Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,582,926 C→A 5.7% I39I (ATC→ATA ylyA → sporulation‑related RNA polymerase‑binding protein YlyA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,582,9260CA 5.7% 964.7 / 2.2 383I39I (ATC→ATAylyAsporulation‑related RNA polymerase‑binding protein YlyA
Reads supporting (aligned to +/- strand):  ref base C (209/134);  new base A (2/37);  total (211/172)
Fisher's exact test for biased strand distribution p-value = 3.14e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 408 total aligned reads displayed.

TAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCTTTCAAAGCTTG  >  NZ_CP034943/1582789‑1583065
                                                                                                                                         |                                                                                                                                           
       aGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATaaa                                                                                                                                    >  2:6302638/1‑140 (MQ=255)
       aGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATaaa                                                                                                                                    >  2:6109306/1‑140 (MQ=255)
       aGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATaaa                                                                                                                                    >  1:1940769/1‑140 (MQ=255)
        ggAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAAc                                                                                                                                   >  2:5351864/1‑140 (MQ=255)
         gAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCTAGCAGCACTCAATCATAAACa                                                                                                                                  >  1:6035427/1‑140 (MQ=255)
         gAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACa                                                                                                                                  <  2:7704600/140‑1 (MQ=255)
         gAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACa                                                                                                                                  >  1:5736387/1‑140 (MQ=255)
         gAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACa                                                                                                                                  >  2:3563892/1‑140 (MQ=255)
              gtgATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCGACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAg                                                                                                                             >  1:2279336/1‑140 (MQ=255)
              gtgATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAg                                                                                                                             >  2:708309/1‑140 (MQ=255)
              gtgATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAg                                                                                                                             >  2:5117960/1‑140 (MQ=255)
              gtgATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAg                                                                                                                             >  2:1322945/1‑140 (MQ=255)
                    aatgaatgaTCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCACGCAGCAATCAATCATAAACAAAAAGCAActc                                                                                                                       >  1:4817297/1‑140 (MQ=255)
                      tgaatgaTCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTg                                                                                                                     >  2:3412548/1‑140 (MQ=255)
                      tgaatgaTCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTg                                                                                                                     >  2:3857391/1‑140 (MQ=255)
                      tgaatgaTCAACTGACCGCAATATATACGGAACATCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTg                                                                                                                     >  2:1205047/1‑140 (MQ=255)
                            ataaaCTGACCGAAATATATACGCAACTTATTCTCACGAAAGAACAATTACAGTGGATTTTATTTTATTTTTCTTGCTTTCAGGCTTCAACACGCCCGCAAGCAGCAATCAATCAAAAACAAAAAGCAACTCTGATCTAt                                                                                                               <  1:3272376/137‑1 (MQ=255)
                            aacAACTCACCGAAATATATACGGAACTTCTTCTGACGAAACAATAATTACAGGCGATATTTTTTGAGTATTCTGGCTTTCAGGATTCAACACCCCCGCAAGCAGCAAACAATAAAAAACAAAACGCAACTCTGATCTAt                                                                                                               <  1:6326336/138‑1 (MQ=255)
                            aTCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTAt                                                                                                               >  2:1900255/1‑140 (MQ=255)
                            aTCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTAt                                                                                                               <  2:5760561/140‑1 (MQ=255)
                                aCTGACAGCAATATATCCGGAACTTCTTCTGACTAAAGAAGAATTACAGTCGATATTTTTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATcaca                                                                                                           <  2:2625304/140‑1 (MQ=255)
                                    aCCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTaa                                                                                                       >  2:3598231/1‑140 (MQ=255)
                                    aCCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTaa                                                                                                       >  2:2241256/1‑140 (MQ=255)
                                    aCCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTaa                                                                                                       >  2:4410421/1‑140 (MQ=255)
                                    aCCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTaa                                                                                                       >  1:1254835/1‑140 (MQ=255)
                                      cGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCACGCAGAAATCAATCATAAACAAAAATCAACTCTGATCTATCACAGTaaat                                                                                                     >  1:4030421/1‑139 (MQ=255)
                                      cGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAaag                                                                                                     <  2:459627/140‑1 (MQ=255)
                                      cGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAaag                                                                                                     >  1:6075827/1‑140 (MQ=255)
                                        aaaaatataCTTAACTTATTCTGACTAAATAATAATTACTGTCTAGTTTTTTTTATTTTTCTTTATTTCTGGCTTAAACAAGCCCGCAAGAAGAAATAAATCATAAACAAAAAGCAACTCTGATCTATCACATTAaagaa                                                                                                   <  1:7153768/136‑1 (MQ=255)
                                        aaaTATATACGTAACTTATTCTGACTAAAGAATAATTACAGTCGTTATTATTTGAGTATTCTTGCTTTCAGGATTCAACACGCCCGCAAGCAGAAAACAATCATAAACAAAAAGCAACTCTGATCTATCACATTAaagaa                                                                                                   <  1:1295047/139‑1 (MQ=255)
                                         aaTATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAaagaag                                                                                                  >  2:159359/1‑140 (MQ=255)
                                              ataCGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCtt                                                                                             >  1:4312241/1‑140 (MQ=255)
                                              ataCGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCtt                                                                                             >  2:1746545/1‑140 (MQ=255)
                                              ataCGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCtt                                                                                             >  1:6739637/1‑140 (MQ=255)
                                                aCGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCa                                                                                           >  1:6999852/1‑140 (MQ=255)
                                                  tcaaCTTCTTCTGACTAAATAATAATTACAGTCGATATTTTTTGATTATTCTTGCTTTCTGGCTTCAACACGCCCGCAAGCAGAAAGCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAg                                                                                         <  1:4618517/138‑1 (MQ=255)
                                                  gtaaCTTCTTCTGACTAAAGAATAATTACAGTCGAGATTTTTTGAGTATTCTTGCTTTCAGGCTTAAACAAGCCCGCAACCAGCAATAAATAATAAAAAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAg                                                                                         <  1:1623699/138‑1 (MQ=255)
                                                  ggAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAg                                                                                         >  1:1529119/1‑140 (MQ=255)
                                                  ggAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAg                                                                                         >  1:7510158/1‑140 (MQ=255)
                                                   gAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTAt                                                                                                               >  1:5335029/1‑117 (MQ=255)
                                                   gAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGa                                                                                        >  2:6658598/1‑140 (MQ=255)
                                                   gAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGa                                                                                        >  2:4130677/1‑140 (MQ=255)
                                                   gAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGa                                                                                        >  2:6817428/1‑140 (MQ=255)
                                                   gAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGa                                                                                        >  2:729826/1‑140 (MQ=255)
                                                     actactTCTGACGAAAGAATAATTACAGGCGAGATTATTTGAGTATTCTTGCTTTCAGCCTTAAACAAGCCCGCAAGCAGCAATCACACATAAACAAACAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACg                                                                                      <  1:3576123/140‑1 (MQ=255)
                                                     aCTTCTTCTTACGAAATAATAATTAAAGTCGAGATTTTTTGAGTATTCTTGCTTTCAGGATTCAACAAGCCCGCAAGCAGAAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACg                                                                                      <  2:3180871/140‑1 (MQ=255)
                                                     aCTTCTTCTGACGAAATATGACTTACAGTCTATATTATGTGCTTATTCTTGGTTTCAGGCTGCAACACCCCCGCACGAACCAATCAATCATAACCAAACCGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACg                                                                                      <  1:494859/140‑1 (MQ=255)
                                                      cttcttCTGACTAAATAATAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGt                                                                                     <  2:4020327/140‑1 (MQ=255)
                                                      cttcttCTGACTAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCACGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGt                                                                                     <  2:2953765/140‑1 (MQ=255)
                                                      cttcttCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGAGGt                                                                                     >  2:6890030/1‑140 (MQ=255)
                                                      attcttcTGACTAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACACGCCCGCAAGCTGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGt                                                                                     <  2:5570708/139‑1 (MQ=255)
                                                       ttcttcTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCAt                                                                                                                                       >  2:7660142/1‑89 (MQ=255)
                                                       ttcttcTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAATGAAGAGCTTCAAGACGtt                                                                                    >  1:3749219/1‑140 (MQ=255)
                                                       ttcttcTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGtt                                                                                    >  2:3454096/1‑140 (MQ=255)
                                                       ttcttcTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGtt                                                                                    >  2:904954/1‑140 (MQ=255)
                                                       ttcttcTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGtt                                                                                    >  2:1525821/1‑140 (MQ=255)
                                                       ttcttcTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATAATAAACAAAAAGCAACTCTGATCTAACACATTAAATAAGATCTTCAACACGtt                                                                                    >  2:938154/1‑140 (MQ=255)
                                                       ttcttcTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAAACATAAACAAAAAGCAACTCTGATCAATCACAGTAAAGAAGAGCTTCAAGACGtt                                                                                    >  2:7486299/1‑140 (MQ=255)
                                                              gACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCg                                                                             >  2:5297006/1‑140 (MQ=255)
                                                                ctaaaTAATAATTACATTCGATATTTTTTTATTATTATTGCTTTCAGGATTCAACAACCCCGCAACCAGCAAACAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCa                                                                           <  1:4570157/138‑1 (MQ=255)
                                                                ctaaaTAAGAATTACAGTCTAGATTATTTTATTATTGTTGCTTTCAGGCTAAAACAACCCCGCAAGAAGAAATCAATAAAAAACAAAACGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCa                                                                           <  1:5931848/138‑1 (MQ=255)
                                                                ctaaaGACGAATTACAGTCGATATTATTTGATTATTCTTGCTTTCTGGCTACAACACGCCCGCAAGCAGCAATAAATAATAAACAAAACGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCa                                                                           <  1:3505643/138‑1 (MQ=255)
                                                                cGAAATAACAATTACAGTCGATATTATTTGAGTATTCTTGCTTTAAGGCTTCAACAAGCCCGCACCCACCAATAAATCATAAAAAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCa                                                                           <  1:3429190/140‑1 (MQ=255)
                                                                   aagaagAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTg                                                                        >  1:1800349/1‑140 (MQ=255)
                                                                    agaagaATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGt                                                                       >  2:5477643/1‑140 (MQ=255)
                                                                         aaTTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCaaa                                                                  >  2:2053449/1‑140 (MQ=255)
                                                                                gTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAaagaa                                                                                                   >  1:5827059/1‑100 (MQ=255)
                                                                                gTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGaaa                                                           >  2:1053085/1‑140 (MQ=255)
                                                                                   gagaTTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACg                                                        >  1:5540181/1‑140 (MQ=255)
                                                                                   gagaTTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACg                                                        >  2:144703/1‑140 (MQ=255)
                                                                                   gagaTTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACg                                                        >  2:802696/1‑140 (MQ=255)
                                                                                   gagaTTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATAGAAAACg                                                        >  2:5975230/1‑140 (MQ=255)
                                                                                    tgaTTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACgg                                                       <  2:784357/139‑1 (MQ=255)
                                                                                    agtttattTGATTATTCTTGCTTTCAGGCTTAAAAACGCCCGCAAGCAGCAATAAATCATAAACAAAACGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACgg                                                       <  1:2648522/137‑1 (MQ=255)
                                                                                      tttattTGATTATTTTTGTTTTGTTTGTTAAACAAGCCCTCAAACAGCAATTAATTAAAAACAAAAAGCAACTCTTATCTATCACATTAAAGAAGAGCTTCATGACGTTCTCCTCGCATTGTCCAAAATCGATAACGGTa                                                     <  1:84992/139‑1 (MQ=255)
                                                                                      atttttTTAGTATTCTTTTTTTCAGGCTTAAACAAGCCCTAAATCAGAAATCATTTATAAACATAAAGCAACTCTTATCTATCACATTAAAGAAGAGCTTCAATACGTTTTTCTATAATTTTCCTAATTCGAAAAGGGTa                                                     <  2:5665836/140‑1 (MQ=255)
                                                                                      attattTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTa                                                     <  2:7413580/140‑1 (MQ=255)
                                                                                      attattTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTa                                                     <  2:4344496/140‑1 (MQ=255)
                                                                                      attattTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTa                                                     <  2:1257674/140‑1 (MQ=255)
                                                                                        tattTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACa                                                   <  2:4982252/140‑1 (MQ=255)
                                                                                             gAGTATTCTTTCTTTCAGGCTTCAACAACCCCGCAAGCAGAAAACACACATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCgg                                              <  2:4879281/140‑1 (MQ=255)
                                                                                                 tttCTTGCTTTCTGGATTCAACAAGCCCGCAAGCAGCAATCAATCATAAAAAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATAc                                          <  1:1100819/139‑1 (MQ=255)
                                                                                                      tGCTTTCAGGATACAACAAGCCCGCAAGCAGCAATCAATAATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCga                                     <  1:7213458/140‑1 (MQ=255)
                                                                                                      tGCTTACAGGCTTCAACACGCCCGCACGCACCAATAAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCga                                     <  1:778677/140‑1 (MQ=255)
                                                                                                       gCTTTCAGGCTACAACAAGCCCGCAAGCAGCAAACAACAAAAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCgaa                                    <  1:3905970/140‑1 (MQ=255)
                                                                                                             agtcTTCACCACGCCCGCACGCTGCAATCAATCATAAACAAACAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACt                              <  1:1990997/137‑1 (MQ=255)
                                                                                                               gCTTCAACAAGCCCGCAAGCAGAAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTgg                            <  2:6509999/140‑1 (MQ=255)
                                                                                                                 ttaaacaaGCCCGCAAGCAGAAATAAATAATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTgggg                          <  1:1340169/137‑1 (MQ=255)
                                                                                                                 ttCAACACGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTgggg                          <  1:3333330/140‑1 (MQ=255)
                                                                                                                 ttCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTgggg                          <  1:7618420/140‑1 (MQ=255)
                                                                                                                 tacaaccaGCCCGCAACCAGCAATCAAACAAAAAAAAAACGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTgggg                          <  1:6792766/138‑1 (MQ=255)
                                                                                                                         gCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCAtt                  >  1:2486534/1‑140 (MQ=255)
                                                                                                                         gCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCAtt                  >  1:297101/1‑140 (MQ=255)
                                                                                                                         gCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCAtt                  >  2:2369731/1‑140 (MQ=255)
                                                                                                                           ccGCAAGCAGCAAACAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTcc                <  1:6600901/140‑1 (MQ=255)
                                                                                                                              cAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCt             >  1:2541873/1‑140 (MQ=255)
                                                                                                                              cAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCt             >  1:3054389/1‑140 (MQ=255)
                                                                                                                              cAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCt             >  1:1731696/1‑140 (MQ=255)
                                                                                                                                 gcagcaATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCTTTc          <  1:904954/140‑1 (MQ=255)
                                                                                                                                        tcaatcaTAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCTTTCAAAGCtt   >  1:5203835/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TAAGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCTTTCAAAGCTTG  >  NZ_CP034943/1582789‑1583065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: