Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 154,410 C→T 77.8% R95C (CGT→TGT)  rpsI → 30S ribosomal protein S9

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943154,4100CT77.8% 1291.9 / inf 632R95C (CGT→TGT) rpsI30S ribosomal protein S9
Reads supporting (aligned to +/- strand):  ref base C (65/75);  new base T (242/247);  total (307/325)
Fisher's exact test for biased strand distribution p-value = 5.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 647 total aligned reads displayed.

ACAACCATTAACTTTGACTGAAACAGCTGGCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAGTTCTCAAAACGTTAATTTGCGTTTTTCAAAGCTCTCGACCTTGT  >  NZ_CP034943/154274‑154549
                                                                                                                                        |                                                                                                                                           
acaacCATTAACTTTGACTGAAACAGCTGGCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACtgtg                                                                                                                                          >  2:822376/1‑140 (MQ=255)
acaacCATTAACTTTGACTGAAACAGCTGGCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACtgtg                                                                                                                                          <  2:5482394/140‑1 (MQ=255)
acaacCATTAACTTTGACTGAAACAGCTGGCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTg                                                                                                                                          <  2:3648678/140‑1 (MQ=255)
     cATTAACTTTGACTGAAACAGCTGGCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAaca                                                                                                                                     >  1:1386056/1‑140 (MQ=255)
             ttGACTGAAACAGCTGGCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAAcg                                                                                                                             <  1:1005230/140‑1 (MQ=255)
              tGACTGAAACAGCTGGCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAActc                                                                                                                            >  2:6067913/1‑138 (MQ=255)
              tGACTGAAACAGCTGGCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAAcgc                                                                                                                            >  2:6307420/1‑140 (MQ=255)
              tGACTGAAACAGCTGGCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAAcgc                                                                                                                            >  2:5713998/1‑140 (MQ=255)
               gACTGAAACAGCTGGCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAAcgcg                                                                                                                           <  2:4910034/140‑1 (MQ=255)
                aCTTAAACAGCTGGCACTTATGCTGTTTTATTAAACGTACATGGAGGCGTCTGATCTGGTCAAGCTGGAGCAATCCGTCATTGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACgcgc                                                                                                                          <  2:4483291/140‑1 (MQ=255)
                  tGAAACAGCTGGCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAg                                                                                                                        <  2:5580559/140‑1 (MQ=255)
                  tGAAACAGCTGGCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAg                                                                                                                        <  2:4753112/140‑1 (MQ=255)
                  tGAAACAGCTGGCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAg                                                                                                                        <  2:1495568/140‑1 (MQ=255)
                  tGAAACAGCTGGCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAg                                                                                                                        <  1:6723736/140‑1 (MQ=255)
                  tGAAACAGCTGGCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCGTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAg                                                                                                                        <  2:6954479/140‑1 (MQ=255)
                  tGAAACAGCTGGCACCTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAg                                                                                                                        <  1:2077472/140‑1 (MQ=255)
                   gAAACAGCTGGCACTTATGATGTTTTAGTAAACGTACATTGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAgg                                                                                                                       <  1:4344803/140‑1 (MQ=255)
                       cAGCTGGCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGtctt                                                                                                                   <  2:6615650/140‑1 (MQ=255)
                       cAGCTGGCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGtctt                                                                                                                   <  2:2097648/140‑1 (MQ=255)
                       cAGCTGGCACTTATGATGTTTTAGTAAAAGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGTTCCAGAATACTGTGCAACACTTAAACGCGCAGGtctt                                                                                                                   <  2:3325556/140‑1 (MQ=255)
                         gCTGGCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAGGtcttct                                                                                                                 <  1:3622186/140‑1 (MQ=255)
                          cTGGCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTg                                                                                                                >  2:2840165/1‑140 (MQ=255)
                            ggCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGAc                                                                                                              >  1:119787/1‑140 (MQ=255)
                               aCTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCg                                                                                                           >  1:4705003/1‑140 (MQ=255)
                                  tATGATGTTTTAGTAAACGTACATGGAGGCGGCTTAGCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACCCTTAAACGCGCAGGTCTTCTGACTCGTGa                                                                                                        >  2:3348875/1‑140 (MQ=255)
                                   atgatgTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGAc                                                                                                       >  1:3762667/1‑140 (MQ=255)
                                     gatgTTTTAGTAAACGTACATGGAGGCGGATTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGc                                                                                                     <  2:2620662/140‑1 (MQ=255)
                                           ttAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTAt                                                                                               >  1:808055/1‑140 (MQ=255)
                                                 aaCGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGa                                                                                         >  1:2984761/1‑140 (MQ=255)
                                                   cGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAAc                                                                                       >  1:4169276/1‑140 (MQ=255)
                                                   cGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAAc                                                                                       >  1:1872168/1‑140 (MQ=255)
                                                         tGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTaaaa                                                                                 >  1:560349/1‑140 (MQ=255)
                                                         tGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTaaaa                                                                                 >  1:1564846/1‑140 (MQ=255)
                                                           gAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTaaaaaa                                                                               >  2:4280708/1‑140 (MQ=255)
                                                           gAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTaaaaaa                                                                               >  2:2716531/1‑140 (MQ=255)
                                                             ggcggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATa                                                                             <  2:5797727/140‑1 (MQ=255)
                                                             ggcggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATa                                                                             >  2:7274255/1‑140 (MQ=255)
                                                              gcggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAaca                                                                                                                                     >  1:6070896/1‑83 (MQ=255)
                                                              gcggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTACACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATAc                                                                            <  2:3086599/140‑1 (MQ=255)
                                                              gcggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACg                                                                                                      <  2:4010454/114‑1 (MQ=255)
                                                              gcggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATAc                                                                            <  1:2840165/140‑1 (MQ=255)
                                                              gcggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATAc                                                                            <  2:6183922/140‑1 (MQ=255)
                                                              gcggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATAc                                                                            <  1:4297450/140‑1 (MQ=255)
                                                              gcggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATAc                                                                            <  2:1720867/140‑1 (MQ=255)
                                                              gcggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATAc                                                                            <  2:6770662/140‑1 (MQ=255)
                                                              gcggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATAc                                                                            <  2:460750/140‑1 (MQ=255)
                                                              gcggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATAc                                                                            >  1:6226180/1‑140 (MQ=255)
                                                              gcggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATAc                                                                            <  1:593015/140‑1 (MQ=255)
                                                              gcggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATAc                                                                            <  1:5169685/140‑1 (MQ=255)
                                                              gcggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATAc                                                                            <  2:418049/140‑1 (MQ=255)
                                                              gcggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATAc                                                                            <  1:5258933/140‑1 (MQ=255)
                                                              gcggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATAc                                                                            <  1:639658/140‑1 (MQ=255)
                                                              gcggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATAc                                                                            <  1:5755724/140‑1 (MQ=255)
                                                              gcggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATAc                                                                            <  2:6453984/140‑1 (MQ=255)
                                                              gaggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATAc                                                                            <  1:3042031/138‑1 (MQ=255)
                                                                ggcTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGa                                                                                                               >  1:7398412/1‑103 (MQ=255)
                                                                     aTCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTa                                                                     <  1:7254851/140‑1 (MQ=255)
                                                                      tCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTaa                                                                    >  1:5039074/1‑140 (MQ=255)
                                                                      tCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTaa                                                                    >  1:6572866/1‑140 (MQ=255)
                                                                       cTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTaaa                                                                   <  2:5112797/140‑1 (MQ=255)
                                                                       cTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTaaa                                                                   <  2:7451594/140‑1 (MQ=255)
                                                                       cTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTaaa                                                                   <  2:5423761/140‑1 (MQ=255)
                                                                         ggTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAgg                                                                 <  2:3771776/140‑1 (MQ=255)
                                                                         ggTAAAGCTGGAGCAATCCGTCTTGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAAAACTTAAACGCGCAGGACTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAgg                                                                 <  2:2352662/140‑1 (MQ=255)
                                                                          gTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGc                                                                                                     <  2:4994299/103‑1 (MQ=255)
                                                                            cAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGgcgc                                                              <  1:2748665/140‑1 (MQ=255)
                                                                            cAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGATCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGgcgc                                                              <  2:1374196/140‑1 (MQ=255)
                                                                               gCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCtcg                                                           <  2:3541010/140‑1 (MQ=255)
                                                                               gCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCtcg                                                           <  2:1000987/140‑1 (MQ=255)
                                                                                    aGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATAc                                                                            <  1:3372195/118‑1 (MQ=255)
                                                                                    aGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTg                                                      >  1:6819854/1‑140 (MQ=255)
                                                                                         tCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAACGAACGTACAAACTACGGTCTTAAAGGCGCTCGTCGTGCACCt                                                 >  1:2216521/1‑140 (MQ=255)
                                                                                         tCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCt                                                 >  2:7703273/1‑140 (MQ=255)
                                                                                         tCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCt                                                 >  1:1313457/1‑140 (MQ=255)
                                                                                         tCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCt                                                 >  1:485948/1‑140 (MQ=255)
                                                                                         tCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCt                                                 >  1:890488/1‑140 (MQ=255)
                                                                                         tCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCt                                                 >  1:1931727/1‑140 (MQ=255)
                                                                                         tCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCt                                                 >  1:6147122/1‑140 (MQ=255)
                                                                                           cGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCa                                               >  2:522732/1‑140 (MQ=255)
                                                                                            gTCATGGTATCGATCGTGCTTTGCTTGAAGCAGATCCAGAATACTGAGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGACCGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAg                                              <  2:3883538/140‑1 (MQ=255)
                                                                                              cATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAGtt                                            <  2:2528032/140‑1 (MQ=255)
                                                                                                   tATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAGTTCTCaa                                       >  1:4949905/1‑140 (MQ=255)
                                                                                                            tgctttgcttGAAGCAGATCCAGAATACTGTGCAACACTTAAAcgcg                                                                                                                           <  2:2240458/47‑1 (MQ=255)
                                                                                                            tgctttgcttGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAGTTCTCAAAACGTTAAt                              >  1:4437322/1‑140 (MQ=255)
                                                                                                             gctttgcttGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAGTTCTCAAAACGTTAAtt                             >  1:4584964/1‑140 (MQ=255)
                                                                                                                 tgcttGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCGGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAGTTCTCAAAACGTTAATTTGCg                         <  1:3280163/140‑1 (MQ=255)
                                                                                                                   cttGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAGTTCTCAAAACGTTAATTTGCGtt                       >  2:7315003/1‑140 (MQ=255)
                                                                                                                   cttGAAGCAGATCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAGTTCTCAAAACGTTAATTTGCGtt                       >  1:6905909/1‑140 (MQ=255)
                                                                                                                       aaGCAGATCCAGAATACTGTGCAACACTTAAACGCGCCGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAGTTCTCAAAACGTTAATTTGCGttttta                   >  1:2416585/1‑139 (MQ=255)
                                                                                                                        aGCAGATCCAGAATACTGTGCAACACTTAACCGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAGTTCTCAAAACGTTAATTTGCGTTTTTCa                  <  1:4030206/140‑1 (MQ=255)
                                                                                                                            caTCCAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAGTTCTCAAAACGTTAATTTGCGTTTTTCAAAGc              <  1:5410061/139‑1 (MQ=255)
                                                                                                                             aTCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAGTTCTCAAAACGTTAATTTGCGTTTTTCa                  <  2:324328/135‑1 (MQ=255)
                                                                                                                             aTCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAGTTCTCAAAACGTTAATTTGCGTTTTTCAAAGct             >  1:3957640/1‑140 (MQ=255)
                                                                                                                             aTCCAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAGTTCTCAAAACGTTAATTTGCGTTTTTCAAAGct             >  1:2641353/1‑140 (MQ=255)
                                                                                                                             aTCCAGAATACTGTGCAACACTTAAACGCGCAGGGCTGCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAGTTCTCAAAACGTTAATTTGCGTTTTTCAAAGct             >  1:3488305/1‑140 (MQ=255)
                                                                                                                               ccAGAATACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCATTTCTCAAAACGTTAATTTGCGTTTTTCAAAGctct           <  1:7661189/140‑1 (MQ=255)
                                                                                                                               ccAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAGTTCTCAAAACGTTAATTTGCGTTTTTCAAAGctct           <  1:7088313/140‑1 (MQ=255)
                                                                                                                                   aaTACTGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAGTTCTCAAAACGTTAATTTGCGTTTTTCAAAGCTCTCGAc       <  1:7297717/140‑1 (MQ=255)
                                                                                                                                        cGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAGTTCTCAAAACGTTAATTTGCGTTTTTCAAAGCTCTCGACCTtgt  >  2:7196202/1‑140 (MQ=255)
                                                                                                                                        cGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAGTTCTCAAAACGTTAATTTGCGTTTTTCAAAGCTCTCGACCTtgt  >  2:1148951/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                           
ACAACCATTAACTTTGACTGAAACAGCTGGCACTTATGATGTTTTAGTAAACGTACATGGAGGCGGCTTATCTGGTCAAGCTGGAGCAATCCGTCATGGTATCGCTCGTGCTTTGCTTGAAGCAGATCCAGAATACCGTGCAACACTTAAACGCGCAGGTCTTCTGACTCGTGACGCTCGTATGAAAGAACGTAAAAAATACGGTCTTAAAGGCGCTCGTCGTGCACCTCAGTTCTCAAAACGTTAATTTGCGTTTTTCAAAGCTCTCGACCTTGT  >  NZ_CP034943/154274‑154549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: