Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 986,786 C→A 6.8% Q367K (CAA→AAA)  spoVR → stage V sporulation protein SpoVR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943986,7860CA 6.8% 1024.8 / 15.8 377Q367K (CAA→AAA) spoVRstage V sporulation protein SpoVR
Reads supporting (aligned to +/- strand):  ref base C (155/183);  new base A (2/37);  total (157/220)
Fisher's exact test for biased strand distribution p-value = 1.56e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 385 total aligned reads displayed.

AGAAATGAAGAAAATGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCT  >  NZ_CP034943/986647‑986924
                                                                                                                                           |                                                                                                                                          
agaaATGAAGAAAATGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTc                                                                                                                                            <  1:90731/140‑1 (MQ=255)
    aTGAAGAAAATGGGCGTGAATAATTTATAATACAGCGAGAAAATGTTTGAGCTAAAACAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAATACTTAGTTATGCTCGAGGATCTTTATTTGTTTAaaaa                                                                                                                                        >  2:4828785/1‑140 (MQ=255)
     tGAAGAACAGGGGCGTGAAGCCTGGCTCAGGCAGGGAGAACATGTTTGAGGTCAGGGAACTTGAATCCGATATCTCTGTCATCCGCACTTACTTAACCCGAGACTGAGTTAGGCGCGGGGATCGTTATTGGGTTCaacag                                                                                                                                       >  2:7399192/1‑137 (MQ=255)
     tGAAGAAAATGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCaaaaa                                                                                                                                       <  2:2771421/140‑1 (MQ=255)
       aagaaAATGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACa                                                                                                                                     <  1:7075446/140‑1 (MQ=255)
       aagaaAATGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACa                                                                                                                                     <  1:4655952/140‑1 (MQ=255)
            aaTGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCa                                                                                                                                <  1:2716389/140‑1 (MQ=255)
             ctGGGCGTGAAGCCTGGCTCAGGCAGGGAGACAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAACAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCag                                                                                                                               <  1:6033993/139‑1 (MQ=255)
              tGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCaga                                                                                                                              <  1:1186561/140‑1 (MQ=255)
                 gCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGAt                                                                                                                           >  2:3256104/1‑140 (MQ=255)
                    tGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTCTGCGCGAGGTTCTTTATTTGTTTCAACAACAGGGCAGAGCTtat                                                                                                                        >  1:5966888/1‑140 (MQ=255)
                    tGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATtat                                                                                                                        >  1:6724262/1‑140 (MQ=255)
                         ccTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAAt                                                                                                                   >  2:6256326/1‑140 (MQ=255)
                           tGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAAtcg                                                                                                                 >  1:2149106/1‑140 (MQ=255)
                           tGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAAtcg                                                                                                                 >  2:6845164/1‑140 (MQ=255)
                                 aggcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACa                                                                                                           <  1:5087182/140‑1 (MQ=255)
                                 aggcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACa                                                                                                           <  1:4935113/140‑1 (MQ=255)
                                 aggcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCGTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACa                                                                                                           <  1:1933726/140‑1 (MQ=255)
                                  ggcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACaa                                                                                                          >  1:2386165/1‑140 (MQ=255)
                                  ggcaggGAGAAAATGTTGGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGAGTATAAAATCGTCGACaa                                                                                                          >  1:5048337/1‑140 (MQ=255)
                                   gcaggGAGAACATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACaaa                                                                                                         >  1:2910031/1‑140 (MQ=255)
                                   gcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCaga                                                                                                                              <  1:2466169/119‑1 (MQ=255)
                                   gcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACaaa                                                                                                         >  1:279650/1‑140 (MQ=255)
                                   gcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACaaa                                                                                                         >  1:1876995/1‑140 (MQ=255)
                                   gcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACaaa                                                                                                         >  1:4542453/1‑140 (MQ=255)
                                       ggAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAaataat                                                                                                     >  1:5224583/1‑140 (MQ=255)
                                       ggAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAAt                                                                                                     >  1:4416653/1‑140 (MQ=255)
                                       ggAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAAt                                                                                                     >  2:5787868/1‑140 (MQ=255)
                                       ggAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAAt                                                                                                     >  1:1005307/1‑140 (MQ=255)
                                        gagaAAATGTTTGAGGTCAGGGAAATTGGATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCaaaaa                                                                                                                                       <  2:1249528/105‑1 (MQ=255)
                                             aaTGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAAt                                                                                               >  1:2195625/1‑140 (MQ=255)
                                                 tttGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGAGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGt                                                                                           >  1:3698672/1‑140 (MQ=255)
                                                  ttGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGACATCGGTa                                                                                          >  2:3408281/1‑140 (MQ=255)
                                                        tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                    <  1:5537045/140‑1 (MQ=255)
                                                        tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                    <  2:5469377/140‑1 (MQ=255)
                                                        tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                    <  2:5690391/140‑1 (MQ=255)
                                                        tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACACGAc                                                                                    <  1:827350/140‑1 (MQ=255)
                                                           gggAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGa                                                                                                       <  2:2083771/118‑1 (MQ=255)
                                                           gggAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAGCAg                                                                                 >  1:608795/1‑140 (MQ=255)
                                                                   gAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAg                                                                         <  1:5802667/140‑1 (MQ=255)
                                                                     aTCCGATATCTCTTTCATCCGCAATGACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAACATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCa                                                                       <  1:1328164/140‑1 (MQ=255)
                                                                      tCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                    <  2:6921806/126‑1 (MQ=255)
                                                                       ccGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATg                                                                     <  2:7162814/140‑1 (MQ=255)
                                                                          atatCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCgg                                                                  >  2:6597746/1‑140 (MQ=255)
                                                                           tatCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCggg                                                                 <  2:3227828/140‑1 (MQ=255)
                                                                                      tCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGc                                                                                >  2:383784/1‑114 (MQ=255)
                                                                                      tCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCggg                                                      >  2:6369944/1‑140 (MQ=255)
                                                                                       ccGCAATTATTTAACCAAAGAATTAGTCATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCCGAGATTATAAAATCATCGATAAACAGTGGAAGTCGGTGCGCGACCAGCTCGTAAGCATGCGGGTAAAtggcggtt                                                     >  2:6912241/1‑138 (MQ=255)
                                                                                       ccGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGt                                                     <  1:5750627/140‑1 (MQ=255)
                                                                                       ccGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGt                                                     >  1:4842552/1‑140 (MQ=255)
                                                                                         gCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGttt                                                   <  2:6447126/140‑1 (MQ=255)
                                                                                           aaTTACTTAACACAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTcc                                                 <  2:4021018/140‑1 (MQ=255)
                                                                                                 ttAACAAAAGACTTAGTTATGCGCGAGGATGTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCatatgt                                           >  2:5172159/1‑138 (MQ=255)
                                                                                                   aaaaaaaGACTTAGTTATGCGCGAGGATCTTTTTTTTTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGa                                         <  1:1595539/137‑1 (MQ=255)
                                                                                                   aaCAAAAGACTTAGTTATGCGCGAGTATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGACTGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGTGTTTCCATATTTGa                                         <  2:4419983/140‑1 (MQ=255)
                                                                                                   aaCAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGa                                         >  1:6228473/1‑140 (MQ=255)
                                                                                                   aaCAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGa                                         >  1:7636010/1‑140 (MQ=255)
                                                                                                     cAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAATTCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACa                                       <  2:5342098/140‑1 (MQ=255)
                                                                                                     cAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACa                                       <  2:1606384/140‑1 (MQ=255)
                                                                                                     cAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACa                                       >  1:7694802/1‑140 (MQ=255)
                                                                                                     cAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACa                                       <  2:2911199/140‑1 (MQ=255)
                                                                                                               aGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAacgacg                             >  2:263989/1‑140 (MQ=255)
                                                                                                               aGTTAGGCGCGTGTATCTTTATTTGTTAAAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGATTGCAAATCGTTACGCGACCAGCTCGTGAGCTTGGGGTTAAACGGCGGGTTTCCTTATTTGACAGTAAacgacg                             <  1:5334786/140‑1 (MQ=255)
                                                                                                                    tGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTCAACGGCGGGTTTCCATATTTGACAGTCAACGACGGTGag                        >  1:4466492/1‑139 (MQ=255)
                                                                                                                           ggATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGa                 >  2:417925/1‑140 (MQ=255)
                                                                                                                                 ttATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATa           >  2:7707736/1‑140 (MQ=255)
                                                                                                                                   tttttttTCAAAAACAGGGCAGATTTTATAAAATGGTCGACAAAGAATGGAAATCGGTACGCGCCCAGCTCGTGAGCATGCGGGTAAACGGGGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATGAc         <  1:7365078/139‑1 (MQ=255)
                                                                                                                                   tttttttTAAAAAACCGGCCAGATATTATAAAATCGTCGACAAATAATGCAAATCGTTACGCGACCAGCTCGTGAGCATGCGGTTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc         <  1:6475963/139‑1 (MQ=255)
                                                                                                                                   tttgttttCAAAAACAGGCCAGAGTTTATAAAATCGTCGAAAAAGAAGGGAAAGCGGTACGCGACCTGCTCGTGAGCATGCGGGTAAACGGCGGTTTTCCATATTTGACTGTAAACGACGGTGATTACATGAAAAATAAc         <  2:1101351/135‑1 (MQ=255)
                                                                                                                                   aTTTTTTTCAAAAACAGGGCAGATATTATAAAATCGTCTAAAAATAATGGAAATCGGTACGCGACCAGCTCGTGAGAATGCGGGTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc         <  2:7553822/140‑1 (MQ=255)
                                                                                                                                   aTTTTTTTCAAAAACAGGGCAGAGATAATAAAATCGTCGACAAAGAATGGAAATCGGTCCGCGACCAGCTCGTGAGCATGGGGGTAAACGGCGGGTTTCCATATTTGACATTAAACGACGGTGATTACATGAAAAATAAc         <  2:4969177/140‑1 (MQ=255)
                                                                                                                                   aTTTTTTTAAAAAACAGGGCAGATTTTATAAAATCGTCGACAAAGATGGGAAATCGGTACGCGCCCAGCTCGTGAGCTTGCGGTTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc         <  1:6959776/140‑1 (MQ=255)
                                                                                                                                   aTTTGTTTCAAAAACAGGGCAGATATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGATTGCGGGTAAACGGGGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc         <  2:3518476/140‑1 (MQ=255)
                                                                                                                                   aTTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc         <  2:2282970/140‑1 (MQ=255)
                                                                                                                                   aTTTGTTTCAAAAACAGGCCAGAGATTATAAAATCGTCGAAAAATAATGGAAATGGGTACGCGACCAGCTCGTGAGCATGGGGGTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc         <  2:3113563/140‑1 (MQ=255)
                                                                                                                                   aTTTGTTTAAAAACCAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAAGCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc         <  2:469187/140‑1 (MQ=255)
                                                                                                                                   aTTTGTTTAAAAAACAGGGCAGATTTTATAAAATCGTCGAAAAAGAATGGAAATCGTTACGCGACCCGCTCTTGAGCATGCGGGTAAACGGCGGTTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc         <  2:6746635/140‑1 (MQ=255)
                                                                                                                                   aTTTGTTTAAAAAACAGGGCAGACATTATAAAATCGTCGACAAACAATGGAAATCGGTACGCCACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc         <  2:1797577/140‑1 (MQ=255)
                                                                                                                                   aTTTGTTACAAAAACAGGGCAGAGATTATAAAATCGTCGAAAAAGAATGGAAATGGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc         <  2:1418409/140‑1 (MQ=255)
                                                                                                                                   aTTTGTTAAAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAATTGGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTTACATTAAACGACGGTGATTACATGAAAAATAAc         <  2:6042850/140‑1 (MQ=255)
                                                                                                                                   aTTGGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCTACAAAGAATGGAAAGCGGTACGCGACCAGCTCGTGAGCATGGGGGTAACCGGCGGGTTTCCATATTTGACATTAAACGACGGTGATTACATGAAAAATAAc         <  2:1684098/140‑1 (MQ=255)
                                                                                                                                      tGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAg      <  2:450287/140‑1 (MQ=255)
                                                                                                                                       gTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAATAATGCAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGc     <  2:1008456/140‑1 (MQ=255)
                                                                                                                                       gTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                    <  2:5050635/61‑1 (MQ=255)
                                                                                                                                       gTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGttt                                                   >  1:3403805/1‑94 (MQ=255)
                                                                                                                                       gTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGc     >  1:1797577/1‑140 (MQ=255)
                                                                                                                                       gTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAAAAACGAGc     >  2:4339104/1‑140 (MQ=255)
                                                                                                                                        tttCAAAAACAGGGCAGATATTATAAAATCGTCGACAAAGAATGTAAATCGGTACGCGACCAGCTCGTGAGCATGCGGTTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct    <  2:245251/140‑1 (MQ=255)
                                                                                                                                        tttCAAAAACAGGGCAGAGATTATAAACTCTTCGACAAACAAGGGAAATCGGTACGCGCCCAGCTCGTGCGCAGGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct    <  2:7611368/140‑1 (MQ=255)
                                                                                                                                        tttCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACATTAAACGACGGTGATTACATGAAAAATAACGAGct    <  2:1228848/140‑1 (MQ=255)
                                                                                                                                        tttAAAAAACTGGGCATATATTATAAAATCGTCTACAAAGATTGCAAATGGGTACGCGACCAGGTGGTGAGCATGGGGTTAAACGGGGGGTTTCAATATTTGACTGTACACGACGGTGATTACATGAAAAATAACGAGct    <  1:6497321/140‑1 (MQ=255)
                                                                                                                                        tttAAAAAACAGGGCATATATTATAAAATCGTCGAAAAACAATGTAAATGGGTACGCCACCAGCTCGTGAGCATGCGGTTAAACGGGGGTTTTCCATATTTGACATTAAACGACGGTGATTACATGAAAAATAACGAGct    <  1:3809130/140‑1 (MQ=255)
                                                                                                                                        tttAAAAAACAGGGCAGAGATTATAAAATGGTCGAAAAAGATTGGAAATCGGTACGCCACCCGCTCGTGAGAATGCGGTTAAACGGCGGTTTTCCATATTTCACAGTAAACGACGGTGATTACATGAAAAATAACGAGct    <  1:3614198/140‑1 (MQ=255)
                                                                                                                                        tttAAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCTTGCGGTTAAACGGGGGGTTTCCATATTTGAAAGTAAACGACGGTGATTACATGAAAAATAACGAGct    <  1:7268859/140‑1 (MQ=255)
                                                                                                                                        tttAAAAAACAGGCCATAGATTATAAAATCTACCAAAAATATGGCAAAGCGGTACGCCCCCAGCTGTTGAGAAGGGGGTTAAAGGGGGGTTTTCAATATTTTACATTAAACGACGGTGATTACATGAAAAATAACGAGct    <  1:2016649/140‑1 (MQ=255)
                                                                                                                                        ttacAAAAACTGGGCATAGTTTATAAAATCGTCGACAAAGATTGGAAATGGGTACGCGACCTGCTCGTGAGCATGGGGTTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct    <  1:6371859/137‑1 (MQ=255)
                                                                                                                                        ttacAAAAACAGGGCCTACATTATAAAATCGTCCACAAACAATGCAAATCGGTCCGCGACCAGCTCTTGAGCATGCGGGTAAACGCCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct    <  2:7346334/137‑1 (MQ=255)
                                                                                                                                        ttacAAAAACAGGGCAGTGATTATAAATTCGTCGACAAACAATGTAAATCGGTACGCGACCCGGTGGTGAGCTTGGGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct    <  1:396890/137‑1 (MQ=255)
                                                                                                                                        ctccAAAACCCGGCCAGATATTATAAAATCTTCGCAAAAGATTGCAATTCGGTACGCGACAAGCTCGTGAGCATGCGGGTAACCGGGGGTTTTCAATATTTCACAGTAAACGACGGTGATTACATGAAAAATAACGAGct    <  1:3228198/137‑1 (MQ=255)
                                                                                                                                          tCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGctc   >  1:1742463/1‑139 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AGAAATGAAGAAAATGGGCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCT  >  NZ_CP034943/986647‑986924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: