Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,034,593 C→A 18.4% intergenic (‑259/‑135) EO946_RS10175 ← / → EO946_RS10180 UPF0715 family protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,034,5930CA18.4% 437.6 / 40.3 214intergenic (‑259/‑135)EO946_RS10175/EO946_RS10180UPF0715 family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (151/17);  new base A (2/44);  total (153/61)
Fisher's exact test for biased strand distribution p-value = 4.04e-29
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

AATGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGTGACACCAATGA  >  NZ_CP034943/2034457‑2034731
                                                                                                                                        |                                                                                                                                          
aaTGGTGGCTGTTATGTGTACATGCAGATTCATTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCaaa                                                                                                                                         >  2:407133/1‑140 (MQ=255)
     tggCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGa                                                                                                                                    >  2:638333/1‑140 (MQ=255)
      ggtttttATTTTTAAATGCATTTTCCTTCCATAACCGGTCCTTATCTCCGATTATTTTTAAAACGTTTTGTACAGTTTTCCCCAGATTTCGTCAGCTTCTTCATATACGACGTGTGCTAATAAAATGTTTCAAACACGAc                                                                                                                                   <  2:4331772/135‑1 (MQ=255)
       gCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTTTAGAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGccc                                                                                                                                  >  2:6124775/1‑140 (MQ=255)
       gCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGAcc                                                                                                                                  >  1:5388750/1‑140 (MQ=255)
       gCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGAcc                                                                                                                                  >  2:5936180/1‑140 (MQ=255)
           ttATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTGATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTGAGCTTCTTCATATACGAAGTGTGCTAATAGAATGTTTCAAAAACTACCgggg                                                                                                                              >  2:4834615/1‑140 (MQ=255)
                 ttACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGc                                                                                                                        >  1:5499299/1‑140 (MQ=255)
                     aTGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGAc                                                                                                                    >  2:6737537/1‑140 (MQ=255)
                        cAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGActct                                                                                                                 >  2:2404425/1‑140 (MQ=255)
                        cAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTGTCAAAAACGACCGGGGTAAAGCTGActct                                                                                                                 >  2:2924355/1‑140 (MQ=255)
                         cgagtccttccCTACCCTGTCCTTAGCTCGGTTTTTTTTTAAAAGTTTTTGTACCGTTTTTCCCAGCTTTAGTCAGCTTTTTCATATACGAAGTGTGCTAATAAATTTTTTCAAACACGACCGGGGTAAAGCTGActctc                                                                                                                <  2:58239/136‑1 (MQ=255)
                          gATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGActctct                                                                                                               >  1:2758355/1‑140 (MQ=255)
                          gATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGActctct                                                                                                               >  1:1790323/1‑140 (MQ=255)
                          gATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGActctct                                                                                                               >  1:3506563/1‑140 (MQ=255)
                          gATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAAAGTTTTGTACAGTGTTTCCCAGCTTTAGTAAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGActctct                                                                                                               >  1:2138595/1‑140 (MQ=255)
                          gATTCCTTCCCTAACCTGTCATGATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGActctct                                                                                                               >  1:6408999/1‑140 (MQ=255)
                           aTTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCtt                                                                                                              >  2:7313534/1‑140 (MQ=255)
                               cttccCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCt                                                                                                          >  1:2090639/1‑140 (MQ=255)
                                  cccTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTaa                                                                                                       >  1:123129/1‑140 (MQ=255)
                                       aCCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGa                                                                                                  >  2:1677813/1‑140 (MQ=255)
                                       aCCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGa                                                                                                  >  1:604335/1‑140 (MQ=255)
                                       aCCTGTCCTGATACCCGCTTTTTTTTAAAAGTTTTTTTAATTTGTTTCCCAGTTTTATTCTGATTCTTAATATCCGACTGTTGCTAATAAAATGTTTCAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGa                                                                                                  <  2:2333253/140‑1 (MQ=255)
                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACTAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  2:5906616/1‑140 (MQ=255)
                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  2:2598848/1‑140 (MQ=255)
                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  2:286236/1‑140 (MQ=255)
                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  2:3129872/1‑140 (MQ=255)
                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  2:116503/1‑140 (MQ=255)
                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  2:6566558/1‑140 (MQ=255)
                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  1:944062/1‑140 (MQ=255)
                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  1:7676492/1‑140 (MQ=255)
                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  1:7363302/1‑140 (MQ=255)
                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  2:91481/1‑140 (MQ=255)
                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  2:1430671/1‑140 (MQ=255)
                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  1:4067164/1‑140 (MQ=255)
                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  1:4925741/1‑140 (MQ=255)
                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  1:2455409/1‑140 (MQ=255)
                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  1:2205209/1‑140 (MQ=255)
                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCGAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  1:2833717/1‑140 (MQ=255)
                                             ccTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTGCCTATACGAAGTGTGCTAAGCAAATGTTTCAAAAACGACCGGGGTTAAGCTGACTCTCTTGTCTAAATGGGAAAGAGt                                                                                            >  2:2448365/1‑140 (MQ=255)
                                                 aTCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCCGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAATCGACCGGGGTCAAGCTGACTCTCTTGTCTTCATGGGAAAGAGTCAAc                                                                                        >  1:779545/1‑140 (MQ=255)
                                                 aTCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCGTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAAGGGGCAAGAGTAAAc                                                                                        >  1:4409235/1‑140 (MQ=255)
                                                      cGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGaaaa                                                                                   >  1:7123589/1‑140 (MQ=255)
                                                      cGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGaaaa                                                                                   >  2:2590850/1‑140 (MQ=255)
                                                      cGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGaaaa                                                                                   >  2:5499065/1‑140 (MQ=255)
                                                      cGCTTCTTTTCCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGaaaa                                                                                   >  1:962442/1‑140 (MQ=255)
                                                                     gtttttGTACTGTGTTTCCCAGCTTTATTCAGCTTATTAATATACGAATTTTGCTAATAAAATTTTTAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATAc                                                                    <  2:5606993/138‑1 (MQ=255)
                                                                     ctttttGTACTGTGTTCCCCACCTTAATTCAGATAATAATCTTACGAAGTGTCATAATAAAATGTTCAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATAc                                                                    <  2:1793617/138‑1 (MQ=255)
                                                                             actttgtTTCCCAGATTTCTTCAGATTCTTCATATACGATTTGTGCTAATAAAATGTTTAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTaa                                                            <  2:7676492/136‑1 (MQ=255)
                                                                                 tttttCCCAGCTTTAGTCAGCTTCTTAATAGCCGAAGTGTGATAATAAAATGTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacaga                                                        <  1:5428870/138‑1 (MQ=255)
                                                                                 tttttCCCAGATTTAGTCAGCTTCTTAATATACGATTTGTGATAAAAAATTTTTTAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacaga                                                        <  2:2709997/138‑1 (MQ=255)
                                                                                 tgtTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacaga                                                        >  2:4139250/1‑140 (MQ=255)
                                                                                 tgtTTCCAATATTTATTCTGCTTATTCATATACGATTTGTTATAATAAATTGTTAAAAAAACGAACGGGGTAAATCTGACTCTCTTGTCTTAATTGGAAAGAGTCTACGAAAAAAAGCGAATGAATACCTGCCTAacaga                                                        <  2:1085667/140‑1 (MQ=255)
                                                                                  ttttCCCTGCTTTATTCTGTTTATTCATTAACCACTTGTGAAAATAAATTTTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacagac                                                       <  1:1116213/139‑1 (MQ=255)
                                                                                  ttttCCCTGCTTTAGTCAGCTTATTCATATACTATGTGTGCTAATAAATTTTTTAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacagac                                                       <  2:7051125/139‑1 (MQ=255)
                                                                                  ttttCCAATCTTTATACAGCTTATTAATATACTAAGTGTTCAAATAAAATTTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacagac                                                       <  2:6271549/139‑1 (MQ=255)
                                                                                  gtTCCCCAGCTTTCGTCCGATTTTTAATATACTACTTGTGCTAAAAAATTTTTTAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacagac                                                       <  2:7655943/140‑1 (MQ=255)
                                                                                      cccAGCTTTAGTCAGCTTCTTCATATACGACGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGaa                                                   >  1:456309/1‑140 (MQ=255)
                                                                                      cccAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGaa                                                   >  2:6099861/1‑140 (MQ=255)
                                                                                       ccAGATTTAGACAGATTCTTTATCTCCCATGTGTGATAATAAATTGTATCATAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGCAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAAt                                                  <  2:5537804/140‑1 (MQ=255)
                                                                                        cAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATa                                                 >  1:1042842/1‑140 (MQ=255)
                                                                                        cAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATa                                                 >  1:2392665/1‑140 (MQ=255)
                                                                                           ttttCGTAAGCTTCTTCATATAGTACGTGTGATAATAAAATGTTAAAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaaga                                              <  2:3981471/139‑1 (MQ=255)
                                                                                           cTTTTGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACTGACAGAATaaga                                              >  1:4261370/1‑140 (MQ=255)
                                                                                           cTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaaga                                              >  2:355293/1‑140 (MQ=255)
                                                                                           cTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaaga                                              >  2:1567407/1‑140 (MQ=255)
                                                                                           cTTTAGTCAGCTTCTTCATATACGAAGTGGGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaaga                                              >  2:1308469/1‑140 (MQ=255)
                                                                                                gTCAGTTTCTTCATATAGTTTGTTTTATAAAAAATGGTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGt                                         <  1:6959359/140‑1 (MQ=255)
                                                                                                  aaGCTTCTTCATATACTCCTTGTGATAATAAATTGTTTAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTg                                       <  2:7123589/139‑1 (MQ=255)
                                                                                                     cttcttCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACATACAGAATAAGAATAGTTGata                                    >  1:1566365/1‑140 (MQ=255)
                                                                                                     cttcttCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGata                                    >  1:5279009/1‑140 (MQ=255)
                                                                                                           cATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAac                              >  1:3633767/1‑140 (MQ=255)
                                                                                                              ataCGATGTGTTCTAATAAATTGTTACAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACt                           <  2:5279009/140‑1 (MQ=255)
                                                                                                              aacccaaGTGTCCTAAAAACATTATACAAACACGACCGGGCTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACt                           <  1:5150555/135‑1 (MQ=255)
                                                                                                               taCGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAacacct                          >  1:5930117/1‑138 (MQ=255)
                                                                                                                   aaGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCg                      >  2:3381035/1‑140 (MQ=255)
                                                                                                                   aaGTGTGCTAATAAAATGTTTAAAAAACGAACGGGGTAAAGCTTACTCTCTTGTCTTAATGTGAAAGAGTCAACGAAAAAAATCGAATGAAAAACTGCATAAAAGAAATAATAAGAAGAGTTGATATTCAACAATTTTCg                      >  2:199013/1‑140 (MQ=255)
                                                                                                                     gtgtGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACCGAATAAGAAGAGTTGATATTCAACACTTTTCGTa                    >  2:4689659/1‑140 (MQ=255)
                                                                                                                      ttttcTAATAATATTTTAAAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTaa                   <  1:5864898/136‑1 (MQ=255)
                                                                                                                      tgtgCTAATAAAATTTTGAAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTaa                   <  2:2222210/140‑1 (MQ=255)
                                                                                                                      tgtgATAATAAATTTTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTaa                   <  2:782911/140‑1 (MQ=255)
                                                                                                                        tgCTAATAAAATGTTTCAAAAACGACGGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAACAGCGTATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCCACACTTTTCGTAAAg                 >  1:3776211/1‑140 (MQ=255)
                                                                                                                        tgCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacaga                                                        >  1:880227/1‑101 (MQ=255)
                                                                                                                        tgCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAg                 >  2:1327106/1‑140 (MQ=255)
                                                                                                                        tgCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAg                 >  1:2732170/1‑140 (MQ=255)
                                                                                                                        tgCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAg                 >  2:372145/1‑140 (MQ=255)
                                                                                                                        tgCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAg                 >  1:6318479/1‑140 (MQ=255)
                                                                                                                        tgCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAAAAATAAGAGTTGATATTCAACACTTTTCGTAAAg                 >  2:1121377/1‑140 (MQ=255)
                                                                                                                        tgCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAACGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAg                 >  1:3232545/1‑140 (MQ=255)
                                                                                                                         gataaaaaATTGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAgg                <  2:3525986/138‑1 (MQ=255)
                                                                                                                          cTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAggg               >  1:5974793/1‑140 (MQ=255)
                                                                                                                           taataaATTTTTTAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAgggg              <  2:7393395/140‑1 (MQ=255)
                                                                                                                           taataaATTTTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAgggg              <  1:6506730/140‑1 (MQ=255)
                                                                                                                           taataaACTGTCTCAAAAACGACCGGGGTAAATCTGACTCTCTTGTCTTAATGGTAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAgggg              <  2:7336993/140‑1 (MQ=255)
                                                                                                                           aaaaaaatTGTTAAAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAgggg              <  2:604335/136‑1 (MQ=255)
                                                                                                                            aataaAATGTTACAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGt             <  1:5646145/140‑1 (MQ=255)
                                                                                                                             ataaAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGTg            >  2:6453972/1‑140 (MQ=255)
                                                                                                                                aaaTGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGTGaca         >  1:1788618/1‑140 (MQ=255)
                                                                                                                                  aTGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGTGACAcc       >  1:3946604/1‑140 (MQ=255)
                                                                                                                                       tCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGTGACACCAATGa  >  2:5135318/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                          
AATGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGTGACACCAATGA  >  NZ_CP034943/2034457‑2034731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: