BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	886,747	A→C	8.1%	P47P (CCT→CCG)	EO946_RS04490 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	886,747	0	A	C	8.1%	659.1 / 3.7	301	P47P (CCT→CCG)	EO946_RS04490	MFS transporter
Reads supporting (aligned to +/- strand): ref base A (166/85); new base C (3/38); total (169/132)
Fisher's exact test for biased strand distribution p-value = 3.59e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 309 total aligned reads displayed.

AAAGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACA  >  NZ_CP034943/886610‑886886

aaaGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTCTAGAGGGTAATGCCCCATCACCCCCcggacgg                                                                                                                                           >  2:5303752/1‑140 (MQ=255)

     aCCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGaaa                                                                                                                                      <  2:345599/140‑1 (MQ=255)

     aCCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGaaa                                                                                                                                      <  2:5983470/140‑1 (MQ=255)

         aGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGc                                                                                                                                  >  2:6677184/1‑140 (MQ=255)

         aGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGc                                                                                                                                  >  2:6339671/1‑140 (MQ=255)

              gAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACg                                                                                                                             >  2:7217369/1‑140 (MQ=255)

               aaaagcaaaaCCACAATCAAAAAATGTTATTTTCTGCCTGAGGGGTCGAAACGATTACCGACTATCGGCACACTGACCCTACGGCCGAGCTTATCGCGGGTAATGCCCCATGACCCCCAGCACGGAGAAACGGCAAACGa                                                                                                                            <  2:1434825/133‑1 (MQ=255)

                        cccccAATCACAAAAATTTACTTTCTGCCGTCGGGGTCGCAACGGTTTCCGACGTGCGCAACACGGACGATAAGGCCGAGCGTATAGAGGGAAATGCCCCATCACCCCCAGCACGCACAAACGGCACACGACTCGTTTAt                                                                                                                   <  2:6480193/136‑1 (MQ=255)

                              aTGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGGATAGAGGGTAATGCCCCATGACCCCCAGGCAGGAGAAACGGCAAACGACTCGTTTATCgttgt                                                                                                             >  1:469179/1‑140 (MQ=255)

                                   aaaaaGTTTGTTTGTGCCGTAGGGGTCGTAAAGCTTTCCGACGATCGGCACACTGACGATACGGCCGAGGTTTTTGAGGGTAATGCCCAATGACCCCCAGGAAGCAGAACCGGCAAACGACTCGTTTATCGTTGTTAAag                                                                                                        <  2:4375230/140‑1 (MQ=255)

                                   aaaaaGTTTCTTTCTGCTTTCGCGGTCGGAAAGCTTTCCGACGATCGCCACAATGACGCTAAGGCCGAGCGTATAGAGGGTACTCCCCCATCACCCCACGGACGGAGAACCGCCAAACGACTCGTTTATCGTTGTTAAag                                                                                                        <  2:7317519/140‑1 (MQ=255)

                                    aaaaTTTTTTTTCTGCCTTCGCGGGCTCAAAGTTTTCCCACTATCGGCACACTGACGCTACGGCCGAGCGTATCTCGGGTAATGCCCCATGACCCCCCGCAAGGAGAAAGGGCAAACGACTCGTTTATCGTTGTTAAagc                                                                                                       <  2:2227351/140‑1 (MQ=255)

                                           tctttctGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTAAAGAGGGTAATGCCCCATGACCCCCAGGACGGACAAACGGCAAACGACTCGTTTATCGTTTGTAAAGCAGCAGaa                                                                                                >  2:2081070/1‑140 (MQ=255)

                                               tctGCGGTCGGGTTCGTAATGTTTCCCCACGATCGGCACACTGACGATAAGGCCGAGCTTATAGCGGTTAATGCCCAATGACCCCCAGGAAGGACACACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAaataa                                                                                            <  1:5775578/140‑1 (MQ=255)

                                               tctGCGGTCGGGTTCGAAAAGCTGCCCCACTATCGGCACACTGCCCCTACGGCCGAGGTTATTGAGGGTAATGCCCCATGACCCCCAGGAAGGACAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAaataa                                                                                            <  1:5295643/140‑1 (MQ=255)

                                               tctGCCGTAGGGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGCACGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAaataa                                                                                            <  2:6199032/140‑1 (MQ=255)

                                                      tAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGtt                                                                                     >  2:2495676/1‑140 (MQ=255)

                                                              gtaatgCTTTCCGACGAGAGGAACCGTGACGCTAAGGCCGAGCGTTTAGCGGGTAATGCCCCATGACCCCCAGTAAGTAGAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                             <  2:1556954/135‑1 (MQ=255)

                                                              ggAACGCTTTCCGACGAGCGGCACACTGACGCTAAGGCCGAGCGTTTAGTGGTTAATGCCCCAGGACCCCATGGAAGGATAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                             <  2:3549145/140‑1 (MQ=255)

                                                              gaaattcTTCCCGCCCATCGCCACACTCCCTCCACGGCCGTGCTTATCTTGGGTAATGCCCCATCCCCCCAAGGAAGGACAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                             <  1:6152849/134‑1 (MQ=255)

                                                                atcgCTTCCCGACGATCGGCACACTGCCGCTAAGGCCGAGCGTATAGTGGTTAAGCCCCCATGACCCCCAGGACGGACAACCGCCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCa                                                                           <  1:2808350/137‑1 (MQ=255)

                                                                  cgttttCGGCCGATCGGCACCCGGACGCTACGGCCGCGCTTATAGAGGGTAATCCCCCATGACCCCCCGTCAGGAGAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGc                                                                         <  2:7764679/137‑1 (MQ=255)

                                                                       cccGACGATCGGCACAAGGACGCTACGGCCGTGCGTATAGTGGTTAAGGCCCCATCACCCCCAGGAAGGAGACCCGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaa                                                                    <  1:1406861/139‑1 (MQ=255)

                                                                        cctactaTCGGCACACTGACGATAAGGCCGAGCTTATAGCGGTTAATGCCCCAGGACCCCCCGGACGGAGAAACGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaaa                                                                   <  2:1040443/134‑1 (MQ=255)

                                                                        ccGTCGATGGTCACACTTACGATACGGCCTTGCTTATATAGGGAATTGCCCCTTCACCCCCAGGTAGGACAAACGTCAAACGACTCGTTTATAGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaaa                                                                   <  2:5652302/140‑1 (MQ=255)

                                                                        ccGAGGTTCGGCACACTTCCGATAAGGCCGAGGTTATATTGGGTAATGCCCCATCACCCCCAGGAAGGATAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaaa                                                                   <  1:4276239/140‑1 (MQ=255)

                                                                             tttCGGCACACTGACGCAACGGCCGAGCTTATTGAGGGTAATCCCCCATGACCCCCAGGAAGGACAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                              <  2:4692028/138‑1 (MQ=255)

                                                                             gaTCGGCACACTCCCCCCACGCCCGAGCGTATCGCGGTTAAGCCCCCAGCCCCCCCCGCATGCACAAACGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                              <  1:1003507/140‑1 (MQ=255)

                                                                             gaTCGCCACCCTGCCGCTACGGCCGAGCTTTGAGCGGGTAAGCCCCCATCCCCCACTGCACGCACAACCGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                              <  1:155550/140‑1 (MQ=255)

                                                                              ttgggCACAGTGTCGGTAAGGCGGAGCGTATAGCGGTTAAGGCCTCCTGACCCCCAGCACGCAGAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACAcc                                                             <  2:1203723/137‑1 (MQ=255)

                                                                              aTCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACAcc                                                             >  1:6798143/1‑140 (MQ=255)

                                                                                gggCACACTGCCGCCACGGCGGAGCTTATTGTGGGTTTTCCCCATTGCCCCCCCGGATGTCCAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCaa                                                           <  1:2562895/139‑1 (MQ=255)

                                                                                 ggCAAAGTGACGCTAAGGCCGAGCTTATCGAGGGTAAGGCCCAATGACCCCCAGGACGGAGAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAAt                                                          <  1:3766437/140‑1 (MQ=255)

                                                                                     aacTGACGCTAAGGCCGAGCGTATAGCGGTTAATCCCCCTTGCCCCCCAGGAAGCACAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTa                                                      <  1:6319409/139‑1 (MQ=255)

                                                                                       cttcctatAAGCCCGAGCTTATAGAGGTTAATGCCCCATGACCCCCAGTATGTCTAAACGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTaaa                                                    <  2:4491198/133‑1 (MQ=255)

                                                                                       cgtccGCTATGGCCGAGCGTTTAGCGGTTAAGGCCCAATGACCCCCAGCAAGTAGAAAGGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTaaa                                                    <  2:2406371/136‑1 (MQ=255)

                                                                                       cTGACTATAAGGCCGAGCTTATAGAGGTTAATGCCCAATGACCCCAAGTAAGGATAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTaaa                                                    <  2:4217512/140‑1 (MQ=255)

                                                                                        tGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                             <  2:376700/114‑1 (MQ=255)

                                                                                        tGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                             >  1:7402169/1‑114 (MQ=255)

                                                                                        tGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAAc                                                   >  2:7015758/1‑140 (MQ=255)

                                                                                        tGACGATAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                             <  2:7402169/114‑1 (MQ=255)

                                                                                         gACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACa                                                  >  1:657378/1‑140 (MQ=255)

                                                                                         gACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACa                                                  >  1:3719024/1‑140 (MQ=255)

                                                                                         gACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACa                                                  >  1:3156270/1‑140 (MQ=255)

                                                                                             cTAAGGCCGAGCGTATAGAGGGTACTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCt                                              >  1:3196909/1‑140 (MQ=255)

                                                                                             cTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCt                                              >  2:4818148/1‑140 (MQ=255)

                                                                                             cTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCt                                              >  1:1195334/1‑140 (MQ=255)

                                                                                             cTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCt                                              >  1:5518525/1‑140 (MQ=255)

                                                                                                aGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGAt                                           >  1:1679027/1‑140 (MQ=255)

                                                                                                      aGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCa                                     >  1:2170902/1‑140 (MQ=255)

                                                                                                            taGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCCCTTTTg                               >  1:5966698/1‑140 (MQ=255)

                                                                                                            taGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTg                               >  1:6892112/1‑140 (MQ=255)

                                                                                                            taGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTg                               >  2:311132/1‑140 (MQ=255)

                                                                                                                 gttAATGCCCCATTACCCCCAGGACGTATAAACGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                          <  1:1886364/138‑1 (MQ=255)

                                                                                                                 ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                          >  2:3408558/1‑140 (MQ=255)

                                                                                                                 ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                          >  2:4141167/1‑140 (MQ=255)

                                                                                                                 ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                          >  2:4897950/1‑140 (MQ=255)

                                                                                                                 ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                          >  2:4603729/1‑140 (MQ=255)

                                                                                                                 ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                          >  1:2911674/1‑140 (MQ=255)

                                                                                                                 ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                          >  1:6620455/1‑140 (MQ=255)

                                                                                                                 ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                          >  1:3296437/1‑140 (MQ=255)

                                                                                                                 ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                          >  2:5631932/1‑140 (MQ=255)

                                                                                                                 ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTGTGGCttt                          >  1:3896715/1‑140 (MQ=255)

                                                                                                                 ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAAATCCGTTATCCAGCGCAGCCATAAACACACCAAACGTAAACAGGCTCATCGCCCACATTTGGCttt                          >  2:5762708/1‑140 (MQ=255)

                                                                                                                 ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACGCGTTTATCGTTGTTAAAGCAGCAGAAAGAATTCCCTTATCCAGCGCAGCCAGAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGTCttt                          >  2:1817154/1‑140 (MQ=255)

                                                                                                                    aaTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCa                                                                           >  1:4133379/1‑88 (MQ=255)

                                                                                                                    aaTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCt                       >  2:5234315/1‑140 (MQ=255)

                                                                                                                    aaTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCt                       >  1:6458814/1‑140 (MQ=255)

                                                                                                                    aaTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCt                       >  2:5101824/1‑140 (MQ=255)

                                                                                                                    aaTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCt                       >  2:4130369/1‑140 (MQ=255)

                                                                                                                    aaTGCCCCATGACCCCCAGGAAGCAGAAACGGCAAACGAATCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCCCCCACTGTTGGCTTTTCt                       >  1:4520453/1‑140 (MQ=255)

                                                                                                                     aTGCCCCATGACCCCCAGTAAGTACAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTg                      <  2:6798143/140‑1 (MQ=255)

                                                                                                                     aTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTg                      >  1:7740802/1‑140 (MQ=255)

                                                                                                                     aTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTGTTGGCTTTTCTg                      >  1:4840617/1‑140 (MQ=255)

                                                                                                                      tGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGc                     >  1:2296659/1‑140 (MQ=255)

                                                                                                                      ggCCCCATGACACCAAGGATGGACAAACGCAACAAGACTCTTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGc                     <  1:7217369/139‑1 (MQ=255)

                                                                                                                      ggCCCCATCCCCCCAAGCACGGACAACCGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATCATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGc                     <  1:3577380/139‑1 (MQ=255)

                                                                                                                       gCCCCATGCCCCCCAGGACGTACAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct                    <  2:2762816/140‑1 (MQ=255)

                                                                                                                       gCCCCATGACCCCCCGGAAGCAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct                    <  2:1958814/140‑1 (MQ=255)

                                                                                                                       cccccTTGACCCCCTGAATGTAGAACGGTCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAAGTCCGGTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct                    <  1:4323805/139‑1 (MQ=255)

                                                                                                                       cccccATGACCCCCCGGAAGGACAACCGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct                    <  1:974616/139‑1 (MQ=255)

                                                                                                                       cccccATGACCCCCAGGAAGGACAAACGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct                    <  1:7012823/139‑1 (MQ=255)

                                                                                                                        ccacaggACCCCCAGGACCTAGACACGGCAAACGACTCCTTGATCGTTGTTAAAGCAGCAGAACTAATTCCCTGATCGAGCGCAGCCCTAAACACACCAATCGTACAAAGGCGGATCGCCCACTTTTGGCTTTTCGGctc                   <  2:7578698/134‑1 (MQ=255)

                                                                                                                         cccATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGctct                  >  1:7251796/1‑140 (MQ=255)

                                                                                                                         cccATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGctct                  >  1:3059701/1‑140 (MQ=255)

                                                                                                                           cATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                >  2:5883056/1‑140 (MQ=255)

                                                                                                                           cATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                >  1:1768233/1‑140 (MQ=255)

                                                                                                                           cATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                >  2:5077368/1‑140 (MQ=255)

                                                                                                                           cATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                >  1:2503213/1‑140 (MQ=255)

                                                                                                                             tgccccccATGACTGAGAAACGCCACACGAATCGTTTATCGTTGTTAAACCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa              <  2:2115007/137‑1 (MQ=255)

                                                                                                                             tGACCCCCCGTACGGACAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa              <  2:2599252/140‑1 (MQ=255)

                                                                                                                             tGACCCCCAGGAAGGAGAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa              >  1:1996858/1‑140 (MQ=255)

                                                                                                                             tGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa              >  2:7681867/1‑140 (MQ=255)

                                                                                                                             tGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa              >  1:5381661/1‑140 (MQ=255)

                                                                                                                             tGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa              >  2:3632117/1‑140 (MQ=255)

                                                                                                                                 ccccAGGAAGGAGACACGGCAAACGACTCGTTTATCGTTGTTACAGCAGCAGAAATAATTCGGTTATCCAGCGCTGCCATACTCACTCCTCTCGTAAACCGGCTGTTCGCCCCCTTGTGGCTTTTCTGCTCTGTCAATGc          >  2:6802956/1‑140 (MQ=255)

                                                                                                                                   ccAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCtt        >  2:4084855/1‑140 (MQ=255)

                                                                                                                                   ccAGCAAGGAGAACGGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCtt        <  2:2891436/140‑1 (MQ=255)

                                                                                                                                     aggaaggaGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCa      <  1:5077368/140‑1 (MQ=255)

                                                                                                                                       gaaggaGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGa    >  2:3935331/1‑140 (MQ=255)

AAAGAACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACA  >  NZ_CP034943/886610‑886886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`