 (MQ=255)

                                                                                                                              ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt               >  2:4132154/1‑140 (MQ=255)

                                                                                                                              ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt               >  1:543147/1‑140 (MQ=255)

                                                                                                                              ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt               >  2:3515914/1‑140 (MQ=255)

                                                                                                                              ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt               >  1:3296437/1‑140 (MQ=255)

                                                                                                                              ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt               >  2:5631932/1‑140 (MQ=255)

                                                                                                                              ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt               >  2:6307921/1‑140 (MQ=255)

                                                                                                                              ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt               >  1:2908554/1‑140 (MQ=255)

                                                                                                                              ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCtgt               >  1:7071576/1‑138 (MQ=255)

                                                                                                                              ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGGTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt               >  2:4603715/1‑140 (MQ=255)

                                                                                                                              ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAAATCCGTTATCCAGCGCAGCCATAAACACACCAAACGTAAACAGGCTCATCGCCCACATTTGGCttt               >  2:5762708/1‑140 (MQ=255)

                                                                                                                               ttactcccccATCACCCCCAGGAAGCACAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCtttt              <  1:6111484/134‑1 (MQ=255)

                                                                                                                                 aaTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                  >  1:5626746/1‑86 (MQ=255)

                                                                                                                                 aaTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCt            >  2:5234315/1‑140 (MQ=255)

                                                                                                                                 aaTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCt            >  2:5101824/1‑140 (MQ=255)

                                                                                                                                 aaTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCt            >  2:782747/1‑140 (MQ=255)

                                                                                                                                 aaTGCCCCATGACCCCCAGGAAGCAGAAACGGCAAACGAATCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCCCCCACTGTTGGCTTTTCt            >  1:4520453/1‑140 (MQ=255)

                                                                                                                                  aggCCCCAGGACCCCCAGGAAGTACAAACGGCAAACGACTCGTTTATCGTTGTTAGAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTg           <  2:4820647/138‑1 (MQ=255)

                                                                                                                                  aTGCCCCATTACCCCCAGGAAGGATAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTg           <  2:58951/140‑1 (MQ=255)

                                                                                                                                  aTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTg           >  1:7740802/1‑140 (MQ=255)

                                                                                                                                   tcccccTTGACCCCCCGTAAGGACCACCGCAACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGc          <  1:3118615/138‑1 (MQ=255)

                                                                                                                                   tccccaATGACCCCCAGCAAGCACAACCGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGc          <  1:5214672/138‑1 (MQ=255)

                                                                                                                                   tGCCCCATGACCCCCCGGAAGCAGAAACGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGc          <  2:7379437/140‑1 (MQ=255)

                                                                                                                                   ggCCCCATCCCCCCAAGCACGGACAACCGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATCATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGc          <  1:3577380/139‑1 (MQ=255)

                                                                                                                                    gCCCCATGCCCCCCCGGACGGACAAACGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct         <  1:3042198/140‑1 (MQ=255)

                                                                                                                                    gCCCCATGCCCCCCAGGACGTACAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct         <  2:2762816/140‑1 (MQ=255)

                                                                                                                                    gCCCCATGACCCCCCGGAAGCAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct         <  2:1958814/140‑1 (MQ=255)

                                                                                                                                    gCCCCATCACCCCCCTCAAGGAGACACGCACCACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct         <  2:6465485/140‑1 (MQ=255)

                                                                                                                                    cccccATGACCCCCCGGAAGGACAACCGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct         <  1:974616/139‑1 (MQ=255)

                                                                                                                                     ccacaggACCCCCAGGACCTAGACACGGCAAACGACTCCTTGATCGTTGTTAAAGCAGCAGAACTAATTCCCTGATCGAGCGCAGCCCTAAACACACCAATCGTACAAAGGCGGATCGCCCACTTTTGGCTTTTCGGctc        <  2:7578698/134‑1 (MQ=255)

                                                                                                                                        cATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaaa                                                        >  1:6801504/1‑89 (MQ=255)

                                                                                                                                        cATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaaa                                                        <  2:6801504/89‑1 (MQ=255)

                                                                                                                                        cATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt     >  2:1495562/1‑140 (MQ=255)

                                                                                                                                        cATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt     >  1:1768233/1‑140 (MQ=255)

                                                                                                                                        cATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt     >  2:5077368/1‑140 (MQ=255)

                                                                                                                                        cATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGGTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAAGCGTAAACAGGCTGATCGCCCCCGTTTGGCTTTTCTGCTCTgg     >  2:3858721/1‑139 (MQ=255)

                                                                                                                                          tGACCCCCCGTACGGACAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa   <  2:2599252/140‑1 (MQ=255)

                                                                                                                                          tGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa   >  1:2433301/1‑140 (MQ=255)

                                                                                                                                           gACCCCCCGGAAGGATAAACGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCaa  <  1:6339671/140‑1 (MQ=255)

                                                                                                                                           gACCCCCAGGAAGTACAACCCCCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCaa  <  2:3335298/140‑1 (MQ=255)

CAAAGCAACAAGCAAAGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAA  >  NZ_CP034943/886597‑886875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`