BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,865,416	C→A	6.8%	P134P (CCG→CCT)	pulA ←	type I pullulanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,865,416	0	C	A	6.8%	1040.8 / 5.9	474	P134P (CCG→CCT)	pulA	type I pullulanase
Reads supporting (aligned to +/- strand): ref base C (153/232); new base A (55/2); total (240/234)
Fisher's exact test for biased strand distribution p-value = 1.22e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 481 total aligned reads displayed.

ACGGGTCAACCGTTTCTGTCCATTCTGAATTGTTGCAGATGCAGAACACATATTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCGCGC  >  NZ_CP034943/2865277‑2865555

 cGGGTCAACCGTTTCTGTCCATTCTGAATTGTTGCAGATGCAGAACACATATTCATAACCGTGAAGATCACCGTTGACCGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCg                                                                                                                                            >  2:3294160/1‑140 (MQ=255)

 cGGGTCAACCGTTTCTGTCCATTCTGAATTGTTGCAGATGCAGAACACATATTCATAACCGTGAAGATCACCGTTGACAGTAAAGTTATAGACGCCTTTTTCCATCGGAATCATTTGGAAGATGCGGCCGCTTTTATTct                                                                                                                                            >  2:7201991/1‑139 (MQ=255)

    gTCAACCGTTTCTGTCCATTCTGAATTGTTGCAGATGCAGAACACATATTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGAt                                                                                                                                         >  2:5332289/1‑140 (MQ=255)

    gTCAACCGTTTCTGTCCATTCTGAATTGTTGCAGATGCAGAACACATATTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGAt                                                                                                                                         >  2:2618971/1‑140 (MQ=255)

       aaCCGTTTCTGTCCATTCTGAATTGTTGCAGATGCAGAACACATATTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGGTggg                                                                                                                                      >  1:1105710/1‑140 (MQ=255)

       aaCCGTTTCTGTCCATTCTGAATTGTTGCAGATGCAGAACACATATTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCGTTTATTAGGGTggg                                                                                                                                      >  2:1358801/1‑140 (MQ=255)

              tCTGTCCATTCTGAATTGTTGCAGATGCAGAACACATATTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTAGGCTTGGAAAGCtt                                                                                                                               >  1:5972277/1‑140 (MQ=255)

                 gTCCATTCTGAATTGTTGCAGATGCAGAACACATATTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGAc                                                                                                                            <  2:4745380/140‑1 (MQ=255)

                  tCCATTCTGAATTGTTGCAGATGCAGAACACATATTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGAca                                                                                                                           <  2:1878300/140‑1 (MQ=255)

                       tCTGAATTGTTGCAGATGCAGAACACATATTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGACTATGCGGCCGCTTTTATTAGGATGGGAAAGCTTGACagcagc                                                                                                                      >  1:7318576/1‑140 (MQ=255)

                       tCTGAATTGTTGCAGATGCAGAACACATATTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACagcagc                                                                                                                      >  1:4505342/1‑140 (MQ=255)

                            aTTGTTGCAGATGCAGAACACATATTCATAACCGTGAAGATCACCGTTGATAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTAGGATGGGAAAGCGTGACAGCAGCAGAgg                                                                                                                 >  1:5092902/1‑140 (MQ=255)

                                ttgCAGATGCAGAACACATATTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGACGCCTTTGGAATATGCGGCCGCTTTTATTGGGATGGGCAAGCTTGACAGCAGCTGAGGTTGc                                                                                                             >  2:303865/1‑140 (MQ=255)

                                ttgCAGATGCAGAACACATATTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTGGGCTGGGGAAGCTTGACAGCCGCTGTGGTTgg                                                                                                             >  1:100988/1‑139 (MQ=255)

                                ttgCAGATGCAGAACACATATTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTGGGAATGGAAAGCGATCAAGCAGCTGAGGTTGc                                                                                                             >  1:7005726/1‑140 (MQ=255)

                                ttgCAGATGCAGAACACATATTCATAACCGGGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCAGCGGACTCAGTTGGAATATGCGGCCGCTTTTATTGGGATGGGAAAGCTGGTCAGCAGCTGAGGGTGc                                                                                                             >  1:6131437/1‑140 (MQ=255)

                                    agatgcagaACACATATTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCa                                                                                                         <  2:1977646/140‑1 (MQ=255)

                                    agatgcagaACACATATTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCa                                                                                                         <  1:2763825/140‑1 (MQ=255)

                                    agatgcagaACACATATTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCa                                                                                                         <  2:5857981/140‑1 (MQ=255)

                                    agatgcagaACACATATTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCa                                                                                                         <  2:4828255/140‑1 (MQ=255)

                                         cagaACACATATTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCGGAGGTTGCGGCAGgcgc                                                                                                    >  2:1176719/1‑140 (MQ=255)

                                                 atatTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGTAAGCTTGACAGCCGCAGAGGTTGCGGCAGGCGCCCATACtg                                                                                            >  1:1662820/1‑139 (MQ=255)

                                                 atatTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACtt                                                                                            >  2:642600/1‑140 (MQ=255)

                                                 atatTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCAGAGGTTTCGGCAGGCGCCCATACtt                                                                                            >  2:6727230/1‑140 (MQ=255)

                                                   atTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTTACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTa                                                                                          >  1:3772703/1‑140 (MQ=255)

                                                      cATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTTCGGCAGGCGCCCAAACTTTaaaa                                                                                       >  1:237784/1‑140 (MQ=255)

                                                       aTAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAAt                                                                                      <  1:4339941/140‑1 (MQ=255)

                                                       aTAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAAt                                                                                      <  1:4652050/140‑1 (MQ=255)

                                                        tAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATc                                                                                     >  2:3186425/1‑140 (MQ=255)

                                                         aaCCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTGGGATGGGAAACCTTGTCAGCAGCTGAGGTTGCGGCAGGCGCCCCAACATTaaaaaaa                                                                                    >  1:7079924/1‑137 (MQ=255)

                                                         aaCCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGa                                                                                                                                     <  1:1903508/91‑1 (MQ=255)

                                                           ccGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTAGGATGGGAAACCTTGACAGCAGCTGAGGTTTCGGCAGGCGCCCCGACTTTAAAATCAGt                                                                                  >  1:3494528/1‑140 (MQ=255)

                                                           ccGTGAAGATAACCGTTCACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGATTATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGt                                                                                  <  2:4452871/140‑1 (MQ=255)

                                                              tGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTTTTCGGATGGGAAAGCTTTACAGCCGCTGAGGGTTCGGCAGGCGCCCAGACATTAAAATCATtata                                                                               >  1:1939316/1‑140 (MQ=255)

                                                              tGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTGGGATTGGAAAGCTTGACAGCAGCTGAGGTTTGGGCCGGCGCCCCAAATTTAAAATAAGtata                                                                               >  1:4811889/1‑140 (MQ=255)

                                                              tGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTTACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGtata                                                                               >  2:2230020/1‑140 (MQ=255)

                                                              tGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGtata                                                                               >  2:4357266/1‑140 (MQ=255)

                                                              tGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCGTTTATATGTATGGGGAAACATTACAACAGCGGAGGTTGAGGCAGGCCCCCAAAATTTAAAATAAAtata                                                                               >  1:4895740/1‑140 (MQ=255)

                                                                aaGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTGGGATGGGAAAGCTTGTCAGCAGCTGAGGTTGCGGCAGGCGCCCCTACCTTAAAATCACTCTAAt                                                                             >  1:6325460/1‑140 (MQ=255)

                                                                  gATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCTGCAGGCGCCCATACTTTAAAATCAGTATAATTc                                                                           <  1:5546000/140‑1 (MQ=255)

                                                                  gATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTc                                                                           <  1:6268168/140‑1 (MQ=255)

                                                                  gATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTc                                                                           <  2:6637420/140‑1 (MQ=255)

                                                                  gATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTc                                                                           <  1:6972114/140‑1 (MQ=255)

                                                                  gATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTc                                                                           <  2:647812/140‑1 (MQ=255)

                                                                  gATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTc                                                                           <  1:6524345/140‑1 (MQ=255)

                                                                  gATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTc                                                                           <  2:6370646/140‑1 (MQ=255)

                                                                  gATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGGTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTc                                                                           <  2:2737616/140‑1 (MQ=255)

                                                                   aTCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCg                                                                          <  1:2899769/140‑1 (MQ=255)

                                                                   aTCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCg                                                                          <  1:3653550/140‑1 (MQ=255)

                                                                   aTCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCg                                                                          <  2:3576143/140‑1 (MQ=255)

                                                                    tCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATCTGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGc                                                                         <  1:743733/140‑1 (MQ=255)

                                                                    tCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGc                                                                         <  1:7763808/140‑1 (MQ=255)

                                                                    tCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGc                                                                         <  1:7385721/140‑1 (MQ=255)

                                                                      aCCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTTTTGGGGTGGGAAACCTTGACAGCCGCTGAGGTTGGGGCAGGCGCCCAGACTTTAAAATAACTATAAGTCGcgg                                                                       >  1:3076448/1‑138 (MQ=255)

                                                                      aCCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCGGAGGTTTCGGCAGGCGCCCATACTTTCAAATCAGTATAATTCGCAg                                                                       >  2:7407494/1‑140 (MQ=255)

                                                                        cGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGGTGGGGAAGCTTGACAGCAGCGGAGGTTTCGGCAGGCGCCCCTACATTAAAATCAGTATAATTCGCAGta                                                                     >  1:393717/1‑140 (MQ=255)

                                                                              cAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACa                                                               <  1:4357266/140‑1 (MQ=255)

                                                                              cAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACa                                                               <  1:3362045/140‑1 (MQ=255)

                                                                               aGTAACGGTATAGACGCCTTTTTCCATCCGAAGCATTTGGAATATGCGGCCGCTTTTATTAGGCTGGGAAAGCGTGTCACCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAAACCGTATAATTCGGAGTATAAACAg                                                              >  2:6869979/1‑140 (MQ=255)

                                                                                  aaCGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTTTTCGGGTGTGAAAGCGTTAAACCAGCGGAGGTTGGGGCAGGCGCCCAAAACTTAAAATAAGTATAAATAGCACTATAAAAAGccc                                                           >  1:526989/1‑140 (MQ=255)

                                                                                  aaCGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTGTGTTGGGGAAGCTTGTCCGCCGCGGTGGGTTGGGCCGGCGCCCATAATTTAAAATAAGTATAATTAGCAGGATAAAAAAccc                                                           >  1:675036/1‑140 (MQ=255)

                                                                                  aaCGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCGTTTATTCGGATGGGAAACCTTGACAGCAGCGGAGGTTGGGGCACGCGCCCAAACTTTAAAATAACTATAAATCGCACTAAAAAAAGccc                                                           >  1:4026310/1‑140 (MQ=255)

                                                                                   aCGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGcccc                                                          <  2:1598021/140‑1 (MQ=255)

                                                                                   aCGGTATAGACGCCTTTTTCCATCCGAAACATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGcccc                                                          <  2:3810756/140‑1 (MQ=255)

                                                                                      gTATAGACGCCTTTTTCGATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAg                                                       <  2:7638965/140‑1 (MQ=255)

                                                                                       tataGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTGGGTTGGGAAACCTTGACAGCAGCTGAGGTTTCGGCCGGCGCCCATACATTAAAATCAGTATAAATCGCAGGATAAAAACCCCCCAGc                                                      >  1:7545022/1‑140 (MQ=255)

                                                                                       tataGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGAAAGCAGCGGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGc                                                      >  2:1427643/1‑140 (MQ=255)

                                                                                        ataGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGct                                                     <  1:343148/140‑1 (MQ=255)

                                                                                         taGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATAATTTAAAATAAGTATAATTCGCAGTATAAACAGCCCCCAGctc                                                    >  2:2509171/1‑140 (MQ=255)

                                                                                           gACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATAAGTATAATTCGCCGTATAAACAGCCCCCAGCtctc                                                  >  2:5986218/1‑140 (MQ=255)

                                                                                              gCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGt                                               <  2:1017044/140‑1 (MQ=255)

                                                                                               ccTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCACCGGCGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCACTATAAAAAGCCCCCAGCTCTACGTc                                              >  2:6277214/1‑140 (MQ=255)

                                                                                               ccTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCGTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGGGGCAGGCGCCCATACTTTAAAATCAGTATAATTAGCAGTATAAACAGACCCCAGCTCTCCggc                                              >  2:3930609/1‑138 (MQ=255)

                                                                                                 tttttCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGc                                                                         <  1:5710783/111‑1 (MQ=255)

                                                                                                 tttttCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGc                                                                         >  2:5710783/1‑111 (MQ=255)

                                                                                                 tttttCCATCCGAATCATTTGGAATATGCGGCCGCGTTTATTCGGATGGGGAAGCTTGACCGCAGCTGAGGTTTGGGCCGGCGCCCCTACTTTAAAATAAGGATAATTCGCAGTATAAAAAACCCCCCGCTCGCCggcct                                            >  1:4465403/1‑136 (MQ=255)

                                                                                                 cttttCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCCGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGc                                                                         >  2:3395837/2‑111 (MQ=255)

                                                                                                   tttCCATCCGAATCATTTGGAATATGCGGCCGCGTTTATTAGGGTGGGGAACCGTGACACCAGCGGAGGGTGCGGCACGCGCCCAAACGTTAAAATAAAGATAATTAGCAGTATAAAAACCCCCCACCTCTACgggaaaa                                          >  1:6438628/1‑134 (MQ=255)

                                                                                                          ccGAATCATTTGGAATATGCGGCCGCTTTTTTTAGGATTGGTAACCTTGAAAGCACCGGAGGTTTCGGCAGGCGCCCCTACTTTAAAATCACTATAAATCGCACTATAAAAACCCCCCACCTCTCCGTCATAAAAAAATg                                   >  1:215408/1‑140 (MQ=255)

                                                                                                          ccGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTAGAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATg                                   <  1:3294160/140‑1 (MQ=255)

                                                                                                          ccGAATCATTTGGAATATGCGGCCGCTTTTATTAGGATGGGAAACCTTGACAGCAGCGGAGGTTGCGGCAGGCGCCCATAATTTAAAATAAATATAAATCGCAGTATAAAAAGCCCCCACCTCAACGTCATAATAAAATg                                   >  2:1263594/1‑140 (MQ=255)

                                                                                                           cGAATCATTTGGAATATGCGGCCGCTTTTCTTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGa                                  <  2:4659464/140‑1 (MQ=255)

                                                                                                           cGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGa                                  <  1:5470247/140‑1 (MQ=255)

                                                                                                           cGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGa                                  <  1:2583384/140‑1 (MQ=255)

                                                                                                              aTCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAatc                               <  2:292167/140‑1 (MQ=255)

                                                                                                              aTCATTTGGAATATGCGGCCGCGTTTATTAGGATGGGAAAACATTACACCACCTGAGGTTGAGGCAGGCGCCCAAACATTAAAATAAGTATAAATAGCAGTATAAACAACCCCCCCCTCTCCCGCAAAATAAAATTAatc                               >  1:6564251/1‑140 (MQ=255)

                                                                                                                   ttGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCaa                          <  1:72404/140‑1 (MQ=255)

                                                                                                                    tGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCaaa                         <  2:2157578/140‑1 (MQ=255)

                                                                                                                         tatGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTAt                    <  2:7523681/140‑1 (MQ=255)

                                                                                                                         tatGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTAt                    <  1:1300088/140‑1 (MQ=255)

                                                                                                                         tatGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTAt                    <  2:6968638/140‑1 (MQ=255)

                                                                                                                           tGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCg                  <  1:4221615/140‑1 (MQ=255)

                                                                                                                           tGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCg                  <  1:1463939/140‑1 (MQ=255)

                                                                                                                           tGCGGCCGCTTTTATTCGGATGGGAAACCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACGTTAAAAACAGGATAATTCGCAGGCTAAACAACCCCAAGCACACCCTCATAATAAAATGAATAATCAAACGTAACg                  >  2:5095602/1‑140 (MQ=255)

                                                                                                                            gCGGCCGCTTTTATTCCGCTGGGAAAGCTTGACAGCAGCTGAGGGTTCGGCTGGCGCCCATAGTTTAAAATCAGTATACTTCGGAGTATAAACAGCCCCCAGCACTGCGTCATAATAAAATGAATAATCAAACGTATCgg                 >  2:5461650/1‑140 (MQ=255)

                                                                                                                             cGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGt                <  2:4968007/140‑1 (MQ=255)

                                                                                                                             cGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGt                <  2:4274981/140‑1 (MQ=255)

                                                                                                                                 cGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCCGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCgg            >  2:1778183/1‑140 (MQ=255)

                                                                                                                                 cGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTAGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATAAAACGTATAGGTCCgg            >  2:6231212/1‑140 (MQ=255)

                                                                                                                                      ttATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGAc       <  2:4070398/140‑1 (MQ=255)

ACGGGTCAACCGTTTCTGTCCATTCTGAATTGTTGCAGATGCAGAACACATATTCATAACCGTGAAGATCACCGTTGACAGTAACGGTATAGACGCCTTTTTCCATCCGAATCATTTGGAATATGCGGCCGCTTTTATTCGGATGGGAAAGCTTGACAGCAGCTGAGGTTGCGGCAGGCGCCCATACTTTAAAATCAGTATAATTCGCAGTATAAACAGCCCCCAGCTCTCCGTCATAATAAAATGAATCATCAAACGTATCGGTCCGGATGACCGCGC  >  NZ_CP034943/2865277‑2865555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`