Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,103,774 C→A 12.5% V4L (GTA→TTA)  azoJ ← FMN‑dependent NADH‑azoreductase AzoJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,103,7740CA12.5% 442.4 / 12.7 192V4L (GTA→TTA) azoJFMN‑dependent NADH‑azoreductase AzoJ
Reads supporting (aligned to +/- strand):  ref base C (39/119);  new base A (30/3);  total (70/122)
Fisher's exact test for biased strand distribution p-value = 8.39e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 195 total aligned reads displayed.

AAGGTTCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATC  >  NZ_CP034943/2103637‑2103913
                                                                                                                                         |                                                                                                                                           
aaggTTCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTg                                                                                                                                           <  1:4773395/140‑1 (MQ=255)
aaggTTCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTg                                                                                                                                           <  1:440572/140‑1 (MQ=255)
  ggTTCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTa                                                                                                                                         <  2:6648513/140‑1 (MQ=255)
  ggTTCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTa                                                                                                                                         <  2:305574/140‑1 (MQ=255)
  ggTTCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTa                                                                                                                                         <  1:3454840/140‑1 (MQ=255)
  ggTTCTCTTTATGAAGATCTAATTCAACCAATTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTa                                                                                                                                         <  1:5018602/140‑1 (MQ=255)
  ggTTCTCGTTATGAAGATCTAATTCAACCACTTCATCCGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGATGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTa                                                                                                                                         <  1:6468086/140‑1 (MQ=255)
  ggTTCTATTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTa                                                                                                                                         <  1:7515543/140‑1 (MQ=255)
    ttCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGa                                                                                                                                       <  1:1662191/140‑1 (MQ=255)
    ttCTCTTTATGAAGATCTAATTCAACCACGTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGa                                                                                                                                       <  2:1661931/140‑1 (MQ=255)
    ttCTCTTTATGAAGATCGAATTCCACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGa                                                                                                                                       <  2:6686423/140‑1 (MQ=255)
     tctcTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGAc                                                                                                                                      >  2:5837568/1‑140 (MQ=255)
     tctcTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATAATGTAGaa                                                                                                                                      >  2:4858801/1‑139 (MQ=255)
     actctTTATGAAGATCAAATACAACAACTCAATCAGCACCGTTATTTTCATTATAAGAAGCTAATAAAGCCTCGTAAAGTTTACTAGAAACGCCTACTTCGGCTGTACGCTAGATTGATTTTACAAATAATACTGTATAc                                                                                                                                      <  2:5146698/139‑1 (MQ=255)
            aTGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTa                                                                                                                               <  1:867557/140‑1 (MQ=255)
            aTGAAGATATTATTCAACCACTTCATCAGCAGTGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGACTTCTTCGGCTGTACGGTCGCTTGATTTTACAATTAATACTGTAGACATTTGTa                                                                                                                               <  1:4095882/140‑1 (MQ=255)
              gAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGTTTTTAAAAAAAATAATGTTTAAATTTTTAtt                                                                                                                             >  1:135480/1‑140 (MQ=255)
                 gATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATtcct                                                                                                                          <  2:2826187/140‑1 (MQ=255)
                  aTCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATtcctc                                                                                                                         <  2:6386480/140‑1 (MQ=255)
                            aCCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTTATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACt                                                                                                               >  2:7590111/1‑140 (MQ=255)
                            aCCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTTCAAAAAATAATGTATTCATTTGTTTTACTCCCGGGAAACt                                                                                                               >  2:3002113/1‑140 (MQ=255)
                            aCCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGAAATTTTTATTACTACCGGTAAACt                                                                                                               >  2:4341708/1‑140 (MQ=255)
                                     tgagcagGGTGATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTTATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATAtt                                                                                                      <  2:4569485/138‑1 (MQ=255)
                                     tcaGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATAtt                                                                                                      <  1:6604724/140‑1 (MQ=255)
                                     tcaGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATAtt                                                                                                      <  1:7066763/140‑1 (MQ=255)
                                     tcaGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATAtt                                                                                                      <  1:6031003/140‑1 (MQ=255)
                                     tcaGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATAtt                                                                                                      <  1:1909183/140‑1 (MQ=255)
                                     tcaGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATAtt                                                                                                      <  2:5062415/140‑1 (MQ=255)
                                       agcagGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTaa                                                                                                    <  1:2850042/140‑1 (MQ=255)
                                         cagGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACt                                                                                                  <  1:4205798/140‑1 (MQ=255)
                                         cagGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATAATGTAGACAGTTGTATTCCTCCCGGTAAACATTTTATATTAACt                                                                                                  >  2:4205798/1‑140 (MQ=255)
                                         cagGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGATTTTAAAAAAAATAATTTAGTCAATTGTGTTACTGCCCGTGAAATTTTTTTTTTAACt                                                                                                  >  1:885103/1‑140 (MQ=255)
                                           gggTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGGTGGATTTTTCAAATAATAATGTAGAGATGTGTTTTGCTCCCGGGTAAGGGTTTATATTGAGTTa                                                                                                >  2:5073647/1‑140 (MQ=255)
                                           gggTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGTTTTTAAAAATAATAAAGTGGAAATTTTTATTACTCCCGGTAAAGATTTTATATTAAATTa                                                                                                >  1:5096409/1‑140 (MQ=255)
                                             gTTATTTTCTTTATAAGCAGCTAAGAAAGCCTAGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACa                                                                                              <  1:1106659/140‑1 (MQ=255)
                                               tATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACaaa                                                                                            <  1:625938/140‑1 (MQ=255)
                                               tATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACaaa                                                                                            <  1:4664483/140‑1 (MQ=255)
                                               tATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACaaa                                                                                            <  2:1410993/140‑1 (MQ=255)
                                                 ttttCTTTATAAGCAGCTAAGAAAGCCTCGTAAATTTTAGTTGAAACGCCTTGTTCGGCTGTACGGTCGCGTGTATTTAAAAATAATAATTTATAAATTTTTTTTACTCCCGGTAAAATTTTTTTATTAACATACACATa                                                                                          >  1:684443/1‑140 (MQ=255)
                                                      tttATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTTCAAATAATAATGTAGAAATTTGTATTACTCCCGGTAAACTTTTTATATTTAATTAAAAATAGTATg                                                                                     >  2:6832522/1‑140 (MQ=255)
                                                      tttATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTAAGGTCGCTTGATTTTAAAAAAAAAAATTTAGACAATTTTATTACTCCCGGTAAAATTTTTATATTAAATAAAAAATAGTAtt                                                                                     >  2:2021314/1‑139 (MQ=255)
                                                             gcagcTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGATTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTActtt                                                                              <  2:3576731/140‑1 (MQ=255)
                                                                gcTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGGTTTTACAAATAAAACTGTAGACATTTGTTTTGCTCCCGGTAAACTTTTTATATTTAAATAAAAAAATTATTTTActttgtt                                                                           >  1:5544736/1‑140 (MQ=255)
                                                                  tAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCtttt                                                                         <  2:1151863/140‑1 (MQ=255)
                                                                   aaGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGATTTTAAAAATAATACTGTTGAACTTTGTATTGGTGCCGGTAAACTTTTTATTTTAACTTAGAAATAGTATGTTTCTTTCttttt                                                                        >  2:7238544/1‑140 (MQ=255)
                                                                         gCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGTTTTTACAAATAATACTGTAGACATTTGTATTCCGCCCGGGAAACTTTTTATATTAACTTACAAATAGGATTTTAGTTTGTTTTTTAAGcc                                                                  >  2:7365795/1‑139 (MQ=255)
                                                                         gCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTAAAAATAATACTGTAGACAATTGTATTACTCCCGGTAAACGTTTTATATTAACTTACAAATAGTATGTTACTTTATTTTTTACGCa                                                                  >  2:2240941/1‑140 (MQ=255)
                                                                           cTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGAACTTTGTATTCCACCCGGTAAACGTTTTATATTAACTTAAAAAAAATATGTTAATTTATTTTTTaatgaaa                                                                >  2:6461637/1‑134 (MQ=255)
                                                                           cTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTAAAAATAATACTGTAGACATTTTTATTCCTCCCGGTAAACTTTTTATATTAAATTACAAATATTATTTTAATTTATTTTTTACGCaaa                                                                >  2:5280314/1‑140 (MQ=255)
                                                                           cTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTGGTTTTTTTAAAAAATAAAGTATACAATTTTTTTGGGGCCGGGCAACTTTTTTTTTTTAATTAAAAATATTATTTTAAtttttttttttagaaaa                                                                >  1:2939709/1‑133 (MQ=255)
                                                                           cTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGTTTTTTAAAAAAAAAAAGTAGTCCATTGTTTTGCGCCCGGTAAACGTTTTATATTAACATAAAAATAGTGTGTGACTTTATTTTTTTAGCaaa                                                                >  1:580018/1‑140 (MQ=255)
                                                                           cTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGTTTTTACAAATAATACTGGAGACCTTTGTTTTGCTCCCGGTAAACGTTTTATATTAACATACAAAAAGTAAGTGTCTTTTTTTTTTTAGCaaa                                                                >  1:639937/1‑140 (MQ=255)
                                                                           cTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGTTTTTAAAAAAAAAAAATTTGACATTTGTTTTACTCCCCGGAAACGTTTTTTATTAAAATAAAAATAGTATTTTACTTTATTTTTTAATCaaa                                                                >  1:6214266/1‑140 (MQ=255)
                                                                           cTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGATTTTACAAATAATACAGTATACATTTGTATTGCTCCCCGTAAACGTTTTATATTAACTTACAAAAATTATGTTAGTTTCTTTTTTTCGCaaa                                                                >  1:5174570/1‑140 (MQ=255)
                                                                              gTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTTATTTTTAAAATAATACAGTATACATATGTAATGCTGCCGGGGAAGTTTTTATATTAACTTACAAATAGTATGTGACTTTATTTTTTACGCaaacta                                                             >  1:7521776/1‑137 (MQ=255)
                                                                              gTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGTTTTTTAAAATAAAAATGTAGACATTTTTATTTCTCCCCGGAAACGTTTTATATTAAATTACAAAAATTATGTTACTTTCTTTTTTAAGCAAAGTc                                                             >  1:1537382/1‑140 (MQ=255)
                                                                                  aGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATc                                                         <  1:396328/140‑1 (MQ=255)
                                                                                  aGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATc                                                         <  1:1061202/140‑1 (MQ=255)
                                                                                  aGTTTAGTTGAAACGCCTTCGTCGGCTGTACGGTCGCTTGAGTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAACTAGTATGTTACTTTCTTTTTTACGCAAAGTCAATc                                                         <  1:2527576/140‑1 (MQ=255)
                                                                                    tttAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCGTGTTTTTACAAATAATAATGTATTCATTTTTATTGCACCCCGGCAACGTTTTTTATTAAATTAAAAAAAGTATTTTTATTTATTTTTTTAGCAAACTCAAacac                                                       >  1:359511/1‑140 (MQ=255)
                                                                                        gTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATTATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTAATTTATTTTTTGAGCAAAGTGAATCACTTTa                                                   >  2:4299485/1‑140 (MQ=255)
                                                                                         ttGAAACGCCTTCTTCGGCTGTACGGTCGCTTGGTTTTTAAAATAAAACTTTAGACATTTTTATTACTCCCGGGAAACATTTTTTATTTACTTACAAAAAGTATGTTTATTTGTTTTTTAAGCAAACTCAATCAAttttt                                                  >  1:5781399/1‑138 (MQ=255)
                                                                                              aCGCCTTCTTCGGCTGTACGGTCGCTTGGTTTTAAAAATAATACAGTAGACAATTGTATTACTCCCCGTAAAATTTTTATATTAAATTAAAAATATTATTTTACTTTATTTTTTTAGCAAAATGAATAAATTTATTTTGa                                             >  2:708173/1‑140 (MQ=255)
                                                                                              aCGCCTTCTTCGGCTGTACGGTCGCTTGATTTTAAAAATAAAAAAGTTGACAATTGTATTCCGCCCGGGAAACATTTTATATTAACTTACAAATAGTATGTTAATTTATTTTTTAAGCAAAGTCAAACAAGTTATTTTgt                                             >  2:7061941/1‑139 (MQ=255)
                                                                                                                        tGATTTTTCAAAAAATAATTTAGTCAATTGTATTCCTCCCCGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTGTTTTTTAATCAAAGTCAATCACATTATTTTTAATATTAGATTTTTTTTAAAAAAAAAg                   >  2:7129306/1‑140 (MQ=255)
                                                                                                                        tGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGTTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAACCACTTTATTTTGAATATGACATTTTTTTTCAAAAAAAAg                   <  2:684443/140‑1 (MQ=255)
                                                                                                                        tGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAg                   <  2:177905/140‑1 (MQ=255)
                                                                                                                        tGAATTTAAAAAAAAAACAGTATACAATTTTATTGCTCCACGTAAACTTTTTATATTAACTTACAAATACTATGTTAATTTAATTTTTAAGCAAAATAAAAAAAGTTATTTTGAATATGAGAATTATTTTCaaaaaaaaa                   >  2:4483852/1‑139 (MQ=255)
                                                                                                                            tttACAAATAATACTGTAGACCTTTGTATTCCTCACGGTAAACTTTTTATATTCACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAg               <  2:1276589/140‑1 (MQ=255)
                                                                                                                            cttACAAATAATCCGGGAGACATTGGTATTCCTCCCGGTAAACTTTTTATCTTACCTTACAACTAGAATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTATGAATATGAGATTTATTTTCAAAAAAAAGCAAg               <  2:5665399/139‑1 (MQ=255)
                                                                                                                             ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt              <  2:3958823/140‑1 (MQ=255)
                                                                                                                             ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt              <  2:891818/140‑1 (MQ=255)
                                                                                                                             ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt              <  2:848276/140‑1 (MQ=255)
                                                                                                                             ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt              <  1:240533/140‑1 (MQ=255)
                                                                                                                             ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt              <  2:5910441/140‑1 (MQ=255)
                                                                                                                             ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt              <  2:2170830/140‑1 (MQ=255)
                                                                                                                             ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt              <  2:5781399/140‑1 (MQ=255)
                                                                                                                             ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt              <  2:4091542/140‑1 (MQ=255)
                                                                                                                             ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt              >  2:2531696/1‑140 (MQ=255)
                                                                                                                             ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt              <  2:3654514/140‑1 (MQ=255)
                                                                                                                             ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt              <  2:2977724/140‑1 (MQ=255)
                                                                                                                             ttACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACGTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt              <  2:2972795/140‑1 (MQ=255)
                                                                                                                             ttACAAATAATAATGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt              <  2:4356196/140‑1 (MQ=255)
                                                                                                                             ttACAAATAATAAGGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt              <  2:5544736/140‑1 (MQ=255)
                                                                                                                             atACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTGACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGt              <  2:6517447/139‑1 (MQ=255)
                                                                                                                                 aaaTAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACAt          <  2:167633/140‑1 (MQ=255)
                                                                                                                                 aaaTAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACAt          <  2:135480/140‑1 (MQ=255)
                                                                                                                                 aaaTAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACAt          <  2:3502482/140‑1 (MQ=255)
                                                                                                                                 aaaTAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACAt          <  2:6042162/140‑1 (MQ=255)
                                                                                                                                     tataATGTAGATATTTGTATTACTCCCGGTTAACTTTTTTTATTAACTTACATATAGTATTTTACTTTCTTTTTTTCGCAAAGTCAATCACTTTATTTTGAATATGAGAGTTTTTTTCAAAAAAAAGCAAGTACATCTat      <  1:4539259/139‑2 (MQ=255)
                                                                                                                                     aataCTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTaa      <  1:3411839/140‑1 (MQ=255)
                                                                                                                                     aataCTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTaa      <  2:2485631/140‑1 (MQ=255)
                                                                                                                                        aCTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCTATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAatat   <  2:2939709/140‑1 (MQ=255)
                                                                                                                                        aCTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAatat   <  2:6214266/140‑1 (MQ=255)
                                                                                                                                        aCTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAatat   <  1:6832522/140‑1 (MQ=255)
                                                                                                                                        aCTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAatat   <  1:4475802/140‑1 (MQ=255)
                                                                                                                                        aCTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAatat   <  1:4037059/140‑1 (MQ=255)
                                                                                                                                        aCTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAatat   <  1:3160734/140‑1 (MQ=255)
                                                                                                                                        aCTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAatat   <  1:2021314/140‑1 (MQ=255)
                                                                                                                                        aCTGTAGACATTTGTAGTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAatat   <  1:676086/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
AAGGTTCTCTTTATGAAGATCTAATTCAACCACTTCATCAGCAGGGTTATTTTCTTTATAAGCAGCTAAGAAAGCCTCGTAAAGTTTAGTTGAAACGCCTTCTTCGGCTGTACGGTCGCTTGATTTTACAAATAATACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTAACTTACAAATAGTATGTTACTTTCTTTTTTACGCAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAGCAAGTACATCTAATATC  >  NZ_CP034943/2103637‑2103913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: