Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,293,306 G→T 7.3% V127L (GTA→TTA)  EO946_RS06740 → sigma‑70 family RNA polymerase sigma factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,293,3060GT 7.3% 583.3 / 6.6 215V127L (GTA→TTA) EO946_RS06740sigma‑70 family RNA polymerase sigma factor
Reads supporting (aligned to +/- strand):  ref base G (124/66);  new base T (2/23);  total (126/89)
Fisher's exact test for biased strand distribution p-value = 3.12e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

CCGAGAATCATCGAATCATTTTCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGC  >  NZ_CP034943/1293168‑1293441
                                                                                                                                          |                                                                                                                                       
ccGAGAATCATCGAATCATTTTCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGt                                                                                                                                        >  1:573227/1‑140 (MQ=255)
ccGAGAATCATCGAATCATTTTCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGt                                                                                                                                        >  1:6016635/1‑140 (MQ=255)
        catcGAATCATTTTCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCt                                                                                                                                >  1:4912818/1‑140 (MQ=255)
        catcGAATCATTTTCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCt                                                                                                                                >  1:5580427/1‑140 (MQ=255)
        catcGAATCATTTACCAGCACGATGATGGTGGAGCCGGACGGACAGGTATCAGAAGAAGACACAGAGAGAATCCAAGAAGCGTTCTCCCTGTTAACCGGCAGACAAAAAGAAATCTTTTTGCTGCATAAGTTCGACTGCt                                                                                                                                >  1:7362144/1‑140 (MQ=255)
         atcGAATCATTTTCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGACTCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCtt                                                                                                                               <  2:7298203/140‑1 (MQ=255)
         atcGAATCATTTTCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCtt                                                                                                                               <  2:7433921/140‑1 (MQ=255)
         atcGAATCATTTTCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCtt                                                                                                                               <  1:38772/140‑1 (MQ=255)
         atcGAATCATTTTCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCtt                                                                                                                               <  1:7287183/140‑1 (MQ=255)
                   tttCCAGCACGATGATGGTTGAGCCGGACTGACAGGTATCAGAAGAAGACAGAGAGAGAAGCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGaa                                                                                                                     <  2:2217276/140‑1 (MQ=255)
                     tCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGGGTTATCCCTGTTAACGGGCAGAGAAACAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACg                                                                                                                   <  1:1400506/140‑1 (MQ=255)
                     tCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACg                                                                                                                   <  1:7122020/140‑1 (MQ=255)
                     tCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACg                                                                                                                   <  1:3437692/140‑1 (MQ=255)
                     tCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACg                                                                                                                   <  1:6162196/140‑1 (MQ=255)
                     tCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACg                                                                                                                   <  1:2105400/140‑1 (MQ=255)
                     tCCAGAACGATGATTGTTGATCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGACTCAAAGAAGCGTTATCCCTGTTTACGTGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACg                                                                                                                   <  2:6379105/140‑1 (MQ=255)
                        aGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAAt                                                                                                                >  1:2972907/1‑140 (MQ=255)
                        aGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAAt                                                                                                                >  2:3560061/1‑140 (MQ=255)
                        aGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAAt                                                                                                                >  2:6977229/1‑140 (MQ=255)
                          cACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCg                                                                                                              <  1:1069202/140‑1 (MQ=255)
                          cACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCg                                                                                                              <  1:4338139/140‑1 (MQ=255)
                          cACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCg                                                                                                              <  1:4175207/140‑1 (MQ=255)
                          cACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCg                                                                                                              <  1:2775398/140‑1 (MQ=255)
                            cGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGACGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCt                                                                                                            <  1:2733891/140‑1 (MQ=255)
                            cGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGACTCGCt                                                                                                            <  1:6306833/140‑1 (MQ=255)
                                   ggTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGAtcttc                                                                                                     >  1:480679/1‑140 (MQ=255)
                                    gTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGAtcttct                                                                                                    <  1:1205282/140‑1 (MQ=255)
                                    gTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGAtcttct                                                                                                    <  2:4912818/140‑1 (MQ=255)
                                    gTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGAtcttct                                                                                                    <  1:1632760/140‑1 (MQ=255)
                                       gAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCgg                                                                                                 >  1:6857427/1‑140 (MQ=255)
                                         gCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGgtg                                                                                               >  2:7522503/1‑140 (MQ=255)
                                         gCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGgtg                                                                                               >  2:2752223/1‑140 (MQ=255)
                                             gacggacAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGAtcttct                                                                                                    >  1:750678/1‑131 (MQ=255)
                                              acggacAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTaaaa                                                                                          >  1:5351697/1‑140 (MQ=255)
                                                  acAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAAt                                                                                      >  2:5761093/1‑140 (MQ=255)
                                                  acAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAAt                                                                                      >  2:7592988/1‑140 (MQ=255)
                                                       tATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACt                                                                                 >  2:174666/1‑140 (MQ=255)
                                                       tATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACt                                                                                 >  2:5677543/1‑140 (MQ=255)
                                                       tATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACt                                                                                 >  2:6626207/1‑140 (MQ=255)
                                                           agaagaagaCAGAGAGCGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCTGTGTAAAAAAATCGACTGTGc                                                                             >  1:1491287/1‑140 (MQ=255)
                                                            taagaagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTATAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCa                                                                            >  1:5752662/2‑140 (MQ=255)
                                                                aagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGACATGTTTTTGCTGCCTAAGGTCGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGGAAAAAGATCGACTGTGCACACg                                                                        >  1:4041782/1‑140 (MQ=255)
                                                                aagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACg                                                                                                                   >  2:688124/1‑97 (MQ=255)
                                                                aagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACg                                                                        >  2:7754238/1‑140 (MQ=255)
                                                                aagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACg                                                                        >  2:5433929/1‑140 (MQ=255)
                                                                aagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACg                                                                        >  2:5123003/1‑140 (MQ=255)
                                                                aagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACg                                                                        >  1:1956589/1‑140 (MQ=255)
                                                                  gaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCCAACGAc                                                                      >  1:5035059/1‑140 (MQ=255)
                                                                  gaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGAc                                                                      >  1:5860330/1‑140 (MQ=255)
                                                                  gaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGAc                                                                      >  2:5927732/1‑140 (MQ=255)
                                                                  gaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGAc                                                                      >  1:5379848/1‑140 (MQ=255)
                                                                  gaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGAc                                                                      >  2:3283477/1‑140 (MQ=255)
                                                                       agagagaATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCa                                                                 >  1:4529101/1‑140 (MQ=255)
                                                                       agagagaATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTAGGTGTAAAAAAATCGACTGTGCACCCGACCATCa                                                                 >  1:6964110/1‑140 (MQ=255)
                                                                             aaTCCAAGAAGGGTTATCCATGTTAACGGGAATAGAAAAAGAAATTTTTTTGCTGCATAAGGTAGAATGCTTTTATTATCAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACggg                                                           <  2:7147131/140‑1 (MQ=255)
                                                                             aaTCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACggg                                                           >  2:313918/1‑140 (MQ=255)
                                                                              accaaagaagCTTTATCCTGGTTTCCGCCCATAGAATAACAATTTTTTTTGCTGAATCAGTATTAATTGTTTTCTTATGACCTAATGGATGATTTTCTCTTTTAAAAAAAATCGACTGTGCAAACGACCATCAAACGGGc                                                          <  2:2202686/136‑1 (MQ=255)
                                                                              accaaagaagCGTTAGCCCTGTAACCGGGCAGACAAAAATAATTGTTTTTGCTGCATATGGTCGAATGGTTTTATTATGACCGAATCGCTGATGTTCTGGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGc                                                          <  1:6731111/136‑1 (MQ=255)
                                                                               tCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCg                                                         >  2:5635524/1‑140 (MQ=255)
                                                                                 cAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGAGTGTGCAAACTACCATCAAACGGGCGAt                                                       >  2:2264306/1‑140 (MQ=255)
                                                                                 cAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGAt                                                       >  2:1968476/1‑140 (MQ=255)
                                                                                 cAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGGAAAAAAATCGACTGTGCAAACGACCCTCAAACGGGCgcg                                                       >  1:4091993/1‑138 (MQ=255)
                                                                                   agaagCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACTACCAACAAACGGGCGATCg                                                     >  1:6604669/1‑140 (MQ=255)
                                                                                       ggtttATCCCTTTTAACGGGCAGAGAAAATGAAATGTTTTTGCTGAATAAGTTAGAATGCTTTTATTATGAACTAAACCCTTAACTTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTaaa                                                 <  2:1915258/137‑1 (MQ=255)
                                                                                       gctttttCCCTTTTACCGTGCATATAAAATGAAATTTTTTTTCTGCTTTAGTTATTTTGTTTTTTTTTTTAACCATTGGGTTATGTTGGGGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTaaa                                                 <  2:7029021/134‑1 (MQ=255)
                                                                                       gctttATCCCGTTTACGGGGAATAAAAAAAGAAATGTTTTTGCTGCATAAGTTAGAATGCTTTTCTTATCAACCAATCGGTTATCTTCGGGGTGAAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTaaa                                                 <  1:550151/137‑1 (MQ=255)
                                                                                       gatttATCCCTTTTAACGGCCATAAAAAAATAATTTTTTTTGCTTAATACGGTAGAATGTTTTTCTTATAAACGAATCGCTTATCTTCTCGTTGAAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTaaa                                                 <  1:4840664/137‑1 (MQ=255)
                                                                                       gCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTaaa                                                 >  2:2881639/1‑140 (MQ=255)
                                                                                       cctttATCCCTTTTACCGGGATGTTAAAAAGATATGTTTTTGCTTCATATTGTAAATTTATTTTATTATCAACGAATGGTTTATTTTCTGGTTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTaaa                                                 <  2:3774200/137‑1 (MQ=255)
                                                                                       cctttATCCCTTTAAACGGGCAGACAAAAACAATTGTTTTGGCTGAATACGGTCCACTGCTTTTCTTATCAACCAATGGCTGATGTTCTGGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTaaa                                                 <  1:4938910/137‑1 (MQ=255)
                                                                                            aTCCCTTTTAAGGGGCAGAGAAAAATAAATTTTTTTGGTGCATAAGGTACAATGTTTTTCTTATGAACTAATCGCTTATCTTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGc                                            <  2:6445567/140‑1 (MQ=255)
                                                                                            aTCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGc                                            >  1:809618/1‑140 (MQ=255)
                                                                                             tccgttttAACGGGAATACAAAAATAAATTTTTTTGCTGAATACGGTAGAATGATTTTATTATGAACGAATGGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCa                                           <  1:3709506/134‑1 (MQ=255)
                                                                                             tcagttttAAGGGGAATAAAAAAATAATTTTTTTTGCTGAATTAGTTAGATTGCTTTTATTTTTAACTAATCGCTTTTCTTCTGGGTTAAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCa                                           <  1:2213382/134‑1 (MQ=255)
                                                                                             tCCCTTTAAACGGGCAGACAAAAACAAATTTTTTTGCGGAATATGTTAGAATGCTTTTTTTTTGAACTAATCGCTTATATTCTCGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCa                                           <  1:3626685/140‑1 (MQ=255)
                                                                                                  gTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGAc                                      >  1:2392534/1‑140 (MQ=255)
                                                                                                  gTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGAc                                      >  1:2277581/1‑140 (MQ=255)
                                                                                                       cGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGAcagcag                                 >  1:1126207/1‑140 (MQ=255)
                                                                                                        gggaatagaAAAACAAATTTTTTTGGTGCATAAGGTAGAATGATTTTCTTATCAACTAATCGCTGATCTTCTGGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCagg                                <  2:6188776/134‑1 (MQ=255)
                                                                                                        gggCAGAGAAAAAGAAATTTTTTTGCTGAATAAGTTTGATTGATTTTCTTATTAACGAATCGCTTATCTTCTGGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCagg                                <  2:5325817/140‑1 (MQ=255)
                                                                                                        gggAAGATAAAAATATTTGTTTTTGCTGCATTTTTTTTTTTGCTGTTCTTATGAACTAATCGCTTATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCagg                                <  2:4649697/140‑1 (MQ=255)
                                                                                                         ggCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCagga                               >  1:2997292/1‑140 (MQ=255)
                                                                                                           cAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCTACTGTGCAAACGACAATCAAACGGGAGATCGTAAAGATGCAAAGACAGCaggagg                             >  2:4116237/1‑140 (MQ=255)
                                                                                                           cAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCaggagg                             >  2:3340333/1‑140 (MQ=255)
                                                                                                           cAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGAACATCAAACGGTCCATCGTAAAGATGCAAAGACAGCaggagg                             >  2:4576181/1‑140 (MQ=255)
                                                                                                             gagaAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGaa                           >  2:507994/1‑140 (MQ=255)
                                                                                                             gagaAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGaa                           >  2:6477071/1‑140 (MQ=255)
                                                                                                             gagaAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGaa                           >  1:7171143/1‑140 (MQ=255)
                                                                                                                   aaGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAAAGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAat                     >  1:3168562/1‑140 (MQ=255)
                                                                                                                    ataattttttttGCTGAATAAGTTAGAATGTTTTTATTATTAACGAATCGCTTATCTTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAatc                    <  2:3810436/133‑1 (MQ=255)
                                                                                                                           ttttgGCTCAATATGGTACATTGTTTTTCTTATGACCGAAGCGCTGATTTTCGGGGTTAAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCt             <  1:1048214/140‑1 (MQ=255)
                                                                                                                             tttGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTg           >  2:6579671/1‑140 (MQ=255)
                                                                                                                             tttGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTg           >  2:7502482/1‑140 (MQ=255)
                                                                                                                                   gcATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCTATCGCTTGCCTGaa     >  1:6884978/1‑140 (MQ=255)
                                                                                                                                   gaatatGGTAGAATTGTTTTATTATGAACGAATGGATTTACTTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGaa     <  2:2987443/138‑1 (MQ=255)
                                                                                                                                    cATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTACAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGaaa    >  1:4327453/1‑140 (MQ=255)
                                                                                                                                     aTAAGTTTGAATTCTTTTCTTATTAACGAATCGCTGATCTTCTCTGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAg   <  2:123433/140‑1 (MQ=255)
                                                                                                                                     aTAAGTTATAATGCTTTTATTATGAACGAATCGATGATGTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAg   <  2:7190221/140‑1 (MQ=255)
                                                                                                                                     aTAAGGTAGAATGATTTTCTTATTAACTAATCGCTGATCTTCTCGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAg   <  2:5580427/140‑1 (MQ=255)
                                                                                                                                      tAAGGTATATTGCTTTTCTTATGACCGAATCGCTTATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGc  <  2:2392534/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                       
CCGAGAATCATCGAATCATTTTCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGC  >  NZ_CP034943/1293168‑1293441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: