Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,925,447 C→A 7.5% R1870L (CGA→CTA)  EO946_RS09730 ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,925,4470CA 7.5% 946.7 / 6.3 330R1870L (CGA→CTA) EO946_RS09730non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base C (61/220);  new base A (24/2);  total (108/222)
Fisher's exact test for biased strand distribution p-value = 5.81e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 333 total aligned reads displayed.

CTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTGGCCCTTA  >  NZ_CP034943/1925308‑1925585
                                                                                                                                           |                                                                                                                                          
cTCAAGATTAGAGCGCTCCTTGTGATAATACTTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCTATCGCTGCTGAAGATGTCTCTGTGTCAAGACAATTTGTGTTTTAGAGTCGTTTAATATATATTGTATTc                                                                                                                                            <  1:1453583/140‑1 (MQ=255)
cTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACACTTTCTGTTTTAGAGTCGTGTAATATAAATTTTATTc                                                                                                                                            >  1:2432960/1‑140 (MQ=255)
 tCAAGCGTAGAGCGCGCCTTGTGATAAGAATTGTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                                           <  1:7302588/140‑1 (MQ=255)
 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                                           <  1:6694043/140‑1 (MQ=255)
 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                                           <  1:6529121/140‑1 (MQ=255)
 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                                           <  2:7007544/140‑1 (MQ=255)
 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                                           <  1:7563045/140‑1 (MQ=255)
 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                                           <  2:1215854/140‑1 (MQ=255)
 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                                           <  2:6910895/140‑1 (MQ=255)
 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                                           <  1:6466987/140‑1 (MQ=255)
 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                                           <  1:2865085/140‑1 (MQ=255)
 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                                           <  1:5139556/140‑1 (MQ=255)
 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                                           <  1:4406209/140‑1 (MQ=255)
 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                                           <  2:1839406/140‑1 (MQ=255)
 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                                           <  2:332730/140‑1 (MQ=255)
 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                                           <  1:1716863/140‑1 (MQ=255)
 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                                           <  2:2351608/140‑1 (MQ=255)
 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                                           <  2:5720899/140‑1 (MQ=255)
 tCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCAGCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                                           <  2:5851780/140‑1 (MQ=255)
 tCAAGATTAGAGCGCTACTTGTGATAAGAATTGTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                                           <  1:4800571/140‑1 (MQ=255)
 gccaGATTAGAGCGCTCCTTGTGATAAGAATGTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCg                                                                                                                                           <  1:6158336/137‑1 (MQ=255)
      aTTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCgctgct                                                                                                                                      <  2:7600369/140‑1 (MQ=255)
      aTTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCgctgct                                                                                                                                      <  1:4603467/140‑1 (MQ=255)
           agCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGa                                                                                                                                 <  2:140968/140‑1 (MQ=255)
           agCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGa                                                                                                                                 <  1:1583012/140‑1 (MQ=255)
             cgcTCCTTGTGATACGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTAAAAGATATTGTATTTGCTGctcttggta                                                                                                                               >  1:4132763/1‑135 (MQ=255)
             cgcTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTAAGATATATTTTATTAGCGGCTCTGGGTa                                                                                                                               >  1:3217002/1‑140 (MQ=255)
               cTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTTATATATAATGTATTAGCTGCTGTGGATAGt                                                                                                                             >  2:5620912/1‑140 (MQ=255)
               cTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTGATATATATTTTATTAGGTGctcttgtgatt                                                                                                                             >  1:6321698/1‑133 (MQ=255)
               cTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAAGTTCTGTTTTAGAGTCGTGTAAGAAATATGTTATTCGCTGGTCTgtctctg                                                                                                                             >  1:2206210/1‑134 (MQ=255)
                tCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTc                                                                                                                            <  1:4840803/140‑1 (MQ=255)
                   ttGTTATAAGAATTTTCATCATCCAATAGAACGATCGTGCATTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGTGTCGTTTAATATTTTTTTTTTTATCTGATCTGGATATTcgtg                                                                                                                         <  1:2282899/140‑4 (MQ=255)
                       gATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGGGATATAAATTGTTTTTGCTGGGATGGATATTCCgggtaa                                                                                                                     >  1:3555548/1‑136 (MQ=255)
                            gAATTTTCATCCTCCAAGAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATGTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAgg                                                                                                                <  2:2925101/140‑1 (MQ=255)
                                           aaTAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCgccgcc                                                                                                 <  2:4151165/140‑1 (MQ=255)
                                           aaTAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATAGTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCgccgcc                                                                                                 <  2:5535436/140‑1 (MQ=255)
                                            aTAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGccgccg                                                                                                <  2:3591355/140‑1 (MQ=255)
                                            aTAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGccgccg                                                                                                <  2:3430022/140‑1 (MQ=255)
                                                  cGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTTATATATATTTTTTTTGCTGGTGTGGCTGGTCCGGGGGGATAGGCCCCGAGGCGCCGCCGGcggtt                                                                                          >  1:6955366/1‑136 (MQ=255)
                                                     tCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAAt                                                                                       <  2:932726/140‑1 (MQ=255)
                                                     tCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTGAATATATATTTTTTTCGCTGGGCGGGATAGTGCGGATCAATAGGCACCTTGGCGCCCCCGGCGTTTaaa                                                                                       >  2:6489618/1‑139 (MQ=255)
                                                          ccTTCATGGGCCAATAGCTGCTGAAGATGTCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATTTATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGc                                                                                  <  2:905381/140‑1 (MQ=255)
                                                           cTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTAAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGcc                                                                                 <  2:7337033/140‑1 (MQ=255)
                                                               aTGGTCCAATCGATTCTGTAGATGTCTCTGTGTCAAGACAATTTCTTTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGaa                                                                             <  2:7038173/140‑1 (MQ=255)
                                                               aTGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTTATATATATTTTTTTGGGTGGTCTGGGTAGTACGGTTCAAAATGGACGTAGGCGCCCCCCGCGTTTAATAAGGCCACaa                                                                             >  1:1766974/1‑140 (MQ=255)
                                                                         cGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAAc                                                                   <  2:4827391/140‑1 (MQ=255)
                                                                         cGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAAc                                                                   <  1:1363462/140‑1 (MQ=255)
                                                                         cGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAAc                                                                   <  2:59998/140‑1 (MQ=255)
                                                                         cGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAAc                                                                   <  2:3897184/140‑1 (MQ=255)
                                                                         cGCTGCGGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGGATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAAc                                                                   <  2:3822815/140‑1 (MQ=255)
                                                                           ctgctgAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCa                                                                 <  2:4973574/140‑1 (MQ=255)
                                                                             gctgAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTGTTAAATATATTGTATTAGCGGCTCTGGGTAATACCGATAAAAAGGCACGGAGGCGCCCCCGGCTTTTAATAAAGCCCGAAATAACACAACCact                                                               >  2:3078649/1‑138 (MQ=255)
                                                                              ctgAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGtt                                                              <  2:2787007/140‑1 (MQ=255)
                                                                              ctgAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGtt                                                              <  2:1642404/140‑1 (MQ=255)
                                                                                        ctctGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCtgtg                                                    <  1:6780435/140‑1 (MQ=255)
                                                                                            gtgtCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCg                                                <  2:1084980/140‑1 (MQ=255)
                                                                                            gtgtCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCg                                                <  1:3311212/140‑1 (MQ=255)
                                                                                              gtCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCt                                              <  1:707798/140‑1 (MQ=255)
                                                                                                               tttAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTc                             <  1:2770185/140‑1 (MQ=255)
                                                                                                               tttAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGATTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTc                             <  1:3777963/140‑1 (MQ=255)
                                                                                                                   tagTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCTCGTAGGCGCCGGCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGTTGCTTTTTTCGATTTTGATAGCTGCGGttgtttg                         <  1:5642376/139‑6 (MQ=255)
                                                                                                                   gagTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTc                         <  2:6249154/140‑1 (MQ=255)
                                                                                                                           tAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCa                 <  1:935674/140‑1 (MQ=255)
                                                                                                                           tAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCa                 <  1:3002721/140‑1 (MQ=255)
                                                                                                                           tAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCa                 <  2:435730/140‑1 (MQ=255)
                                                                                                                           tAATATATATTGTAGTCGCTGCTCAGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCAGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGGTGTGGTCGCTGATGAGAGCTGCGGTTCGGTCCGACCTCa                 <  2:1898816/140‑1 (MQ=255)
                                                                                                                                  tatTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCtt          <  1:1199619/140‑1 (MQ=255)
                                                                                                                                  tatTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCtt          <  2:5126239/140‑1 (MQ=255)
                                                                                                                                  tatTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCtt          <  2:2251086/140‑1 (MQ=255)
                                                                                                                                  tatTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCtt          <  1:3404723/140‑1 (MQ=255)
                                                                                                                                  tatTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCtt          <  1:5941989/140‑1 (MQ=255)
                                                                                                                                   atTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTg         <  1:1129649/140‑1 (MQ=255)
                                                                                                                                   atTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTg         >  2:2637134/1‑140 (MQ=255)
                                                                                                                                   atTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTg         >  2:714719/1‑140 (MQ=255)
                                                                                                                                   atTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTg         <  1:3910452/140‑1 (MQ=255)
                                                                                                                                   atTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTg         >  1:4020662/1‑140 (MQ=255)
                                                                                                                                   atTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTg         >  1:5776179/1‑140 (MQ=255)
                                                                                                                                   atTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTg         >  1:6098290/1‑140 (MQ=255)
                                                                                                                                   atTGTATTCGCTGCTCTGGATAGTCCGGATCAAAAGGCACGTAGGCGCCCCCGGCTTTTATTAAGGCCCGAATTCCCACAACCAGTGCGATGCTGTGGTCGCTGATCATAGCTGCCGTTCGGGCCGACCTCTCTCCCtgg         >  2:4505842/1‑138 (MQ=255)
                                                                                                                                    ttGTATTCGCTGCTCTTGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTgg        <  1:3686474/140‑1 (MQ=255)
                                                                                                                                     tGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTGGc       <  1:4733740/140‑1 (MQ=255)
                                                                                                                                       tATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGTGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTGGccc     >  1:3508467/1‑140 (MQ=255)
                                                                                                                                       tATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCg                                                <  1:7236855/97‑1 (MQ=255)
                                                                                                                                       tATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTGGccc     >  1:1966141/1‑140 (MQ=255)
                                                                                                                                       tATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTGGccc     >  1:2589060/1‑140 (MQ=255)
                                                                                                                                       tATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTGGccc     >  2:1259189/1‑140 (MQ=255)
                                                                                                                                       tATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTGGccc     >  1:5043392/1‑140 (MQ=255)
                                                                                                                                       tATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTGGccc     >  1:418779/1‑140 (MQ=255)
                                                                                                                                       tATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTGGccc     >  1:7711514/1‑140 (MQ=255)
                                                                                                                                       tATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGAGGTTCGGTCCGACCTCACTCCCTTGGccc     >  2:6639933/1‑140 (MQ=255)
                                                                                                                                        aTTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTGGCCCt    >  2:1945013/1‑140 (MQ=255)
                                                                                                                                         ttCGCTGCTCTGGATAGTCCGGCTCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCCGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATCATAGCTGCGGTTCGGTCCGACCTCACTCCCTTGGCCCtt   >  2:7217050/1‑140 (MQ=255)
                                                                                                                                         ttCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCtt          >  2:4924161/1‑133 (MQ=255)
                                                                                                                                         ttCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTGGCCCtt   <  2:6710054/140‑1 (MQ=255)
                                                                                                                                         ttCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTGGCCCtt   >  2:1528485/1‑140 (MQ=255)
                                                                                                                                         ttCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTGGCCCtt   >  1:2347511/1‑140 (MQ=255)
                                                                                                                                         ttCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTGGCCCtt   >  1:7437508/1‑140 (MQ=255)
                                                                                                                                         ttCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGGTCGATGCTGTGGTCGCTGATGATAGATGCGGTTCGGTCCGACCTCACTCCCTTTGCCCt  >  1:774241/1‑139 (MQ=255)
                                                                                                                                          tCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCtt          <  1:5580422/132‑1 (MQ=255)
                                                                                                                                          tCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTGGCCCTTa  <  1:5369954/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CTCAAGATTAGAGCGCTCCTTGTGATAAGAATTTTCATCATCCAATAGAACGATCGTGCCTTCATGGGCCAATCGCTGCTGAAGATGGCTCTGTGTCAAGACAATTTCTGTTTTAGAGTCGTTTAATATATATTGTATTCGCTGCTCTGGATAGTCCGGATCAATAGGCACGTAGGCGCCGCCGGCTTTTAATACGGCCAGAATTCCCACAACCAGTTCGATGCTGTGGTCGCTGATGATAGCTGCGGTTCGGTCCGACCTCACTCCCTTGGCCCTTA  >  NZ_CP034943/1925308‑1925585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: