BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,910,798	C→A	5.7%	M1M (ATG→ATT) †	EO946_RS14985 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,910,798	0	C	A	5.7%	985.4 / 8.4	355	M1M (ATG→ATT) †	EO946_RS14985	MFS transporter
Reads supporting (aligned to +/- strand): ref base C (202/120); new base A (4/28); total (207/148)
Fisher's exact test for biased strand distribution p-value = 3.99e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 361 total aligned reads displayed.

CAGCATCAGCACAAGTCCGGCTACTGTTAATGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAAT  >  NZ_CP034943/2910660‑2910937

cagcatcagcaCAAGTCCGGCTACTGTTAATGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCa                                                                                                                                            <  1:829400/140‑1 (MQ=255)

cagcatcagcaCAAGTCCGGCTACTGTTAATGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCa                                                                                                                                            <  1:1742701/140‑1 (MQ=255)

cagcatcagcaCAAGTCCGGCTACTGTTAATGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCa                                                                                                                                            <  1:5553986/140‑1 (MQ=255)

cagcatcagcaCAAGTCCGGCTACTGTTAATGACTTTCCTAAAGGATTATGGATATAAATCGTATTCAGCGGCCATAAACAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCa                                                                                                                                            <  1:2659981/140‑1 (MQ=255)

      cagcaCAAGTCCGGCTACTGTTAATGACTTTCCTAAATGATTATGGATATACATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGAGGCATACTaa                                                                                                                                      >  2:7215672/1‑140 (MQ=255)

        gcaCAAGTCCGGCTACTGTTAATGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATc                                                                                                                                    <  1:1003753/140‑1 (MQ=255)

             aGTCCGGCTACTGTTAATGACTTTCCGAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATCCTAATCCCCCt                                                                                                                               >  1:981668/1‑140 (MQ=255)

              gTCCGGCTACTGTTAATGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATc                                                                                                                                    <  2:5316355/134‑1 (MQ=255)

              gTCCGGCTACTGTTAATGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATc                                                                                                                                    >  1:5316355/1‑134 (MQ=255)

                 cGGCTACTGTTAATGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTttct                                                                                                                           >  1:4020471/1‑140 (MQ=255)

                 cGGCTACTGTTAATGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTttct                                                                                                                           >  1:1263195/1‑140 (MQ=255)

                  ggCTACTGTTAATGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTttctt                                                                                                                          <  2:1566643/140‑1 (MQ=255)

                   gCTACTGTTAATGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTttcttc                                                                                                                         >  1:5639657/1‑140 (MQ=255)

                   gCTACTGTTAATGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTttcttc                                                                                                                         >  1:1625242/1‑140 (MQ=255)

                   gCTACTGTTAATGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTttcttc                                                                                                                         >  1:4931838/1‑140 (MQ=255)

                   gCTACTGTTAATGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTttcttc                                                                                                                         >  1:3364618/1‑140 (MQ=255)

                   gCTACTGTTAATGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCACCCTttcttc                                                                                                                         >  2:5701904/1‑140 (MQ=255)

                      aCTGTTAATGACTTTCCTAAATGATTATGGATATAAATCGTAGTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTCATCCCCCTTTCTTCCCt                                                                                                                      >  2:61480/1‑140 (MQ=255)

                        tGTTAATGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTaa                                                                                                                    >  1:6296097/1‑140 (MQ=255)

                        tGTTAATGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTaa                                                                                                                    >  1:7723470/1‑140 (MQ=255)

                        tGTTAATGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTaa                                                                                                                    >  1:441017/1‑140 (MQ=255)

                            aaTGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTaaaaaa                                                                                                                >  1:1108687/1‑140 (MQ=255)

                            aaTGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCAGAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTaaaaaa                                                                                                                >  1:1231545/1‑140 (MQ=255)

                              tGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGc                                                                                                              >  2:47770/1‑140 (MQ=255)

                              tGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGc                                                                                                              >  2:2625327/1‑140 (MQ=255)

                              tGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGc                                                                                                              >  2:6411485/1‑140 (MQ=255)

                                  tttCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCAt                                                                                                          >  1:2798238/1‑140 (MQ=255)

                                  tttCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCAt                                                                                                          >  2:2831690/1‑140 (MQ=255)

                                  tttCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCAt                                                                                                          >  2:5945144/1‑140 (MQ=255)

                                  tttCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCAt                                                                                                          >  1:2145315/1‑140 (MQ=255)

                                  tttCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGAACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCAt                                                                                                          >  2:4901937/1‑140 (MQ=255)

                                  tttCCTAAATGATTATGGATATAAATCGTATTCAGAGGCCATAAAAAAGAAGCAACCGTCACATTGATAAACATTCAAATGATTAAAATTTTAAGAGCACGCGGAATACTAAAACCCCTTTCTTCCAAAAAAAAGATCAt                                                                                                          >  2:3788259/1‑140 (MQ=255)

                                    tCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGa                                                                                                        >  1:5944712/1‑140 (MQ=255)

                                    tCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGa                                                                                                        >  2:6956856/1‑140 (MQ=255)

                                    tCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGa                                                                                                        >  2:3400800/1‑140 (MQ=255)

                                             aTTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGa                                                                                               <  1:205200/140‑1 (MQ=255)

                                             aTTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGa                                                                                               >  1:5188349/1‑140 (MQ=255)

                                               tATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACTaaa                                                                                             >  2:4057864/1‑140 (MQ=255)

                                                aTGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACTaaaa                                                                                            >  2:4307151/1‑139 (MQ=255)

                                                  gtatatAAATCTTATTCAGCGGCAATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCGTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCt                                                                                          <  2:2988257/138‑1 (MQ=255)

                                                     tataAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTaaa                                                                                       >  2:3128634/1‑140 (MQ=255)

                                                      ataAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTaaaa                                                                                      >  1:7489870/1‑140 (MQ=255)

                                                      ataAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACAGTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGATCATGAAAAAAACGAAACCTaaaa                                                                                      >  2:3738508/1‑140 (MQ=255)

                                                        aaaTCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAAc                                                                                    >  2:3254102/1‑140 (MQ=255)

                                                           tcttATTCAGCGGCAATAAAAAATAATAACCCGTCACATTGATAAACTTTCAAATTATTAAAATTTTAAGCGCACGCGGAATACTAATCCCCATTTCTTCCCTAAAAAAGCTCATTAAAAAAACGAAACCTAAAAACGGc                                                                                 <  2:1263204/137‑1 (MQ=255)

                                                              tATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTcc                                                                              >  2:361020/1‑140 (MQ=255)

                                                              tATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTcc                                                                              >  2:6813450/1‑140 (MQ=255)

                                                              tATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTcc                                                                              >  2:842754/1‑140 (MQ=255)

                                                              tATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTcc                                                                              >  1:4318898/1‑140 (MQ=255)

                                                              tATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCAAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTCAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTcc                                                                              >  1:2750806/1‑140 (MQ=255)

                                                                     cGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGaa                                                                       >  1:6974978/1‑140 (MQ=255)

                                                                     cGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTACAATGATTAAAATTTTAATAGCACGCTGCATACTAATCCCCCTTTCTTCCCTAAAAAATCTAATGAAAAAAAATAAACCTAAAAACGGCTCCGTACTaa                                                                       >  1:4512825/1‑140 (MQ=255)

                                                                      ggCCATAAAAAAGAACCACCCGTCAAATTTATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATTAAAAAAACGAACCCTAAAAACGGCTCCGTACGAAt                                                                      <  2:1108687/140‑1 (MQ=255)

                                                                      ggCCATAAAAAACAACCACCCCTCAAAATCATAAACATTCCAATGATTAAAATTTTACGCGCACGCGGCATACAAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAAt                                                                      <  2:5182912/140‑1 (MQ=255)

                                                                      ggCAATAAAAAATAAGCCCCCGTCACATTGATAAACATTCAAATTATTAAATTTTTAAGAGCACGCGGAATAATAATCCCCCTTTCTCCCCTAAAAAAGCTAATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAAt                                                                      <  1:3661769/140‑1 (MQ=255)

                                                                      ggCAATAAAAAATAACCACCCGTAAATTTTATAAAAATTCAAATTATTAAAATTTTAAGAGCCCGCGGAATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAACACGGCTCCGTACGAAt                                                                      <  1:1509060/140‑1 (MQ=255)

                                                                      ggCAATAAAAAAGAACCACCCCTCACATTTATAAACTTTCCAATTATTAAAATTTTAAGAGCACGCGGAATACTAAACCCCCTTTCTTCCCTAAAAAACCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAAt                                                                      <  1:5725481/140‑1 (MQ=255)

                                                                         cATAAAAAACAACCACCCGCCACTTTTATAAACATACAAATCATTAAAATTTTACGAGCCCGCGGAATACTAACCCCCCTTTCGTCCCAAAAAACGCTCATCAAAAAAACCAAACCTAAACACGGCTCCGTACGAATCGa                                                                   <  1:2342320/140‑1 (MQ=255)

                                                                          aTAAAAAAGAAGCACCCGTAACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAg                                                                  <  2:7517123/140‑1 (MQ=255)

                                                                           tAAAAAAGACGCACCCTTCACATTTATAAACATTCCAATTATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGt                                                                 <  2:6280225/140‑1 (MQ=255)

                                                                           tAAAAAAGAAGCACCCTTCACATTTATAAACATTCCAATTATTAAATTTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAACCCGGCTCCGTACGAATCGAGt                                                                 <  2:6848925/140‑1 (MQ=255)

                                                                           tAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAACCCTAAAAACGGCTCCGTACGAATCGAGt                                                                 <  2:2144868/140‑1 (MQ=255)

                                                                           tAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGt                                                                 <  2:656444/140‑1 (MQ=255)

                                                                           aaaaaaaCAACCACCCGCCAAATTGAAAAACATACCAATGATTAAAATTTTAAGCCCCCGCGGCAAACAAAGCCCCCTTTCTCCCCTAAAAAAGCAAATCAAAAAAACCAAACCAACAAACGGCTCCGTACGAATCGAGt                                                                 <  1:3577422/139‑1 (MQ=255)

                                                                              aaaaTAAGCCCCCGTAAAATTGAAAAACATACAAATTATTAAATTTTTACGCGCACGCGGAATACTAATCCCCCTTTCTCCCCAAAAAAAGCTCATTAAAAAAACGAAACCAAAACACGGCTCCGTACGAATCGAGTTAt                                                              <  1:3013265/140‑1 (MQ=255)

                                                                              aaaaTAAGCACCCGTAACATTGATAAACATACAAATTATTAAATTTTTAAGAGCCCGCGGAATAATAATCCCCCTTTCTCCCCAAAAAAAGATCATTAAAAAAACGAAACCTAAACACGGCTCCGTACGAATCGAGTTAt                                                              <  1:4819718/140‑1 (MQ=255)

                                                                               aaaGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATc                                                             <  2:5270066/140‑1 (MQ=255)

                                                                                   aagCACCCTTCATATTTATAAACATTCCAATTATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCGCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAg                                                         <  2:6746722/140‑1 (MQ=255)

                                                                                   aagCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAg                                                         <  2:1625242/140‑1 (MQ=255)

                                                                                   aagCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAg                                                         <  2:441017/140‑1 (MQ=255)

                                                                                   aagCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAg                                                         <  2:7671372/140‑1 (MQ=255)

                                                                                       accctaaacaTTGAAAAACATTCAAATGATAAAAATTTTAAGACCACGCGTAATAATAATCCCCCTTTCTCCCCAAAAAACGCTCATTAAAAAAACCAAACCTAAACACGGCTCCGTACGAATCGAGTTATCATAGcaca                                                     <  1:4073952/133‑1 (MQ=255)

                                                                                       aCCCGTCACTTTTATAAACATTCCAATGATTAAAATTTTAAGCGCACGCGGCATAATAAACCCCCTTACTTCCCTAAAAAAGCTCATCAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGcaca                                                     <  1:3794126/140‑1 (MQ=255)

                                                                                         ccGTCACCTTGATAAACATTCCACTGATTAAAATTTTAAGAGCACGCGGCTTACTAATCCCCCTTTGTTCCCTAAAAAACCTCATGAAAAAAACCAACCCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCAcatt                                                   <  2:1679528/140‑1 (MQ=255)

                                                                                         ccGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCAcatt                                                   >  2:5798122/1‑140 (MQ=255)

                                                                                         ccGTCACATTGAAAAACATTCCAATGATTAAAATTTTAAGACCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTAATCAAAAAAACCAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCAcatt                                                   <  1:7597604/140‑1 (MQ=255)

                                                                                            tCACATTTATAAACATTCCAATGATTAAAATTTAAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATCAAAAAAACGAAACCTAAACACGGCTCCGTACGAATCGAGTTATCATAGCAcattcat                                                <  1:7334238/140‑1 (MQ=255)

                                                                                             cacaTTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAACACGGCTCCGTACGAATCGAGTTATCATAGCAcattcatt                                               <  2:1487220/140‑1 (MQ=255)

                                                                                                     tAAACATTCCAATCATAAAAATTTTACGAGCCCGGGGATAACTAACCCCCGTTTCTTCCCTAAAAAACCTAATGAAAAAAACGAACCCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTAtctct                                       <  1:6528047/140‑1 (MQ=255)

                                                                                                      aaaCATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTc                                                                               <  2:1930685/99‑1 (MQ=255)

                                                                                                      aaaCATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTc                                                                               >  1:1930685/1‑99 (MQ=255)

                                                                                                          aTTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGtc                                  >  2:1385569/1‑140 (MQ=255)

                                                                                                            tCCACTGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGtctc                                >  1:1424253/1‑140 (MQ=255)

                                                                                                            tCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGtctc                                >  2:2406741/1‑140 (MQ=255)

                                                                                                            tCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGtctc                                >  1:929873/1‑140 (MQ=255)

                                                                                                            tCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGtctc                                >  2:1768893/1‑140 (MQ=255)

                                                                                                            tCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAAACGAGTTATAATATCACATTCAATCTATCTCTCTGtctc                                >  1:6731264/1‑140 (MQ=255)

                                                                                                            tCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAAAGGCTCCGTACGAATAGAGTTATCATAGAACATTCATTCTATCTCTCTGtctc                                >  2:6630119/1‑140 (MQ=255)

                                                                                                            tCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACATAAAAACTGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGtctc                                >  2:3535101/1‑140 (MQ=255)

                                                                                                            tCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCATTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATAGAGTTATCATAGCACATTCATACAATCTCTCTTtctc                                >  2:5541304/1‑140 (MQ=255)

                                                                                                              cAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTcac                              >  2:1811352/1‑140 (MQ=255)

                                                                                                              cAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTAAGAATCGAGTTATCATAGCACATGCATTCTATCTCTCTGTCTcac                              >  2:2262658/1‑140 (MQ=255)

                                                                                                                  tataaaaaTTTTAAGACCCCGCGCAATACTAACCCCCCTTTCTGCCATAAAAAAGCTCATCAAAAAAACCAACCCTAAACACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACAttt                          <  1:3128634/136‑1 (MQ=255)

                                                                                                                  taTTAAAATTTAAACACCACGCGGAATACTAAACCCCATTTCTCCCCAAAAAAAGCAAATCAAAAAAACCAAACCAAAACACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACAttt                          <  1:146223/139‑1 (MQ=255)

                                                                                                                    ttAAAATTTTAAGCGCCCGCGCCATACTAAGCCCCCTTTCTCCCATAAAAAAGCTAATTAAAAAAACCAAACATAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACAttttt                        <  1:6185518/140‑1 (MQ=255)

                                                                                                                       aaaTTTTACTAGCACGCGCCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACCAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCaa                     <  2:1451183/140‑1 (MQ=255)

                                                                                                                             tAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACtgatg               >  1:2560395/1‑140 (MQ=255)

                                                                                                                                      gcgGCATAATAATCCCCCTTTCTTCCCTAAAACAGCTCATGAAAAAAACTAACCCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGaaa      <  2:4589281/140‑1 (MQ=255)

                                                                                                                                          cATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAAt  <  2:1947021/140‑1 (MQ=255)

                                                                                                                                          cATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAAt  <  2:7489870/140‑1 (MQ=255)

CAGCATCAGCACAAGTCCGGCTACTGTTAATGACTTTCCTAAATGATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAAT  >  NZ_CP034943/2910660‑2910937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`