Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,979,628 T→A 12.6% noncoding (1173/2928 nt) EO946_RS15430 ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,979,6280TA12.6% 0.4 / 5.7 39noncoding (1173/2928 nt)EO946_RS1543023S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (6/28);  new base A (2/3);  total (8/31)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTACTCATACCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACCACTGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCAACATCCTGGTTGTCTAAGCAACTCCACATCCTTTTCCACTTAACGTATACTTTGGGACCTT  >  NZ_CP034943/2979490‑2979755
                                                                                                                                          |                                                                                                                               
cgatACTCATACCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACCACTGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTTTAGCCCCGGTACAtttt                                                                                                                                <  1:4233828/137‑1 (MQ=1)
               cagtctcACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTCCCACTGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACt                                                                                                                 <  2:4575570/137‑1 (MQ=1)
               cagtctcACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACCACTGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACt                                                                                                                 <  2:3843448/137‑1 (MQ=1)
                     caattCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACCACTGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCa                                                                                                           <  2:4775786/137‑1 (MQ=1)
                        ttgtAAGCGCGCCACCAGTCCGTCCGGTCTGGCGTACCAGCCCTTAGAACGCTCTCCTACCACTGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTg                                                                                                        <  2:3058844/137‑1 (MQ=1)
                        ttatAAGCGCTCCAACAGTGCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACCACTGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTg                                                                                                        <  2:5465909/137‑1 (MQ=0)
                                  tcgaccaGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACCACTGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTATCGGCGCAGAGTCTCTCGACCAGTGAGCTATTACg                                                                                              <  1:4042928/137‑1 (MQ=0)
                                    catcaGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACCACTGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCa                                                                                            <  2:2930580/137‑1 (MQ=1)
                                          tcattCCGGTCTGGCTTCACAGCCCTTACAACGATCTCCTACCACTGTTCGAACAACAGTCCGCAGCTTCGCTGATACGTTTAGCCCCGGTACATTAACGGCCCAGAGTCACTCGACCAGTGAGCTATTACGCActcgat                                                                                      >  2:4414257/4‑137 (MQ=1)
                                              tctggTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACCACTGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCCGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAAt                                                                                  >  2:32634/4‑140 (MQ=1)
                                              tctggTCTGGCTTCACAGCCCTTAGAAAGCTCTCCTACCACTGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACTCACTCTTTAAAt                                                                                  >  2:32507/4‑140 (MQ=0)
                                                   tcgggCGTCACAGCCCTTATAACGCTCTCCTACCACGGTTCGAAGAACAGTCCGCCGCTTCGGTGATACGTTTAGCCCCGGTACATTATCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAAtggtgg                                                                             <  2:6683248/137‑1 (MQ=1)
                                                    atgtcTTCACAGCACTTAGAACGGTCTCCTACCAATGTTCGACGATCAGTCCGCAGCTTCGGTGATAAGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGgc                                                                            <  1:1666676/136‑1 (MQ=1)
                                                            accgCCGTTAGGACGCTCTCCGACCCCTGTTCGATGAACAGTCCGCAGCTTCGCTGCTACGTTTAGCCCCGGTACATTTTCGGCGCAGAGGCGCTCGCCCTGTGAGCTCGTACGCCCTGTGGAACTGGTGGGTGCATCTa                                                                    >  2:4454373/4‑140 (MQ=0)
                                                            accgCCCTTAGAACGCTCTCCTACCACTGTTCGATGAACAGTCCGCAGCTTCGGGTATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGTCCAGTGAGCTATTACGCACTCTTTAAATGGTGTCTGCTTCtt                                                                    <  2:6698859/137‑2 (MQ=1)
                                                            accgCCCTTAGAACGCTCTCCTACCACTGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTATCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTa                                                                    <  1:2078327/137‑1 (MQ=1)
                                                                 ccgtAGAACGCTCTCCTACCACTGTTCGAAGAACAGTCCGCCGCGTCGGTGATACGTTTAGCCGCGGTACATTTTCGGCGCTGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCa                                                               <  2:6972606/137‑1 (MQ=1)
                                                                 ccctAGACCGCTCTCCTCCCCCCGTTCGAAGAACAGTCCGCAGCTTCGGTGAAACGTTTAGCCCCGGGACCCTTTCCGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCa                                                               <  2:4532292/137‑1 (MQ=1)
                                                                 ccatAGAACGCTCTCCTACCACTGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTTGAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCa                                                               <  2:6278136/137‑1 (MQ=1)
                                                                  ctgaGAACGCTCTCCTACCACTGTTCGAAGAACAGGCCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCaa                                                              <  2:7311970/137‑1 (MQ=1)
                                                                              tcatACCACTGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCAACATCCTGGTTGt                                                  <  2:2519847/137‑1 (MQ=1)
                                                                               ccgaCCACTGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCAACATCCTGGTTGTc                                                 <  2:5245146/137‑1 (MQ=1)
                                                                                    cagtGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGTGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCAACATCCTGGTTGTCTAAGc                                            <  1:3432986/137‑1 (MQ=1)
                                                                                     acggtgcGAAGACCAGTCCGCAGCGTCGGGGATACGTTGAGCCCCGGTACAGTTTCGGCGCAGAGTCACTCGTCCAGTGAGCTAGTACGCACACTTTAAATGGTGGCTGCGTCTAAGCCAACATCCTTGTTGTCTAAGCa                                           <  2:972676/134‑1 (MQ=1)
                                                                                     acggTTCGAAGAACCGTCCGCAGCTTCGGTGATACGGTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCAACATCCTGGTTGTCTAAGCa                                           <  2:1235968/137‑1 (MQ=0)
                                                                                      cttttCGAAGAACAGTCCGCAGCTTCTGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCAACATCCTGGTTGTCTAAGCaa                                          >  2:6387785/4‑140 (MQ=1)
                                                                                        gtgcGAAGAACAGTCCGCAGCTTCGGTGATACGTTTCGCCCCTGTACATTATCGGCGCAGAGTCACTCGACCCGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCAACATCCTGGTTGTCTAAGCAACt                                        >  2:2132067/4‑140 (MQ=1)
                                                                                          tctaagaaCAGTCCGCAGCTTCGGTGATTCGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAc                                                                                           <  1:3670912/84‑1 (MQ=0)
                                                                                          tctaagaaCAGTCCGCAGCTTCGGTGATACTTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCCGTGAGCTATTAGGAACTCTTTAAATGGTGGCTGCTTCTAAGCCAACAGCCTGGTTGTCTAAGCAACTcc                                      >  2:3983811/4‑140 (MQ=1)
                                                                                          tctaagaaCAGTCCGCAGCTTCGGTGATACGTGTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCAACATCCTGGTTGTCTAAGCAACTcc                                      <  2:3974187/137‑1 (MQ=1)
                                                                                             aataaCAGTCCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCAACATCCTGGTTGTCTAAGCAACTCcaca                                   <  1:1302005/137‑1 (MQ=1)
                                                                                             aacaaCAGTCCGCAGCGTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGAACTCTTAAAATGGTGGCTGCTTCTAAGCCAACATCCTGGTTGTCTAAGCAACTCcaca                                   <  1:3142034/137‑1 (MQ=0)
                                                                                                   agtcaccAGCTTCGGTGATAAGTTTATAAAAGGAACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCAACATCCTGGTTGTCTAAGCAACTCCACATCCttt                             >  2:5433866/7‑140 (MQ=1)
                                                                                                        gctgCTTCGGTGATACGTTTAGCCCCGGTAGATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAGTCTTTAAATGGTGGCTGCTTCTAAGGCAACATCCTGGTTGTCTAAGCAACTCCACATCCTTTTCCAc                        <  2:3822017/137‑1 (MQ=0)
                                                                                                        gccgCTTCGGTGATCCGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCAACATCCTGGTTGTCTAAGCAACTCCACATCCTGTTCCAc                        <  2:3668324/137‑1 (MQ=0)
                                                                                                        gccgCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCAACATCCTGGTTGTCTAAGCAACTCCACATCCTTTTCCAc                        <  2:1498618/137‑1 (MQ=1)
                                                                                                        gccgCTTCGGTGATAAGTTTAGCCCCGGTTCATTTTCGGCGCATAGTCACTCGACCCGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCAACATCCTGGTTGTCTAAGCAACTCCACATCCTTTTCCAc                        <  1:3475169/137‑1 (MQ=1)
                                                                                                                ggggacaCGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTAGTACGCACTCTTTAAAGGGTGGCTGCTTCTAAGCCAACATCCTGGTTGTCTAAGCAACTCCACATCCTTTTCCACTTAACGta                <  1:4614980/134‑1 (MQ=0)
                                                                                                                            agacccGGTACTTGTTCGGCGCAGAGTCACTCGCCCAGTGAGCTATTACGCCCTCTTTAAATGGTGGCTGCTTCTAACCCACCATCCGGGTAGTCTAAGCAACTCCACATCCTATTCCACGTAACGTATACTTTGGGAcc    <  2:5758627/137‑1 (MQ=1)
                                                                                                                              cccagttACCTTTTCGGCTGAGAGTGAATAGTACAGTGATCTATTACGCACTATTTAATTGTTGGCTTCTTCTAAGCCTACTTGCTGTTTGTCTATTCAACTCCAAATCATTTTCCAATTAACGTATACTTTGGGACCtt  <  2:1064904/134‑1 (MQ=0)
                                                                                                                                          |                                                                                                                               
CGCTACTCATACCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACCACTGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCAACATCCTGGTTGTCTAAGCAACTCCACATCCTTTTCCACTTAACGTATACTTTGGGACCTT  >  NZ_CP034943/2979490‑2979755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑