Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 177,029 G→C 13.3% intergenic (+202/‑70) EO946_RS01020 → / → EO946_RS01025 alcohol dehydrogenase/SulP family inorganic anion transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943177,0290GC13.3% 759.4 / 24.3 325intergenic (+202/‑70)EO946_RS01020/EO946_RS01025alcohol dehydrogenase/SulP family inorganic anion transporter
Reads supporting (aligned to +/- strand):  ref base G (141/135);  new base C (46/3);  total (187/138)
Fisher's exact test for biased strand distribution p-value = 2.91e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 325 total aligned reads displayed.

CCATTATTTTCTTCATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCA  >  NZ_CP034943/176891‑177165
                                                                                                                                          |                                                                                                                                          
ccATTATTTTCTTCATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGt                                                                                                                                           <  2:5065312/140‑1 (MQ=255)
    tattTTCTTCATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTTCTTTCATTTATACCGTaaaa                                                                                                                                       >  1:6869006/1‑140 (MQ=255)
         tcttcATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTAATTTATACCCTaaaaaaaaa                                                                                                                                  >  2:3296163/1‑139 (MQ=255)
          cttcATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATaaaga                                                                                                                                 <  1:824228/140‑1 (MQ=255)
          cttcATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATaaaga                                                                                                                                 <  1:4207886/140‑1 (MQ=255)
          cttcATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATaaaga                                                                                                                                 <  1:170332/140‑1 (MQ=255)
          cttcATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCGTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATaaaga                                                                                                                                 <  2:7123537/140‑1 (MQ=255)
            tcatcaTAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTTCTTTGATTTCTACCGTAAAAtaaataaa                                                                                                                               >  2:3134594/1‑140 (MQ=255)
             catcatAGATCTTGCGTATTTGATCCAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTAATTTGTACCGTCAAATaaagaaac                                                                                                                              >  1:5863842/1‑139 (MQ=255)
             catcatAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGGTTTGATTTATACCCGAAAAAaaagaaac                                                                                                                              >  1:24158/1‑139 (MQ=255)
             catcatAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAAAaaagaaaa                                                                                                                              >  2:92461/1‑139 (MQ=255)
             catcatAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTATCCCGTAAAATaaagaaag                                                                                                                              >  2:3662661/1‑140 (MQ=255)
             catcatAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTAAATTATACCCTAAAATaaaaaaaa                                                                                                                              >  1:2133119/1‑139 (MQ=255)
               tcatAGATCTTGCGTATTTGATCAAAATTTAAGATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCt                                                                                                                            <  1:4758316/140‑1 (MQ=255)
                catAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTc                                                                                                                           <  1:7098824/140‑1 (MQ=255)
                catAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTc                                                                                                                           <  1:7255165/140‑1 (MQ=255)
                catAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCATTTCATTTATCCAGTAAAAAaaataaagata                                                                                                                           >  2:4721304/1‑137 (MQ=255)
                    tatattGAGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCaa                                                                                                                       <  2:3769809/136‑1 (MQ=255)
                    gATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCaa                                                                                                                       <  1:3134594/140‑1 (MQ=255)
                    gATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCaa                                                                                                                       <  1:1109654/140‑1 (MQ=255)
                     ttattGCGTATTTGATCAAAATTTAATATGATAATATTTGTTTAATTACTCCTTGCCTCAAAACAACATAATCAACGTGTACTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCACGTAAAATAAAGAAAGCTCCCAAt                                                                                                                      <  2:3794202/137‑1 (MQ=255)
                     aTCTTTCGTATTTGATCAAAATTTAATATGATACTATTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCAGTAAAATAAAGAAAGCTCCCAAt                                                                                                                      <  2:6493908/140‑1 (MQ=255)
                     aTCTTGCTTATTTTATAAAAATTTAATATTATACTATTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAAt                                                                                                                      <  2:4069988/140‑1 (MQ=255)
                     aTCTTGCGTATTTGATCAAACTTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTATTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAAt                                                                                                                      <  1:1415088/140‑1 (MQ=255)
                     aTCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAAt                                                                                                                      <  2:1828544/140‑1 (MQ=255)
                     aTCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAAt                                                                                                                      <  2:4950487/140‑1 (MQ=255)
                     aTCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAAt                                                                                                                      <  2:5777486/140‑1 (MQ=255)
                     aTCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAAt                                                                                                                      <  2:6187137/140‑1 (MQ=255)
                     aTCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAAt                                                                                                                      <  2:3256782/140‑1 (MQ=255)
                     aTCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAAt                                                                                                                      <  1:4498504/140‑1 (MQ=255)
                       cTTTCGTATTTGATGAAAATTTAATATGATACTATTTGTATAATAACTCCTTGACTCAAAACAACATACTCAACGTGTCCTCTTTTTATCCGTTCGTTTTCCTTTAATTTCTCCAGTAAAATAAAGAAAGCTCCCAATac                                                                                                                    <  2:7080234/140‑1 (MQ=255)
                       cTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATac                                                                                                                    <  1:768341/140‑1 (MQ=255)
                       attGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATac                                                                                                                    <  1:4581275/139‑1 (MQ=255)
                         tGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTCATTTCTCCCGTAAAATAAAGAAAGCTGCCAAAacaa                                                                                                                  >  2:644308/1‑140 (MQ=255)
                          gCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTAATTTGTCCCCTAAAATAAAGAAAACTCCCAATaaaaa                                                                                                                 >  1:287910/1‑139 (MQ=255)
                          gCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCGTTGATTTATCCCGTAAAAAAAAGAAAGCTCCCCATaaaaa                                                                                                                 >  1:5255447/1‑139 (MQ=255)
                          gCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAAAaaaac                                                                                                                 >  2:232233/1‑140 (MQ=255)
                          gCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTAATTTCTACCGTAAAATAAAAAAAGCTCCCacaaaaac                                                                                                                 >  1:2236770/1‑133 (MQ=255)
                          gCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAAAAACTCCTTGCCTCAAAACAACATAATCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTCATTACTAGCGATAAAAAAACAAAGCAACCAAAacaaa                                                                                                                 >  2:3896171/1‑139 (MQ=255)
                             tATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCAATTATCCCGTAAAATAAATAAAGCTCCCAATACAACaaa                                                                                                              >  2:7197687/1‑140 (MQ=255)
                               tttGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTCATTTATACCCTAAAAAAAAGAAAGCTCCCAAAACAAAAAATa                                                                                                            >  2:2264003/1‑140 (MQ=255)
                                    tCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTg                                                                                                      <  2:805254/140‑1 (MQ=255)
                                        aaTTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTAATTTGTACCGTAAAATAAAGAAAGATACCAATACAAAAAATAAGGTGCGGa                                                                                                  >  2:1964687/1‑140 (MQ=255)
                                             aaTATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCGTTTAGCCCGTAAAATAAAGAAATCTCCCAAAAAAAAAAATAAGGGGCGGttttat                                                                                             >  2:1889141/1‑140 (MQ=255)
                                                  gATACTCTTTGTATAATAACTCCTTGCCTCAATACAACATACTCAACGTGTCCTCTTTTTCTCGGGTCGTTTTGCTTTGATTTGTCCCGTAAAATATAGAAAGATCCCAATACAACAAATACGGGGCGGAGTTAtttttt                                                                                        >  2:5371991/1‑140 (MQ=255)
                                                    tACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTaa                                                                                      <  2:1018249/140‑1 (MQ=255)
                                                     aCTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATCCAACAAATACGGTGCGGATTTATTTTTTaaa                                                                                     <  2:1390143/140‑1 (MQ=255)
                                                        ctTTGTATAATAACTCCTTGCCTCAAACCAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAg                                                                                  <  1:3270857/140‑1 (MQ=255)
                                                         tttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCGTTGATTTGTCCCCTAAAATAAATAAAGCTCCCCAAAAAAAAAAAAAGGTGCGGCTTTATTTTTTaaaaatg                                                                                 >  1:5797285/1‑138 (MQ=255)
                                                          ttGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGa                                                                                <  2:531423/140‑1 (MQ=255)
                                                           tGTATAATAACTCCTTTCCTCAATACAACATACTCAACGTGTCCTCTTTTTCTCAGGTCGTTTTCCTGTGGTTTCTGGAGGAAAATAAAGACAGCTCGCCATACAACATAAACGCTGGGCATTTATTTGTTAAAAACGAg                                                                               >  1:6030613/1‑140 (MQ=255)
                                                           tGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGGTTTAATTTGTACCCTAAAAAAAACAAATCTACCAAAACAAAAAAAACGTTGGGTATGTAATTTTTAAAAATGAg                                                                               >  1:4277819/1‑140 (MQ=255)
                                                           tGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCGTTGAATTCTCCCGGAAAAAAAATAAAGATCCCCAAACAAAAAATACCGTGCGGGTTTATTTTTTTAAAAGTAg                                                                               >  1:5348076/1‑140 (MQ=255)
                                                                aataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTCATTTCTCCCGTAAAATAAATAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTGGtt                                                                         >  2:4276105/1‑140 (MQ=255)
                                                                aataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTACTTTTATTTGTCCCCGAAAATAAATAAAGCTCCCAAAACAACAAAGACGGGTGGGATGTATTTTTTTAATAgggggggtt                                                                          >  1:1528955/1‑133 (MQ=255)
                                                                 ataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTGATTTGTCCCGTAAAATAAAGAAAGCTCCCAATAAAACAAAAACGGGGCGGATTTATTTTTTAAAAAGGAGTGGTta                                                                        >  2:6323701/1‑140 (MQ=255)
                                                                 ataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCAATTCTCCCGTAAAATAAAGAAAGCTACCAATACAACAAATAAGGGGCGGATTTATTTTTTAAAAATGAGTCGGta                                                                        >  2:5127741/1‑140 (MQ=255)
                                                                 ataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTGATTTGTCCCCTAAAATAAATAAAGCTCCCCCAAAAACAAATACGGTGCGGAGTTATTTTTTTAAAAGGAGGCgggt                                                                        >  1:6419750/1‑137 (MQ=255)
                                                                 ataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTACTTTCATTTGTCCCGTAAAAAAAAGAAAGCTCCCCATACAAAAAAAACCGTGGGGATTTATTTTTTAAATAGGATTCTTta                                                                        >  2:6653842/1‑140 (MQ=255)
                                                                 ataaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGGCGTGTGGCTTTGCGTTCTCCCGGAAAAGAAAAAAAGCTCGCAATACAACAAATACCGGTGGGTGTTATATTTTGAAAAGGTGTCggtg                                                                        >  1:2558213/1‑137 (MQ=255)
                                                                  taaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCGTTGATTTTTCCCGTAAAAAAAAAAAAGCGACCAAAAAAAAAAAAAAAGGGCGGGTTTATTTTTTAAAAAATAGTGGTtat                                                                       >  1:49643/1‑140 (MQ=255)
                                                                  taaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTATCCCGTAAAATAAAGAAAGCTACCAAAACAACAAATACGGTGGGGATTTATTTTTTTAAAAGGAGGCGTtat                                                                       >  2:2703660/1‑140 (MQ=255)
                                                                  taaCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTACTTTCATTTATCCCGTAAAATAAAGAAAGCTCCCCATACAAAAAAAAAGGGGCGGGTTTATTTTTTAAAAATGAGTCGTtat                                                                       >  2:2477904/1‑140 (MQ=255)
                                                                    aCTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTatat                                                                     <  1:949562/140‑1 (MQ=255)
                                                                    aCTCCGTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTatat                                                                     <  2:2883895/140‑1 (MQ=255)
                                                                     cTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGGTTTCAGTTGTGCCGTAAAATAAATAAAGCTGCCCAAACAAACAATAAGGTTGGGCTTTATTTTTTAAAAACCAGTCggagtctt                                                                    >  1:2782668/1‑133 (MQ=255)
                                                                     cTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTGCTTTGATTTTTGCCGTAAAAAAAATAAAACTCCCAATAAAACAAAAACGGTGGGGCTTTTTTTTTTTAAAATGAGTGGtgttttt                                                                    >  1:6583177/1‑134 (MQ=255)
                                                                     cTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTCCTTTCATTTCTCCCGTAAAATAAAGAAATCTCCCAAAAAAACAAATACGGTGCGGATTTATTTTTTAAAAAGTAGTGGTtataat                                                                    >  2:690807/1‑138 (MQ=255)
                                                                       ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATAAAAAAAATACGGTTGGGATTTATTTTTTAAAAAGGAGTCGTTATATTTg                                                                  >  2:5197899/1‑140 (MQ=255)
                                                                       ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTGCTTTAATTTGTCCCGTTAAAAAAAAAAAGCTCACAAAACAACAAAAAAGGTGGGGATTTATTTTTTTAAAAGGATTCGTTATAtttt                                                                  >  2:1135849/1‑139 (MQ=255)
                                                                       ccTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTCCTTTCATTTATCCCGTAAAATAAAAAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTg                                                                  >  2:3011499/1‑140 (MQ=255)
                                                                          tGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGaat                                                               <  1:3353525/140‑1 (MQ=255)
                                                                          tGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACTTTCATTTCTCCCGTAAAATAAACAAACCTCCCCATACAACAAAAAACGTGCGGAGTTATTTTTTAAAAAGGAGTACTTGTATTTgtaa                                                               >  2:5939207/1‑137 (MQ=255)
                                                                           gCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGaata                                                              <  1:2471547/140‑1 (MQ=255)
                                                                                  aacaacATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATCCGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGttt                                                       <  1:6330131/140‑1 (MQ=255)
                                                                                  aacaacATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGttt                                                       <  1:5532173/140‑1 (MQ=255)
                                                                                  aacaacATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGttt                                                       <  1:3442835/140‑1 (MQ=255)
                                                                                       gctccTCATCGTGTCCTCTTTTTCTCGGGTCGTGTTCCTTTCTTTTCTCCCGTAAAATTAAGACAGCTCACAATACAACAACTTCGGTGCGGATTTATTTTTTAAAAAGGTGTCGTTATATTTGAATAATAGTTTTACAt                                                  <  2:7583207/136‑1 (MQ=255)
                                                                                       caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAAt                                                                                                                      <  1:1463955/74‑1 (MQ=255)
                                                                                       caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCAATTGTACCGTAAAATAAAGAAAGCGCCCCATACAACAAAAACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACAt                                                  >  2:6592508/1‑140 (MQ=255)
                                                                                       caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTACGTTGAATTCTACCCTAAAATAAAGAAAACACCCAAAACAAAAAAAAAGGGGCGGATTTATTTTTTAAAAAAGAGTCGTGAAATTTGAATAATAATATTACAt                                                  >  2:6267507/1‑140 (MQ=255)
                                                                                       caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTCCTTTCATTTCTCCCGTAAAAAAAAGAAATCTCCCAATAAAACAAAAAGGGTGCGGATTTATTTTTTAAAAAGGAGTGGGTATATTTTAATAATAGgttttaat                                                  >  2:4404202/1‑136 (MQ=255)
                                                                                       caTACTCAACGTGTCCTCTTTTTCTCCGGTCGTGTTCCATTCAATTCTCCCGTAAAATAAAAAAATCTCCCAAAACAAAAAATACGGTGCGGATTTATTTTTTATAAAGGAGTCGGTAAAATTTAAATAAATTTTTTAct                                                   >  2:6783002/1‑139 (MQ=255)
                                                                                                cGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAagcagc                                         <  1:4889925/140‑1 (MQ=255)
                                                                                                 gtgtCCTATTTTTCTCCGGTCGTGTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAgcagca                                        <  1:6747021/140‑1 (MQ=255)
                                                                                                 gggtCCTCTGTGTCTCCGGTCGTTTTCCTTTCATGTCTCCCCTAAAACAAAGAAAGCTCCCAATCCCACAAATACGGTGCGGAATTATTTTTTAAAAAGGAGACGTTATATTTGAATAATAGTTTTACATTAAAgcagca                                        <  1:4057805/138‑1 (MQ=255)
                                                                                                  tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                       <  2:6088213/140‑1 (MQ=255)
                                                                                                  tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                       <  1:7223935/140‑1 (MQ=255)
                                                                                                  tgtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                       <  1:4675570/140‑1 (MQ=255)
                                                                                                  ggtCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAAGACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCaa                                       <  2:2082982/139‑1 (MQ=255)
                                                                                                    tCCTCTTTTTCTCCGGTCGTGTTCCTTTGATTTCTGCCGGAAAATAAAGAAAGCTCCCAAAACAACAAATACGGTGCGGCTTTATTTTTTAAAAAGGATTCGTTATGTTTTAATAATATTTTTTAATTAAAGAAGCAATg                                     >  2:7679731/1‑140 (MQ=255)
                                                                                                      ctctTTTTCTCCGGTCGTTTTCCATTAATTTGTCCCGTAAAAAAAATAAAGCTCCCCAAAAAAAAAAAACGGTGGGGATTTATTTTTTAAAAAGGAGTGGGTATATTTTAATAATAGTTTTTCATTAAAGCAGCCATGGt                                   >  2:1277136/1‑140 (MQ=255)
                                                                                                       tctTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGtt                                  <  1:7198968/140‑1 (MQ=255)
                                                                                                        ctTTTTCTCCGGTCGTTTTCCTTTCATTTCTGCCGTAAAATAAAGAAAGCTCCCAATAAAAAAAATAAGGTGCGGATTTATTTTTTAAAAAGGGGTGGTTTTATTTTAATAATAGTTTTTGATTATATCATCAATGTTTc                                 >  2:7317769/1‑140 (MQ=255)
                                                                                                          ttttCTCCGGTCGTTTTGCTTTCATTTGTCCCGTAAAATAAAAAAACCTCCCAAAACAACAAATACGGTGGGGCTTTATTTTTTAAAAAGGAGTCGTTATATTTTTAAAATATTTTTTAATTAAAGAATCAAAGGTTggg                               >  2:4979868/1‑140 (MQ=255)
                                                                                                             tctcCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGaa                            <  2:1573937/140‑1 (MQ=255)
                                                                                                                   gTCGTTTTACATTAAATTCTACCGTAAAATAAAGAAAACTCCCAATACAACAAATAAGGTGCGGCTTTATTTTTTAAAAAGGAGTCGTTATATTTTAATAATATTTTTAACTTAAAGCAGCCATGGTGGGGGGATATACg                      >  2:3483850/1‑140 (MQ=255)
                                                                                                                              ttCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTc           >  2:3202845/1‑140 (MQ=255)
                                                                                                                              ttCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGGTATATTTGAATAATAGTTTTACATTAAAGCAGCACTGGTTCGGGACTATCCGAAAAGACAtta           >  2:4213795/1‑139 (MQ=255)
                                                                                                                                       cccGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCa  <  2:3354215/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
CCATTATTTTCTTCATCATAGATCTTGCGTATTTGATCAAAATTTAATATGATACTCTTTGTATAATAACTCCTTGCCTCAAAACAACATACTCAACGTGTCCTCTTTTTCTCCGGTCGTTTTCCTTTCATTTCTCCCGTAAAATAAAGAAAGCTCCCAATACAACAAATACGGTGCGGATTTATTTTTTAAAAAGGAGTCGTTATATTTGAATAATAGTTTTACATTAAAGCAGCAATGGTTCGGGAATATCCGAAAAGACATTCTTGCTGGCA  >  NZ_CP034943/176891‑177165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: