Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,214,541 G→T 6.7% T52T (ACC→ACA nth ← endonuclease III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,214,5410GT 6.7% 1205.8 / 16.9 423T52T (ACC→ACAnthendonuclease III
Reads supporting (aligned to +/- strand):  ref base G (49/330);  new base T (38/4);  total (88/335)
Fisher's exact test for biased strand distribution p-value = 2.41e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 430 total aligned reads displayed.

AATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAAT  >  NZ_CP034943/2214403‑2214678
                                                                                                                                           |                                                                                                                                         
  taatCATTTTTCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTc                                                                                                                                        <  2:4992590/140‑1 (MQ=255)
  taatCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAATAAATTTTTTGTc                                                                                                                                        >  1:5590908/1‑140 (MQ=255)
   aatCATTTTGCTCAGTTTTTGGATGATTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCGTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCa                                                                                                                                       <  1:5819790/140‑1 (MQ=255)
     tCATTTTGCTCATTTTTTGGATGTTTTTCGCTTTATTACGATACCAACCGATTGATTTAATAGCCTGATGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATTTTTTTGAAATAATGTTTTGGTCAct                                                                                                                                     <  1:6446291/140‑1 (MQ=255)
     tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAATCCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                                     <  2:1910830/140‑1 (MQ=255)
     tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                                     <  1:4604929/140‑1 (MQ=255)
     tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                                     <  1:780760/140‑1 (MQ=255)
     tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                                     <  1:7446914/140‑1 (MQ=255)
     tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                                     <  1:6537332/140‑1 (MQ=255)
     tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                                     <  1:6375419/140‑1 (MQ=255)
     tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                                     <  2:2226144/140‑1 (MQ=255)
     tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                                     <  2:4732975/140‑1 (MQ=255)
     tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                                     <  1:2201339/140‑1 (MQ=255)
     tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                                     <  1:3048211/140‑1 (MQ=255)
     tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                                     <  1:2898523/140‑1 (MQ=255)
     tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                                     <  2:7387550/140‑1 (MQ=255)
     tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                                     <  2:5712906/140‑1 (MQ=255)
     tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                                     <  2:7543554/140‑1 (MQ=255)
                aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCTATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGACAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                          <  2:3092596/140‑1 (MQ=255)
                aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCCGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                          <  1:5245989/140‑1 (MQ=255)
                aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                          <  2:1373653/140‑1 (MQ=255)
                aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                          <  2:6053278/140‑1 (MQ=255)
                aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                          <  2:1466025/140‑1 (MQ=255)
                aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                          <  1:4405585/140‑1 (MQ=255)
                aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                          <  2:3685942/140‑1 (MQ=255)
                aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTGGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                          <  2:5447725/140‑1 (MQ=255)
                  tttttGGCTGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAAGATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                        <  2:5205782/140‑1 (MQ=255)
                  tttttGGATGTTTTTCGCTTTATTACTATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                        <  2:2992457/140‑1 (MQ=255)
                  tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                        <  1:3217149/140‑1 (MQ=255)
                  tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                        <  1:1402490/140‑1 (MQ=255)
                  tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                        <  1:91236/140‑1 (MQ=255)
                  tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                        <  2:2065844/140‑1 (MQ=255)
                  tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                        <  2:2386290/140‑1 (MQ=255)
                  tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                        <  2:3165694/140‑1 (MQ=255)
                  tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                        <  2:3207834/140‑1 (MQ=255)
                  tttttGGATGTATTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                        <  2:1783144/140‑1 (MQ=255)
                     ttGGATGTTTTTCGCTTTATTACTATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCAt                                                                                                                     <  2:7672584/140‑1 (MQ=255)
                       ggATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCActct                                                                                                                                   >  2:4041140/1‑124 (MQ=255)
                            tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATAATTTTGAAATAATGTTTTTGTGACACTGTTTAAAAGTGCATCTGTACa                                                                                                              >  2:1014409/1‑140 (MQ=255)
                            tttttCGCTTTATTAAGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTGATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACa                                                                                                              <  2:1946788/140‑1 (MQ=255)
                                 cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                                         <  1:2399832/140‑1 (MQ=255)
                                 cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                                         <  2:5775930/140‑1 (MQ=255)
                                 cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTGGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                                         <  2:161103/140‑1 (MQ=255)
                                 cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTGATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                                         <  2:788069/140‑1 (MQ=255)
                                 cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTATTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                                         <  2:6199208/140‑1 (MQ=255)
                                 cGCTTTATTACGAAACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGATTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                                         <  2:4490348/140‑1 (MQ=255)
                                      tattaCGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGTAATAAAGTTTTTGTCACTCTGTTTACAAGTGGAAATGTCCATTGCGCAGAt                                                                                                    >  1:7649934/1‑140 (MQ=255)
                                         taCTATACAACCCGATTTATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAATAATTTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATaaa                                                                                                 <  1:3093086/140‑1 (MQ=255)
                                             aTACAAACCGATTGATTTAATATCCTGCTTAAGATCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGa                                                                                             <  2:6914999/140‑1 (MQ=255)
                                              tACAAACCGATTGATTTAATATCCTGCTGAATCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTAAATTTTTGAAATTATGTTTTTGTCACTAGGTTTACAAGTTCATCAGGAAAGTGCGCAGATAAAGCgtc                                                                                            >  2:1185138/1‑138 (MQ=255)
                                                   aCCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                                     <  1:6874083/94‑1 (MQ=255)
                                                   aCCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATTTTTTTGTGACTCTGTTTTAAAGTGCATCGGTAAAGTGGGCAGATAAAGCGACAGcga                                                                                       >  1:2105121/1‑138 (MQ=255)
                                                       attgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTTGTAAATCTGTTTACAAGTTCATATTTAAATTGCGCAGATAAAACGACTGCTACCAc                                                                                   >  2:7061582/1‑140 (MQ=255)
                                                       attgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                        <  1:7707405/103‑1 (MQ=255)
                                                       attgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGTAATAAATGTTTGGTGACGCTGTTTACAATTGCATCTGTACCATGCGCAGATAAATCCACTGCTACCAc                                                                                   >  1:6269104/1‑140 (MQ=255)
                                                                     ctgcggATGGTCTTCCCGCTAAACCGCAAAATAGTATTCCGGCCGTTTTTATTTTTGAAATATTGTTTTTGTCACTCTGTTTAAATGTGCATGTGTACTTTGCGCCGCTAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                                     <  1:194156/140‑1 (MQ=255)
                                                                      tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAtt                                                                    >  2:4187957/1‑140 (MQ=255)
                                                                      tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTATTTTTTTAAAAAATGTTTTTTGACACGGTTTACAATTGGATCTGTCAAATTAGCACATAAAGACACTGCAACCACGAATTAAAAAgagtt                                                                    >  1:725263/1‑136 (MQ=255)
                                                                      tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTATTTTTTAAATAATTTTTTTGTCCCTCTGTTTACAAGTGCATCTGTACATTTCGCAGATAAAGCGACTGCTACCACAAAATCAAAAGGAtt                                                                    >  1:5445427/1‑140 (MQ=255)
                                                                      tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTTTAATAATTTTTTTGTGACTCTGTTTAAAAGTGCATCTGTAAATTTCGCAGATAAAGCGACATCTACCACAAATTAAAAAGGCtt                                                                    >  1:3940174/1‑140 (MQ=255)
                                                                      tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGGTTTTATTTTTGAAATAATGTTTTTGTGACTCGGTTTAAAAGTGCATGTGTAAAATGGGCAGATAAAGCGACTGATACCACTAATTCAAAACGAtt                                                                    >  1:5363874/1‑140 (MQ=255)
                                                                      tgctcAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAAAAAAGGTTTTGTGACTGAGTTTTAAACCGCATCAGTAAAATTCGCACATAAATAGACAGCTACCACAAATTCaaaaacata                                                                    >  1:3838934/1‑135 (MQ=255)
                                                                        ctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAATAATGTTTTTGTCACTCTGTTTACAAGTGCAACTGTACATTGCGCAGATAAAGCGACTGCTACCACTAAATCAAAAGGATTgg                                                                  >  2:6416659/1‑140 (MQ=255)
                                                                        ctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                                     <  1:672428/137‑1 (MQ=255)
                                                                           aaGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGt                                                                                                                         <  1:4968489/82‑1 (MQ=255)
                                                                              ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                            <  1:3544577/140‑1 (MQ=255)
                                                                              ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                            <  1:410281/140‑1 (MQ=255)
                                                                              ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                            <  2:7181242/140‑1 (MQ=255)
                                                                              ctctTCCAGCGAAACAGCAAAATAGTCTTCCGGCCTTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                            <  1:4550602/140‑1 (MQ=255)
                                                                              ctctTCCAGCGAAACAGCAAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                            <  1:615839/140‑1 (MQ=255)
                                                                              ctctTCCAGCGAAACAGACAAATAGTCTTCCGGCCTTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                            <  2:3978874/140‑1 (MQ=255)
                                                                               tctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAc                                                           <  2:4824170/140‑1 (MQ=255)
                                                                                ctgccACCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                                          <  1:1111337/137‑1 (MQ=255)
                                                                                ctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                                          <  1:3726152/140‑1 (MQ=255)
                                                                                agtCCAGCGAAACAGCCAAATAGACTTCCGGCCGTGTCGATGTTTGAAATACTGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                                          <  2:4946044/138‑1 (MQ=255)
                                                                                  tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATTTTTTTTAAATAATTTTTTTTTGAATCTTTTTACAAGTGCAACAGTACATTTCGCAGATAAAACGACAGCTACCACTAAATAAAAAGGAATTGAATGAAACaa                                                        >  1:4729681/1‑139 (MQ=255)
                                                                                     aGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTATTTTTTGAATAAAGTTTTGGTGACTCTGTTTTCAATTGCATCTGTACAGTTGGCCGATAAAGAGACTGCTACCACAAAATAAAAAGGATTTGCATTAACCAggta                                                     >  1:1656754/1‑137 (MQ=255)
                                                                                                   aTAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGtgtg                                       <  2:5581059/140‑1 (MQ=255)
                                                                                                   aTAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGtgtg                                       <  2:2181796/140‑1 (MQ=255)
                                                                                                              gCCGTTTATATTTTTGAAATAATGTTTTTGTCACTCTGTTTAAAAGTGCATCTGTAAAATTCGCAGATAAAACGACATCTACCAAAAATTAAAAAGGATTGGAATTAAACACTTCACACAATGCGTGTTGAAACaattac                            >  2:6544912/1‑135 (MQ=255)
                                                                                                              gCCGTTTATATTTTTGAAATAATGTTTTTGTAACTATGTTTACAATTTCATCTGTACATTGCGCAGATAAAGCGAATGCTACCACTAATTCAAAAGGATTGTAATGAACCAGTTCACACACTGCGTGTGGAACCaaggag                            >  2:3773897/1‑135 (MQ=255)
                                                                                                              gCCGTGTATATTTTTGAAATAAAGTTTTGGTCACACTGTTTAAAAGTGCATCTGTACATTTCGCAGATAAAGCGAATGCTACCAATAATTAAAAAGGATTGGAATGAACCACTTCAACATCTGCGTGTGGAAACCTGTCg                            >  2:6812848/1‑140 (MQ=255)
                                                                                                               ccGTTTATATTTTTGAAATACTGTTTGGGTCACTCTCTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGc                           <  2:4242608/140‑1 (MQ=255)
                                                                                                               ccGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGc                           <  2:6269104/140‑1 (MQ=255)
                                                                                                               ccGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGc                           <  2:5951457/140‑1 (MQ=255)
                                                                                                                   ttATCTTTTTGACATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCGACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt                       <  2:4709577/140‑1 (MQ=255)
                                                                                                                   ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTCCATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt                       <  2:3868285/140‑1 (MQ=255)
                                                                                                                   ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt                       <  1:2579485/140‑1 (MQ=255)
                                                                                                                   ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt                       <  1:4187957/140‑1 (MQ=255)
                                                                                                                   ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt                       <  1:4841782/140‑1 (MQ=255)
                                                                                                                   ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt                       <  2:2867703/140‑1 (MQ=255)
                                                                                                                   ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt                       <  1:5062025/140‑1 (MQ=255)
                                                                                                                     atatTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACAGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCt                     <  2:2105121/140‑1 (MQ=255)
                                                                                                                     atatTTTTGAAATAATGTTTTGGACACTCTGTTTAAAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCt                     <  2:5108674/140‑1 (MQ=255)
                                                                                                                           tttGAAATAATGTTTGGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCt                <  2:4414928/140‑1 (MQ=255)
                                                                                                                            ttGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTca                                                    <  1:2010013/103‑1 (MQ=255)
                                                                                                                            ttGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTca                                                    >  2:2010013/1‑103 (MQ=255)
                                                                                                                              gAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGa             <  2:3385852/140‑1 (MQ=255)
                                                                                                                                        tttGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCaa   <  1:1014409/140‑1 (MQ=255)
                                                                                                                                         ttGGTGACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAAt  <  2:6635837/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
AATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAAT  >  NZ_CP034943/2214403‑2214678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: