Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,729,937 C→A 6.1% E277* (GAA→TAA)  lcfA ← long‑chain‑fatty‑acid‑‑CoA ligase LcfA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,729,9370CA 6.1% 1285.6 / 13.3 471E277* (GAA→TAA) lcfAlong‑chain‑fatty‑acid‑‑CoA ligase LcfA
Reads supporting (aligned to +/- strand):  ref base C (81/323);  new base A (45/2);  total (146/325)
Fisher's exact test for biased strand distribution p-value = 1.01e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 471 total aligned reads displayed.

ACAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTATTGGCCAAAAT  >  NZ_CP034943/2729799‑2730075
                                                                                                                                          |                                                                                                                                          
aCAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCg                                                                                                                                           <  1:4905021/140‑1 (MQ=255)
aCAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCg                                                                                                                                           <  1:1537158/140‑1 (MQ=255)
aCAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCg                                                                                                                                           <  1:4312377/140‑1 (MQ=255)
aCAGATCATAATGCAGCAATTCGGGGTGCTGCAAAAGGCCAATGTTAATTGTCGGTGCAACCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATGTTCAGCGTTTCGAGCGGATCAAACTTAGGACGAAGAATCATTTCg                                                                                                                                           <  1:2716469/140‑1 (MQ=255)
    aTCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTTTTTGTCGATTATTTTACGCGTTTCGAGCGGATCAAACATAGGAAGAAGAATCAATTAGAAGc                                                                                                                                       >  2:4528099/1‑140 (MQ=255)
     tCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGcc                                                                                                                                      <  2:573606/140‑1 (MQ=255)
     tCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGcc                                                                                                                                      <  2:3885994/140‑1 (MQ=255)
           tgctgcAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTAAGCGTTTCGAGCCGCTCAAACTTATGAAGAAGAATCATTTGGAAGCCGAGgtt                                                                                                                                >  2:6158743/1‑140 (MQ=255)
              tgcAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGACAGAGAGTCGGTTTTTGTTTGTCGATGATTTTAAGCGTTTCGAGCGGATCAAAAGTAGGAAGAAGAATAATTTAGAAGCCGAGTTTAAt                                                                                                                             >  2:859135/1‑140 (MQ=255)
              tgcAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTTTAGATGATTTTGAGCGTTTGGAGCGGATAAAAAGTAGGAATAATAATCATTTATAATACTAGTTTAAt                                                                                                                             >  1:3296775/1‑140 (MQ=255)
              tgcAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTGAGCGTTTCGAGCGGCTCAAACATAGGAAGAAGAATCATTTCTAATCCGAGTTTAAt                                                                                                                             >  1:5717189/1‑140 (MQ=255)
              tgcAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTGTTTTTGGATTATTTTCAGCGGTTGGCGCGGGTCCAAAATAGGTAGAAAAATAATTTAGAAGCCGAGttttag                                                                                                                             >  1:5922484/1‑137 (MQ=255)
                cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTTTTTGTCGATGATTTTCAGCGTTTAGAGCGGATAAAAATTAGGAACAAGAAACAATTGAAAGCCTAGTTTaaaag                                                                                                                           >  2:4197294/1‑137 (MQ=255)
                cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTGGATGATTTTCAGCGTTTCGAGCGGAGAAAACATATGAAAAAGAATAATTTAGAATCCTAGTTTAAtag                                                                                                                           >  1:6439297/1‑138 (MQ=255)
                cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATTATTTTAAGCGGTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAAtag                                                                                                                           >  2:4029216/1‑138 (MQ=255)
                cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGTTTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTGGAAGCCTAGTTTAATCg                                                                                                                           >  2:4371174/1‑140 (MQ=255)
                cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTAAGCGTTTAGCGCGGATCAAACTTAGGAACAAGAATCATTTAGAAGCCGAGTTTAATCg                                                                                                                           >  1:5429905/1‑140 (MQ=255)
                cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTGTTTGTGTGTCGCTGATTTTCAGCGTTTCGAGCGGAGCAAAAATAGGAAGAAGAATAATTTAGAACGCGAGGTTAAtag                                                                                                                           >  1:2816732/1‑138 (MQ=255)
                  aTTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGaa                                                                                                                         <  2:32875/140‑1 (MQ=255)
                  aTTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGaa                                                                                                                         <  1:4487790/140‑1 (MQ=255)
                     cGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTTTCGATGATTTTAAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATAATTTCGAAGCCTAGTTTAAACGaataa                                                                                                                      >  2:5640769/1‑140 (MQ=255)
                       gggTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAAtt                                                                                                                    <  1:5825404/140‑1 (MQ=255)
                       gggTGATGCAAAAGGCCAATGTAAATGGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAAtt                                                                                                                    <  1:1674531/140‑1 (MQ=255)
                        ggTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGGTTTTCGATGTTTTTAAGCGTTTCGAGCGGATCAAAATTATGAAGAAGAATCATTTAGAACCCTAGTTTAATAGAAAAAtta                                                                                                                   >  2:5502632/1‑139 (MQ=255)
                        ggTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTGTTTGTCGATTGTTTTAACCGGGTGGAGCGGATCAAAAGTAAGAACAATATTAATTTAGACGCCTCGGTTAATAGCATAAtta                                                                                                                   >  1:675987/1‑139 (MQ=255)
                           gATGCATAAGTACTATGTAAATTGTCGGTGCAGCCGGAAATAGATTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGAGGATAAAACTTATGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATtcatc                                                                                                                <  2:6285113/140‑1 (MQ=255)
                           gATGCAACAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATtcatc                                                                                                                <  1:2048875/140‑1 (MQ=255)
                            aTGCAAAAGGCCAATGTACATTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTGTTTGTCGGAGATTTTAAGCGTTTCGAGCGGCTCAAACTTAGGAAGAACAAACAATTAGAAGCCGAGGTTAATCGAATAAAtcaaac                                                                                                               >  1:3210687/1‑137 (MQ=255)
                            aTGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATtcatca                                                                                                               <  2:186515/140‑1 (MQ=255)
                                  caGGCGACTGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTGGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCg                                                                                                         <  1:7327272/139‑1 (MQ=255)
                                  aaGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCg                                                                                                         <  1:1386358/140‑1 (MQ=255)
                                  aaGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAAACATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCg                                                                                                         <  1:4676791/140‑1 (MQ=255)
                                  aaGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGATTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGCATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCg                                                                                                         <  1:7372561/140‑1 (MQ=255)
                                   aGGCCAATGTAAATTGTCGGTGCACCCGTAAAGAGACTCGGTTTTTGGTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGt                                                                                                        <  2:7272973/140‑1 (MQ=255)
                                    ggCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCTGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTc                                                                                                       <  1:6910905/140‑1 (MQ=255)
                                       cAATGTAAATTGTCTGTGCACCCGGAAAGAGAGTCGGTTTTTGTTGGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGt                                                                                                    <  1:7550110/140‑1 (MQ=255)
                                       cAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGt                                                                                                    <  1:3080960/140‑1 (MQ=255)
                                       cAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGt                                                                                                    <  1:981363/140‑1 (MQ=255)
                                       cAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGt                                                                                                    <  1:6302654/140‑1 (MQ=255)
                                       cAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGt                                                                                                    <  1:7479911/140‑1 (MQ=255)
                                               aTTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTTTGAAGAAGAATCAATTCGAAGCCTAGTTTAATCGAATAATTCATAACTTCCGTCAGTCCGtgaaa                                                                                            >  2:7555078/1‑136 (MQ=255)
                                                  gTCGGTGCACCCGGAAAGAGAGTCGGTTTTTTTTTGTCGATTATTTTTAACGGTTAGAGCGGATAAAAAATATTAAAAAAAATAATTTATAAGCCAAGTTTAATAGAATAAATAAACACAGCCCGCACTCCCTATAcaat                                                                                         >  1:2517989/1‑137 (MQ=255)
                                                    cGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATTAATTTCATCGTTTAGAGCGGATCAAAATTATGAAGAAGAATAATTTCGAAGCCAAGTTTAAAAGAATAATTCAACAAGGCCGTCAGTCCGTATAAGTGaa                                                                                       >  2:5121458/1‑140 (MQ=255)
                                                    cGGTGCACCCGGAAAGAGAGTCGGTTTTTGGTTGTGGATTATTTTCAGCGATTCGAGCGGAGCAAAAAAAGGAAGAACACTCAATTCCAAGCCTAGTTTAATAGAATAATTCATCAATGCCCTCAGTCCGTATACCTGaa                                                                                       >  2:3764581/1‑140 (MQ=255)
                                                               gAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACg                                                                            <  1:6693516/140‑1 (MQ=255)
                                                                  agagagTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATg                                                                         <  2:2994773/140‑1 (MQ=255)
                                                                    agagTCGGTTTTTTTTTGTCGATGATTTTCACCGTTTCGAGCGGATCAAAAATAGGAAAAACAATCATTTCGAATCCTAGTTTAATCGAATAAATCATAACTGCCGTCAGTCCCTATAAGTTAAAAAAAGGGACGATTcc                                                                       >  2:1521192/1‑140 (MQ=255)
                                                                    agagTCGGGTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTATGAACAACAAAAATTTCGAAGCCTAGTTTAATCGAAAAATTCATCACAGCCGGCAGTCCGTATAAGTGAAAAAAAGGGACGATGcc                                                                       >  2:6971847/1‑140 (MQ=255)
                                                                     gagTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGccc                                                                      <  1:6576145/140‑1 (MQ=255)
                                                                        tCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGc                                                                   <  2:1187559/140‑1 (MQ=255)
                                                                         cGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCa                                                                  <  1:3744474/140‑1 (MQ=255)
                                                                          ggTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCAc                                                                 <  2:3226440/140‑1 (MQ=255)
                                                                               ttgtttgtCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACttttt                                                            <  1:3515119/140‑1 (MQ=255)
                                                                                    tgtCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcc                                                       <  1:855553/140‑1 (MQ=255)
                                                                                    tgtCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcc                                                       <  2:5883585/140‑1 (MQ=255)
                                                                                    tgtCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcc                                                       <  2:5438429/140‑1 (MQ=255)
                                                                                    tgtCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcc                                                       <  2:246485/140‑1 (MQ=255)
                                                                                      tCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGTCCAGCACTTTTTCAGcccc                                                     <  1:4821610/140‑1 (MQ=255)
                                                                                      tCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcccc                                                     <  1:1483135/140‑1 (MQ=255)
                                                                                      tCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcccc                                                     <  1:6083421/140‑1 (MQ=255)
                                                                                      tCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcccc                                                     <  2:6653608/140‑1 (MQ=255)
                                                                                         atgatTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCccttc                                                  <  1:4386820/140‑1 (MQ=255)
                                                                                         atgatTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCccttc                                                  <  1:5360057/140‑1 (MQ=255)
                                                                                            atTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCccttcctt                                               <  1:100808/140‑1 (MQ=255)
                                                                                              tttCATCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTca                                             <  2:6053942/140‑1 (MQ=255)
                                                                                              tttCAGCGTTTCGTTCGGATCAAAATTAGGATGATTAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCTTAAGTCCGTATACTTGAAAAAACGGGACGATGCCCAGAACTTTTTCAGCCCCTTCCTTca                                             <  2:7080928/140‑1 (MQ=255)
                                                                                              tttCAGCGTTTCGAGCGTATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCTAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTca                                             <  2:7250741/140‑1 (MQ=255)
                                                                                              tttCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTca                                             <  1:4051989/140‑1 (MQ=255)
                                                                                              tttCAGAGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATACATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTca                                             <  1:3637859/140‑1 (MQ=255)
                                                                                                tCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTcaca                                           <  2:6585947/140‑1 (MQ=255)
                                                                                                   gCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCg                                        <  1:1089416/140‑1 (MQ=255)
                                                                                                   gCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCg                                        <  1:5153289/140‑1 (MQ=255)
                                                                                                   gCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCg                                        <  2:2841130/140‑1 (MQ=255)
                                                                                                     gTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTa                                      <  1:5546543/140‑1 (MQ=255)
                                                                                                          gAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATcc                                 <  2:5577919/140‑1 (MQ=255)
                                                                                                               gATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCATCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCg                            <  1:7190810/140‑1 (MQ=255)
                                                                                                                aTCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCgg                           >  2:4228552/1‑140 (MQ=255)
                                                                                                                aTCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCgg                           >  1:3846724/1‑140 (MQ=255)
                                                                                                                    aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca                       <  2:3417627/140‑1 (MQ=255)
                                                                                                                    aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca                       <  2:814354/140‑1 (MQ=255)
                                                                                                                    aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca                       <  2:6833666/140‑1 (MQ=255)
                                                                                                                    aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca                       <  1:173287/140‑1 (MQ=255)
                                                                                                                    aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca                       <  1:2217790/140‑1 (MQ=255)
                                                                                                                    aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca                       <  2:3624715/140‑1 (MQ=255)
                                                                                                                    aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca                       <  1:5260518/140‑1 (MQ=255)
                                                                                                                    aaCTTAGGAAGAAGAATCAGTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATCCGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca                       <  1:7692566/140‑1 (MQ=255)
                                                                                                                          ggAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTc                 >  1:2432281/1‑140 (MQ=255)
                                                                                                                              gaagaaTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCAc                                                                 >  2:2245799/1‑88 (MQ=255)
                                                                                                                               aagaaTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAg                         <  2:2628309/127‑1 (MQ=255)
                                                                                                                               aagaaTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTAt            >  1:5110845/1‑140 (MQ=255)
                                                                                                                               aagaaTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTAt            >  1:188580/1‑140 (MQ=255)
                                                                                                                               aagaaTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCACAGCACTTTTTCAGCCCCTTCCTTCACATCGTAAATACAAGCGGAGAACATTTCAGTAt            >  2:740719/1‑140 (MQ=255)
                                                                                                                               aagaaTAATTTCGAAGCCTAGTTTAATATAAAAATTCATAAATTCAGTCATACATTATAAGTGAAAAAACTGTACTATGACCATCACTTTTTCAGCCACTTCATTCACATCGTACATCCAAGCTGAGCACATTTAAGTAt            >  2:5260518/1‑140 (MQ=255)
                                                                                                                                agaaTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTAtt           >  2:1659795/1‑140 (MQ=255)
                                                                                                                                agaaTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATGTCAGTAtt           >  2:7647070/1‑140 (MQ=255)
                                                                                                                                 gaaTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTATTg          >  2:4802269/1‑140 (MQ=255)
                                                                                                                                     cATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTATTGGCCa      <  1:6846762/140‑1 (MQ=255)
                                                                                                                                     cATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTATTGGCCa      <  1:4542854/140‑1 (MQ=255)
                                                                                                                                       tttCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTATTGGCCaaa    <  1:1263521/140‑1 (MQ=255)
                                                                                                                                         tCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTATTGGCCAAAAt  >  1:2663104/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
ACAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTATTGGCCAAAAT  >  NZ_CP034943/2729799‑2730075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: