Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,901,667 T→A 9.0% Y410N (TAC→AAC)  EO946_RS14945 → extracellular solute‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,901,6670TA 9.0% 1009.9 / 14.8 327Y410N (TAC→AAC) EO946_RS14945extracellular solute‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (245/43);  new base A (2/28);  total (250/77)
Fisher's exact test for biased strand distribution p-value = 1.31e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 327 total aligned reads displayed.

ATGTCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGCTCTC  >  NZ_CP034943/2901533‑2901805
                                                                                                                                      |                                                                                                                                          
aTGTCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACaaa                                                                                                                                       >  1:3434683/1‑140 (MQ=255)
aTGTCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACaaa                                                                                                                                       >  2:4613103/1‑140 (MQ=255)
aTGTCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCGGGGAAGAGAATACaaa                                                                                                                                       >  2:2707743/1‑140 (MQ=255)
  gTCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACg                                                                                                                                     >  2:2613274/1‑140 (MQ=255)
   tCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGACGCTGGGACGAGAATACAAACGa                                                                                                                                    >  2:4772150/1‑140 (MQ=255)
   tCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGa                                                                                                                                    >  1:6563002/1‑140 (MQ=255)
   tCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGa                                                                                                                                    >  1:4049744/1‑140 (MQ=255)
   tCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGa                                                                                                                                    >  1:5501349/1‑140 (MQ=255)
   tCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGCCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATGTCGAAGAAGCTGGGAAGAGAATACAAACGa                                                                                                                                    >  2:3614413/1‑140 (MQ=255)
   tCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAAGGTGGGATGTCCTGGGCCCGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACgc                                                                                                                                    >  2:4029431/1‑139 (MQ=255)
     aaaGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGCTAATACAAACGACt                                                                                                                                  >  2:1844051/1‑140 (MQ=255)
                 tACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATc                                                                                                                      >  1:182130/1‑140 (MQ=255)
                   cTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCgg                                                                                                                    >  2:5703804/1‑140 (MQ=255)
                     gcgagcttttATCCTTGCCAACGGTATTGTGGGAGGGCCTGGGCCGGGTCGCACGGGTGTTTAGGGCTTAAATTTTGCCGCCTCAAGAGTTTTCCACCACGCGGGGAAGAAAATACCCACGACTCCGGGAGCAATCGGaa                                                                                                                  <  2:2224807/133‑1 (MQ=255)
                        aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCACTCGGAAaca                                                                                                               >  1:7164458/1‑140 (MQ=255)
                        aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAaca                                                                                                               >  2:1429172/1‑140 (MQ=255)
                        aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAaca                                                                                                               >  1:3719644/1‑140 (MQ=255)
                        aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAaca                                                                                                               >  2:4375951/1‑140 (MQ=255)
                        aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAaca                                                                                                               >  1:239509/1‑140 (MQ=255)
                        aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAaca                                                                                                               >  1:2140530/1‑140 (MQ=255)
                            tttATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAgg                                                                                                           >  2:4297068/1‑140 (MQ=255)
                            tttATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAgg                                                                                                           >  2:6996044/1‑140 (MQ=255)
                            tttATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAgg                                                                                                           >  1:5854133/1‑140 (MQ=255)
                             ttATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAggg                                                                                                          >  2:6688629/1‑140 (MQ=255)
                                     gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCACTCGGAAACACAGGGAGCTgaag                                                                                                  >  2:5462460/1‑140 (MQ=255)
                                     gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCACTCGGAAACACAGGGAGCTgaag                                                                                                  >  1:914405/1‑140 (MQ=255)
                                     gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                  >  1:3195869/1‑140 (MQ=255)
                                     gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                  >  1:2486460/1‑140 (MQ=255)
                                     gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                  >  1:5034412/1‑140 (MQ=255)
                                     gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                  >  2:2064520/1‑140 (MQ=255)
                                      ccAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCACTCGGAAACACAGGGAGCTgaaga                                                                                                 >  1:5418652/1‑140 (MQ=255)
                                      ccAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaaga                                                                                                 >  1:1159827/1‑140 (MQ=255)
                                      ccAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaaga                                                                                                 >  2:162398/1‑140 (MQ=255)
                                      ccAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaaga                                                                                                 >  1:269835/1‑140 (MQ=255)
                                           cGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGACACACAGGGAGCGGAAGAACaag                                                                                            >  2:6464495/1‑139 (MQ=255)
                                                    ggttGTCGTGGGCACGGGGGGACAGGAGGTTTTGGCTGAATTTTTGACGCATAAAGATATTTCGAAGAAGCTGGGAAGTTAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGAt                                                                                   <  2:7760294/137‑1 (MQ=255)
                                                        gTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCa                                                                               >  2:7177322/1‑140 (MQ=255)
                                                            tGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAagag                                                                           >  1:6868757/1‑140 (MQ=255)
                                                            tGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAagag                                                                           >  1:6287141/1‑140 (MQ=255)
                                                            tGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAagag                                                                           >  1:7757319/1‑140 (MQ=255)
                                                             ggcccgggTGGGACTGGTGTTTTTGGCTCATATTGTGCCGCAACAAGTTATTTCCAAGATGGTGGAAACAAAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAgaga                                                                          <  1:5208956/134‑1 (MQ=255)
                                                               gcccGGGCGCACAGGAGCTTCGGCCTGACTTTTTCCCCCCTCAACAGTTTTCCAAGCCGGGGGGAACACAACCCCCACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACg                                                                        <  1:5302899/137‑1 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGCGATTTCGAAGAAGCGGGGAAGTGAATACAAACGACTCCGGGAGCAATCGGAACCCCCGGGAGCTGAAGCACACTGAGTGAGATCGCAAGAGACGTTCa                                                                    >  1:7401426/1‑140 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGACTACAAACGACTCCGGGAGCCCTCGGAAACACAGGGAGCTGAAGACCAATGAGTGAGATCGCAAGAGACGTTCa                                                                    >  1:2902125/1‑140 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGACTACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                    >  2:289996/1‑140 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGCAACACAGGGAGCTGACGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                    >  1:6008840/1‑140 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                    >  1:2755297/1‑140 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                    >  2:7574402/1‑140 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                    >  1:4209983/1‑140 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                    >  2:4983643/1‑140 (MQ=255)
                                                                   ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                    >  1:1655614/1‑140 (MQ=255)
                                                                        gACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGa                                                               >  1:5256256/1‑140 (MQ=255)
                                                                        gACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGa                                                               >  1:2098250/1‑140 (MQ=255)
                                                                             gAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAg                                                          >  1:1253742/1‑140 (MQ=255)
                                                                                  tATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGaa                                                     >  1:4155837/1‑140 (MQ=255)
                                                                                   aTGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCACTCGGAAACACAGGGAGCTGAAGAACCATGAGTGAGATCGCACGAGACGTTCATGTGAAACAGGTGAAg                                                    >  2:4462710/1‑140 (MQ=255)
                                                                                   aTGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAg                                                    >  1:2965446/1‑140 (MQ=255)
                                                                                   aTGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAg                                                    >  1:3115281/1‑140 (MQ=255)
                                                                                   aTGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAg                                                    >  2:5278696/1‑140 (MQ=255)
                                                                                   aTGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAg                                                    >  2:6065348/1‑140 (MQ=255)
                                                                                   aTGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAg                                                    >  2:2505389/1‑140 (MQ=255)
                                                                                   aTGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAg                                                    >  2:7008416/1‑140 (MQ=255)
                                                                                       cttactttttGACGCCTCAATATTTTTCGAATACGGTGGTAAGATAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGa                                                <  2:7302514/134‑1 (MQ=255)
                                                                                       ctcaCATTTTGCCGCCTCACTCTATTACTAAGAAGCTGGTAATAGAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGa                                                <  2:6536220/137‑1 (MQ=255)
                                                                                       cgaaCATTTTCACGCCTCAAGATATTTCGATGACGGTGGTAATATAATAAACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGa                                                <  2:1345989/137‑1 (MQ=255)
                                                                                          aCATTTTGACGCGTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGaaaa                                             >  1:5945439/1‑140 (MQ=255)
                                                                                          aCATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGaaaa                                             >  2:3047319/1‑140 (MQ=255)
                                                                                          aCAGTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGACACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCAGGTGAAACAGGTGAAGCCGaaaa                                             >  1:2322649/1‑140 (MQ=255)
                                                                                            tttttGACGCATAAAGATTTTGCTAATATGCTGTTAATATAAAACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAg                                           <  2:4706404/139‑1 (MQ=255)
                                                                                            agtgtcaCGCCTCAAGACATTCCTAAGATGGTTGTACTACAAAACCCACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAg                                           <  1:5703804/134‑1 (MQ=255)
                                                                                             ttttCACGCCTCAACAGTTTTCCACTACGCTGGTAACACAAAACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGc                                          <  1:1429172/140‑1 (MQ=255)
                                                                                               ttGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAg                                        >  2:3294751/1‑140 (MQ=255)
                                                                                                tGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGt                                       >  2:3812949/1‑140 (MQ=255)
                                                                                                      cTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCAc                                 >  1:4350789/1‑140 (MQ=255)
                                                                                                      cTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCAc                                 >  1:6488135/1‑140 (MQ=255)
                                                                                                      cTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCAc                                 >  1:6424991/1‑140 (MQ=255)
                                                                                                        cAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTTAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACtt                               >  2:5697357/1‑140 (MQ=255)
                                                                                                        cAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACtt                               >  2:355189/1‑140 (MQ=255)
                                                                                                           cagaTTTCCAACATGGGGGTAAGACAACACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtgg                            <  2:413383/139‑1 (MQ=255)
                                                                                                              aTTTCTAATAAGCTGGTAACACAAAACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtggtgg                         <  2:5546094/140‑1 (MQ=255)
                                                                                                              aTTCCGAACACGGTGGGAATACAAAACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtggtgg                         <  1:1040958/140‑1 (MQ=255)
                                                                                                                ttCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                       >  2:293292/1‑140 (MQ=255)
                                                                                                                ttCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                       >  2:7395796/1‑140 (MQ=255)
                                                                                                                ttCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                       >  2:4814202/1‑140 (MQ=255)
                                                                                                                ttCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                       >  2:5158307/1‑140 (MQ=255)
                                                                                                                ttCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                       >  2:4926300/1‑140 (MQ=255)
                                                                                                                ttCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGATCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCCCTTTGTTGGAt                       >  2:5858643/1‑140 (MQ=255)
                                                                                                                ttCCAACAAGGTGTGAATACAACACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                       <  2:6804801/140‑1 (MQ=255)
                                                                                                                 tCGAATAAGCTGGTAAGATAATACCAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATg                      <  2:5226045/140‑1 (MQ=255)
                                                                                                                   gaagaaGCTGGGATGATAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGta                    <  2:3758916/140‑1 (MQ=255)
                                                                                                                           tGGTAAGATAATAAACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTg            <  2:2107153/140‑1 (MQ=255)
                                                                                                                           tGGGAAGATAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTg            <  2:2322649/140‑1 (MQ=255)
                                                                                                                           tGGGAAGACAATCCACCCGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTg            <  2:3138038/140‑1 (MQ=255)
                                                                                                                               atcagaATAAACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCttt        <  1:4801155/137‑1 (MQ=255)
                                                                                                                                  ataaTAAACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGct     <  2:1189359/138‑1 (MQ=255)
                                                                                                                                    aaTACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct   <  2:6295270/140‑1 (MQ=255)
                                                                                                                                    aaTACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct   <  2:1704626/140‑1 (MQ=255)
                                                                                                                                    aaTACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct   >  1:972192/1‑140 (MQ=255)
                                                                                                                                    aaTACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct   <  2:5955195/140‑1 (MQ=255)
                                                                                                                                    aaTACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAAGGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct   >  2:198065/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                          
ATGTCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGCTCTC  >  NZ_CP034943/2901533‑2901805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: