Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,900,726 G→T 5.2% G96V (GGA→GTA)  EO946_RS14945 → extracellular solute‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,900,7260GT 5.2% 1347.9 / 11.5 468G96V (GGA→GTA) EO946_RS14945extracellular solute‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (135/286);  new base T (2/38);  total (137/331)
Fisher's exact test for biased strand distribution p-value = 9.87e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 485 total aligned reads displayed.

ACTGGCGGCAGCGTTTGAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGG  >  NZ_CP034943/2900589‑2900864
                                                                                                                                         |                                                                                                                                          
  tGGCGGCAGCGTTTGAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGaaaa                                                                                                                                        >  1:4355840/1‑140 (MQ=255)
       gcAGCGTTTGAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAg                                                                                                                                   <  2:2505333/140‑1 (MQ=255)
           cGTTTGAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTAt                                                                                                                               <  1:1212723/140‑1 (MQ=255)
           cGTTTGAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTAt                                                                                                                               <  1:5348591/140‑1 (MQ=255)
           cGTTTGAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTAt                                                                                                                               <  2:6753783/140‑1 (MQ=255)
           cGTTTGAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTAt                                                                                                                               <  1:1840089/140‑1 (MQ=255)
           cGTTTGAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTAt                                                                                                                               <  1:806922/140‑1 (MQ=255)
           cGTTTGAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTAt                                                                                                                               <  1:198107/140‑1 (MQ=255)
           cGTTTGAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTAt                                                                                                                               <  2:2332048/140‑1 (MQ=255)
           cGTTTGAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTAt                                                                                                                               <  2:4733221/140‑1 (MQ=255)
           cGTTTGAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTAt                                                                                                                               <  1:247524/140‑1 (MQ=255)
           cGTTTGAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTAt                                                                                                                               <  1:2995047/140‑1 (MQ=255)
           cGTTTGAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATGTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTAt                                                                                                                               <  1:4906745/140‑1 (MQ=255)
           cGTTTGAAAAGGAACACAGAGACCTTCGTGTCAGCCGTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCATTTCGATACACATGGCTGGGGAAAAATTAGGTAt                                                                                                                               <  1:1041325/140‑1 (MQ=255)
             tttGAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATgg                                                                                                                             <  2:279821/140‑1 (MQ=255)
             tttGAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATgg                                                                                                                             <  2:4605468/140‑1 (MQ=255)
              ttGAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATTATGCGTGTCAGAATGGCAGCCAATGATTTGCATGACCTTTTCGATACACATGGATGGGGAAAAATTAGGTATGga                                                                                                                            <  1:3180406/140‑1 (MQ=255)
              ttGAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGGGTGTCAGAATGGCAGCCAATGATTTGCCTGACGTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGga                                                                                                                            <  2:2025100/140‑1 (MQ=255)
              ttGAAAAGGAACACAGACACCTTCGTGTCAGCCTTCATGTCCCAGGCAATGACTATGAAAATATGATGCGTGACAGAATGGCAGCCCATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTAAGga                                                                                                                            <  1:3840970/140‑1 (MQ=255)
                gAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGgaga                                                                                                                          <  2:3140054/140‑1 (MQ=255)
                gAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGgaga                                                                                                                          <  2:3620699/140‑1 (MQ=255)
                       aaCACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACa                                                                                                                   <  2:6616682/140‑1 (MQ=255)
                        acacAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAg                                                                                                                  <  1:3991098/140‑1 (MQ=255)
                          acaGAGACCTTCTGGTCACTCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACAGGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCg                                                                                                                <  2:5862482/140‑1 (MQ=255)
                          acaGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCg                                                                                                                <  2:6014413/140‑1 (MQ=255)
                              agaCCTTCGTGTCAGCCTTCATTTCCCAGGCCATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAAGGATTTGCCTGACCTTTTCGATACACAGGGCTGGGGAAACATTAGGTATGGAGAATATACAGCGGAtc                                                                                                            >  2:908907/1‑140 (MQ=255)
                              agaCCTTCGTGTCAGCCTTCATTTCCCAGGCACTGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGAtc                                                                                                            >  2:3232298/1‑140 (MQ=255)
                              agaCCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGAtc                                                                                                            >  1:5084248/1‑140 (MQ=255)
                              agaCCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGAtc                                                                                                            >  1:6149087/1‑140 (MQ=255)
                                aCCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAACTATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTATTCGATACACATGGCGGGGGAAAAATTAGGTATGGAGAATATACCGCGGAtctc                                                                                                          >  2:1510179/1‑140 (MQ=255)
                                 ccTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCa                                                                                                         <  1:7325184/140‑1 (MQ=255)
                                 ccTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCa                                                                                                         >  2:3482473/1‑140 (MQ=255)
                                 ccTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCa                                                                                                         <  2:4643450/140‑1 (MQ=255)
                                 ccTTCGTCTCAGCCTTCATTTCCCATGCAATGACTATGAAAATATGATGCGTGTCAGAATGTCACCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGACTATACAGCGGATCTCa                                                                                                         <  1:1360982/140‑1 (MQ=255)
                                 ccTTCGAGTCCGCCTTCATTTCCCAGGCACTGACTATGAAACTATGAGGCGTGTCAGAAGGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAACTAGGTATGGAGAATATACAGCGGATCTCa                                                                                                         <  1:921162/140‑1 (MQ=255)
                                     cGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAgaga                                                                                                     >  2:5273507/1‑140 (MQ=255)
                                       tgtCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGata                                                                                                   <  1:5960807/140‑1 (MQ=255)
                                           aGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTAt                                                                                                                               >  1:3350361/1‑108 (MQ=255)
                                            gCCTTCATTTCCCAGGCAATGACTATGAACATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGACTATACAGCGGATCTCAGAGATATGGGa                                                                                              >  2:1240133/1‑140 (MQ=255)
                                            gCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGa                                                                                              <  1:1589743/140‑1 (MQ=255)
                                            gCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGa                                                                                              >  1:4898402/1‑140 (MQ=255)
                                             ccTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGAt                                                                                             <  2:7477402/140‑1 (MQ=255)
                                             ccTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAAGGGCAGCCAATGATGTGCCTGACCTTTTCGATCCACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGAt                                                                                             <  2:1172624/140‑1 (MQ=255)
                                              cTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATg                                                                                            <  1:4520848/140‑1 (MQ=255)
                                                  aTTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGata                                                                                                   <  1:2069270/129‑1 (MQ=255)
                                                  aTTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGata                                                                                                   >  2:2083535/1‑129 (MQ=255)
                                                    ttCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCa                                                                                      <  1:98857/140‑1 (MQ=255)
                                                        cAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGAt                                                                                  <  2:1871529/140‑1 (MQ=255)
                                                           gCACTGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGTAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGAtctc                                                                               >  1:2293921/1‑140 (MQ=255)
                                                               tGACTATGAAAATATGATGCGTGTCAGAATGGCGGCCAATGATTTGCCAGATCTATTCGATACACATGGCTGGGGGAAAATCAGGTACGGAGAATATACAGCGGATCTCCGGGATATGAAATGGACTCAAGATCTTGATc                                                                           <  1:2784751/140‑1 (MQ=255)
                                                               tGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATc                                                                           <  1:5304276/140‑1 (MQ=255)
                                                               tGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATc                                                                           <  1:2188327/140‑1 (MQ=255)
                                                               tGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATc                                                                           <  1:5782962/140‑1 (MQ=255)
                                                                     tGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAAtt                                                                     >  1:36211/1‑140 (MQ=255)
                                                                     tGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAAtt                                                                     >  2:2665270/1‑140 (MQ=255)
                                                                        aaaTATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTaa                                                                  <  2:3731279/140‑1 (MQ=255)
                                                                          atatGATGCGTGTCAGAATGGCAGCCAATGATTTCCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTGAAAc                                                                >  2:5450958/1‑140 (MQ=255)
                                                                                    tgtCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCa                                                      >  1:4251867/1‑140 (MQ=255)
                                                                                    tgtCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCa                                                      >  2:4904650/1‑140 (MQ=255)
                                                                                    tgtCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCa                                                      >  2:424529/1‑140 (MQ=255)
                                                                                               gaGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAACAGATCGATCCCAATTTAAAGAGCTTCCTCAAACAGaaaatc                                           >  2:7033186/2‑138 (MQ=255)
                                                                                               cAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGga                                                                                                                            >  1:1923063/1‑59 (MQ=255)
                                                                                               cAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGc                                           >  1:4774019/1‑140 (MQ=255)
                                                                                               cAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGc                                           >  2:4051199/1‑140 (MQ=255)
                                                                                                  ccAATTTTTTGCCTTAAATTTTCGATACACTTGGCTGGTTAAAAATTATGTATGGAGAATATACAGCGGATCTCAGAGATATGGTATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGc                                        <  2:6173980/140‑1 (MQ=255)
                                                                                                  ccAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGc                                        <  2:4399921/140‑1 (MQ=255)
                                                                                                  ccAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTAAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGc                                        <  2:1927944/140‑1 (MQ=255)
                                                                                                   catttttttGCCTGACCTTTTCGATACACATGGCTGGGTAAAATTTAGGTATGGATAATATACAGCGTATCTCAGAGATATGGGATGGACCAAATATATCGATCCCATTTTAAACAGCATCCTCAAAAATAAAAGCGGCa                                       <  1:5521662/134‑1 (MQ=255)
                                                                                                   cattgttttGCGTGACCTTTTCGATACACATGGCTGGTTAAAATTTTGTTTTGGATATTATACCGCGGATCTCAGAGTTTTGGGTTGCACCAAATATCTCGATCCAAATTTAAACAGAATCCTAAAAAATAAAAGCGGCa                                       <  1:6861029/134‑1 (MQ=255)
                                                                                                   cAATTATTTGCCTGACATTTTCGATACACATGGCTGGGAAAAAATTAGGTATGGAGAATATACACCGCATCTCAGACATATGGGATGCACCCAAGATCTCGATCCCAATTTAAACAGCATCAACAAAAAAAAACGCGGCa                                       <  1:548339/140‑1 (MQ=255)
                                                                                                   cAATTATTTGCCTCACCTTTACGATAAACTTGGCTGGGGAAAATTTCGGTATGGATAATATACAGCGTATCTCAGAGATATGGGTTGCACCCAAGATCTCTATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCa                                       <  1:6947722/140‑1 (MQ=255)
                                                                                                   cAATGATTTGCCTGACGTTTGCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAACGCGGCa                                       <  2:3420440/140‑1 (MQ=255)
                                                                                                   cAATGATTTGCCTGACCTTTTCGATACAGATGGCTGGGGAAAAATTTGTTATGGAGATTATACTGCGGTTCTCTTATATATGGGTTGGACCAATGATCTCGATCCCAATTTAAAACGCATCCTAAAAAATAAAAGCGGCa                                       <  2:2196691/140‑1 (MQ=255)
                                                                                                   cAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTTTGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCa                                       <  2:3853740/140‑1 (MQ=255)
                                                                                                   cAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCa                                       <  2:7594606/140‑1 (MQ=255)
                                                                                                   cAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCa                                       <  2:1821199/140‑1 (MQ=255)
                                                                                                   cAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCa                                       <  2:7687119/140‑1 (MQ=255)
                                                                                                   cAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGACATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCACAAAAATAAAAGCGGCa                                       <  2:6985643/140‑1 (MQ=255)
                                                                                                   cAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTAGGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCa                                       <  2:6325092/140‑1 (MQ=255)
                                                                                                   cAATGATTTGCCTGACATTTTCTATACAAATGGGTGGGGAAAATTTAGGTATGGAGAATATAATGCGGATCTCAGAGATATGGGATGGACCCAACATCTCGATCCCAATTTAAACAGAATCCTCAAAAATAACAGCGGCa                                       <  2:1682755/140‑1 (MQ=255)
                                                                                                   cAATGATTTGCCTGAACTTTTCTATAAAAATGGCTGGGTAAAAATTTGTTATGTAGAATATACTGCGGATCTCCTATATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACCGCATCCACAAAAATAAAAGCGGCa                                       <  1:1736055/140‑1 (MQ=255)
                                                                                                   cAATGATGTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAACATAAAAGCGGCa                                       <  2:1417414/140‑1 (MQ=255)
                                                                                                   cAATGATGTGCCTGACCTTTACCTTACACAGGGCTGGCTAAAAATTAGGTATGTACAATATACAGCGGAACACACAGATTTGGTATGTACCCAAGATCTCGATCCAAATTTAAACAGCAGCCTCAAAAATAAAAGCGGCa                                       <  1:2591779/140‑1 (MQ=255)
                                                                                                   cAATGAGTTGCCTGACCTTTTCGATACACCTGGCTGGGGAAAAATTAGGTATTGAGAATATACAGGTTATCTCAGTGATATGGGATGGACCCAAGATCTCGTTCCCAATTTAAACAGCATCCTCAAAAAAAAACGCGGCa                                       <  2:5104765/140‑1 (MQ=255)
                                                                                                   aatttaTTTGCCTGACTTTTTCGATACACTTGGGTGGTTAAAAATTAGTTTTGGAGAATATACAGCGGATCTCAGAGATTTGGGAGGGACCAAATATCTCGATCCCAATTTAAACAGCATCCTAAAAAATAAACGCGGCa                                       <  1:4989001/135‑1 (MQ=255)
                                                                                                   aaaTTATTTGAGTGACCTTTTCTATACCAATGGCTGTGTAAAATTTAGTTTTGGATAATATACAGCGTATCTAATATATATGGTTGTGACCAAATATCTCTATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCa                                       <  2:5968340/139‑1 (MQ=255)
                                                                                                   aaaTGATTTGCCTGACCTTTTCTATACAAATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTAAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCa                                       <  2:7141117/139‑1 (MQ=255)
                                                                                                        aTTTGCCTTACCTTTTCGATACACATGGCTGGGGAAAAATTAGTTTTGGATAATATACAGCGTATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGta                                  <  2:5017834/140‑1 (MQ=255)
                                                                                                         tttGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGtat                                 <  2:5216863/140‑1 (MQ=255)
                                                                                                                 aCCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTAc                         >  1:7067661/1‑140 (MQ=255)
                                                                                                                      ttCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGat                    >  1:2930553/1‑140 (MQ=255)
                                                                                                                           tACACATGGCTGGGGAAAAATTAGGTCTGGATAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGatcaatc               <  2:5565124/140‑1 (MQ=255)
                                                                                                                              acaTGGCGGGGCAAAAATTCGGTAGGCAGAATATACAGCGCATAGAAGAGATATGGCAGGCACCCAAGATATCGAGCCAAATTAAAACAGCACCCTCAAAAACAACCGCGGCAAGGTATATGCCTACCCGATCAATCAgg            <  1:3469077/140‑1 (MQ=255)
                                                                                                                               caTGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGc           >  1:6540991/1‑140 (MQ=255)
                                                                                                                                aTGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCa          >  1:5919123/1‑140 (MQ=255)
                                                                                                                                     tGGGGAAAAATTAGGTATGGAGAATATACAGCGTATCTCAGAGATATGGTATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAACGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGa     <  1:6313902/140‑1 (MQ=255)
                                                                                                                                       gggAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACg   >  2:5791033/1‑140 (MQ=255)
                                                                                                                                       gggAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACg   >  2:7251664/1‑140 (MQ=255)
                                                                                                                                       gggAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACg   >  2:5652282/1‑140 (MQ=255)
                                                                                                                                        ggAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACgg  >  1:7405043/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                          
ACTGGCGGCAGCGTTTGAAAAGGAACACAGAGACCTTCGTGTCAGCCTTCATTTCCCAGGCAATGACTATGAAAATATGATGCGTGTCAGAATGGCAGCCAATGATTTGCCTGACCTTTTCGATACACATGGCTGGGGAAAAATTAGGTATGGAGAATATACAGCGGATCTCAGAGATATGGGATGGACCCAAGATCTCGATCCCAATTTAAACAGCATCCTCAAAAATAAAAGCGGCAAGGTATATGCCTACCCGATCAATCAGGCAAAAGACGG  >  NZ_CP034943/2900589‑2900864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: