Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,812,790 T→G 8.6% E199D (GAA→GAC EO946_RS14520 ← SDR family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,812,7900TG 8.6% 626.1 / 13.8 326E199D (GAA→GACEO946_RS14520SDR family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (171/112);  new base G (40/2);  total (212/114)
Fisher's exact test for biased strand distribution p-value = 1.37e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 330 total aligned reads displayed.

GACCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCAGCGCCAAATGCC  >  NZ_CP034943/2812651‑2812928
                                                                                                                                           |                                                                                                                                          
gaCCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCt                                                                                                                                            <  2:4322241/140‑1 (MQ=255)
  ccTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTc                                                                                                                                          <  1:7709246/140‑1 (MQ=255)
  ccTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTc                                                                                                                                          <  1:684994/140‑1 (MQ=255)
  ccTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTc                                                                                                                                          <  2:329297/140‑1 (MQ=255)
    tCCGGTGGCTTCAAAGACAGTTAATTTAACAAGCACTTAGTTAACCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTAGCGTACGGGCttacg                                                                                                                                        >  2:3498424/1‑137 (MQ=255)
     ccGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCCGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTGCCTGGCGTCctc                                                                                                                                       >  2:2231677/1‑140 (MQ=255)
     ccGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCCCTTGGCTTACTGGCTTGctc                                                                                                                                       >  1:4781634/1‑140 (MQ=255)
     ccGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCctc                                                                                                                                       >  2:6072467/1‑140 (MQ=255)
     ccGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCctc                                                                                                                                       >  1:5068683/1‑140 (MQ=255)
     ccGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCctc                                                                                                                                       >  1:3649644/1‑140 (MQ=255)
     ccGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCGTActc                                                                                                                                       >  2:3199979/1‑140 (MQ=255)
     ccGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCGTCCTGGCGTccgc                                                                                                                                       >  1:1236192/1‑138 (MQ=255)
     ccGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTACTTGCCGATTCGCTTCCTGGCTTGctc                                                                                                                                       >  1:4865718/1‑140 (MQ=255)
     ccGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTCCTTGCCGATTCGCTTCCTGGCTTCctc                                                                                                                                       >  2:4822215/1‑140 (MQ=255)
      cGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCGTCCTATCGGCTTTTTTTCCGTGACGATTCGCTTCCTGGGTTCctcg                                                                                                                                      >  2:528782/1‑139 (MQ=255)
        gTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTAt                                                                                                                                    <  2:1212485/140‑1 (MQ=255)
           gCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGCTTCGCTTACTGGCTTCCTCTATTGa                                                                                                                                 >  2:2447727/1‑140 (MQ=255)
             ttCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCGTGCCGCTTCGCTTCCTGGCTTCCTCGATTgttt                                                                                                                               >  2:6569475/1‑137 (MQ=255)
             ttCAATGACAGTGCCGGTCACAAGGTCTGAGTTCTCCGCACACTAGAAAGCGCTAATCCGGGCGATATCCTCGCCTGTGCCAGAGCTTCCTATCGGCGTTTTTTCCTTTCCGATGCGCTTCATGGCTTCCGGGATTGATg                                                                                                                               >  2:5754876/1‑140 (MQ=255)
              tCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCGTCCTGGCTTCCGCTAGTGATGc                                                                                                                              >  1:5646825/1‑140 (MQ=255)
                       gTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGGTTCCGCTATTGATGcgtccttcct                                                                                                                     >  1:6176656/1‑138 (MQ=255)
                       gTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTGCTTTCCGATTCGCGTCCTGGCTTCCTCTAGTGATGcttcctggat                                                                                                                     >  1:1043724/1‑136 (MQ=255)
                       gTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTCCTGGCCGATTCGCATCCTGTCTTCCTCTATTGATGCTTCCGTCAt                                                                                                                     >  1:1738388/1‑140 (MQ=255)
                       gTGCCTGTCACAAGGACTGAGATCTCCTCACACAATAAAGCCATAAACCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGAGTTTTTTCCTGGCCCATTCGCTTGCTGGCTTCCTCTCTTGGTGCGTCCGTCAt                                                                                                                     >  2:7143604/1‑140 (MQ=255)
                         gCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCGATTCGCTTCCTGGCTTCCTCTATTCATGCTTCCTTCATGt                                                                                                                   <  2:6242284/140‑1 (MQ=255)
                          ccTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGCTCTTCCTATCGGCGTTTTTTCCTTTCCGATTCGCTTCCTGGCTTCCTCTATTGTTTGTTCCTTCATGTc                                                                                                                  >  2:7664417/1‑140 (MQ=255)
                          ccTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTCCTTGCCGATTCGCTTCCTGGCTTCCGCGATTGATGCTTGCGTCATGTc                                                                                                                  >  1:4473019/1‑140 (MQ=255)
                              tCACAAGGTCTTAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTGTTCCTTTCAGATTAGCTTCCAGGCTTACTATATTTATTATTCCTTCATGTCGCCg                                                                                                              >  1:1821959/1‑140 (MQ=255)
                              tCACAAGGTCTGAGTTCTCCTCACACAAGAACGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTGTTGCTTGCCGCTTCGCGGCCGGGCTTCCTCTTTTGATGCGTGCGTCATGTCGGCg                                                                                                              >  1:6400421/1‑140 (MQ=255)
                              tCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTGCTTCATGTCGCCg                                                                                                              >  1:7040509/1‑140 (MQ=255)
                               cacaAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATACTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGa                                                                                                             <  2:4672907/140‑1 (MQ=255)
                               cacaAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATACTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGa                                                                                                             <  2:2879834/140‑1 (MQ=255)
                               cacaAGGTCTGAGTTCTCCGAACACCAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGa                                                                                                             <  2:5403884/140‑1 (MQ=255)
                                  aaGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCCATTGGCGTACTGGGTGCCGCTAGTGTTTGTTCGGAGAGGTCGCCGACaa                                                                                                          >  1:594994/1‑140 (MQ=255)
                                  aaGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTACTGGCTTCCTCTATTGATGCTTACTTCATGTAGCCCAcca                                                                                                          >  1:239142/1‑138 (MQ=255)
                                   aGGTCTGAGTTCTCCTCACACCAGAAAGCGATACTCCGGGCGATATCCTCGCCTGTGCCAGATCGTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAAt                                                                                                         <  1:6973085/140‑1 (MQ=255)
                                   aGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAAt                                                                                                         <  1:6876685/140‑1 (MQ=255)
                                   aGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAAt                                                                                                         <  2:5854929/140‑1 (MQ=255)
                                   aGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAAt                                                                                                         <  1:911652/140‑1 (MQ=255)
                                    ggTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTGCTGGCGTCCTCTATTGATGCTTCCTTAATTTCGCCGACCAtt                                                                                                        >  2:6427005/1‑139 (MQ=255)
                                    ggTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATg                                                                                                        >  2:3698069/1‑140 (MQ=255)
                                    ggTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCGTTCCGATTCGCTTCCTGGCGTGCGCTATTGATGCTTCCTTCCTTTCGCCGACAATg                                                                                                        >  1:5134204/1‑140 (MQ=255)
                                        tGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTTCCGATTAGCTTACTTGGTTGCTCTATTGATGCTTCCATCAAGTCGCCGACAAtttaac                                                                                                    >  1:4321362/1‑135 (MQ=255)
                                             tctcCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCggg                                                                                               <  1:3942962/140‑1 (MQ=255)
                                               tcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCGATTCGCGTACTGGCGTCCTCTATTGATGCTTCCTTCATGTCGCCGACAAAGTCACCCCGGCa                                                                                             >  1:6746247/1‑140 (MQ=255)
                                               tcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATTCTTCCTTCATGTCGCCGACAATGTCACCCGGGCa                                                                                             >  2:4498900/1‑140 (MQ=255)
                                               tcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTGTCTGGCTTCCTCTAATGAAGCTTCCTTGAATGCGCCGAAAATTTCACCCGGGCa                                                                                             >  1:7413798/1‑140 (MQ=255)
                                               tcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCGTCCTTGCTTCCGCTATTTATTCTTCCATCATGTGGCCCACCAAGTCACCCCGGcc                                                                                             >  1:1170303/1‑139 (MQ=255)
                                               tcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCGTGCCGATTCGCGTACTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAAAGTCACCCGGGCa                                                                                             >  1:5906666/1‑140 (MQ=255)
                                               tcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTACTTGCCGATTCGCTTCCTGGCTTCCGCTATTGATGCTTCCTTCAATTAGCCGACAATGTCACCCCGGCa                                                                                             >  1:3567279/1‑140 (MQ=255)
                                                cctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCaa                                                                                            >  2:533639/1‑140 (MQ=255)
                                                  tcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAAc                                                                                          <  1:1938052/140‑1 (MQ=255)
                                                    acacaAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCGATTCGTGTCCTGGCTTCCTCTATTGATGCTTCCTTCAAGTCGCCGACAATGTCACCCCGGCAAACCa                                                                                        >  1:4981070/1‑140 (MQ=255)
                                                    acacaAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCGTCCTCTATTGATGGTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAAccc                                                                                        >  2:352178/1‑139 (MQ=255)
                                                        aaGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGtt                                                                                    <  2:4976661/140‑1 (MQ=255)
                                                         aGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGAACATGTCACCCGGGCAAACCATGTTg                                                                                   >  2:3837461/1‑140 (MQ=255)
                                                           aaaGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCCTGTCGCCGACAATGTCACCCGGGCAAAACATGTTGGc                                                                                 >  2:1166249/1‑140 (MQ=255)
                                                                gATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTGCTTGGCGATTCGCGTGCTGGGTTGCGCGATTGTTGCTTCCTTCATGTCGGCGCCAATGTCACCCGGGCCCACGATGTTgggcgttt                                                                            >  1:374255/1‑134 (MQ=255)
                                                                         ggCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCGATTCGCTTCCTGGGTTGCGCTATTGTTGCTTACTTCAATTGGCCGCCAAAGTCACCCGGGCAAACCAAGTTGGCAGTTATTCCGAAtt                                                                   >  1:819569/1‑140 (MQ=255)
                                                                         ggCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCCAATCTCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAAAGTCACCCCGGCCAACCATGTTGGCAGGTAGGCCGAAtt                                                                   >  2:3580247/1‑140 (MQ=255)
                                                                         ggCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCATTGCCGAATAGCGTCCAGGCTTCCGCTAATGATTCTTACATAATGTCGCAGACAAAGTCACCCGGGCAAACCATGTTAGAAGTTATGCCCAAtt                                                                   >  2:1943235/1‑140 (MQ=255)
                                                                         ggCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTGCATTGCGAATCGCTTGCTTGCTTACTGTATTGATTCTTCCGTCATTTCGCCGCCAAAGTCACCCCGGCAAACCAAGTTTGGAGTTATGCCGAAtt                                                                   >  1:977607/1‑140 (MQ=255)
                                                                         ggCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTCCTTGCCGATTCGCTTCCTGGGTTGCTCTATTGATGCTTCCGTCAAGTCGCCGACAAAGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAAtt                                                                   >  1:3515482/1‑140 (MQ=255)
                                                                            gATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCGTCCATGCGTCCGCTATTGATTCCGCCTTCATTTCGCCGACAATGTCACCCCGGCACACCATGGTTGCCGTTATGGCGAATTAgg                                                                >  1:3448583/1‑140 (MQ=255)
                                                                            gATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTGCTTGCCGATTCGCTTGCTGGCGTGCTCTATTGATGGGTCCTTGATGGCGCCGACAAAGTCACCCGGGCAAACCAAGTTGGGAGGTATTCCGAATTCgg                                                                >  1:407797/1‑140 (MQ=255)
                                                                             ctatCCTCGCCTGTGCCAGATCGTCCTATCGGCGTTTTGTCCGTGCCGATGCGCTTCCTGGCTTCCTCTAGTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATCCCGAATTCGGc                                                               <  2:2806978/139‑1 (MQ=255)
                                                                             atatCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGc                                                               <  2:7388297/140‑1 (MQ=255)
                                                                             atatCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGc                                                               <  2:7421898/140‑1 (MQ=255)
                                                                             atatCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGc                                                               <  1:6569475/140‑1 (MQ=255)
                                                                              tatCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTAGCTTCCTGGCTTCCGCTATTGATGCTTCCGTCATGTCGCCGTCCAGGTCACCCGGGCAAACCCTGTTGGGAGTTATGGCGAATTCGGct                                                              >  2:3935959/1‑140 (MQ=255)
                                                                                    cGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTTCCGATTCGCTTCCTGGCGTCCTCTATTGATGCTTCCTTCATGTCGCCGACAAGGTCACCCGGGCAAACCAAGGTGGCAGTTATGCCCAATTCGGCTTCTTca                                                        >  2:1659708/1‑140 (MQ=255)
                                                                                    cGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTTCCGATTCCCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCCACAAAGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAAATCGGCTTCTTca                                                        >  2:5091350/1‑140 (MQ=255)
                                                                                    cGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTCTTGATGCGTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTca                                                        <  2:2831210/140‑1 (MQ=255)
                                                                                    cGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTca                                                        <  2:4635072/140‑1 (MQ=255)
                                                                                    cGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCAACCGGGCAAACCAAGTTGGCAGTTATTCCGAAATCGGcttattaa                                                        >  2:4014800/1‑138 (MQ=255)
                                                                                    cGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCATGCCGATTCGCTACCTGGCTTCCTATATTGATTCTTCCATCAAGTCCCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATTCCCAATTCGGCttcttcc                                                        >  2:1381363/1‑139 (MQ=255)
                                                                                     gCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTcaa                                                       <  1:4649628/140‑1 (MQ=255)
                                                                                     gCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTcaa                                                       <  1:2663613/140‑1 (MQ=255)
                                                                                          tgCCAGATCTTCCTATCGGCGTTTTTTTCTTGCCGTTTAGCTTCCTGGCTTCGTCGATTGATTCGTCCGTCCTGTGGCCGACCATGTCCCCCGGGGAAACCCTGTTGGCGGTTATTCCGAATTCGGCGTGTTcaaaggca                                                  >  1:5472112/1‑140 (MQ=255)
                                                                                          tgCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTGCTCTATTGCTGCTTCCGTCATGTCGCCGACAATGGCACCCGGGCCACCCATGTTGGCCGGGATGCCGAATTCGGCTTCGTcaatggcc                                                  >  2:1481091/1‑139 (MQ=255)
                                                                                          tgCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCGTCCTGGCTTCCTCTATTTTTGGTTCCTTCATGTGGCCCACAATGTGACCCCGGCAAACCAGGTTGGCAGTTATTGCGAAATCGGCTTCTTaaaatgca                                                  >  1:5339630/1‑135 (MQ=255)
                                                                                          tgCCAGATCTTCCTATCGGCGTTTTTTACTTGCCGATTCGCTTCCTGGGTTCCTCTATTGATGCTTCCTTCATGTCGCCCACAATGTCACCCGGGCAAACCATTTTGGCAGTTATGCCGAATTCGGCGTCTTcaatggca                                                  >  2:1883262/1‑140 (MQ=255)
                                                                                          tgCCAGATCTTCCTATCGGCGTTGTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCGTCCGTCATGTCGCCCACAATGTCACCCCGGCAAACCAAGTTGGCAGTTATGCCGAATTCGGCTTCGTaaatggca                                                  >  2:4248866/1‑140 (MQ=255)
                                                                                           gCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTcaatggcaa                                                 <  2:6489328/140‑1 (MQ=255)
                                                                                           gCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTcaatggcaa                                                 <  1:1473797/140‑1 (MQ=255)
                                                                                           gCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTcaatggcaa                                                 <  1:3203193/140‑1 (MQ=255)
                                                                                           gCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTcaatggcaa                                                 <  2:2657676/140‑1 (MQ=255)
                                                                                               gATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGt                                             <  2:7002434/140‑1 (MQ=255)
                                                                                                    tCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCg                                        <  2:6652407/140‑1 (MQ=255)
                                                                                                     ccTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCGTCCTCGATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCCTGTTGGCAGTTATTCCCAATTCGGGGTCATAAATTGCAAATGTCTTAGt                                       >  2:6912237/1‑140 (MQ=255)
                                                                                                        aTCGGCGTTTTTTCCTTGCCGATTCCCTTCCAGGCTTCCTCTAATGATGCTTCCTTCATGTCGCCCACAATGTCACCCCGGCAAACCATGTTGGCAGTTATGCCGAAATCGGCTTCTTCCATGGCAATGGTCTTCGTCaa                                    >  2:7094263/1‑140 (MQ=255)
                                                                                                         tCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCaaa                                   <  1:2605747/140‑1 (MQ=255)
                                                                                                               ttttttCCTTGCCGATTCGCTTCCTGGCTTCCTCTAGTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGcc                             <  1:2081371/140‑1 (MQ=255)
                                                                                                                    tCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAAcc                        <  1:2861892/140‑1 (MQ=255)
                                                                                                                              ttCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAACGAAGCCAAACCCACTTTCGCa              <  1:1607366/140‑1 (MQ=255)
                                                                                                                              ttCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCa              <  1:214824/140‑1 (MQ=255)
                                                                                                                                  cTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCAgcgc          >  2:2952764/1‑140 (MQ=255)
                                                                                                                                   ttCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCAGCGcc         <  1:3109282/140‑1 (MQ=255)
                                                                                                                                   ttCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCAGCGcc         <  1:6646621/140‑1 (MQ=255)
                                                                                                                                   ttCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCAGCGcc         <  1:7131083/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GACCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCAGCGCCAAATGCC  >  NZ_CP034943/2812651‑2812928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: