BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	886,758	C→A	5.7%	A44S (GCC→TCC)	EO946_RS04490 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	886,758	0	C	A	5.7%	802.7 / 5.7	316	A44S (GCC→TCC)	EO946_RS04490	MFS transporter
Reads supporting (aligned to +/- strand): ref base C (171/115); new base A (2/28); total (173/143)
Fisher's exact test for biased strand distribution p-value = 1.16e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 321 total aligned reads displayed.

AGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACATAT  >  NZ_CP034943/886619‑886889

aGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGACGGAGAAACGGc                                                                                                                                     >  2:5126424/1‑140 (MQ=255)

aGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGGATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACCGc                                                                                                                                     >  1:3195060/1‑140 (MQ=255)

     gAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACg                                                                                                                                >  2:7217369/1‑140 (MQ=255)

                ccTAAATCACAAAAATTTTCTTTCTGCCGTAGCGGTGGGAAAGCTTTCCGACGATCGGCACACGGACGCTAAGGCCGAGCTTATAGCGGGTAATCCCCCAGGACCCCCAGGAAGCACAACCGGAAAACGACTCGTTTATc                                                                                                                     <  2:2197081/140‑1 (MQ=255)

                     ctGATAAAACTTTTCTTTCTGCCGTCGCGTTCGCAACGCTTTCCGACGATCGGCACACTGACGATACGGCCGAGCGTATAGAGGGTAAGGCCCCATCACCCCCTGGACGCACAAACGGCAAACGACTCGTTTATCgttgt                                                                                                                <  2:2602120/139‑1 (MQ=255)

                     aTGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCgttgt                                                                                                                >  2:3042198/1‑140 (MQ=255)

                          aaaaaGTTTCTTTCTGCTTTCGCGGTCGGAAAGCTTTCCGACGATCGCCACAATGACGCTAAGGCCGAGCGTATAGAGGGTACTCCCCCATCACCCCACGGACGGAGAACCGCCAAACGACTCGTTTATCGTTGTTAAag                                                                                                           <  2:7317519/140‑1 (MQ=255)

                           acatgtttgtttGTGGCGTCGCGGGGGGAAAGGTTTCGGACGATGGGAACAATTCCGCTATGGCCGAGGGTTTAGTGGGTAAGGCCCCTTGACCCCCAGGCAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAagc                                                                                                          <  2:1667855/136‑1 (MQ=255)

                           aagaGTTTTTTTCTGCCGTAGCGGTCGGAAAGTTTTCCGACGATTGGCACACTGACGCTAAGACCGAGCGTATAAAGGGTAATGCCCCATGATCCCCAGGAAGGGGAAACGGAAAACGACTCGTTTATCGTTGTTAAagc                                                                                                          >  2:2852220/4‑140 (MQ=255)

                           aaaaGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGATCGTATAGAGGGTAATCCCCCATGACCCCCAGGAAGGAGAAACGGCAAACTACTCGTTTATCGTTGTTAAaga                                                                                                          >  2:2295830/1‑139 (MQ=255)

                                  tctttctGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGaa                                                                                                   >  2:2563777/1‑140 (MQ=255)

                                     ttctGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAaata                                                                                                >  2:4699963/1‑140 (MQ=255)

                                      tctGCTGTGGCGGTCGCAATGCTTTCCGAGGATCGCGACACTGCCGCGCCGGCGGTGCTTATAGAGGTTATTGCCCCATCCCCCCCAGGCCGGATCAAAGGAAAACGACTCGTTTATCGTTGTTAATGCAGCAGAaataa                                                                                               <  2:1208106/140‑1 (MQ=255)

                                           tgtcggggTCGCAAAGCTTTCCGACTATCGGAACACTGACGCTACGGCCGCGGGAATAGCGGTTAATGCCCCATCCCCCCCAGTATGGACAAACGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCg                                                                                          <  1:1993936/134‑1 (MQ=255)

                                           cttcgCGGTCTTAAAGCTAACCTCCGATCGGAACACGCACGCTAAGGCCGAGCTTATAGAGTTTAATCCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCg                                                                                          <  1:5296573/136‑1 (MQ=255)

                                                     gtaaaGCTTTCCCACCATCGGCAAACTTACGCTAAGGCCGAGCTTTTAGTTGTTAATGCCCCATGACCCCCTGTAAGGATAAACGGAAAACGACTCGTTTATCGTTGTTAAATAATCATAAATAATTCCGTTATCCAgag                                                                                <  2:2106628/138‑3 (MQ=255)

                                                     ggAACGCTTTCCGACGAGCGGCACACTGACGCTAAGGCCGAGCGTTTAGTGGTTAATGCCCCAGGACCCCATGGAAGGATAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                <  2:3549145/140‑1 (MQ=255)

                                                         cgttttCGGCCGATCGGCACCCGGACGCTACGGCCGCGCTTATAGAGGGTAATCCCCCATGACCCCCCGTCAGGAGAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGc                                                                            <  2:7764679/137‑1 (MQ=255)

                                                         agatttCCGACGATGGGCACACTGCGGCTAAGGCCCAGCGTATAGTGGGTAATGCCCCATTCCCCCCATGAAGCACAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGc                                                                            <  2:5260159/137‑1 (MQ=255)

                                                          gtttgccGACGATCGGCACCCTCACGCTAAGGCCGTGCTTATAGCGGGTATTGCCCCATGACCCCCAGGAAGGAGAACGGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGcc                                                                           <  1:5799657/135‑1 (MQ=255)

                                                              cccGACGATCGGCACAAGGACGCTACGGCCGTGCGTATAGTGGTTAAGGCCCCATCACCCCCAGGAAGGAGACCCGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaa                                                                       <  1:1406861/139‑1 (MQ=255)

                                                               cctaggaTCGCCACACTGACGCTACGGCCGAGCGTATAGAGGTTAATGCCCCATCACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaaa                                                                      <  2:6475876/135‑1 (MQ=255)

                                                               ccGTCGATGGTCACACTTACGATACGGCCTTGCTTATATAGGGAATTGCCCCTTCACCCCCAGGTAGGACAAACGTCAAACGACTCGTTTATAGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaaa                                                                      <  2:5652302/140‑1 (MQ=255)

                                                               ccGACGATGGGAACACTGACGCTACGGCCGAGCGTTTTGTGGGTAAGGCCCAATGACCCCCTGGAAGGATAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaaa                                                                      <  2:1882098/140‑1 (MQ=255)

                                                               ccGACGATGGCCACACTTCCCCTACGGCCTAGGTTATAGTGGGTAATCCCCCATCACCCCCAGTAAGGATAAACGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaaa                                                                      <  2:1132527/140‑1 (MQ=255)

                                                               ccGACGATCGGCACACTCCCGCTACGGCCGAGCGTATAGAGGGTAAGCCCCCATCCCCCCCAGGAAGGACACACGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaaa                                                                      <  1:2788333/140‑1 (MQ=255)

                                                                    gttCGGCAAACTGACGATAAGGCCGTGGGTATAGAGGTTAATGCCCAATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                 <  2:2409794/138‑1 (MQ=255)

                                                                    gctCGCCACACTGACGCTACGGCCGAGCGTATCGTGGGTAAGGCCCCAGCACCCCCAGGACGGAGAAAGGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                 <  2:5090982/138‑1 (MQ=255)

                                                                    gaTCGGCACAGTGACGATACGGCCGAGGTTTTTGTGGGTATTGCCCCTTGACCCCCAGGAAGGATAAAGGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                 <  1:4760628/140‑1 (MQ=255)

                                                                    gaTCGGCACACTCCCCCCACGCCCGAGCGTATCGCGGTTAAGCCCCCAGCCCCCCCCGCATGCACAAACGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                 <  1:1003507/140‑1 (MQ=255)

                                                                    gaTCGGAACACTGACGCTAAGGCCGAGCTTATAGAGGGTAATCCCCCATCACCCCCAGGAAGGAGAACCGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                 <  1:6674865/140‑1 (MQ=255)

                                                                    gaTCGCCACCCTGCCGCTACGGCCGAGCTTTGAGCGGGTAAGCCCCCATCCCCCACTGCACGCACAACCGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                 <  1:155550/140‑1 (MQ=255)

                                                                     aTCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACAcc                                                                >  1:4960057/1‑140 (MQ=255)

                                                                     aTCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGGTATCCAGCGCAGCCATAAACACAcc                                                                >  1:97591/1‑140 (MQ=255)

                                                                       gggCACACTGCCGCCACGGCGGAGCTTATTGTGGGTTTTCCCCATTGCCCCCCCGGATGTCCAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCaa                                                              <  1:2562895/139‑1 (MQ=255)

                                                                        ggCACACTGAGGCTAAGGCCGAGCTTTTTGTGTGTAATGCCCATTGCCCCCCAGCAAGGTGAAAGGGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAAt                                                             <  1:6473288/140‑1 (MQ=255)

                                                                        ggCAAAGTGACGCTAAGGCCGAGCTTATCGAGGGTAAGGCCCAATGACCCCCAGGACGGAGAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAAt                                                             <  1:3766437/140‑1 (MQ=255)

                                                                               tGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAAc                                                      >  2:7015758/1‑140 (MQ=255)

                                                                                gACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACa                                                     >  1:3156270/1‑140 (MQ=255)

                                                                                    cTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCACTCGTCAACAGGCt                                                 >  1:4212294/1‑140 (MQ=255)

                                                                                    cTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCt                                                 >  2:4852391/1‑140 (MQ=255)

                                                                                    cTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCt                                                 >  1:1195334/1‑140 (MQ=255)

                                                                                       aGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGAt                                              >  1:1679027/1‑140 (MQ=255)

                                                                                             aGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCa                                        >  1:6606551/1‑140 (MQ=255)

                                                                                             aGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCa                                        >  1:2170902/1‑140 (MQ=255)

                                                                                                   taGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCCCTTTTg                                  >  1:5966698/1‑140 (MQ=255)

                                                                                                   taGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTg                                  >  1:6892112/1‑140 (MQ=255)

                                                                                                    gacaggTAAGGCCCCATGACCCCCAGGACGGAGAAAGGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTGATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTgg                                 <  2:4305650/136‑1 (MQ=255)

                                                                                                    agcgggTAAGCCCCCATGACCCCCAGGAAGGACAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTgg                                 <  2:1992261/137‑1 (MQ=255)

                                                                                                       gggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCtt                              >  1:186541/1‑140 (MQ=255)

                                                                                                        ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATCAACACACCAATCGTAAACAGGCTGATCGCCCTCTTTTGGCttt                             >  1:7389681/1‑140 (MQ=255)

                                                                                                        ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  1:7219499/1‑140 (MQ=255)

                                                                                                        ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  2:4141167/1‑140 (MQ=255)

                                                                                                        ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  2:3515722/1‑140 (MQ=255)

                                                                                                        ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  1:543147/1‑140 (MQ=255)

                                                                                                        ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  1:2908554/1‑140 (MQ=255)

                                                                                                        ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  2:5631932/1‑140 (MQ=255)

                                                                                                        ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  2:4935005/1‑140 (MQ=255)

                                                                                                        ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCtgt                             >  1:7071576/1‑138 (MQ=255)

                                                                                                        ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTGTGGCttt                             >  1:3896715/1‑140 (MQ=255)

                                                                                                        ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAAATCCGTTATCCAGCGCAGCCATAAACACACCAAACGTAAACAGGCTCATCGCCCACATTTGGCttt                             >  2:5762708/1‑140 (MQ=255)

                                                                                                         ttactcccccATCACCCCCAGGAAGCACAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCtttt                            <  1:6111484/134‑1 (MQ=255)

                                                                                                          tAATGCCCCATGACCCCCAGGACGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTGTTGGCTTTTc                           >  2:6562058/1‑140 (MQ=255)

                                                                                                           aaTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCa                                                                              >  1:4133379/1‑88 (MQ=255)

                                                                                                           aaTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCt                          >  2:460693/1‑140 (MQ=255)

                                                                                                           aaTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCt                          >  1:3669801/1‑140 (MQ=255)

                                                                                                           aaTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCt                          >  2:4130369/1‑140 (MQ=255)

                                                                                                            aTGCCCCATGACCCCCAGTAAGTACAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTg                         <  2:6798143/140‑1 (MQ=255)

                                                                                                            aTGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTg                         >  1:7740802/1‑140 (MQ=255)

                                                                                                              gCCCCATGCCCCCCCGGACGGACAAACGCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct                       <  1:3042198/140‑1 (MQ=255)

                                                                                                              gCCCCATCACCCCCATCAAGTACAAAGGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct                       <  2:6025812/140‑1 (MQ=255)

                                                                                                              cccccTTGACCCCCTGAATGTAGAACGGTCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAAGTCCGGTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct                       <  1:4323805/139‑1 (MQ=255)

                                                                                                               ccacaggACCCCCAGGACCTAGACACGGCAAACGACTCCTTGATCGTTGTTAAAGCAGCAGAACTAATTCCCTGATCGAGCGCAGCCCTAAACACACCAATCGTACAAAGGCGGATCGCCCACTTTTGGCTTTTCGGctc                      <  2:7578698/134‑1 (MQ=255)

                                                                                                                  cATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaaa                                                                      >  1:6801504/1‑89 (MQ=255)

                                                                                                                  cATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                   >  2:5883056/1‑140 (MQ=255)

                                                                                                                  cATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                   >  1:2503213/1‑140 (MQ=255)

                                                                                                                  cATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                   >  2:1495562/1‑140 (MQ=255)

                                                                                                                    tGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCCCTTTTGGCTTTTCTGCTCTGTCa                 >  2:380653/1‑140 (MQ=255)

                                                                                                                    tGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa                 >  2:3632117/1‑140 (MQ=255)

                                                                                                                    tGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa                 <  1:5580250/140‑1 (MQ=255)

                                                                                                                    tGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa                 >  2:7681867/1‑140 (MQ=255)

                                                                                                                    tGACCCCCAGCACGGACAACCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa                 <  1:6285760/140‑1 (MQ=255)

                                                                                                                     gACCCCCAGGAAGTACAACCCCCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCaa                <  2:3335298/140‑1 (MQ=255)

                                                                                                                        ccccAGGAAGGAGACACGGCAAACGACTCGTTTATCGTTGTTACAGCAGCAGAAATAATTCGGTTATCCAGCGCTGCCATACTCACTCCTCTCGTAAACCGGCTGTTCGCCCCCTTGTGGCTTTTCTGCTCTGTCAATGc             >  2:6802956/1‑140 (MQ=255)

                                                                                                                        ccccAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGc             >  1:4468699/1‑140 (MQ=255)

                                                                                                                         cccAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                 >  1:7431856/1‑87 (MQ=255)

                                                                                                                          ccAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCtt           >  1:2288719/1‑140 (MQ=255)

                                                                                                                          ccAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCtt           >  2:6278047/1‑140 (MQ=255)

                                                                                                                          ccAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCtt           >  1:3282879/1‑140 (MQ=255)

                                                                                                                          ccAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCtt           >  2:4084855/1‑140 (MQ=255)

                                                                                                                             ggaaggaGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAg        <  2:4468699/140‑1 (MQ=255)

                                                                                                                              gaaggaGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGa       >  2:3935331/1‑140 (MQ=255)

                                                                                                                                aggaGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACa     <  1:460693/140‑1 (MQ=255)

                                                                                                                                  gataaaCGTCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACata   <  1:5126424/137‑1 (MQ=255)

                                                                                                                                  gagaAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACata   >  2:7413833/1‑140 (MQ=255)

                                                                                                                                  gagaAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACata   >  1:1747755/1‑140 (MQ=255)

                                                                                                                                   agaAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAACTAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACatat  >  2:4593555/1‑140 (MQ=255)

                                                                                                                                   agaAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                 >  2:6363525/1‑77 (MQ=255)

                                                                                                                                   agaAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                 <  1:6363525/77‑1 (MQ=255)

                                                                                                                                     aaaCGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCa                                                                              <  1:353666/62‑1 (MQ=255)

AGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACATAT  >  NZ_CP034943/886619‑886889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`