Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,729,932 C→A 7.2% M278I (ATG→ATT lcfA ← long‑chain‑fatty‑acid‑‑CoA ligase LcfA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,729,9320CA 7.2% 1220.4 / 17.8 453M278I (ATG→ATTlcfAlong‑chain‑fatty‑acid‑‑CoA ligase LcfA
Reads supporting (aligned to +/- strand):  ref base C (103/296);  new base A (51/2);  total (154/299)
Fisher's exact test for biased strand distribution p-value = 1.75e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 462 total aligned reads displayed.

GATGACAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTATTGGCCA  >  NZ_CP034943/2729795‑2730071
                                                                                                                                         |                                                                                                                                           
gATGACAGATCATAATGCTGCCATTCGGGGTGATGCAAAGGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTGTTTTTCGATGAGTTTACGCGTTTTGTGCGGATAAAAAATAGGGCGTAGAATCAt                                                                                                                                           >  1:7766726/1‑140 (MQ=255)
 aTGACAGATCATCATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCCTTTCGAGCGGATCAAACGTATGAAGAAGAATCAtt                                                                                                                                          >  2:1768913/1‑140 (MQ=255)
 aTGACAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTTTCGATGATTTTAAGCGTTTAGAGCGGATCAAACTTAGGAAGAAAAATAAtt                                                                                                                                          >  2:1483409/1‑140 (MQ=255)
    aCAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCg                                                                                                                                       <  1:5483633/140‑1 (MQ=255)
    aCAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCg                                                                                                                                       <  1:4993289/140‑1 (MQ=255)
        aTCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTTTGGATGATTTTGAGCGTTTGGCGCGGATCAAACTTTGGAAGAAGAATCATTTAGTAGc                                                                                                                                   >  1:6851652/1‑140 (MQ=255)
         tCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGcc                                                                                                                                  <  2:5454044/140‑1 (MQ=255)
         tCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGcc                                                                                                                                  <  2:2905142/140‑1 (MQ=255)
         tCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGcc                                                                                                                                  <  2:6233698/140‑1 (MQ=255)
         tCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGcc                                                                                                                                  <  2:7314112/140‑1 (MQ=255)
         tCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGcc                                                                                                                                  <  1:6990163/140‑1 (MQ=255)
         tCATAATGCTGCAAGTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGcc                                                                                                                                  <  1:89277/140‑1 (MQ=255)
           aTAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCTGTGAACCCGGAAAGAGAGTCGGTTTTTGTTTTTCGATTATTTTAAGCGTTTAGAGCGGATCAAAAATAGGAACAAGAATAATTTTGaataata                                                                                                                                >  2:6079179/1‑135 (MQ=255)
                  tgcAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTTTCGTTGATTTTAACCGTTTTGTGCGGATCAAAATTAGGGATAAGAAAAAATTAGAAGCCTAGttttaa                                                                                                                         >  1:6624019/1‑137 (MQ=255)
                  tgcAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTTTAGATGATTTTGAGCGTTTGGAGCGGATAAAAAGTAGGAATAATAATCATTTATAATACTAGTTTAAt                                                                                                                         >  1:3296775/1‑140 (MQ=255)
                  tgcAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTGGATTTTTTTCAGCGTTTCGAGCGGATCAAAATTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAAt                                                                                                                         >  2:1568895/1‑140 (MQ=255)
                  tgcAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTGTTTTTGGATGTTTTTCATCGTTTAGTGCGGAAAAAAATTAGGAAAAAGAATCAATTAGAAGCCAAGTTTCAt                                                                                                                         >  1:5229983/1‑140 (MQ=255)
                    cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTTTGGATGATTTTAAGCGGTTCGAGCGGATCAAAATTATGAAGAAAAATAAATTCGAAGCCTATTTTAAACg                                                                                                                       >  2:3566526/1‑140 (MQ=255)
                    cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTTTCGATGATTTTAAGCGTTTGGAGCGGATCAAACTTAGGAAGAAGAATAAATTCGAAGGCTAGTTTAAtag                                                                                                                       >  2:2296479/1‑138 (MQ=255)
                    cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTGGATTATTTTCAGCGTTTGGTGCGGATCAAACTTATGTAGAAGAATCATTTGGAAGCCGAGTTTAAtag                                                                                                                       >  1:7301023/1‑138 (MQ=255)
                    cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGAATATTTTGAGCGTTTCGAGCGGATCAAAATTAGGAAAAAGAAACATTTAGAAGGCTAGTTTAAtag                                                                                                                       >  2:3347599/1‑138 (MQ=255)
                    cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTTTTTGGCGATGATTTTCATCGTTTAGAGCGGATCAAAAATAGGAAGAAGAATCATTTGGAAGCCGAGGTTAATCg                                                                                                                       >  2:4028749/1‑140 (MQ=255)
                    cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTGTTTGTCGATGATTTTAAGCGTTTCGAGCCGATCAAAATTATGAAGAATAATAATTTCGAAGCCTAGTTTAATCg                                                                                                                       >  2:532158/1‑140 (MQ=255)
                         cGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGaataa                                                                                                                  <  1:558557/140‑1 (MQ=255)
                         cGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGGTTCGAGCGGATCAAAATTATGAAGAAGAATCATTTAGAAGCCTAGTTTAATCGaataa                                                                                                                  >  2:7020054/1‑140 (MQ=255)
                           gggTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTTTGTGTCGATGATTTTAAGCGTTTGGAGCGGCTGAAACTTAGGAAGAACAATCATTTAGAAGCCGAGTGTAATCGCATAAtt                                                                                                                >  1:2074580/1‑140 (MQ=255)
                           gggTGATGCAAAAGGCCAATGTAAATGGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAAtt                                                                                                                <  1:1674531/140‑1 (MQ=255)
                            ggTGATGCAAAAGGCCAATGTCCATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTTTTTGTCGATGATTTTGCGCGGTTCGAGCGGATGAAACTTAGGAAGAAGTAAACTTTAGAAGCCGAGTTTAATCGAATAATtc                                                                                                               >  1:3637741/1‑140 (MQ=255)
                            ggTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTTTCGCTGATTTTAAGCGTTTAGATCGGATAAAACGTTGGAAGAACAAAAATTTCGAAGCCTAGTTTAATCGaaaaatac                                                                                                               >  2:4172978/1‑138 (MQ=255)
                                aTGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTGTTTGTCGATGATTTTGAGCGTTTAGAGCGGATCAAACTTAGGAAGAAGAAACATTTCGTAGCCGAGTTTAATCGAATAATtcatca                                                                                                           >  1:3071042/1‑140 (MQ=255)
                                 tGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTTTTTTTCGATTATTTTAAGCGTTTAGAGCGGATGAAACTTATGAAAAAGAATAAATTAGAATCCTAGTTTAATAGAAAAAttaaaaac                                                                                                          >  1:3904238/1‑134 (MQ=255)
                                 tGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTTTTTTTCGATGATTTTGAGCGTTTGGAGCGGGTCAAACTTAGGCAGAAGAATAAATTAGAAGCCTGGTTTAATCGAATAATTCAacac                                                                                                          >  2:714805/1‑140 (MQ=255)
                                 tGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCAc                                                                                                          <  1:6752466/140‑1 (MQ=255)
                                 tGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCAc                                                                                                          <  1:5912470/140‑1 (MQ=255)
                                 tGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTGTTTGTCGATGATTTTGAGCGTTTAGAGCGGCTAAAAAATATGAAGAACAATAAATTCTAAGACGAGTTTAATCGAATAATTAATAAc                                                                                                          >  1:7358923/1‑140 (MQ=255)
                                      aaGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTTTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTAGAAGCCGAGGTTAATCGAATAATTCATCACAGCCg                                                                                                     >  2:1363020/1‑140 (MQ=255)
                                      aaGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCg                                                                                                     <  1:1386358/140‑1 (MQ=255)
                                       atgCCAATGTAAATTGTCGGTGCAACCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCTGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGt                                                                                                    <  1:4653251/138‑1 (MQ=255)
                                       aGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGt                                                                                                    <  2:3810035/140‑1 (MQ=255)
                                       aGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGt                                                                                                    <  1:6499331/140‑1 (MQ=255)
                                         gCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCa                                                                                                  <  1:7577481/140‑1 (MQ=255)
                                           cAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGAACAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGt                                                                                                <  2:1131722/140‑1 (MQ=255)
                                           cAATGTAAAGTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTGGTCGATGAATTTCAGCGTTTCGAGCGGATCAAACTTTGGTAGACGAATCATTTCGACGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGt                                                                                                <  2:421552/140‑1 (MQ=255)
                                             aTGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTGTTTTTTTGGATGTTTTTAAACCTTTCGAGCGGCACAAAATTAGGAATAATAATAAATAAGAACGCTAGTTTAATAGAATAAATAATAACTTcagtcagtac                                                                                              >  1:614726/1‑138 (MQ=255)
                                                   aTTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTTTGAAGAAGAATCAATTCGAAGCCTAGTTTAATCGAATAATTCATAACTTCCGTCAGTCCGtgaaa                                                                                        >  2:7555078/1‑136 (MQ=255)
                                                        cGGTGCACCCGGAAAGAGAGTCGGGTTTTGTTTGTGGATTAATTTGAGCGTTTCGAGCGGATGAAAATTAGGAAAAACAATAATTTCGAAGCCTATTTTAATTGACAAATAAATGACTTCCGTGAGTGCGTATAAGTgta                                                                                   >  2:4746684/1‑138 (MQ=255)
                                                                 cGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGa                                                                          <  1:5780846/140‑1 (MQ=255)
                                                                   gAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACg                                                                        <  1:1567246/140‑1 (MQ=255)
                                                                   gAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACg                                                                        <  1:4199166/140‑1 (MQ=255)
                                                                   gAAAGAGAGTCGGTTTTTGTGTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACg                                                                        <  1:4384133/140‑1 (MQ=255)
                                                                         gagTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGccc                                                                  <  1:636601/140‑1 (MQ=255)
                                                                            tCGGTTTTTGTTTGTCGATTATTTTAATCGGTTCGAGCGGATCAAACTTAGGAAGAAGAAACATTTAGCACCCAAGTTTTATCGAAATATTAATCACATCCCTCAGTCCGTATACGTTAAAAAACCGGAAGATGcacacc                                                               >  2:7660054/1‑138 (MQ=255)
                                                                             cGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCa                                                              <  1:3744474/140‑1 (MQ=255)
                                                                              gggttttGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAATAAGAATCATTTAGAATCATAGTTTAATCGAAAAAATCAAAAAATCCGTCCGTCCGTATAAGTGAAAAAAAGGGACGATGCCCAGCAc                                                             >  2:4461795/4‑140 (MQ=255)
                                                                                   ttgtttgtCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACttttt                                                        <  1:3515119/140‑1 (MQ=255)
                                                                                      tgtgtCGATGATTTTCGGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAg                                                     <  1:7489945/138‑1 (MQ=255)
                                                                                       ttgtCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGc                                                    <  1:2787919/140‑1 (MQ=255)
                                                                                        tgtCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcc                                                   <  2:427149/140‑1 (MQ=255)
                                                                                        tgtCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcc                                                   <  2:855783/140‑1 (MQ=255)
                                                                                        tgtCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcc                                                   <  1:2466624/140‑1 (MQ=255)
                                                                                        tgtCGATGATATTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGACTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcc                                                   <  1:4461795/140‑1 (MQ=255)
                                                                                          tCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcccc                                                 <  1:1483409/140‑1 (MQ=255)
                                                                                             atgatTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCccttc                                              <  2:5024062/140‑1 (MQ=255)
                                                                                                atTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCccttcctt                                           <  1:100808/140‑1 (MQ=255)
                                                                                                  tttCAGCGTTTCGAGCGTATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCTAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTca                                         <  2:7250741/140‑1 (MQ=255)
                                                                                                  tttCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTca                                         <  1:6773022/140‑1 (MQ=255)
                                                                                                  tttCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTca                                         <  2:3244211/140‑1 (MQ=255)
                                                                                                  tttCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTca                                         <  1:5336304/140‑1 (MQ=255)
                                                                                                    tCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTcaca                                       <  1:5148535/140‑1 (MQ=255)
                                                                                                    tCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTcaca                                       <  1:4874582/140‑1 (MQ=255)
                                                                                                    tCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTcaca                                       >  2:5877384/1‑140 (MQ=255)
                                                                                                       gCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATTATTTCTAAGCCTAGTTTAATCGAATAATTCATCACTGCAGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCAAGCACTTTTTCAGCCCCTTCCTTCACATCg                                    <  1:7435296/140‑1 (MQ=255)
                                                                                                       gCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCg                                    <  1:3807083/140‑1 (MQ=255)
                                                                                                       gCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCg                                    <  1:1089416/140‑1 (MQ=255)
                                                                                                       gCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCg                                    <  1:1304087/140‑1 (MQ=255)
                                                                                                       gCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCg                                    <  1:1801717/140‑1 (MQ=255)
                                                                                                       gCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCg                                    <  2:5600046/140‑1 (MQ=255)
                                                                                                       gCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCg                                    <  1:480687/140‑1 (MQ=255)
                                                                                                       gCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCg                                    <  2:2589045/140‑1 (MQ=255)
                                                                                                       gCGTTTCGAGCGGAACAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCg                                    <  1:6076044/140‑1 (MQ=255)
                                                                                                         gTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTa                                  <  1:2694474/140‑1 (MQ=255)
                                                                                                                   gATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCg                        <  2:5229983/140‑1 (MQ=255)
                                                                                                                   gATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCg                        <  2:6624769/140‑1 (MQ=255)
                                                                                                                    aTCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCgg                       >  2:3018693/1‑140 (MQ=255)
                                                                                                                      cAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTTATCGAATAATTCAGCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAg                     <  2:2692576/140‑1 (MQ=255)
                                                                                                                        aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca                   <  2:3698722/140‑1 (MQ=255)
                                                                                                                        aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca                   <  2:6833666/140‑1 (MQ=255)
                                                                                                                              ggAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTc             >  1:3151344/1‑140 (MQ=255)
                                                                                                                                  gaagaaTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCAc                                                             <  1:2245799/88‑1 (MQ=255)
                                                                                                                                  gaagaaTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCAc                                                             >  2:5562164/1‑88 (MQ=255)
                                                                                                                                   aagaaTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGc                                                                    >  2:6580720/1‑80 (MQ=255)
                                                                                                                                   aagaaTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCTGTAt        >  2:7759479/1‑140 (MQ=255)
                                                                                                                                   aagaaTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTAt        >  2:5939954/1‑140 (MQ=255)
                                                                                                                                   aagaaTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTAt        >  1:188580/1‑140 (MQ=255)
                                                                                                                                   aagaaTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTCCACATCGTACATCCAAGCGGAg                     >  1:2524680/1‑127 (MQ=255)
                                                                                                                                   aagaaTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCACAGCACTTTTTCAGCCCCTTCCTTCACATCGTAAATACAAGCGGAGAACATTTCAGTAt        >  2:740719/1‑140 (MQ=255)
                                                                                                                                       aTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTATTGGc    <  1:452635/140‑1 (MQ=255)
                                                                                                                                         cATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTATTGGCCa  <  2:5720109/140‑1 (MQ=255)
                                                                                                                                         cATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTATTGGCCa  <  2:191457/140‑1 (MQ=255)
                                                                                                                                         cATTTCGAAGCCTAGTTTAATCGAATAATTCATAATTGCCGTCAGTCGTTATACGTGAAAAAACGGGACGATGCCAAGAACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGTAGCACATTTCAGTATTGGCCa  <  1:5400465/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
GATGACAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTATTGGCCA  >  NZ_CP034943/2729795‑2730071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: