Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,097,455 C→A 11.4% I66I (ATC→ATA EO946_RS05675 → MarR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,097,4550CA11.4% 596.6 / 17.6 261I66I (ATC→ATAEO946_RS05675MarR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (121/100);  new base A (4/36);  total (125/136)
Fisher's exact test for biased strand distribution p-value = 6.61e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

AGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGC  >  NZ_CP034943/1097316‑1097594
                                                                                                                                           |                                                                                                                                           
agcagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatc                                                                                                                                             >  1:3056702/1‑140 (MQ=255)
 gcagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatca                                                                                                                                            <  2:5725947/140‑1 (MQ=255)
 gcagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatca                                                                                                                                            <  1:7308712/140‑1 (MQ=255)
 gcagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatca                                                                                                                                            >  2:3179627/1‑140 (MQ=255)
 gcagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatca                                                                                                                                            >  2:6478792/1‑140 (MQ=255)
  cagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGTACAATGAACcaatcaa                                                                                                                                           >  1:7454123/1‑140 (MQ=255)
  cagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatcaa                                                                                                                                           >  1:1021257/1‑140 (MQ=255)
  cagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatcaa                                                                                                                                           >  1:6138827/1‑140 (MQ=255)
   agGAGAAATGGGAGGGGCTGTTTATAGCCTTTTTGGCTGACGAATTGAAGAATCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGAAAGTAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatcaat                                                                                                                                          <  1:3670675/140‑1 (MQ=255)
     gagaAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGt                                                                                                                                        >  2:3309793/1‑140 (MQ=255)
     gagaAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGt                                                                                                                                        >  1:1373506/1‑140 (MQ=255)
     gagaAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGt                                                                                                                                        >  1:3464194/1‑140 (MQ=255)
        aaaTGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCAc                                                                                                                                     <  1:6596840/140‑1 (MQ=255)
        aaaTGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCAc                                                                                                                                     <  1:4039406/140‑1 (MQ=255)
        aaaTGGGAGGGGCTCATTAAAGCCTGTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCAc                                                                                                                                     <  1:4307870/140‑1 (MQ=255)
               aGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACCATGAACCAATCAATGTCACGTCTAtt                                                                                                                              >  2:2659685/1‑140 (MQ=255)
               aGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGt                                                                                                                                        <  2:7544041/130‑1 (MQ=255)
               aGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTAtt                                                                                                                              >  1:1820053/1‑140 (MQ=255)
               aGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTAtt                                                                                                                              >  2:309640/1‑140 (MQ=255)
                 gggCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGc                                                                                                                            >  1:678755/1‑140 (MQ=255)
                  ggCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCa                                                                                                                           >  1:4984774/1‑140 (MQ=255)
                       aTTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGaaaa                                                                                                                      >  1:5857597/1‑140 (MQ=255)
                       aTTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTGGCTTGTGTTGGGGCCCATGACCCAATCAATGTCACGTCTATTGCAGaaaa                                                                                                                      >  2:7616854/1‑140 (MQ=255)
                            aGCCTTTTTGGCTTACGAATTTAATAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCTATTACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATAAATGTCACGTCTATTGCAGAAAAAATGa                                                                                                                 >  2:7091904/1‑140 (MQ=255)
                             gCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGaa                                                                                                                <  2:677944/140‑1 (MQ=255)
                             gCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGaa                                                                                                                <  1:5159720/140‑1 (MQ=255)
                             gCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTAGTGCAGAAAAAATGaa                                                                                                                <  2:2908460/140‑1 (MQ=255)
                              ccTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCACTCAATGTCACGTCTATTGCAGAAAAAATGAAt                                                                                                               >  2:1561837/1‑140 (MQ=255)
                               cTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATa                                                                                                              <  2:5377885/140‑1 (MQ=255)
                                 ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCAGGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                            >  2:5264981/1‑140 (MQ=255)
                                 ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                            >  1:6992092/1‑140 (MQ=255)
                                 ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                            >  1:1870841/1‑140 (MQ=255)
                                 ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                            >  1:7566682/1‑140 (MQ=255)
                                 ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTGGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                            >  1:5348610/1‑140 (MQ=255)
                                 ttttGGCTGACGAATTGAAGAAGCTCGATGTAGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                            >  1:4680325/1‑140 (MQ=255)
                                   ttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGAGCATGAACCAATCAATGTCACGTCTCTTGCAGACAATATGAATACGAc                                                                                                          >  1:6126504/1‑140 (MQ=255)
                                      gcttaggAATTGAAGACGATCGTTGTCGAGCACGGGAGTAAATCTAATTTATCTATGACATAAATTCTTGTTTTTTTTTGTGTTGGGGAAAATGAACAAATCAATGTCAGGTTTTTTGCATAAAAAATGAATACGAaaaa                                                                                                       <  2:3464194/134‑1 (MQ=255)
                                        tGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAg                                                                                                     >  2:2086518/1‑140 (MQ=255)
                                        tGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAg                                                                                                     >  2:194693/1‑140 (MQ=255)
                                          aCGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCg                                                                                                   >  2:6179498/1‑140 (MQ=255)
                                          aCGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCg                                                                                                   >  1:1676765/1‑140 (MQ=255)
                                          aCGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATAAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCg                                                                                                   >  2:454862/1‑140 (MQ=255)
                                           cGAATTGAAGAAGCTCGATGTCGAGAAGGGGATAAAATCTAAATTATCGATGAATAAAATTAATGTTCTTTCTTTTTTTGGGTACAATTAACAAATAAATGTCACGTCTATTCCAGAAAAAATTAAAACGACAAACGCGa                                                                                                  <  2:1373506/140‑1 (MQ=255)
                                                        cTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAACAGCGACGGTATCGCGCAt                                                                                     >  2:4330137/1‑140 (MQ=255)
                                                            aTGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGc                                                                                 >  1:1628982/1‑140 (MQ=255)
                                                              gTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGcac                                                                               >  1:5402071/1‑140 (MQ=255)
                                                                 gAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACaaa                                                                            >  1:4414808/1‑140 (MQ=255)
                                                                 gAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACaaa                                                                            >  1:2982394/1‑140 (MQ=255)
                                                                  aGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACaaaa                                                                           >  1:2330973/1‑140 (MQ=255)
                                                                  aGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACaaaa                                                                           >  1:1076163/1‑140 (MQ=255)
                                                                  aGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGGTCTTTCTGGTGTTGGGGACAATGAACCAATCAATGTCACGTCTCTTCCAGAAAAAATCAATACGACAAAAGCGACTGTATCGAGCATCAGCACaaaa                                                                           >  2:1655281/1‑140 (MQ=255)
                                                                            tAAATATCAATTATCGAGTACAGAAATTCATGTTCTTTCTTGTGTGGGGGACAATGAACCAATCAATGTCACGTATTTTGCATAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGgcg                                                                 <  2:5995622/140‑1 (MQ=255)
                                                                               aTCTAATTTATCGATTAAATAATTTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCACTGTCACTTCTATTGAAGAAAAAATTAATACGAAAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCgg                                                              <  2:4124222/140‑1 (MQ=255)
                                                                                 ctcATTTCTCGAGCACACAAATACATGTTCTTTGTTGTGTTGGGCACAATGAACCAATCACTGTCACGTATATGCCACAAAAAATCAATACCCCAACCGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGt                                                            <  1:1158716/140‑1 (MQ=255)
                                                                                 ctcAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGt                                                            >  1:3118860/1‑140 (MQ=255)
                                                                                 ctcAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGt                                                            >  2:5700126/1‑140 (MQ=255)
                                                                                  tcAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGtt                                                           >  1:7124366/1‑140 (MQ=255)
                                                                                   cAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGttt                                                          >  1:6502238/1‑140 (MQ=255)
                                                                                    aaTTATCGATGACAGAAATTCATGTTCTTTGTTGTGTGGGGTAAAATCAACCAATCATTGTCACGTCTATTGCATAAAAAATCAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTc                                                         <  2:4133910/140‑1 (MQ=255)
                                                                                      ttATCTATGACAGAATTTCTTGTTTTTTCTTGTGTTGGGGACAATGAACCAATCATTGTCACTTATATTGCACAAAAAATCAATACGACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCt                                                       <  1:6077762/140‑1 (MQ=255)
                                                                                      ttATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTTTGGCAGAAAAAATGAATACGACAACAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCt                                                       <  2:402668/140‑1 (MQ=255)
                                                                                      ttATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCt                                                       <  2:1870841/140‑1 (MQ=255)
                                                                                      ttATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATAAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCt                                                       <  2:5772990/140‑1 (MQ=255)
                                                                                      ttATCGATGAAATAATTTCATTTTCTTTCTTGTGTTGGGGAAAATGAACCAATAAATGTAACTTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCt                                                       <  2:5248387/140‑1 (MQ=255)
                                                                                      ttATCGATCACACAATTTCTTTTTCTTTTTTGTGTGGGCCAAAATCAACAAATAAATGTCACGTCTATTGCATAAAAAATCAATCCCACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCt                                                       <  1:15642/140‑1 (MQ=255)
                                                                                         tctaTGACATAAATTCATGTTCTTTATTGTGTTGGGCAAAATGAACAAATAATTTTCACGTCTATTGAAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                    <  2:2484959/137‑1 (MQ=255)
                                                                                         tcgagtaCATAAATTATTTTTCTTTCTTGTGTTGGGGACAATCAACCAATCAATTTCACGTATATTGCACAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                    <  1:5675113/134‑1 (MQ=255)
                                                                                         tCGATGACATAAATTCTTTTTCTTTGTTGTGTTGGGGAAAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATTAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                    <  2:5518596/140‑1 (MQ=255)
                                                                                         tCGATGACAGAATTTCTTTTTCTTTCTTGTGTTGGGGAAATTTAACAAATAATTGTCAGGTATTTGGCCAAAAAAATAAAACCGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                    <  1:7228308/140‑1 (MQ=255)
                                                                                         tCGATGACAGAAATTCTTGTTCTTTCTTTTGTTGGGTAAAATGAACAAATAATTGTCACGTCTATTGCACAAAAATTGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                    <  2:1117881/140‑1 (MQ=255)
                                                                                         tCGATGACAGAAATCCATGTTATTTCTTTTGTTGGGGACAATGACGCAATCACTGTCACTTATATTGCATAAAAAATGAAAACGACAACCGCGACTGTATCGCGCCTCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                    <  2:1735617/140‑1 (MQ=255)
                                                                                         tCGATCACAAAAATTCTTTTTCTTTGTTGTGTTGGGGACAATCAACCAATAAATGTCACGTCTTTTGCACAAAAAATCAATCCGACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                    <  1:6273298/140‑1 (MQ=255)
                                                                                                      ttCTTGTTCTTTCTTGTGTTGGGGACAATGAACCAATAAATGTCACGTATATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTc                                       <  2:4984774/140‑1 (MQ=255)
                                                                                                           tttctttctTGTTTTGGGGAAAAGGAACCAATCAAGGTCACGTATCTTGCATAAAAAATGAAGCCGACACACGCGACTGTCTCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACATAACACAGCTCAGCGa                                  <  2:3919920/139‑1 (MQ=255)
                                                                                                           tttctttctTGTTTGGGGGACAATGACCCAATCATTGTCACGTCTATTGCACAAAAAATGAAGCCGAAAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa                                  <  1:1490401/139‑1 (MQ=255)
                                                                                                           gTTCTTTTTTGTGTGGGGGACACTTAACCAATCAATGTCAGGTCTATTGCACAAAAAATGAATACTACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa                                  <  2:5822600/140‑1 (MQ=255)
                                                                                                           gTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa                                  >  1:2531315/1‑140 (MQ=255)
                                                                                                            ttctttctTGTGTTTGGGACAATCAACCAAGCAATGTCACGTCTATTGCATAAAAAATGAACACCACAAAAGCGACTCTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGAt                                 <  2:7659986/140‑1 (MQ=255)
                                                                                                            ttattttttGTGTTGGGGACAATCAACCAATAAATGTCACGTATATTGCATAAAAAATTAATACCAAAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGAt                                 <  1:1585966/137‑1 (MQ=255)
                                                                                                                 tcttGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACaa                            >  1:994467/1‑140 (MQ=255)
                                                                                                                   ttggtttGGGGACAATTAACAAACCATTTTCACTTCTTTTCCATAAAAATTGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACaaaa                          <  1:2676377/135‑1 (MQ=255)
                                                                                                                     gtgtGGGGCAAAATGAACCAATCATTGTCACGACTATTGCAGAAAAAATGAATACCAAAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAag                        <  1:5608573/140‑1 (MQ=255)
                                                                                                                      ttttGGGCAAAATTAACAAATAATTGTCACGTATTTTGAACAAAAAATCAATACCACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAagg                       <  1:2488337/138‑1 (MQ=255)
                                                                                                                      tgtgGGGCAAAATCAACAAATCAATGTCACGTATATTGCATAAAAATTTAATCCCAAAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAagg                       <  1:3309793/140‑1 (MQ=255)
                                                                                                                      tgtTGGGGAAAATGAACCAATCATTGTCACGTATATTCCATAAAAAATCAATACGACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAagg                       <  1:7091904/140‑1 (MQ=255)
                                                                                                                      tgtTGGGCAAAATCAACAATTCATGGTAACGTATTTTGAATAAAAAATTAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAagg                       <  1:2770733/140‑1 (MQ=255)
                                                                                                                        ttGGGGACAATGAACCAATCAATGTCAGGTTTATGGCCTAAAAAATTAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAaggag                     <  1:7531048/140‑1 (MQ=255)
                                                                                                                          ggtcaaaaTCAACAAATAAATGTAACGTCTTTTCCACAAAAAATTAATCCCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGt                   <  1:7059990/134‑1 (MQ=255)
                                                                                                                          ggggAAAATCAACCAATAAATGTCACTTCTTTTTCACAAAAAATGAATACGAAAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGt                   <  2:5104881/140‑1 (MQ=255)
                                                                                                                           gtgACAATGAACAAAACAATGTCACGTATATTGCATAAAAAATTAATACGACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTc                  <  2:7350509/138‑1 (MQ=255)
                                                                                                                           gggACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTc                  >  1:4567417/1‑140 (MQ=255)
                                                                                                                           gggACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTc                  >  1:2271911/1‑140 (MQ=255)
                                                                                                                           gggACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTc                  >  1:2234970/1‑140 (MQ=255)
                                                                                                                                aaTGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAaca                                             <  2:5635934/108‑1 (MQ=255)
                                                                                                                                aaTGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAaca                                             >  1:5635934/1‑108 (MQ=255)
                                                                                                                                aaTGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGGATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATCACAAAAAGGAGGTCTACtg             >  1:3933771/1‑139 (MQ=255)
                                                                                                                                aaTGAACAAATCAATGTCACGTCTTTTGCATAAAAAATGAATACGACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACtt             <  1:7752949/140‑1 (MQ=255)
                                                                                                                                  tGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTcc           >  1:942980/1‑140 (MQ=255)
                                                                                                                                          tcaatGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATCACAAAAAGGAGGTCTACTTCCGATTGACg   >  1:5700180/1‑140 (MQ=255)
                                                                                                                                          tcaatGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACg   >  1:6825313/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTGACGC  >  NZ_CP034943/1097316‑1097594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: