Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,851,294 G→T 6.2% P60T (CCC→ACC)  murC ← UDP‑N‑acetylmuramate‑‑L‑alanine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,851,2940GT 6.2% 1175.2 / 17.0 418P60T (CCC→ACC) murCUDP‑N‑acetylmuramate‑‑L‑alanine ligase
Reads supporting (aligned to +/- strand):  ref base G (134/242);  new base T (2/39);  total (136/282)
Fisher's exact test for biased strand distribution p-value = 1.38e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 430 total aligned reads displayed.

ACGGCAACACTAGTGAATTTTTTCATGTAGTCGCCTAAAAACTTATGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCATGAAGTATTTGGGCAAGCGGACTCATA  >  NZ_CP034943/2851155‑2851433
                                                                                                                                           |                                                                                                                                           
aCGGCAACACTAGTGAATTTTTTCATGTAGTCGCCTAAAAACTTATGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCggg                                                                                                                                             >  1:3855986/1‑140 (MQ=255)
 cGGCAACACTAGTGTATTTTTTCATGTAGTCGCCTAAAAACTTTTGAGAACGTATCACCGGAATTCCGTCAGACATGGCTTTGTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGt                                                                                                                                            <  1:3655475/140‑1 (MQ=255)
 cGGCAACACTAGTGAATTTTTTCATGTAGTCGCCTAATAACTTATGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGt                                                                                                                                            <  1:7376152/140‑1 (MQ=255)
 cGGCAACACTAGTGAATTTTTTCATGTAGTCGCCTAAAAACTTCTGATACCGTATCACCGGAATTCCGTCAGACAGGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGt                                                                                                                                            <  1:5820752/140‑1 (MQ=255)
 cGGCAACACTAGTGAATTTTTTCATGTAGTCGCCTAAAAACTTATGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGt                                                                                                                                            <  1:7069755/140‑1 (MQ=255)
 cGGCAACACTAGTGAATTTTTTCATGTAGTCGCCTAAAAACTTATGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGt                                                                                                                                            <  1:2291985/140‑1 (MQ=255)
 cGGCAACACTAGTGAATTTTTTCATGTAGTCGCCTAAAAACTTATGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGt                                                                                                                                            <  2:670089/140‑1 (MQ=255)
 cGGCAACACTAGTGAATTTTTTCATGTAGTCGCCTAAAAACTTATGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGt                                                                                                                                            <  2:6112265/140‑1 (MQ=255)
 cGGCAACACTAGTGAATTTTTTCATGTAGTCGCCTAAAAACTTATGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGt                                                                                                                                            <  1:2687226/140‑1 (MQ=255)
 cGGCAACACTAGTGAATTTTTTCATGTAGTCGCCTAAAAACTTATGAGAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGt                                                                                                                                            <  2:4804867/140‑1 (MQ=255)
  ggCAACACTAGTGAATTTTTTCATGTAGTCGCCTAAAAACTTATGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGtt                                                                                                                                           <  1:5560485/140‑1 (MQ=255)
  ggCAACACTAGTGAATTTTTTCATGTAGTCGCCTAAAAACTTATGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGtt                                                                                                                                           <  1:3462102/140‑1 (MQ=255)
       cacTAGTGAATTTTTTCATGTAGTCGCCTAAAAACTTATGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGata                                                                                                                                      >  2:1030230/1‑140 (MQ=255)
            gTGAATTTTTTCATGTAGTCGCCTAAAAACTTATGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATc                                                                                                                                 >  2:2042761/1‑140 (MQ=255)
            gTGAATTTTTTCATGTAGTCGCCTAAAAACTTATGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATc                                                                                                                                 >  2:432285/1‑140 (MQ=255)
            gTGAATTTTTTCATGTAGTCGCCTAAAAACTTATGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATc                                                                                                                                 <  1:2552055/140‑1 (MQ=255)
            gTGAATTTTTTCATGTAGTCGCCTAAAAACTTATGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATc                                                                                                                                 <  1:1901572/140‑1 (MQ=255)
              gAATTTTTTCATGTAGTCGCCTAAAAACTTATGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCcg                                                                                                                               <  2:1430075/140‑1 (MQ=255)
                     ttCATGTAGTCGCCTAAAAACTTATGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTaaa                                                                                                                        <  2:3010157/140‑1 (MQ=255)
                                     acaaCTTATGATAACGTATCACCGGAATTCCGTCAGACCGGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAata                                                                                                        >  1:624376/3‑140 (MQ=255)
                                           tATGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCtt                                                                                                  <  1:7200944/140‑1 (MQ=255)
                                            aTGATAACTAATAACCCGAATTCAGTCAGACATGGATTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGAATGTCATGACGGGTTTGATATTATCATAGCTAAAAGGAAGAATCTTAATATTTCttt                                                                                                 >  2:5150841/1‑140 (MQ=255)
                                            aTGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGGGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAATGGAAGAATCGTAATATTTCttt                                                                                                 >  2:4100880/1‑140 (MQ=255)
                                            aTGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAAATTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCttt                                                                                                 >  2:4030951/1‑140 (MQ=255)
                                                   cGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAg                                                                                          >  1:7704459/1‑140 (MQ=255)
                                                   cGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAg                                                                                          >  2:4550460/1‑140 (MQ=255)
                                                       tCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCt                                                                                      >  2:879745/1‑140 (MQ=255)
                                                       tCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCt                                                                                      >  2:7097725/1‑140 (MQ=255)
                                                              aaTTCCGTCAGACATTGCTTTTTCAATTTCAGGATGCGTGTCTGTGAATGCGTTTCCGGCGATGACTGTCATGGCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCg                                                                               <  2:6465756/140‑1 (MQ=255)
                                                                           aTGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAAttt                                                                  >  2:4690322/1‑140 (MQ=255)
                                                                           aTGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAAttt                                                                  >  2:5896452/1‑140 (MQ=255)
                                                                             ggCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAAttttt                                                                >  1:475465/1‑140 (MQ=255)
                                                                             ggCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCGGTTTGCGTAAAAATAAAttttt                                                                >  2:635906/1‑140 (MQ=255)
                                                                             ggCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTATTTCAAGCGCTGTTTGCGTAAAAATAAAttttt                                                                >  1:2161799/1‑140 (MQ=255)
                                                                               cTTTTTCAATTTCAGGATTCGTGTCTGGGAATGCGTTTCCGGCGATGACTTTCATGGCGGTTTTTATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCg                                                              <  2:1582025/140‑1 (MQ=255)
                                                                                  tttCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGata                                                           >  1:7298589/1‑140 (MQ=255)
                                                                                   ttCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTAGatat                                                          >  2:2271816/1‑140 (MQ=255)
                                                                                   ttCAATTTCAGGATGCGTGTCTGGGAATGCGTTACCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGatat                                                          >  2:5719512/1‑140 (MQ=255)
                                                                                     cAATTTCAGGATGCGTTTCTGGGAATGCGTTTCCGGCGATGACTTTCATGCCTTGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAATTTTTTCGATATcc                                                        <  2:1651362/140‑1 (MQ=255)
                                                                                     cAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATcc                                                        <  1:3882005/140‑1 (MQ=255)
                                                                                     cAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATcc                                                        <  1:2042761/140‑1 (MQ=255)
                                                                                     aattttCAGGATGCTTGTCTGTGATTGAGTTTGCGTCGTGTACTGTCCTGCCTGTTTTGATATTATACGCGCTAAAAGGAAGAATCTTAATATTTCTTTTTTCAAGCGGTGTTTTCGTAAAAATAAATTTTTCGATATcc                                                        <  2:243132/137‑1 (MQ=255)
                                                                                     aaaTTTCAGGATGCGTTTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGTTTTGATATTATCCGCGCTAATAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATcc                                                        <  2:7567969/139‑1 (MQ=255)
                                                                                        tttCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGAt                                                     >  1:861793/1‑140 (MQ=255)
                                                                                        tttCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGAt                                                     >  2:3636623/1‑140 (MQ=255)
                                                                                        tttCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGAt                                                     <  1:6014719/140‑1 (MQ=255)
                                                                                          tctcgATGCTTGTCTGTGAATGCGTTTCCTTCGTTTATTGTCATGGCGTTTTTTATATTATCCGCGTTATTAGGAAGATTCGTAATATTTCTTTTTTCAAGCGCTGTTTGCTTTAATATAAATTTTTCGATATCCGATcc                                                   <  2:3511445/136‑1 (MQ=255)
                                                                                              gATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTGATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTgg                                               <  1:2873658/140‑1 (MQ=255)
                                                                                              gATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTgg                                               <  1:1698227/140‑1 (MQ=255)
                                                                                              gATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTgg                                               <  1:3124429/140‑1 (MQ=255)
                                                                                               tttcGTGTATGGTAATTCGTTTCCTGCGATGACTGTCTTGCCTGTTTTTATATTATCCGCTCTAAAAGTAAGAATCGTTATATTTCTTTTTTGAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  2:5354837/137‑1 (MQ=255)
                                                                                               aTGCGTGTCTGTGTATGCTTTTCCTGAGATGTCTGTCATGCCGTGTTTTATATTATCCGCTCTAAAAGGAAGAATCGTAATATTTGTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  2:25663/140‑1 (MQ=255)
                                                                                               aTGCGTGTCTGTGAATGCGTTTCCTGCGATGACTGTAATGCCTGGTTTGATATTATCCGCGATAAAAGGAAGAATCTTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCAGATCCTTGGa                                              <  1:432271/140‑1 (MQ=255)
                                                                                               aTGCGTGTCTGTGAATGCGTTTCCGGCTATGACTGTCATGCCGTGTTTGATATTATCCGCGCTAAAAGGAAGTATCGTTATATTTCTTTTTTAAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  2:3235966/140‑1 (MQ=255)
                                                                                               aTGCGTGTCTGGGATTGCGTTTCCGGCTATGACTTTCATGCCGTGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  2:4169652/140‑1 (MQ=255)
                                                                                               aTGCGTGTCTGGGAATGCGTTTCCGTAGTTGACTGTCATGCCGGGTTTGATATTATCCGCGCTAATAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  2:5058221/140‑1 (MQ=255)
                                                                                               aTGCGTGTCTGGGAATGCGTTTCCGGCTATGACTGTCATGCCGGTTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  2:5438132/140‑1 (MQ=255)
                                                                                               aTGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  2:7420311/140‑1 (MQ=255)
                                                                                               aTGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  2:7704459/140‑1 (MQ=255)
                                                                                               aTGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  2:475465/140‑1 (MQ=255)
                                                                                               aTGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  1:1070352/140‑1 (MQ=255)
                                                                                               aTGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  1:1191370/140‑1 (MQ=255)
                                                                                               aTGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  1:1451274/140‑1 (MQ=255)
                                                                                               aTGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  1:2450130/140‑1 (MQ=255)
                                                                                               aTGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  1:293625/140‑1 (MQ=255)
                                                                                               aTGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  1:3739125/140‑1 (MQ=255)
                                                                                               aTGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  1:4191765/140‑1 (MQ=255)
                                                                                               aTGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  1:7506854/140‑1 (MQ=255)
                                                                                               aTGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  1:4661771/140‑1 (MQ=255)
                                                                                               aTGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  1:480785/140‑1 (MQ=255)
                                                                                               aTGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  1:5150841/140‑1 (MQ=255)
                                                                                               aTGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGCCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGa                                              <  1:5410906/140‑1 (MQ=255)
                                                                                                   gtgtCTGGGAAAGCGTTTCCCGCGTTGACTGTCTTTCCGGTTTTGATATTCTCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCATATCCTTGGACatt                                          <  2:2169266/140‑3 (MQ=255)
                                                                                                     gtCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGtat                                        <  1:1030230/140‑1 (MQ=255)
                                                                                                          ggAATGCGTTTCCGGTTTTGTATGTCATGCCTGTTTTTTTTTTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCa                                   <  2:1289700/140‑1 (MQ=255)
                                                                                                          ggAATGCGTTTCCGGCGATGCCTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCa                                   <  1:566896/140‑1 (MQ=255)
                                                                                                            attGCGTTTCCGGCGTTTACTTTCATGCGGGTTTTGTTATTATCCTCTCTAAAAGGAAGAATCTTAATATTTCTTTTTTCTAGCGCTGTTTGAGTAAAAATAAATTTTTCGATATCCGATCCTTTGACAGTATATCCAtt                                 <  2:2325238/138‑1 (MQ=255)
                                                                                                            aaTGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCAtt                                 <  2:6288295/140‑1 (MQ=255)
                                                                                                            aaTGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCAtt                                 >  2:5598097/1‑140 (MQ=255)
                                                                                                                cGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCcg                                                                                                                               >  1:152618/1‑42 (MQ=255)
                                                                                                                cGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAATCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCa                             >  2:3809162/1‑140 (MQ=255)
                                                                                                                cGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCa                             >  2:2109614/1‑140 (MQ=255)
                                                                                                                  tttCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCATg                           <  1:6722427/140‑1 (MQ=255)
                                                                                                                  tttCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCATg                           <  1:3266136/140‑1 (MQ=255)
                                                                                                                    tCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCATGaa                         >  1:663848/1‑140 (MQ=255)
                                                                                                                     ccGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCATGAAg                        >  2:6903255/1‑140 (MQ=255)
                                                                                                                                 gTCATGCCTTTTTTTATTTTATCCTCGATAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCATGAAGTATTTGGGCAAg            <  2:3731605/140‑1 (MQ=255)
                                                                                                                                 gTCATGCCGGGTTTGATATTATCCGCGCTAACAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCATGAAGTATTTGGGCAAg            <  1:5771442/140‑1 (MQ=255)
                                                                                                                                 gTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGACTCGTAATATGTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCATGAAGTATTTGGGCAAg            <  1:4438016/140‑1 (MQ=255)
                                                                                                                                 gTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCATGAAGTATTTGGGCAAg            <  1:4951403/140‑1 (MQ=255)
                                                                                                                                 gTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCATGAAGTATTTGGGCAAg            <  1:5004217/140‑1 (MQ=255)
                                                                                                                                 gTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCATGAAGTATTTGGGCAAg            <  1:2780989/140‑1 (MQ=255)
                                                                                                                                 gTCATGCCGGGTTGGATCTTAGCCGCGCTAAAAGGAAGCATCGTACTATTTCTGTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCATGAAGTATTTGGGCAAg            <  1:6804763/140‑1 (MQ=255)
                                                                                                                                  tCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCATGAAGTATTTGGGCAAGc           <  1:1366268/140‑1 (MQ=255)
                                                                                                                                   cATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCATGAAGTATTTGGGCAAGCg          <  1:7583873/140‑1 (MQ=255)
                                                                                                                                    aTGCCGGTTTTTATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCATGAAGTATTTGGGCAAGCgg         <  2:2884410/140‑1 (MQ=255)
                                                                                                                                      gCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCATGAAGTATTTGTGCAAGCGGAc       >  2:1264736/1‑140 (MQ=255)
                                                                                                                                      gCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCATGAAGTATTTGGGCAAGCGGAc       >  2:1770289/1‑140 (MQ=255)
                                                                                                                                       ccGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCATGAAGTATTTGGGCAAGCGGACt      <  1:4033274/140‑1 (MQ=255)
                                                                                                                                          ggTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTg                                                >  1:6535479/1‑95 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ACGGCAACACTAGTGAATTTTTTCATGTAGTCGCCTAAAAACTTATGATAACGTATCACCGGAATTCCGTCAGACATGGCTTTTTCAATTTCAGGATGCGTGTCTGGGAATGCGTTTCCGGCGATGACTGTCATGCCGGGTTTGATATTATCCGCGCTAAAAGGAAGAATCGTAATATTTCTTTTTTCAAGCGCTGTTTGCGTAAAAATAAATTTTTCGATATCCGATCCTTGGACAGTATATCCATTATCATGAAGTATTTGGGCAAGCGGACTCATA  >  NZ_CP034943/2851155‑2851433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: