Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,980,160 G→A 21.0% noncoding (641/2928 nt) EO946_RS15430 ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,980,1600GA21.0% 1.2 / 18.4 45noncoding (641/2928 nt)EO946_RS1543023S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (12/23);  new base A (3/6);  total (16/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

AGTGTTACCTGAACTTCACCCTGGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTA  >  NZ_CP034943/2980034‑2980295
                                                                                                                              |                                                                                                                                       
agcgtTACCTGCACTTCACCCTGGACATGGGGAGATCACCTGGTTTCGGCTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACAGGATCGTAACTCg                                                                                                                            <  1:4269579/137‑1 (MQ=1)
     tagcTGAACTTCACCCTGGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACAGGATCGTAACTCGCCggg                                                                                                                       >  1:5706393/4‑139 (MQ=0)
        ctcaaCTTCCCCCTGGACACCGCTAGCTCACCCGGTTTCGGGACTACGACCACGTACTCATGCGCCCTAGTCAGACTCGCTTGCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACAGGCTCGTAACTCGCCGGTTCa                                                                                                                    <  2:7568103/137‑1 (MQ=1)
          gaccTTCACCCTGGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACACGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCAtt                                                                                                                  <  2:370839/137‑1 (MQ=1)
                caaccgggACATGCGTAGAACAACTGGTTTCGGGTCTACTACCACTTACTCATGCGCCCTATTCAGACTAGCTTTCGCTTCGGCTCCACATCGACAGCTTAACCTTGCACCGGATCCTAACTCGCCGGATCATTCTACaa                                                                                                            >  2:5808384/7‑140 (MQ=1)
                             gttacaTCACCTGGTTTCGGGTCTAATAAAACATACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATc                                                                                               >  2:1670160/6‑140 (MQ=1)
                             gttacaTCACCTGGTTTATTGTCTACTAACAAGAACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATc                                                                                               >  2:4305089/6‑140 (MQ=1)
                             gggaGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATc                                                                                               <  2:6369924/137‑1 (MQ=1)
                             ggaaGATCACCTGGTTTCGGGTCTACGAACACCTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCGTAACCTAGCACAGGAACGTAAATCGCCGGTTCATTCTACAAAATGCACGCCATc                                                                                               >  1:5158661/4‑140 (MQ=1)
                                 gaccACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCAccc                                                                                           >  1:3772560/4‑140 (MQ=1)
                                       cttgTTTAGGGTCTACGACCAATTAAAAATGAGACCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACAGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAAc                                                                                     >  2:4593424/4‑140 (MQ=1)
                                          gtgtCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACggg                                                                                  <  1:3851625/137‑1 (MQ=1)
                                          gtgtCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACggg                                                                                  <  2:3627940/137‑1 (MQ=1)
                                          gtgtCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACggg                                                                                  <  1:6598799/137‑1 (MQ=1)
                                                 gtatACGACCACGTTCTCATGCGCCCTATTCAGACTCTCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCGTGCACGGGATCGGAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGac                                                                           <  1:2791531/137‑1 (MQ=1)
                                                 gtatACGACCACGTACTCATGCGCCCTATTCAGTCTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGac                                                                           <  2:2804861/137‑1 (MQ=1)
                                                 gtatACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGac                                                                           <  2:4360747/137‑1 (MQ=1)
                                                 gtatACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGCCACGCCATCACCCGTTAACGGGCTCTGac                                                                           <  1:428150/137‑1 (MQ=1)
                                                 ggctccGACCACGGTCGCATGCGCCCGAGTCCGACCCGCGTTCGCTGCGGCTCCCCATCGTCTGCTTAACCGTGCACGGGATCGTAACTCGCCGGTTCATTCTCCCAAAGGCACGCCATCACCCGTTAACGGGCTCGGac                                                                           <  2:2245566/135‑1 (MQ=1)
                                                            cgaaCTAATGCGCCCTATTCAGACTAGCTTTCGCTGAGGCTCCGCCTCTTCTGCTTAACATTGCACGGGATCGGAACTCGCAGGATCAATCTACAAAATGCACGCCATCACCCGGTAACGGGCTCTGACTACTTGTAGGc                                                                >  2:2559529/4‑140 (MQ=1)
                                                               tctgaTGCGCCATTTTCAGACTCGCTTTAGATGCTGCTACGAATATTCTGATTAACATTGCAAGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGACTTCACCCGTTAACGGGCTCTGACTACTTGTAGGcaca                                                             <  1:1659567/136‑1 (MQ=1)
                                                               ccgcaagcgcCCTAGTCAGACTCGCGTTCGCTGCGGCTCCGCATCTTATGCTTAACCTGGCACGGGATCGTCACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGcaca                                                             <  2:2079845/134‑1 (MQ=1)
                                                               acgcATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCCGCTTCACCTTGCACGGGATCGGAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGcaca                                                             <  1:5968683/137‑1 (MQ=0)
                                                               acgcATGCGCCCTATTCAGACTCGCGTTCGCGGCGGCTCCGCATCTTCTGCTTAACCTTGCACAGGATCGTAACTCGCCGGGTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGcaca                                                             <  1:6171517/137‑1 (MQ=1)
                                                                           tagtCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACAGGATCGGACCTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGAt                                                 <  1:6361389/137‑1 (MQ=0)
                                                                           taatCAGACTCTCTTTAGAAGCGGCACCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGGGCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGTCTACTTGTAGGCACACGGTTTCAGGAt                                                 >  1:3397146/4‑140 (MQ=1)
                                                                                  acacGCTTTCGCTGCGGCTCCGCATATTCTGCTTAACCTTGCAAGGGAACGTAACTCACCGCTTCATTCTAAAAAAGGCACGCAATCAACCGTTAACGGGCTCTGACTAATAGTAGGCACACGGGTTCAGGATCACttta                                          >  2:7031339/4‑139 (MQ=1)
                                                                                  aatcccTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGGTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCGGACTACTTGTAGGCACACGGTTGCAGGATGTCTTTc                                          >  2:5973783/6‑140 (MQ=1)
                                                                                  aatcccTTTCGCTGCGGCTAAGCATAATATGCAAAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTc                                          >  2:6479421/6‑140 (MQ=1)
                                                                                    tctcTTTCGCTGAGGCTCCGCATCTTCTGCTTAACCTTGCACAGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCAc                                        <  2:6459127/137‑1 (MQ=0)
                                                                                     cggtttCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACGTGTCGGCACACGGTTTCAGGATCTCTTTCACt                                       >  2:3863842/4‑140 (MQ=0)
                                                                                      gcgttCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTc                                      <  1:6743271/137‑1 (MQ=1)
                                                                                      gcgttCGCTGCGGCTCCGCATCGTCAGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCCCCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTc                                      <  2:6725934/137‑1 (MQ=0)
                                                                                      gcattCGCTGCGGCTCAGGATCTTCAGCTTAACCTTGCACGGGATCGACACTCGCCCGGTAATTCTAAATCAGGCACGCCAGCACCCGGTATCGGGCGGTGAGTCCTTGGTGGCCGACGCTTCGAGGCTCTCTTTctggg                                      >  2:4330442/4‑136 (MQ=0)
                                                                                       ctgtCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTcc                                     >  2:4374538/4‑140 (MQ=1)
                                                                                                cgtcTCCGCATCTTCTGCTTAACCTTTCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGGAGGCACACGGGTTCAGGATCTCTTTCACTCCCCTTCCggg                            <  1:6767269/137‑1 (MQ=0)
                                                                                                 ggatCCGCATCTTCTGCTTAACCTGGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGAGCTCTTTCACTCCCCTACCgggg                           <  1:7592985/137‑1 (MQ=0)
                                                                                                        cagcttctGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCAGTCTACAAAAGGCAAGCCAACACCAGATAACGGGCTCTGACTACAACTAGGCACACGCTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCgttt                    >  1:524703/4‑140 (MQ=1)
                                                                                                         atgttctGCTTAACCTTGCACGGGATCGTAACTCGCAGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTc                   <  2:67744/137‑1 (MQ=1)
                                                                                                                   tacccTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCt         <  1:6490279/137‑1 (MQ=1)
                                                                                                                     acattGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCa       <  2:1393553/137‑1 (MQ=1)
                                                                                                                     acattGCACGGGATAGTAACTCGCCGGTTCATTCTACAAAAGTCACGCCATCTCCCGGTACCGGGCTCTGACTAGTTGTAGGCACACGTTTTCAGGATCTCTTTCAATCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCa       <  2:506012/137‑1 (MQ=1)
                                                                                                                      ccgtGCACGGGATCGTAACTCGCCGGTTCATTCTACACAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTGGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCAc      <  1:4764293/137‑1 (MQ=0)
                                                                                                                      ccgtGCACAGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCAc      <  2:6970452/137‑1 (MQ=1)
                                                                                                                          gcccGGGAGCGTAACTCGCCGGTTAATTCTACAAAAGGCACGCAATCACCCGCTCACGGGCTCTGACTACTTCGAGGCACACGGTTCCAGCCTCTCGTGCCCGCCCCTGCCGGGGTGCTTTTCACCTTTCCCGCACGGTa  <  2:2008523/137‑1 (MQ=1)
                                                                                                                              |                                                                                                                                       
AGTGTTACCTGAACTTCACCCTGGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTA  >  NZ_CP034943/2980034‑2980295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑