BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,350,865	A→T	5.6%	pseudogene (916/1182 nt)	EO946_RS12020 ←	IS3 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,350,865	0	A	T	5.6%	1300.2 / 7.1	430	pseudogene (916/1182 nt)	EO946_RS12020	IS3 family transposase
Reads supporting (aligned to +/- strand): ref base A (107/285); new base T (26/3); total (140/290)
Fisher's exact test for biased strand distribution p-value = 2.31e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 432 total aligned reads displayed.

TTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCATTGTTGTAAAGATC  >  NZ_CP034943/2350727‑2351003

 tGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGACTGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGATATTCTTTTGTTGTGTAtt                                                                                                                                          >  1:7425174/1‑140 (MQ=255)

 tGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGTGATGGGTTTGTTGTGTAtt                                                                                                                                          >  2:2087289/1‑140 (MQ=255)

 tGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTAtt                                                                                                                                          >  2:1150180/1‑140 (MQ=255)

 tGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTGTTGGTTTGCGTTTGTTGTGTAtt                                                                                                                                          >  2:2192281/1‑140 (MQ=255)

 tGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTTTTTTTCTTGTGTTGTGTAtt                                                                                                                                          >  1:6894409/1‑140 (MQ=255)

 tGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGATTTTTAGATTCGTTTTTTGTGtttt                                                                                                                                          >  2:5128599/1‑140 (MQ=255)

 tGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGATGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGGGTTTGTTGTGtttt                                                                                                                                          >  1:5601539/1‑140 (MQ=255)

   aaaaaTATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTgg                                                                                                                                        <  2:1457137/140‑1 (MQ=255)

   aaaaaTATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTgg                                                                                                                                        <  2:6352998/140‑1 (MQ=255)

   aaaaaTATGCAGTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTgg                                                                                                                                        <  2:4908013/140‑1 (MQ=255)

        tatGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGcc                                                                                                                                   <  1:5969328/140‑1 (MQ=255)

          tGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTTTTATGCGTTTGTTGTGTTTTGGAAGCCtt                                                                                                                                 >  2:2635337/1‑140 (MQ=255)

             aTTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATTCTTTTTTTGTGTATTGGAAGCCTTGGt                                                                                                                              >  2:2122060/1‑140 (MQ=255)

             aTTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGt                                                                                                                              <  2:750792/140‑1 (MQ=255)

             aTTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGt                                                                                                                              <  1:4633938/140‑1 (MQ=255)

             aTTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGt                                                                                                                              <  2:5002284/140‑1 (MQ=255)

             aTTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGt                                                                                                                              <  2:3632611/140‑1 (MQ=255)

             aTTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGt                                                                                                                              <  2:4174984/140‑1 (MQ=255)

             aTTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGATATGCGTTTGTTGTGTATTTGAAGCCTTGGt                                                                                                                              >  2:5464666/1‑140 (MQ=255)

             aTTCTCTGTTTTGAGATGCGAGAAAAACCATTCGAGACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGt                                                                                                                              <  2:6775333/140‑1 (MQ=255)

               tctcTGTTTTGAGATGCGAGAAAAACCATTCGATACATGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGATTTTGATATGCTTTTGTTGTGTATTGTAAGCCTTGGTCa                                                                                                                            <  2:3435798/140‑1 (MQ=255)

               tctcTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCa                                                                                                                            <  1:4695123/140‑1 (MQ=255)

                  ctGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGaa                                                                                                                         <  2:3554391/140‑1 (MQ=255)

                  ctGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGaa                                                                                                                         <  2:585481/140‑1 (MQ=255)

                    gTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTTATATTCTTTTGTTGTGTAATTGAAGCCGTGTTGAGAAtt                                                                                                                       >  2:7110704/1‑139 (MQ=255)

                    gTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATg                                                                                                                       <  1:7254813/140‑1 (MQ=255)

                    gTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATg                                                                                                                       <  1:509797/140‑1 (MQ=255)

                            aTGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAg                                                                                                               <  2:2019355/140‑1 (MQ=255)

                            aTGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAg                                                                                                               <  2:5428555/140‑1 (MQ=255)

                             tGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTTTTATTGTTTTTTTTTTTATTTGGATCCGTGGGGAGAATTGAGGGTAGc                                                                                                              >  1:1365618/1‑140 (MQ=255)

                               cGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTTTTATTGTTTTGTTTTGTTTTGTAATCCGATGTGAGAAATGAGGGTAGCTc                                                                                                            >  1:4212059/1‑140 (MQ=255)

                               cGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTTTTATTGTTTTTTTGTGTTTTTGAAGCCGTGGTCAGAATGGAGGATatggc                                                                                                            >  1:2223191/1‑136 (MQ=255)

                                 agaAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTTATATTGGTTTTTTGTTTATTTTAAGCCATTGTGTGAAAGGAGGATatatact                                                                                                          >  2:1331592/1‑134 (MQ=255)

                                 agaAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATTGTTTTTTTGTGGGTTGGTAGCCGTTGTGCGAATGGAGGATAGCGCCt                                                                                                          >  2:2455771/1‑140 (MQ=255)

                                 agaAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATTCTTTTTTTGTGTATTTGAAGCCTTGGGGAGAATTGAGGATAgctgct                                                                                                          >  2:2774246/1‑140 (MQ=255)

                                       aCCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACg                                                                                                    <  1:5004970/140‑1 (MQ=255)

                                       aCCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACg                                                                                                    <  1:1043404/140‑1 (MQ=255)

                                       aCCATTCGATACAGGCATTATCTAGGCAGGTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACg                                                                                                    <  2:7282605/140‑1 (MQ=255)

                                          aTTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTTTTATTGGTTTGTTTTGTTTTGTAAGCCTTGGGGAGTATTGTGGATAGATCCTTGGACGTcc                                                                                                 >  1:5730257/1‑140 (MQ=255)

                                               aTACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCACCGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTg                                                                                            <  1:1723673/140‑1 (MQ=255)

                                               aTACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTg                                                                                            <  1:4114195/140‑1 (MQ=255)

                                               aTACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTg                                                                                            <  1:3696045/140‑1 (MQ=255)

                                               aTACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTg                                                                                            <  1:3482943/140‑1 (MQ=255)

                                                 aCAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTggg                                                                                          <  1:5401132/140‑1 (MQ=255)

                                                     gCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTTGTATTCTTTTTTTGTGTATTGGAAGCCTTGGTCAGAATTGAGGTTAGCTGCGTGGTCGTGCCGGTggggggt                                                                                      >  2:7187856/1‑136 (MQ=255)

                                                     gCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTTTTATTGTTTTTTTTTGTTTTTGTATCCGTTGTGAGAATTGTGGATAGGGGCTTGGACGTGCCCGTGGGgtgt                                                                                      >  1:3873943/1‑140 (MQ=255)

                                                      cATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCACCGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTc                                                                                     <  1:2635337/140‑1 (MQ=255)

                                                      cATTATCTAGGCAGTTTGCCTGACGGTAATGGCTGCCTTTCAACCCAACGGTTTCGAGTCTCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTc                                                                                     <  2:4745497/140‑1 (MQ=255)

                                                      cATTATCTAGGCAGTTGGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTc                                                                                     <  1:825372/140‑1 (MQ=255)

                                                        ttATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCAt                                                                                   <  2:2499095/140‑1 (MQ=255)

                                                        ttATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGGCGCTTTTGATATGCTTTTGTTGTGTATAGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCAt                                                                                   <  1:2912907/140‑1 (MQ=255)

                                                          aTCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTTTTATTCTTTTGTTGTGTTTTTGAATCCATGGTGACAATGGAGGATAGCGCCTTTGACGTACCGTTGGGCTGTCATAc                                                                                 >  2:2924993/1‑140 (MQ=255)

                                                                gCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGTAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCa                                                                           <  2:1822783/140‑1 (MQ=255)

                                                                gCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCa                                                                           <  2:5233443/140‑1 (MQ=255)

                                                                gCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCa                                                                           <  1:1577507/140‑1 (MQ=255)

                                                                gCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCa                                                                           <  1:704414/140‑1 (MQ=255)

                                                                gCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCa                                                                           <  1:2007890/140‑1 (MQ=255)

                                                                  aGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACt                                                                         <  1:952278/140‑1 (MQ=255)

                                                                   gTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGTTATGCTTTTGTTGGTTATTGGAAGCCTTGGTCAGAATGGGGGATAGCTCCATGGACGTCCCGGTTGGCTGTGATAATTTacacag                                                                        >  2:10712/1‑138 (MQ=255)

                                                                   gTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTg                                                                        <  1:5188973/140‑1 (MQ=255)

                                                                   gTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTg                                                                        <  1:6803190/140‑1 (MQ=255)

                                                                   gTTTGCCTTACGGGAATGGCTCCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTGGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTg                                                                        <  2:4428982/140‑1 (MQ=255)

                                                                    tttGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGTTATGCTTTTTTTTTGTATTGGAAGCCTTTGTCAGAATTGAGGATAGCTACTTTGAAGTCCCCTTTGGGTGTCATAAGTTACACTGt                                                                       >  2:3077924/1‑140 (MQ=255)

                                                                    tttGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTTTTGTGTTTTTGAAGCCTTTGTCAGAATGGACGCTTTCGCCGTGGACGTGCCGGTGGGGTGTCATACtttacccagt                                                                       >  2:3850580/1‑133 (MQ=255)

                                                                    tttGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGATTTTGTTTTGTATTGGAAGCCTTGGTCATAATTGAGGATAGCTCCATGGACGTCCCGATGGGGTGTCATACTTTCCACTGt                                                                       >  2:5647335/1‑140 (MQ=255)

                                                                    tttGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAATCTTTTGTTGTGTATTGGAACCCATGGTCACAATTGAGGATAGCTCCATGGACGTCCCCTTGGGCTGTCAAAATTTACACAGt                                                                       >  2:6232393/1‑140 (MQ=255)

                                                                        ccTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGAGAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTc                                                                   <  1:1624043/140‑1 (MQ=255)

                                                                             cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                                              <  1:1012032/140‑1 (MQ=255)

                                                                             cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                                              <  2:7658882/140‑1 (MQ=255)

                                                                               ggAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCa                                                            <  2:740532/140‑1 (MQ=255)

                                                                                  aTGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGtt                                                         <  1:1150180/140‑1 (MQ=255)

                                                                                   tGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATTCTTTTGGTGTGTATTGGAAGCCGTGGTCAGAATTGAGGGTAGCTGCTTGGACGTCCCGTTGGGCTGTCATACTTTGCACGGTGTTAATGACCACTTc                                                        >  2:4291738/1‑140 (MQ=255)

                                                                                           tttCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTTTTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCAt                                                <  2:1594166/140‑1 (MQ=255)

                                                                                                 aCCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGtt                                          <  2:4669824/140‑1 (MQ=255)

                                                                                                      cGGTTTCGAGTCGCTTTTGATATGCTATTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGAc                                     <  1:1882634/140‑1 (MQ=255)

                                                                                                                tCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATg                           <  1:437687/140‑1 (MQ=255)

                                                                                                                   cTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGa                        <  2:2297748/140‑1 (MQ=255)

                                                                                                                    ttttGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGAc                       <  2:124246/140‑1 (MQ=255)

                                                                                                                         atatGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAAtct                  <  1:4254843/140‑1 (MQ=255)

                                                                                                                         atatGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAAtct                  <  2:4024836/140‑1 (MQ=255)

                                                                                                                           atGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCa                <  1:639977/140‑1 (MQ=255)

                                                                                                                           atGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCa                <  1:3260869/140‑1 (MQ=255)

                                                                                                                                   gttgtGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCATTGTTGTa        <  2:5315860/140‑1 (MQ=255)

                                                                                                                                   gttgtGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCATTGTTGTa        >  1:7624583/1‑140 (MQ=255)

                                                                                                                                    ttgtGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCATTGTTGTaa       <  2:5587118/140‑1 (MQ=255)

                                                                                                                                    ttgtGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCATTGTTGTaa       <  2:3247034/140‑1 (MQ=255)

                                                                                                                                    gtgtgTATTGGACGCCTTGGACAGAATGGAGGATCGCTCCTTGGCCGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTGTGACAGCTTCCATGCGACAATCTCATTGTTGTaa       <  1:3598587/139‑1 (MQ=255)

                                                                                                                                    gtgtgTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCATTGTTGTaa       <  1:6206219/139‑1 (MQ=255)

                                                                                                                                       tgtATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                                              <  1:7170506/82‑1 (MQ=255)

                                                                                                                                       tgtATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCATTGTTGTAAAGa    <  2:5770975/140‑1 (MQ=255)

                                                                                                                                         tATTGGACGCCGTGGTCAGAAGGGAGGATAGCTCCTTGGACGTCCCGGTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCATTGTTGTAAAGATc  <  2:7042247/140‑1 (MQ=255)

                                                                                                                                         tATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                                              >  2:6534912/1‑80 (MQ=255)

                                                                                                                                         tATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                                              <  1:6534912/80‑1 (MQ=255)

                                                                                                                                         tATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCAt                                                >  1:4534046/1‑94 (MQ=255)

                                                                                                                                         tATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCATTGTTGTAAAGATc  >  2:6893243/1‑140 (MQ=255)

                                                                                                                                         tATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCATTGTTGTAAAGATc  >  2:2822458/1‑140 (MQ=255)

                                                                                                                                         tATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCATTGTTGTAAAGATc  >  1:2557230/1‑140 (MQ=255)

                                                                                                                                         tATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCATTGTTGTAAAGATc  >  2:606892/1‑140 (MQ=255)

                                                                                                                                         tATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCATTGTTGTAAAGATc  >  2:7757238/1‑140 (MQ=255)

                                                                                                                                         tATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCATTGTTGTAAAGATc  >  2:926167/1‑140 (MQ=255)

TTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGTCCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCATTGTTGTAAAGATC  >  NZ_CP034943/2350727‑2351003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`