Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,387,114 C→A 8.3% V338L (GTA→TTA)  EO946_RS12195 ← acetyl‑CoA C‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,387,1140CA 8.3% 997.5 / 21.7 399V338L (GTA→TTA) EO946_RS12195acetyl‑CoA C‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (192/155);  new base A (43/3);  total (240/159)
Fisher's exact test for biased strand distribution p-value = 9.44e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 400 total aligned reads displayed.

GAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCAAACCCCGGAGC  >  NZ_CP034943/2386976‑2387252
                                                                                                                                          |                                                                                                                                          
 aGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTTATTCGTAAACAAGTGTCCTGAGTATTGTCGCACCGCGTGCGCCGATCGGGTGGGCGAGGGCGAGCGCCACACCGGGTTCAATTACtt                                                                                                                                          >  1:4376981/1‑140 (MQ=255)
      cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCa                                                                                                                                     >  2:4389162/1‑140 (MQ=255)
      cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGGTTACATTTACtttttaa                                                                                                                                     >  2:1149323/1‑138 (MQ=255)
      cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCGTGCGCCGATCGCGTGGCCGAGGGCGCTCGCACCACCGGTTACATTTACTTTTTCa                                                                                                                                     >  2:7151130/1‑140 (MQ=255)
      cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTAATGAGTATTCTCGCACCGCGTGCGCCGATCCGGTGGCCGAGGGCGATAGCACCACCGGTTAAATTTAAtttttaa                                                                                                                                     >  2:4962395/1‑138 (MQ=255)
      cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGGAAACCAGGGTCATGAGTATTCTCCCCCCGCGTGCGCCGATCGGGGGGCCGAGGGCGATCGCACCACCGTGTACCTTTACtttttaa                                                                                                                                     >  2:1247552/1‑138 (MQ=255)
      cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAAGTCGTAAACCAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTAATTTTTCa                                                                                                                                     >  2:3779471/1‑140 (MQ=255)
      cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTTATTCGTAAACAACTGTGATGAGTATTCTGGCACCCCGTGCGCCGCTCGGGTGGGCGCGGGCGATCGCCCCACCCGTTACATTTTAtttttta                                                                                                                                     >  1:6473022/1‑138 (MQ=255)
      cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTAGTAAACAAGTGTCATGAGTAATCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGGTTAACATTAAtttttaa                                                                                                                                     >  2:2383775/1‑138 (MQ=255)
      cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGGCGCGTTATTTCGTTAAACAGTGTCATGAGGATTCTCGGACCGCTTGGGGCGATCGGGTGGGCGAGGGCGGTCGCACCACCGGTGGCCTTTTCTTTTTCa                                                                                                                                     >  1:6046006/1‑140 (MQ=255)
           cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTAGTAAAAAACTGTCATGAGTAATATGGCACCCCATGGGCCGATCGGGTGGCCGAGGGCGAGGGCCCCCCCGTTTACATTTACTTTTTGAAGATc                                                                                                                                >  1:2166395/1‑140 (MQ=255)
           cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTAGTAAACAAGTGTGATGAGGATTCTAGCACCGCGTGCGCCCATCGGGGGGCCGAGGGCGCTCGCACCACCCGTTACATTTAATTTTTAAAGAta                                                                                                                                >  1:3810836/1‑139 (MQ=255)
                aaTGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAACACGTGTCATGAGTATTCTCGCCCCCCATGCGCCCATCGGGTGGCCGAGGGCCATCGCACCACCCGTTCAATTTACATTTTAAAAATCGAAAc                                                                                                                           >  2:6448059/1‑140 (MQ=255)
                aaTGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAACAAGGGTCATGAGTATTCTCGCACCCCGTGCGCCGGGCGGGTGGCCGTGGGCGATCGCACCCCCCGTTCCCATTAAATTTTCAATATCGAAAc                                                                                                                           >  1:7417575/1‑140 (MQ=255)
                   ggcggcAACACCCAGTCCCCCTCCCCGTCGCTTTAATTAGTAAACAAGTGTCATGCGTATTCTCGCACCGCTTGCGCCGATCGGGTTGCCGAGGGCGCACGCACCACCGGTTACATTTACTTTTTAAAGATCGAAACcgc                                                                                                                        >  2:2796355/1‑139 (MQ=255)
                      ggcAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAACAAGTGTCAAGGGTATTCTCGCACCGCGTGCGCCGCGCGGGTGGCCGAGGGCGCTCGCACCACCCGTTACATTTACTTTTTCAAGATCGAAACcgacga                                                                                                                     >  2:1877794/1‑140 (MQ=255)
                              ccAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTa                                                                                                             <  1:2566378/140‑1 (MQ=255)
                              ccACTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTa                                                                                                             <  1:6817754/140‑1 (MQ=255)
                                      ccTCCCCGTCGCTTTAATTCGTAAAAAATTGTCATTAGTATTCTCGCACCGCTTGCGCCCATCGGGTGGCCGAGGGCGATCGCACCCCCGTTTACATTTTCTTTTTAAAGATCGAAACCGACGATTTTTTTAAATGTCaa                                                                                                     >  2:4569362/1‑140 (MQ=255)
                                            cGTCGCGTTAATTCGTAAAAAAGTGTGATGTGTATTGTCGCACCGCGTGCGCCGATGGGGTGGCCGAGGGCGATCGCACCACCGGTTACATTTACTTTTTGAAGATCGAAACCGACGATTTTTTTACATGTGAAaacaaa                                                                                               >  2:2140474/1‑139 (MQ=255)
                                                   ttAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCa                                                                                        <  2:7338839/140‑1 (MQ=255)
                                                    tAATTCCTAAACAACTGTCATGTGTATTCTGGCACCGCATGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGGTTACATTTACTTTTTCAAGATCGAAACCTACGATTTTTTTGCATGTCAAAACAACAGAAGccc                                                                                       >  2:6702535/1‑138 (MQ=255)
                                                           tAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTc                                                                                >  2:1921267/1‑140 (MQ=255)
                                                           tAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTc                                                                                >  2:6520044/1‑140 (MQ=255)
                                                            aaaCAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCa                                                                               >  2:3126715/1‑140 (MQ=255)
                                                             aaCAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCAt                                                                              <  2:6149062/140‑1 (MQ=255)
                                                             aaCAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCAt                                                                              <  2:3853430/140‑1 (MQ=255)
                                                             aaCAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCAt                                                                              <  2:782207/140‑1 (MQ=255)
                                                                aaGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTCCATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTa                                                                           >  2:2966023/1‑140 (MQ=255)
                                                                     tCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACTACAGAAGCAAACGCTTCATTTACTTCa                                                                      >  2:4770044/1‑140 (MQ=255)
                                                                     tCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCa                                                                      >  2:5725321/1‑140 (MQ=255)
                                                                       aTGAGTATTCTCGCACCGCTTGGGCAGATCGGGTGGCCGAGGGCGATCGCACCACCTTTTACATTTAGTTTTTCATGATCGAAACCGACGATTTTTTTTCATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTaaaa                                                                    <  1:943897/140‑1 (MQ=255)
                                                                       aTGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCaaa                                                                    <  1:607008/140‑1 (MQ=255)
                                                                       aTGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCaaa                                                                    <  1:1055970/140‑1 (MQ=255)
                                                                       aTGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCaaa                                                                    <  1:2048580/140‑1 (MQ=255)
                                                                          aGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACaa                                                                 >  2:6363913/1‑140 (MQ=255)
                                                                          aGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAAAGCTTCATTTAATTCAAACaa                                                                 >  2:3517845/1‑140 (MQ=255)
                                                                            tATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGt                                                               >  1:7111956/1‑140 (MQ=255)
                                                                            tATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGt                                                               >  1:552361/1‑140 (MQ=255)
                                                                            tATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGt                                                               >  2:142056/1‑140 (MQ=255)
                                                                            tATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGt                                                               >  1:6391503/1‑140 (MQ=255)
                                                                            tATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGt                                                               >  1:1659615/1‑140 (MQ=255)
                                                                            tATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTGTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGt                                                               >  2:1693596/1‑140 (MQ=255)
                                                                              ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCACGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACCAGTCa                                                             <  1:5759272/140‑1 (MQ=255)
                                                                              ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                             <  1:1791066/140‑1 (MQ=255)
                                                                              ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                             <  1:6901344/140‑1 (MQ=255)
                                                                              ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                             <  2:3653574/140‑1 (MQ=255)
                                                                              ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                             >  2:6042530/1‑140 (MQ=255)
                                                                              ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                             <  1:4419590/140‑1 (MQ=255)
                                                                              ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                             <  1:2777290/140‑1 (MQ=255)
                                                                              ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                             <  1:3126715/140‑1 (MQ=255)
                                                                              gtctcGCACCGCGTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                             <  1:2307090/139‑1 (MQ=255)
                                                                               tctcGCACCGCTTGCGCCGATCGGGTTGCCGATGGCGATCGAACCAACTTTTAAATTTACTTTTTCAATATCTAAAAATAAGATTTTTTTACATGTCAAAACATCTGAAGAAAAATATTCATTTACTTCAAACAAGTaaa                                                            <  2:2224288/140‑1 (MQ=255)
                                                                               tctcGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCaa                                                            >  1:5563360/1‑140 (MQ=255)
                                                                                 tcGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAata                                                          >  1:7575704/1‑140 (MQ=255)
                                                                                 tcGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAata                                                          >  1:5910377/1‑140 (MQ=255)
                                                                                      ccGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTg                                                     <  2:5961671/140‑1 (MQ=255)
                                                                                      ccGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTg                                                     >  1:7046143/1‑140 (MQ=255)
                                                                                        gcgtGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGa                                                   <  2:7617934/137‑1 (MQ=255)
                                                                                        gCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGa                                                   <  2:558510/140‑1 (MQ=255)
                                                                                        gCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGa                                                   <  2:3270333/140‑1 (MQ=255)
                                                                                        gCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGa                                                   <  2:2067455/140‑1 (MQ=255)
                                                                                        gCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGa                                                   <  2:7111956/140‑1 (MQ=255)
                                                                                        gCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGa                                                   <  1:4220798/140‑1 (MQ=255)
                                                                                        gCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGa                                                   <  1:3232271/140‑1 (MQ=255)
                                                                                        gCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGa                                                   <  1:2619384/140‑1 (MQ=255)
                                                                                        gCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGa                                                   <  1:126886/140‑1 (MQ=255)
                                                                                        gCTTGCGCCGATCGGGGGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGa                                                   <  2:6796673/140‑1 (MQ=255)
                                                                                         cTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGa                                                   <  1:3410177/139‑1 (MQ=255)
                                                                                         cTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGAc                                                  >  1:7686042/1‑140 (MQ=255)
                                                                                         cTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGAc                                                  >  1:250203/1‑140 (MQ=255)
                                                                                           tGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACgg                                                <  2:1618300/140‑1 (MQ=255)
                                                                                           tGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACgg                                                >  1:5063130/1‑140 (MQ=255)
                                                                                                 gATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAAc                                          >  2:5864801/1‑140 (MQ=255)
                                                                                                 gATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAAc                                          >  2:5325558/1‑140 (MQ=255)
                                                                                                  aTCGGGTGGCCGAGGTCGATCGCACCACCGTTTAGATTTACTTTTTCAAGATCGAAACCGTCGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAAcc                                         >  1:1162728/1‑140 (MQ=255)
                                                                                                  aTCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTAATTCAAACAAGTCAATATCTTGGACGGTTAAAcc                                         >  2:5454375/1‑140 (MQ=255)
                                                                                                  aTCGGGTGGCCGAGGGCGATCGCACCACCGATTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTCCTTCAAACAAGTCAATATCTTGGACGGTTAAAcc                                         >  1:515194/1‑140 (MQ=255)
                                                                                                   tCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCAt                                                                              >  2:2524562/1‑102 (MQ=255)
                                                                                                      ggTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTc                                     <  1:1580340/140‑1 (MQ=255)
                                                                                                       gTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCACTATCTTGGACGGTTAAACCGTTCt                                    >  2:4299647/1‑140 (MQ=255)
                                                                                                       gTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCt                                    >  2:886303/1‑140 (MQ=255)
                                                                                                       gTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCt                                    <  2:6241096/140‑1 (MQ=255)
                                                                                                            cGAGGGCGATCGCACCACCGTGTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTc                               >  2:270066/1‑140 (MQ=255)
                                                                                                            cGAGGGCGATCGCACCACAGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAAAGCTTCATTTACTTCAAACAAGTCAAAATCTGGGACGGTTAAAGCGTTATATTTc                               >  2:6322019/1‑140 (MQ=255)
                                                                                                             gAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAACTAAATATCTTGGACGGTTAAACCGTTCTTTTTCa                              <  2:3424680/140‑1 (MQ=255)
                                                                                                                    aTCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCtt                       >  2:7238202/1‑140 (MQ=255)
                                                                                                                     tCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAAc                                          >  2:7423296/1‑120 (MQ=255)
                                                                                                                        caccacAGTTTAAATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTa                   <  2:4733661/140‑1 (MQ=255)
                                                                                                                             ccGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGc              >  2:6969432/1‑140 (MQ=255)
                                                                                                                              cGTTTACATTTACTTTTTCACGATCGCAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCa             <  2:6005055/140‑1 (MQ=255)
                                                                                                                               gTTCACATTTACTATTTCAAGATCGAAACCGAAGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCaa            <  1:4713686/140‑1 (MQ=255)
                                                                                                                                   aCATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCAAAccc        >  1:4169219/1‑140 (MQ=255)
                                                                                                                                   aCATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTGCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCAAAccc        >  2:13161/1‑140 (MQ=255)
                                                                                                                                     aTTTACTTTTTCAAGATCGAAACCTACGATTTTTTCACATGTCAAAACAACTGAAGCAAAAGCTTCATTTACTTCAAACAAGTCAATATCTTGGACAGTTAAACCATTCTTTTTCAGGAGCTTGTTAATAGCAAAccctg      >  2:7571575/1‑138 (MQ=255)
                                                                                                                                       ttACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCAAACCCCGGa    >  2:1567020/1‑140 (MQ=255)
                                                                                                                                       ttACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCAAACCCCGGa    >  2:5142324/1‑140 (MQ=255)
                                                                                                                                       ttACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTATGACGGTTAAACCGTTCTTTTTCAGGAGCTTTTTAATTGAAAACCCCGGa    >  2:4061065/1‑140 (MQ=255)
                                                                                                                                       ttACTTTTTCAAGATCGAAAAAGAATATTTTAAAACATGTCAAAACAACATAAGCAAACGCTTCATTTACTTCAAAAAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTAAGGAGCTTGTTAATTGAAAACCCCGGa    >  2:1269761/1‑140 (MQ=255)
                                                                                                                                         aCTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCAAACCCCGGAGc  <  2:5049235/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
GAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCAAACCCCGGAGC  >  NZ_CP034943/2386976‑2387252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: