BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,214,528	G→T	6.2%	Q57K (CAA→AAA)	nth ←	endonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,214,528	0	G	T	6.2%	1139.8 / 13.5	461	Q57K (CAA→AAA)	nth	endonuclease III
Reads supporting (aligned to +/- strand): ref base G (80/333); new base T (36/8); total (116/345)
Fisher's exact test for biased strand distribution p-value = 1.16e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 465 total aligned reads displayed.

CCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGA  >  NZ_CP034943/2214389‑2214667

   ccgTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTaaa                                                                                                                                          >  1:6235232/1‑140 (MQ=255)

   ccgTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGaaa                                                                                                                                          >  2:4550602/1‑140 (MQ=255)

     gTAATCTTCAATCATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAaata                                                                                                                                        <  2:7477869/140‑1 (MQ=255)

     gTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAaata                                                                                                                                        <  2:4082503/140‑1 (MQ=255)

      tAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTTaaaaaa                                                                                                                                       >  1:4064208/1‑140 (MQ=255)

      tAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCGTCCGGCCGTTTATTTTTTTGAaataa                                                                                                                                       >  1:5485029/1‑140 (MQ=255)

         tCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGt                                                                                                                                    <  2:6606039/140‑1 (MQ=255)

         tCTTCAATAATCATTGTGCTCAGTTTTTTGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGt                                                                                                                                    <  2:3644564/140‑1 (MQ=255)

           ttCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTTAAGCTCTTCCAGCGAAACAGCCAAATATTCTTCCGGCCGTTTATTTTTTTGAAATAATGtgt                                                                                                                                  >  2:6755007/1‑138 (MQ=255)

           ttCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGttt                                                                                                                                  <  2:3241697/140‑1 (MQ=255)

              aataatCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTTAATAATTGTTTgg                                                                                                                               >  1:6347302/1‑140 (MQ=255)

              aataatCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTgg                                                                                                                               >  2:6264186/1‑140 (MQ=255)

                taatCATTTTTCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTc                                                                                                                             <  2:4992590/140‑1 (MQ=255)

                 aatCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCa                                                                                                                            <  1:1175394/140‑1 (MQ=255)

                 aatCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTGGTCa                                                                                                                            <  1:1670021/140‑1 (MQ=255)

                  atCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAc                                                                                                                           <  1:2040245/140‑1 (MQ=255)

                  atCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAc                                                                                                                           <  1:2723753/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  2:5712906/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  1:780760/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  2:5900825/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  1:6471891/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  2:1367373/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  2:6099052/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  2:7680191/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  1:3933888/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  2:4805240/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  2:2226144/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCAAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                          <  2:173015/140‑1 (MQ=255)

                   tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACAGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAACGTTTTGGTCAct                                                                                                                          <  1:47704/140‑1 (MQ=255)

                   tCATGTTGCTCTGTTTGTGGTTGTTTGTCTCTTTATTACGATACATACCGATTGATTTAAGAGCCTGCTGAAGCTCTTCCTGCGATCCAGGGAAATAGTCTTCCGGCCGTGTATAGTTTTCAAATAATGTTTTGGTCAct                                                                                                                          <  2:6019896/140‑1 (MQ=255)

                              aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCTATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGACAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  2:3092596/140‑1 (MQ=255)

                              aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  1:4890730/140‑1 (MQ=255)

                              aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  1:7640442/140‑1 (MQ=255)

                              aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  2:1466025/140‑1 (MQ=255)

                              aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  1:4503326/140‑1 (MQ=255)

                              aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  2:4192817/140‑1 (MQ=255)

                              aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  2:1995560/140‑1 (MQ=255)

                              aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTGGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                               <  2:5447725/140‑1 (MQ=255)

                                tttttGGCTGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAAGATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  2:5205782/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  1:1022503/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  2:2061516/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  2:297905/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  2:3165694/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  1:4180729/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  2:3798546/140‑1 (MQ=255)

                                tttttGGATGTTTTTCGCTTTAGTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  2:7588711/140‑1 (MQ=255)

                                ttgttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             <  1:3417162/137‑1 (MQ=255)

                                   ttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCAt                                                                                                          <  2:44610/140‑1 (MQ=255)

                                    tGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCGGTTTACAAGTGCATc                                                                                                         <  2:7007003/140‑1 (MQ=255)

                                     ggATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCActct                                                                                                                        <  1:7072034/124‑1 (MQ=255)

                                          tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATAATTTTGAAATAATGTTTTTGTGACACTGTTTAAAAGTGCATCTGTACa                                                                                                   >  2:1014409/1‑140 (MQ=255)

                                          tttttCGCTTTATTAAGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTGATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACa                                                                                                   <  2:1946788/140‑1 (MQ=255)

                                               cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                              <  1:3142042/140‑1 (MQ=255)

                                               cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTGGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                              <  2:161103/140‑1 (MQ=255)

                                                       taCTATACAAACCGATTGATTTAATATCCTGCTTAAGCTCTTCCAGCGAAACAGCAAAATCTTCTTCCGGCGGTTTATATTTTTTAATTAATTTTTTGGTCACTCTGTTTACAAGTGAATCTTTACATTGCGCAGATaaa                                                                                      <  1:7412851/140‑1 (MQ=255)

                                                          gATACAAACCGATTGATTTAATATCATGCTGAAGCTCTTCCAGCGACACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCg                                                                                   <  2:3376958/140‑1 (MQ=255)

                                                           aTACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTGTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGa                                                                                  <  1:2319881/140‑1 (MQ=255)

                                                            tACAAACCGATTGATTTAATATCCTGCTGAATCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTAAATTTTTGAAATTATGTTTTTGTCACTAGGTTTACAAGTTCATCAGGAAAGTGCGCAGATAAAGCgtc                                                                                 >  2:1185138/1‑138 (MQ=255)

                                                                aaCCGTTTGATTTAATATCCTGCAGAAGCTCTTCCAGCTAAACAGCCAAATAGTCTTCCGGCCGTTTTTATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTAAATTGCGCAGATAAAGCGACTGCt                                                                             <  1:2957966/140‑1 (MQ=255)

                                                                aaCCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCt                                                                             <  2:68434/140‑1 (MQ=255)

                                                                      ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                             >  2:6464854/1‑102 (MQ=255)

                                                                      ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTTTAATAATTTTTTTGTGACTCGGTTTAAAAATGCATCTGTACAATGCGCACATAAAGCGACTGCTACAAca                                                                       >  1:2132902/1‑139 (MQ=255)

                                                                      ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCCGCCGTTTATATTTTTGAAAAAAAGTTTTTGTAAATATTTTTACAAATGCAACTGTAAAATGCGAAAATAAACCGACTGATAacaca                                                                       >  2:4337487/1‑139 (MQ=255)

                                                                             aaTATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCaa                                                                <  1:4728284/140‑1 (MQ=255)

                                                                                   ctgctgACGCTATTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTCAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                          <  1:4571099/140‑1 (MQ=255)

                                                                                   ctgctgAAGCTCTTCCCGCGAAACAGCCAAATTGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                          <  1:1468301/140‑1 (MQ=255)

                                                                                   ctgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTGTATTTTTGTAATAATGTTTTGGGGACTCTGGTTTAAAGTGCATGTGTACATTGGGCCGATAAAGCGGCTGCTACCACAAAATGAAAATGAt                                                          >  1:6413038/1‑140 (MQ=255)

                                                                                    tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTATTTTTGAAATAATGTTTTTGTCACTCTGTTTACAACTGCATCGGTACATTGCGCAGATAAAGCGACTGCGAACACTAATTCAAAAGGAtt                                                         >  1:5717659/1‑140 (MQ=255)

                                                                                    tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGGTTTTATTTTTGAAATAATGTTTTTGTGACTCGGTTTAAAAGTGCATGTGTAAAATGGGCAGATAAAGCGACTGATACCACTAATTCAAAACGAtt                                                         >  1:5363874/1‑140 (MQ=255)

                                                                                        gAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGaa                                                     <  2:186707/140‑1 (MQ=255)

                                                                                          aGCTCTTCCAGCGAAACAGCCACATAGTCTTCCGGCCGTTTTCATTTTTGAAATAATGTTTTGGGCACTCTGTTTACAAGTGCATATGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATg                                                   <  1:2509574/140‑1 (MQ=255)

                                                                                           gCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAACTCATGTTTTTGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGCGTGCTCCCACTAATTCAAAAGGAGTGGAATGa                                                  >  2:472973/1‑140 (MQ=255)

                                                                                            ctgttCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTGGAAATAATGTTTTGGTCACTCTGTTTAAAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                 <  1:3777310/137‑1 (MQ=255)

                                                                                            ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCCGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                 <  1:5460135/140‑1 (MQ=255)

                                                                                            ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGAATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                 <  1:4255936/140‑1 (MQ=255)

                                                                                            ctctTCCAGCGAAACAGCCAAATAGTCGTCCGGCCGTTGATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                 <  2:5727212/140‑1 (MQ=255)

                                                                                            ctctTCCAGCGAAACAGCAAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATTTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                 <  1:3716529/140‑1 (MQ=255)

                                                                                            ctctTCCAGAGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTGTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                 <  2:7117619/140‑1 (MQ=255)

                                                                                              ctTCCCGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                               <  1:7681818/140‑1 (MQ=255)

                                                                                              ctTCCAGCTAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACTTTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                               <  1:3070743/140‑1 (MQ=255)

                                                                                                  cAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGtt                                           <  2:725263/140‑1 (MQ=255)

                                                                                                           aGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTg                                  <  2:4881139/140‑1 (MQ=255)

                                                                                                           aGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTg                                  <  2:3730389/140‑1 (MQ=255)

                                                                                                           aGCCAAATAGTCTTCCGGCCGTTTATAGTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTg                                  <  2:4031389/140‑1 (MQ=255)

                                                                                                               aaaTAGTCTTCCGGCCGTTTATATTTGTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCgtg                              <  1:665286/140‑1 (MQ=255)

                                                                                                                 aTAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGtgtg                            <  2:2181796/140‑1 (MQ=255)

                                                                                                                 aTAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGtgtg                            <  2:5581059/140‑1 (MQ=255)

                                                                                                                       ttCCGGCCGTTTATATTTTTTAAATAATTTTTTGGTCACTCTGTTTACAAGTGCATATGTACATTGCGCAGATAAAGCGACAGCTACCACTAATTCAAAAGGATTGGAAAGAAACACTTCACACTCTGCGTGTGGAAACa                      >  2:3905924/1‑140 (MQ=255)

                                                                                                                        tCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACAt                     <  1:6091799/140‑1 (MQ=255)

                                                                                                                          cGGCCGTTTATATTTTTGTAATAATGTTTTGGTCACTCTGTTTACAACTGCAACAGTACAAGGCGCAGATAAATCGACTTCTACCACTAATTAAAAAGGATTGGAAAGAACCACTTCACACACTGCGTGTGGCAACAAGt                   >  2:2430533/1‑140 (MQ=255)

                                                                                                                            gCCGTTTATATTTTTGAAAAAATGTTTTGGTAACTCTGTTTACAATTGCATCAGTACATTGCGCAGATAAAACGACTGCTCCCACAAATTCAAAAGGATTGGAATGAAACATGTCACACAATGCGTGTGGAAACATGTCg                 >  2:799821/1‑140 (MQ=255)

                                                                                                                                 ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt            <  1:5236877/140‑1 (MQ=255)

                                                                                                                                 ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt            <  1:5062025/140‑1 (MQ=255)

                                                                                                                                 ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt            <  1:6858868/140‑1 (MQ=255)

                                                                                                                                 ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt            <  2:2867703/140‑1 (MQ=255)

                                                                                                                                 ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt            <  1:1946082/140‑1 (MQ=255)

                                                                                                                                 gtataTTTTTGAAATAATGTTTTGGTCTCTGTGTTTAAAAGTGCATCTGTACATTGCGCCGATAAAGCGACTGCTACCAGTACTTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt            <  1:903060/139‑1 (MQ=255)

                                                                                                                                   atatTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACAGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCt          <  2:2105121/140‑1 (MQ=255)

                                                                                                                                   atatTTTTGAAATAATGTTTTGGACACTCTGTTTAAAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCt          <  2:5108674/140‑1 (MQ=255)

                                                                                                                                        tttGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCt     <  2:6347302/140‑1 (MQ=255)

CCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGA  >  NZ_CP034943/2214389‑2214667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`