Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,038,838 C→A 6.7% Q30K (CAA→AAA)  EO946_RS05365 → AAA family ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,038,8380CA 6.7% 1012.8 / 9.3 410Q30K (CAA→AAA) EO946_RS05365AAA family ATPase
Reads supporting (aligned to +/- strand):  ref base C (180/185);  new base A (2/42);  total (182/228)
Fisher's exact test for biased strand distribution p-value = 8.41e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 440 total aligned reads displayed.

CAGGCACAGATTTTATTGAAAAGACAGCTTAAGGTGCTTGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGGTGTCTATGGCGGTGTTCTTGAAGCTGAAC  >  NZ_CP034943/1038699‑1038976
                                                                                                                                           |                                                                                                                                          
cAGGCACAGATTTTATTGAAAAGACAGCTTAAGGTGCTTGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTcc                                                                                                                                            >  1:1539675/1‑140 (MQ=255)
cAGGCACAGATTTTATTGAAAAGACAGCTTAAGGTGCTTGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTcc                                                                                                                                            >  1:6801069/1‑140 (MQ=255)
cAGGCACAGATTTTATTGAAAAGACAGCTTAAGGTGCTTGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTcc                                                                                                                                            <  2:7407542/140‑1 (MQ=255)
cAGGCACAGATTTTATTGAAAAGACAGCTTAAGGTGCTTGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTcc                                                                                                                                            >  2:5339380/1‑140 (MQ=255)
cAGGCACAGATGTTATTGAAAAGACAGCTTAAGGTGCTTGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTcc                                                                                                                                            <  2:4424273/140‑1 (MQ=255)
   gCACAGATTTTATTGAAAAGACAGCTTAAGGTGCTTGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAAt                                                                                                                                         >  2:2088267/1‑140 (MQ=255)
        gATTTTATTGAAAAGACAGCTTAAGGTGCTTGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATc                                                                                                                                    >  2:112063/1‑140 (MQ=255)
          ttttATTGAAAAGACAGCTTAAGGTGCTTGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTa                                                                                                                                  <  1:4622633/140‑1 (MQ=255)
          ttttATTGAAAAGACAGCTTAAGGTGCTTGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTa                                                                                                                                  <  1:2116369/140‑1 (MQ=255)
          ttttATTGAAAAGACAGCTTAAGGTGCTTGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTa                                                                                                                                  <  1:4053380/140‑1 (MQ=255)
           tttATTGAAAAGACAGCTTAAGGTGCTTGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTAc                                                                                                                                 <  1:1032873/140‑1 (MQ=255)
             tATTGAAAAGACAGCTTAAGGTGCTTGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTTTCCAATTAATCTACgg                                                                                                                               >  2:1459754/1‑140 (MQ=255)
              aTTGAAAAGACAGCTTAAGGTGCTTGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGa                                                                                                                              <  1:5023054/140‑1 (MQ=255)
                    aaGACAGCTTAAGGTGCTTGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAAt                                                                                                                        <  1:7512612/140‑1 (MQ=255)
                         atcTTAAGGTGATTTAAATATTAGGCGTTGAAAGCTTTGCTAATTAAATCTTTAAATATATACCAATACGGTAAATTTTCCAATCAGACCTTTCAATTTTCCGCCTCGCCTTTCAAATTAATCTACGTATTAATTGAgga                                                                                                                   <  2:7260519/138‑2 (MQ=255)
                                     ttGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACg                                                                                                                                <  2:6380025/115‑1 (MQ=255)
                                     ttGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACg                                                                                                                                >  1:6380025/1‑115 (MQ=255)
                                     ttGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACg                                                                                                                                >  1:6218726/1‑115 (MQ=255)
                                     ttGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAacgac                                                                                                       >  2:6151204/1‑140 (MQ=255)
                                     ttGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAacgac                                                                                                       >  2:2980290/1‑140 (MQ=255)
                                     ttGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAacgac                                                                                                       >  2:3019072/1‑140 (MQ=255)
                                     ttGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAacgac                                                                                                       >  2:7649822/1‑140 (MQ=255)
                                     ttGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAacgac                                                                                                       >  2:4978200/1‑140 (MQ=255)
                                     ttGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAacgac                                                                                                       >  2:4202647/1‑140 (MQ=255)
                                     ttGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATAAGACCTTTCACTTTTCCGCATCGCCTTTCCAATTCATCTACGGATTAAATGACTCAGGAAAAacgac                                                                                                       >  2:2569627/1‑140 (MQ=255)
                                     ttGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATAAGACCTTTCACTTTTCAGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAacgac                                                                                                       >  2:5651593/1‑140 (MQ=255)
                                         aaTTTGAGGCGGTTAAAGCTTTGCTAATTAAATATTTACATATCTACAAATACGGGAAATTTTCAAATCACACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGTTTTAAATGAGGCAGCAAAAACGACgatg                                                                                                   <  1:1841441/140‑1 (MQ=255)
                                           tatGAGGCGGTTAAAGCTTGGCGAATTAAATCTTTACATATCTCCAAATACGGCAAATTTTCCAATCAGCCCTTTCAGTTTTCCCCCCCGCCTGTCCATTTAATCTACGGATAAAATGAGGCAGGATAAACGACgatgat                                                                                                 <  1:1330768/140‑1 (MQ=255)
                                           catGAGGCGCTGACAGCTTGCCGAATTAAAACTTTACCTAGCACCCAATCCGGGAATTTTTCCAATCAGACCTTCCACTTTTCCGCCTCGCCTGTCCAATTTCTCTACGGATTAAATGAGGCAGGAAAAACGACgatgat                                                                                                 <  2:7750367/139‑1 (MQ=255)
                                            atGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGatgatg                                                                                                >  2:7324884/1‑140 (MQ=255)
                                              gAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTc                                                                                              >  2:4007082/1‑140 (MQ=255)
                                              gAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTAAGCCTCGCCTTTCCAATTAATATAAGGATTAAATGAGCCAGGAAAAACGACGATGATGTc                                                                                              >  2:3192307/1‑140 (MQ=255)
                                               aGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCa                                                                                             >  2:2320505/1‑140 (MQ=255)
                                               aGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCAAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACTATGATGTCa                                                                                             >  2:2598147/1‑140 (MQ=255)
                                                ggCGGTGACAGCTTTGCGAATTAAATCTTACCATATCTCCCAATCCGGCAAATTTTCCAATCAGACCTTTCACTTTGCCGCCTCGCCTGTCCAATTAATCTCCGGATTAAATGAGGCAGCAAAAACGACGATGATGTCAt                                                                                            <  1:5520323/140‑1 (MQ=255)
                                                ggCGGTCAAAGGTTTGCTAATTAAATCTTTACATATCTACAAATACGGTAAATTTTCCAATCAAACCTTAAACTTTTCCGCCTCGCCTGTCCATTTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCAt                                                                                            <  1:3257400/140‑1 (MQ=255)
                                                   ggTGAAAGCTTTGCGACTTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCGACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATGTa                                                                                         >  2:885506/1‑140 (MQ=255)
                                                     tGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTAtt                                                                                       >  1:7454378/1‑140 (MQ=255)
                                                     tGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCAGTTAtt                                                                                       >  1:944105/1‑140 (MQ=255)
                                                            tttGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCa                                                                                >  2:790019/1‑140 (MQ=255)
                                                              tGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAAt                                                                                                                        <  2:3363347/98‑1 (MQ=255)
                                                              tGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACTATCATGTCATTTATTGAAAGCATg                                                                              >  1:7768962/1‑140 (MQ=255)
                                                              tGCGAATTAAATCTTTAAATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCAGTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATg                                                                              <  2:4087088/140‑1 (MQ=255)
                                                                             tACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCcg                                                               >  2:4763255/1‑140 (MQ=255)
                                                                             tACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCcg                                                               >  2:6949842/1‑140 (MQ=255)
                                                                               aatatCTACCAATACGTTAAATTTTCAAAACAGACCTTTCACTTTTCCGCCTCGCCTGTCAAATTAATATACTTATTAATTGAGGAAGTAAAAACGACGATGATGTAATTTATTGAAAGCATGCTTTTTGGTTTTCcgaa                                                             <  1:344436/139‑1 (MQ=255)
                                                                                  atCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCcgaaaac                                                          >  2:5153621/1‑140 (MQ=255)
                                                                                   tCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCcgaaaacg                                                         >  1:4236367/1‑140 (MQ=255)
                                                                                       ccAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTTAAAGCATGCTGTTTGGTTTTCcgaaaacgacaa                                                     >  2:6267187/1‑140 (MQ=255)
                                                                                        cAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCAGTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGaaaaa                                                    >  1:6582556/1‑140 (MQ=255)
                                                                                          aTACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAat                                                  >  2:5722413/1‑140 (MQ=255)
                                                                                               ggAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGAGGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTACGAAAACGAAAAAATATGaa                                             >  2:4513267/1‑140 (MQ=255)
                                                                                                   aTTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGAGGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGa                                         >  2:5702259/1‑140 (MQ=255)
                                                                                                    ttttCCAATCAGACCTTTCAATTTTCCGCCTCGCCTGTCAAATTAATATACGGATTAATTGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGaa                                        <  2:1204255/140‑1 (MQ=255)
                                                                                                          aaTCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGTATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAgg                                  <  1:1183118/140‑1 (MQ=255)
                                                                                                           aacAGACCTTTCAATTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGc                                 <  1:5945762/138‑1 (MQ=255)
                                                                                                           aTCAGACCTTTCAATTTTCCGCCTCGCCTGTCAAATTAATCTACGGATTAAATTAGGCATGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGc                                 <  1:7020310/140‑1 (MQ=255)
                                                                                                            tctgACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATGTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCg                                <  2:4343483/137‑1 (MQ=255)
                                                                                                            tCAGACGTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAGAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCg                                <  2:1901876/140‑1 (MQ=255)
                                                                                                            tCAGACGTTTCACTTTTCCGCCTCGCCTGTCAAATTAATCTACGTATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCg                                <  2:2429052/140‑1 (MQ=255)
                                                                                                            tCAGACCTTTCACTTTTCCGCCTCGCCTTTCCAATTAATATCCGGATTAAATGTGGCAGTAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCg                                <  1:6642406/140‑1 (MQ=255)
                                                                                                            tCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCg                                <  1:2480415/140‑1 (MQ=255)
                                                                                                            tCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCg                                <  2:5866823/140‑1 (MQ=255)
                                                                                                            tCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCg                                <  2:3716587/140‑1 (MQ=255)
                                                                                                            tCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGCAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCg                                <  1:5339380/140‑1 (MQ=255)
                                                                                                            tCAGACCTTTCACTTTTCCGCCTCGCCTGTCAAATTAATATACGTATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCg                                <  2:6902211/140‑1 (MQ=255)
                                                                                                            tCAGACCTTTCACTTTTCCGCCTCGCCTGCCCAATTAATCTACGGATTAAATGAGGCAGTAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCg                                <  1:2601482/140‑1 (MQ=255)
                                                                                                            tCAGACCTTTCACTTTGCCGCCTCGCCTGTCAAATTAATCTACGTATTAAATGAGGCAGTAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCg                                <  1:2002054/140‑1 (MQ=255)
                                                                                                            tCAGACCTTTCACTTTACCGCCTCGCCTGTCCAATTAATCTCCGCATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCg                                <  2:4811019/140‑1 (MQ=255)
                                                                                                            tCAGACCTTTCACTTGTCCGCCTCGCCTGTCCAATTACTCTACGTATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCg                                <  2:5985059/140‑1 (MQ=255)
                                                                                                            tCAGACCTTACACTTTTCCGCCTCGCCGGCCCAATTAATCTACGTATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCg                                <  1:1423950/140‑1 (MQ=255)
                                                                                                            acataCCTTTCACTTTTCCGCCTCGCATGTCCAATTAATCTACGGTTTAAATGAGGCAGGAAAAACGACGATGTTGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACCGGCg                                <  2:5810995/136‑1 (MQ=255)
                                                                                                            acAGCCCTTTCACTGGCCCGCCCCGCCGGTCCAATTAATCGACGGATTAAATGAGCCAGGAAACACGACGATGATGACATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCg                                <  1:1215371/139‑1 (MQ=255)
                                                                                                            acAGACATTTCACTTTTCCGCCTCGCCTGTCAAATTAATCTACGGATTAAATGAGGCAGTAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCg                                <  1:2271473/139‑1 (MQ=255)
                                                                                                              cgcccTTTCACTTTTCGGCCTCGCGTGTCAATTTATTATACGGATTAAATTAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGgt                              <  1:3694519/137‑1 (MQ=255)
                                                                                                              acaCCTTACACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGgt                              <  1:3069979/138‑1 (MQ=255)
                                                                                                              aGACCTTTCACTTTAACGCCTCGCATTTCCACTTAATCTCCGTATAAAATGAGGCTTGAAAAAGGACGATGAGGTCATTTATTTAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGgt                              <  1:3871222/140‑1 (MQ=255)
                                                                                                              aGACCTTTAAATTTTCCGCCTCGCCTGTCCAATTACTCTACGGATTAAATTAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGgt                              <  1:4118825/140‑1 (MQ=255)
                                                                                                              aGACCTGTCACTTTTCCGCCGCGCGTGTCCAATTATTCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGgt                              <  2:414624/140‑1 (MQ=255)
                                                                                                                    ttCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGGTGTCTAt                        >  1:7672321/1‑140 (MQ=255)
                                                                                                                     tCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGGTGTCTATg                       >  1:2069875/1‑140 (MQ=255)
                                                                                                                     tCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGGTGTCTATg                       >  1:5771184/1‑140 (MQ=255)
                                                                                                                          tttCCGCCTCGCGTTCCCACTTAATCTACGTATTAAATGAGGCAGCAAAAACGACGATGATGTCATTTATTGAAAGGATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGGTGTCTATGGCGgt                  <  2:661399/140‑1 (MQ=255)
                                                                                                                          tgtCCGCCTCGCCTGTCCAATTAATCTACGTATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGGTGTCTATGGCGgt                  <  1:4747369/138‑1 (MQ=255)
                                                                                                                               gcctcgcctGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGGTGTCTATGGCGGTGTTCt             >  2:7116025/1‑140 (MQ=255)
                                                                                                                                   ctcctGTCCAAGTCATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTCATTGAAAGCATGCTGTTCGGTTTTCCGAAAACGAAAAAGTATGAACCGAAAACAGGCGGTGTCTACGGCGGTGTTCTTGaa         <  1:2371830/138‑1 (MQ=255)
                                                                                                                                   cgcctGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGGTGTCTATGGCGGTGTTCTTGaa         <  2:5455387/140‑1 (MQ=255)
                                                                                                                                   cgcctGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGGTGTCTATGGCGGTGTTCTTGaa         <  2:1659389/140‑1 (MQ=255)
                                                                                                                                    gcctGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGGTGTCTATGGCGGTGTTCTTGAAg        <  2:4001214/140‑1 (MQ=255)
                                                                                                                                       tGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGGTGTCTATGGCGGTGTTCTTGAAGCTg     <  1:444511/140‑1 (MQ=255)
                                                                                                                                       tGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGGTGTCTATGGCGGTGTTCTTGAAGCTg     <  2:3794748/140‑1 (MQ=255)
                                                                                                                                       tGTCAAATTAATCTACGGATTAAATGAGGCAGTAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGGTGTCTATGGCGGTGTTCTTGAAGCTg     <  1:7126877/140‑1 (MQ=255)
                                                                                                                                        gTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGGTGTCTATGGCGGTGTTCTTGAAGCTGa    <  1:6061245/140‑1 (MQ=255)
                                                                                                                                        gTCCAATGAATCTACGGATTAAATGAGCCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGGTGTCTATGGCGGTGTTCTTGAAGCTGa    <  2:6472787/140‑1 (MQ=255)
                                                                                                                                         tCCAATTAATCTACGGATTAAATGAGGCAGTAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACATGCGGTTTATATTGCGGTGTTCTTGAAGCTGaa   <  2:3489463/140‑1 (MQ=255)
                                                                                                                                          ccATTTATTCTAAGTATTAAATTATGAAGTAAAAAAGTCTATGATGTCATTTATTGAAAGCATGCTGTTTTGTTTTCCGAAAACGAAATAATATGAACAGACAACAGGCGGTGTCTATGGCGGTGTTCTTGAAGATGTAc  <  2:1362764/140‑1 (MQ=255)
                                                                                                                                          ccAATTAATCTCCGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGGTGTCTATGGCGGTGTTCTTGAAGCTGAAc  <  1:2625730/140‑1 (MQ=255)
                                                                                                                                          ccAATTAATCTACGTTTTAATTTAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGGACCGAAAACAGGCGGTGTCTATGGCGGTGTTCTTGAAGCTGAAc  <  1:4395280/140‑1 (MQ=255)
                                                                                                                                          ccAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGGTGTCTATGGCGGTGTTCTTGAAGCTGAAc  <  2:1948724/140‑1 (MQ=255)
                                                                                                                                          acAATTAATCTACGTATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGGTGTCTATGGCGGTGTTCTTGAAGCTGAAc  <  2:2207291/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CAGGCACAGATTTTATTGAAAAGACAGCTTAAGGTGCTTGAAATATGAGGCGGTGAAAGCTTTGCGAATTAAATCTTTACATATCTACCAATACGGGAAATTTTCCAATCAGACCTTTCACTTTTCCGCCTCGCCTGTCCAATTAATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTTATTGAAAGCATGCTGTTTGGTTTTCCGAAAACGAAAAAATATGAACCGAAAACAGGCGGTGTCTATGGCGGTGTTCTTGAAGCTGAAC  >  NZ_CP034943/1038699‑1038976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: