Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,139,480 A→C 8.4% A119A (GCT→GCG deoD ← purine‑nucleoside phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,139,4800AC 8.4% 1358.1 / 22.5 525A119A (GCT→GCGdeoDpurine‑nucleoside phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (335/122);  new base C (2/66);  total (337/188)
Fisher's exact test for biased strand distribution p-value = 7.12e-31
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 530 total aligned reads displayed.

CGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAATTCATTCA  >  NZ_CP034943/2139345‑2139619
                                                                                                                                       |                                                                                                                                           
cGAATCGTGTTTGTAGAACTGATCAGCTTTAAATACGCTTCCTCCCGTCACCGGCAGGCGTTTATCATTTGCGGCATCATAGGAATTTTTTAAAAGCTCGAAATCGGCGCAAGGCGCAAAATCAATACTTCCCAACGCGa                                                                                                                                         <  2:3020207/140‑1 (MQ=255)
  aaTCGTCGTTGTAGAACTGATCAGATTTAAATACGCTTCATACCGTCACCGGCAGGATTTTATCATTTGCGCCATAATTGGCATTTTTTAAAAGCTCTAAATCTGCGCAAGGCTCAAAATCATTACTTCCGAAAGCGAct                                                                                                                                       <  2:3997570/140‑1 (MQ=255)
             tAGAACTGATCCGCTGTAAATACGCTTCCTACCGTCACCGGCACGCATTTCTCGTTTGCGGAATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGAAAAATCAATACTTCCGAAAGCGACTCTGTTCATTtg                                                                                                                            <  2:6429901/140‑1 (MQ=255)
             tAGAACTGATCAGCTGTAAATACGCTTCCCCCCGTCACCGGCACGCCTTTAGCCTTTGCGGCATCATAGGCATTTTTTAAACGCTCGAAATCGGCGCAAGGCGCAAAATCAATCCTCCCGAAAGCGACTCTGTTCATTtg                                                                                                                            <  2:221548/140‑1 (MQ=255)
                aaCTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGa                                                                                                                         >  1:6486929/1‑140 (MQ=255)
                 agtgctCAGCTGTACATCCGGTTCCTACGGTCACGGGCACGCCTTTTTCCTTGGCGGCATAATAGGCATTTTTTAAACGAGCGAAATGGGCGCACGGCCAAAATTCATTCCTTCCCAAAGCGACTCTGTTCATTTGTGaa                                                                                                                        <  2:1480780/135‑1 (MQ=255)
                     aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGGCTGTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGTATcgg                                                                                                                    >  1:5805944/1‑138 (MQ=255)
                     aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  1:6233835/1‑140 (MQ=255)
                     aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  1:5054744/1‑140 (MQ=255)
                     aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  1:4693549/1‑140 (MQ=255)
                     aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  1:447280/1‑140 (MQ=255)
                     aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  1:3737400/1‑140 (MQ=255)
                     aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  2:4496719/1‑140 (MQ=255)
                     aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  1:2995096/1‑140 (MQ=255)
                     aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  1:2325711/1‑140 (MQ=255)
                     aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  1:2179685/1‑140 (MQ=255)
                     aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATGTGTGAATCAg                                                                                                                    >  1:1400355/1‑140 (MQ=255)
                     aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTAGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  2:6368995/1‑140 (MQ=255)
                     aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGACTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCTAGGCGCAATATCAATAGTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  1:2621472/1‑140 (MQ=255)
                          cTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAg                                                                                                               >  1:3129767/1‑140 (MQ=255)
                          cTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAg                                                                                                               >  1:3360632/1‑140 (MQ=255)
                          cTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAg                                                                                                               >  1:4315222/1‑140 (MQ=255)
                               aaTACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGt                                                                                                          >  2:3773418/1‑140 (MQ=255)
                                 tACGCTTCCTACCGTCACCGGCCCGCCTTTTTCTTTGGCGGACACAAAGCCTTTTTTTAAAAGCTAGAAATCTGCGAACGGCCAAAAATCAATACTTCCCACCGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCa                                                                                                        <  1:6834376/140‑1 (MQ=255)
                                 cccGCTCCCAACCGACACCGGCACGCCTTTAACCTTTGCGGCATCCTCGGCTTTTTTTAAAAGCTCGAAATCGGGGCAAGGCCCAAAATCAAACCGTCCCAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCa                                                                                                        <  1:63873/138‑1 (MQ=255)
                                    gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                                     >  1:2719741/1‑140 (MQ=255)
                                            ccGTCACCGCCCCGCGTTTCCCCTTTGCGGAATAATAGGCATTTTTTAAAAGCTCGAAATCGGCGAAAGGCGAAAAATCAATACTACCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATg                                                                                             <  2:1947761/140‑1 (MQ=255)
                                                cACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGt                                                                                         >  2:5923937/1‑140 (MQ=255)
                                                cACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGt                                                                                         >  2:2472362/1‑140 (MQ=255)
                                                cACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGt                                                                                         >  1:2855341/1‑140 (MQ=255)
                                                  ccGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTcc                                                                                       >  1:199057/1‑140 (MQ=255)
                                                  ccGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTcc                                                                                       >  1:7634138/1‑140 (MQ=255)
                                                  ccGGCACGCCTTTAGCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTCCCAAAGGCGCAAAATCAATACTTCCTAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTcc                                                                                       <  2:6174505/140‑1 (MQ=255)
                                                    ggCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTACCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCg                                                                                     <  2:7054661/140‑1 (MQ=255)
                                                     gcccGCCTTTATCCTTTGCGCCACCATCGCCTTTTTTGAACCGCCCGAAAACTCCCCACGGCGCAAAATCAATCCGCCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGc                                                                                    <  1:6942560/137‑1 (MQ=255)
                                                           cTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTg                                                                              >  2:1713231/1‑140 (MQ=255)
                                                              tATCATTTGCGGCATCATAGGCATTTTTTAAAATCTCGAAATCTGCGAACGGCGAAAAATCAATAGTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACa                                                                           <  1:3641245/140‑1 (MQ=255)
                                                                    ttGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTg                                                                     >  1:7163432/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGACAGCGACTCTGTGCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:7512903/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGa                                                                                                                         <  2:6105537/87‑1 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAgg                                                                                                              >  1:4789330/1‑98 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGAc                                                                            >  1:5737383/1‑132 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGAGATCTTTGc                                                                    >  1:1559466/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTga                                                                    >  1:1080471/1‑139 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:6114227/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  2:7513177/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:2110424/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:242565/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:2478517/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:3282276/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  2:428802/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:3472209/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  2:4658534/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:923204/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:848101/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  2:4784953/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  2:6862133/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:4600244/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:7389194/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  2:4965590/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:5150088/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:5201083/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:5281332/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  2:5221748/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:6573779/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  2:5710619/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:5573910/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  2:5460905/1‑140 (MQ=255)
                                                                     tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTGTGc                                                                    >  1:5922220/1‑140 (MQ=255)
                                                                          catcatAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATa                                                               >  1:1907029/1‑140 (MQ=255)
                                                                             catAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTTCGGATAgcg                                                            >  2:7657873/1‑140 (MQ=255)
                                                                             catAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAgcg                                                            >  2:820673/1‑140 (MQ=255)
                                                                             catAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAgcg                                                            >  2:2085250/1‑140 (MQ=255)
                                                                               tAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGcgcc                                                          >  1:4196109/1‑140 (MQ=255)
                                                                                aGGCATTTTTAAAAAGCTCTAAATCTGCGCAAGGCGAAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGcgccg                                                         <  1:1647850/140‑1 (MQ=255)
                                                                                  tcATTTTTTAAAAGCTCGAAATATGCGCAAGGCGCAAAATCAATACGTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                       <  2:6651604/139‑1 (MQ=255)
                                                                                  gCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                       <  2:6745340/140‑1 (MQ=255)
                                                                                      tttttAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGac                                                   >  1:3996073/1‑139 (MQ=255)
                                                                                       ttttAAAAGCTCTAAATCTGCGCAAGGCGAAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAAc                                                  <  1:3969069/140‑1 (MQ=255)
                                                                                       ttttAAAAGCTCGAACTCTGCGCACGGCGAAAAATCAATCCTTCCTAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAAc                                                  <  2:2854653/140‑1 (MQ=255)
                                                                                          tAAAAGCTCTAAATCTGCGCAAGGCGAAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTa                                               <  2:7597935/140‑1 (MQ=255)
                                                                                           aaaaGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTGCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTAc                                              <  1:7687613/140‑1 (MQ=255)
                                                                                            aacgCTCGAAATGTGCGCACGGCGAAAAAAAAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTAct                                             <  1:4973797/137‑1 (MQ=255)
                                                                                            aaaGCTCGAAATCTGCGCAGGGCGCAAAATCAATCCTTCCGAAAGCGACTCTCTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTAct                                             <  1:5429100/140‑1 (MQ=255)
                                                                                                cTCGAAATCTGCGCAAGGCGCAAAATAAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCtta                                         <  1:1713231/140‑1 (MQ=255)
                                                                                                  cGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCttatt                                       >  2:129288/1‑140 (MQ=255)
                                                                                                  cGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGAGGATAGCGCCGCAAGAACCTACTCttatt                                       >  2:7201742/1‑140 (MQ=255)
                                                                                                  cGAAATCTCCGCAAGCCGCAAAAACAATACTTCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCttatt                                       <  1:6036129/140‑1 (MQ=255)
                                                                                                       tCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGAtt                                  <  2:3365979/140‑1 (MQ=255)
                                                                                                          gcgcATGGCGCAAAATACATACTTCGTAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTg                               <  2:1094888/140‑1 (MQ=255)
                                                                                                           cgcAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTTCGGATAGCGCCGCAAGAACCTACTCTTCTTATAtttttc                              >  1:3809637/1‑138 (MQ=255)
                                                                                                           cgcAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGc                              >  1:6728052/1‑140 (MQ=255)
                                                                                                            gcACGGCGAAAAATCAATACTGCCCCACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCACAATGACGTCCCGCACTTTGACATCATTGCGGATAGCGCCGCAAGAACCTACTCTTCTTAGATTTTGca                             <  1:7231524/140‑1 (MQ=255)
                                                                                                            gcAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGca                             <  2:5054744/140‑1 (MQ=255)
                                                                                                                 gcgcAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCg                        >  2:1012237/1‑140 (MQ=255)
                                                                                                                  cgcAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGt                       >  1:4903913/1‑140 (MQ=255)
                                                                                                                      aaaCCAATACGCCCCAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCAGAGCCAAAATGACGTCCCGCACATTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCt                   <  2:4400510/140‑1 (MQ=255)
                                                                                                                           aaTACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGaa              <  1:1289575/140‑1 (MQ=255)
                                                                                                                            aTACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGaat             >  1:1021208/1‑140 (MQ=255)
                                                                                                                                       aGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACTTCACGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAAttcattca  >  2:3250563/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                           
CGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAATTCATTCA  >  NZ_CP034943/2139345‑2139619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: