Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,991,245 G→T 22.9% intergenic (+46/‑161) gbsR → / → bslA transcriptional regulator GbsR/biofilm‑surface layer protein BslA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,991,2450GT22.9% 389.0 / 46.8 236intergenic (+46/‑161)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand):  ref base G (67/108);  new base T (58/3);  total (125/111)
Fisher's exact test for biased strand distribution p-value = 2.50e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

CTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAA  >  NZ_CP034943/2991109‑2991384
                                                                                                                                         |                                                                                                                                              
ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTaa                                                                                                                                             >  2:1985030/1‑140 (MQ=255)
ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTTTaa                                                                                                                                             >  1:5616726/1‑140 (MQ=255)
 tctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAAt                                                                                                                                            <  2:1487617/140‑1 (MQ=255)
   tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTCTTTTTTATGTAATta                                                                                                                                          >  2:4510702/1‑140 (MQ=255)
      aGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATtacta                                                                                                                                       <  2:5405024/140‑1 (MQ=255)
          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACACAGTAAAACAAAATCGCCTGGGAACCCGGGCGTTTTTTTGGTGTTTTTTATTTAATTACAACaaa                                                                                                                                  >  2:1372476/1‑140 (MQ=255)
          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTATTTTTTTTTTAATTAAAACaaa                                                                                                                                  >  2:6110633/1‑140 (MQ=255)
          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAATtactaacaa                                                                                                                                   >  2:4694147/1‑136 (MQ=255)
           aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAATCAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTATTTTTTTTTTAATtaataacaat                                                                                                                                  >  2:6656125/1‑135 (MQ=255)
           aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTAATTACTACAAAt                                                                                                                                 >  2:2133412/1‑140 (MQ=255)
           aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTAATAaaaaa                                                                                                                                 >  1:7395753/1‑139 (MQ=255)
           aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTAATACAAAt                                                                                                                                 >  2:2085008/1‑140 (MQ=255)
           aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATTTAATTAATAaaaaa                                                                                                                                 >  1:6946943/1‑139 (MQ=255)
           aCTATGATTGGCTCAAACGGCTTGCTTAATCGGTGAAGAGCGGGTAAATTTTTAAATTTATACCGATTGAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAAt                                                                                                                                 <  1:4694147/140‑1 (MQ=255)
                gATTGGCGTAACCGGCGTGCTGCATCGTGGCCGAGCGGGTAAATTTTTAAATTTACACCCATCGAAACAACCGACACAACAAACGCCTGGGAAACCGGGAGTTTTTTTGTACATTTTTATGGAATTACTACAAATTTGaa                                                                                                                           <  1:533258/140‑1 (MQ=255)
                 aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTAATAAAAATTTTaaa                                                                                                                          >  2:2021991/1‑140 (MQ=255)
                 aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTTaaa                                                                                                                          >  2:2418793/1‑140 (MQ=255)
                 aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTATTTTTTATGTAATTAATACAAATTTTaaa                                                                                                                          >  2:4292684/1‑140 (MQ=255)
                    ggCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTTAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAt                                                                                                                       <  2:3796039/140‑1 (MQ=255)
                    ggCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTAATACAAATTTTaaaaaa                                                                                                                       >  2:6783892/1‑139 (MQ=255)
                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAATCGCCTGGGAAACCGGGCGTTTTTTTTTTATTTTTTTTTTAATTAATACAAATTTTTAAAATa                                                                                                                      >  1:6608529/1‑140 (MQ=255)
                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGACACCGGGCGTTTTTGTTTTTTTTTTTTTGTTATTAATACAAATTTTaaaaaaa                                                                                                                      >  1:5018311/1‑138 (MQ=255)
                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTTTTAATAAAAAAAAATTTaaaaaaa                                                                                                                       >  1:6796825/1‑139 (MQ=255)
                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTTATTAATAAAAATTTTaaaaaaa                                                                                                                      >  1:3687933/1‑138 (MQ=255)
                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTATATTTTATTTAATTACTACAAAAATGaaaaaaa                                                                                                                      >  2:4269076/1‑138 (MQ=255)
                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGGTGTTTTTTATGTAATTAAGAAAAATTTTTAAAATa                                                                                                                      >  1:4280769/1‑140 (MQ=255)
                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTGTTATTTCTACAAAATTTTaaaaaa                                                                                                                       >  1:333486/1‑139 (MQ=255)
                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTTTTGTTTTTTATTTAATTACTAAAAAATTTAAAAATa                                                                                                                      >  2:3105469/1‑140 (MQ=255)
                       tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTTATTAGTAAAAATTTTAAAAATaac                                                                                                                    >  2:774444/1‑138 (MQ=255)
                            cgtcTTGCTTATTCTGGGCAGAGCTGGCAAATTTTTAAATTTATACCGATTCAAAAAAATTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGaaa                                                                                                               <  1:6313899/137‑1 (MQ=255)
                               cTTGCTGAATCGGTTCAGAGCGGGTAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAAc                                                                                                            <  2:2677616/140‑1 (MQ=255)
                               cTTGCTGAATCGGTGCAGAGCGGGGAACTTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTGTTTTTGTTTTTTATGTAATTACTACAAAGTTGACAAATAACGGaacacc                                                                                                            >  2:4186419/1‑136 (MQ=255)
                               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAATGTCAAACAAAAACGCCGGGGAAACCGGGCGTGTGTTTGTGCTTGTTTATTTAATTACGACAAATTTGTATAATACgggggagac                                                                                                            >  2:3575917/1‑134 (MQ=255)
                               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAATACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTCTTTTTTAGTTAATTTTAACAAAATTTGAAAATAAGGGaaaaaa                                                                                                            >  1:5380618/1‑139 (MQ=255)
                               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAATACAAAAACGCCTGGGAAACCGGGCGTGGTTTTGTTATTTTTTATGTAATTTCAACAAAATTTAAAAAAAAGGGGAAAAc                                                                                                            >  2:3842312/1‑140 (MQ=255)
                               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAAc                                                                                                            <  2:488257/140‑1 (MQ=255)
                               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTTTTGTTTTTTTTTTTTTTAATAAAAAATTTAAAAATAATGGaaaaaa                                                                                                            >  1:3062179/1‑139 (MQ=255)
                                ttGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAAATAAAAAAAATTTGAAAAATAAGGGAAAAACa                                                                                                           >  2:4352921/1‑140 (MQ=255)
                                ttGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTGTGTGTTCTTTTTTATGTAATTAATAACAATTTGAAAAATACGGGCAAAACa                                                                                                           >  2:4836986/1‑140 (MQ=255)
                                  gatGAATCTGTGAATATCTGGTAAATTTTTAAATTTATACCTTTTTTAAAAATTTTAAAAAAAAACTACTGTGAAACAGTTAGTTTTTTTGTTATTTTTTATTTTATTTATATTAATTTTAAAAATATGTGATAAACATc                                                                                                         <  2:5487004/138‑1 (MQ=255)
                                  gCTGAATCTTTGAAGAGCGGGGAAATTTTTAAATTTATACCTATTGAAAAAAAGTAAAAAAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATc                                                                                                         <  2:1227786/140‑1 (MQ=255)
                                   cTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGACACCGGGCGTTTTTTTTTTGTTTTTTATGTAATTACTACAAAATTGTATCAGACGGGTAAAAAAttc                                                                                                        >  2:7008190/1‑138 (MQ=255)
                                           gTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGCCGTGGTTTTTTTGTTTTTTATTTAAATTATACAAATTTTTAAAATGAGGGAAAAACATCGCAtatgat                                                                                                >  2:4943025/1‑135 (MQ=255)
                                                 agCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTAATTAAGACAAATTTTAAAAAAAAGGGAAAAAAAAACCCATTACTTACaaa                                                                                          >  1:5865964/1‑140 (MQ=255)
                                                     gttaaaTTTTTAAATTTATACCTATTTAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAatat                                                                                      <  2:5931772/137‑1 (MQ=255)
                                                     gcgAAATTTTTAAATTTATACCGATTGAAACAAATTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAatat                                                                                      <  2:4216463/138‑1 (MQ=255)
                                                      ggAAATTTTTAAATTTATAGCGATTGAAACAAAGTAAAACAAAAACTCCTGGGAAACCGGGCGTTTTTTTGTTATTTTTTATGTAATTAATACAAATTTGTATAATACGGGAAAAACATCCCCTTTAAGTCCcaaagagt                                                                                     >  2:5309717/1‑136 (MQ=255)
                                                      ggAAATTTTTAAATTTATACCGATTGACACACAGTAAAACAAAAACGCCTGGTAAACCGGGCGTGTGTGTGTTGTTATTTATTTAAATACTACAAATTTGAAAAATACAGCACAAACATACCATTTACTGCCAAAAAAtt                                                                                     >  2:565176/1‑140 (MQ=255)
                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAATCGCCTGGGAAACCGGGCGTTGTTTTGTTTTTTTTTTTGTTATTTATAAAAAATTTTAAAAAAAGGGTAAAAAATACAAATTAGTTGCAAAAAAtt                                                                                     >  1:3295991/1‑140 (MQ=255)
                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTAGTTTTTTTGTAATTAATACAAAATTGAAAAAAAAGGGAAAAACATACCAATTAAGTCCAAAataat                                                                                     >  2:3357092/1‑138 (MQ=255)
                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTTATTAATTAAAAATTTTAAAATAAAGGAAAAAAATCACAGTTAATTGAAAAAAAtt                                                                                     >  1:5852033/1‑140 (MQ=255)
                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTATTTAATTAAAAAAAAATTTAAAAAAAAGGGAAAAACAAACCAGTTAATTCAAAAAAAtt                                                                                     >  1:1413755/1‑140 (MQ=255)
                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTTTTATTAAAAAAAATTTGAAAAATAACGGAAAAACAAACCAGTTACTTGCAAAAAAtt                                                                                     >  1:3218671/1‑140 (MQ=255)
                                                       taaaTTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTCTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATAttt                                                                                    <  2:4039502/139‑1 (MQ=255)
                                                         aaTTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGa                                                                                  <  2:673827/140‑1 (MQ=255)
                                                           tttttAAATTTATACCGATTGAAACAAAGTAAACCAAAAACGCCTGGTAAACCGGTCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATg                                                                                <  2:1670103/140‑1 (MQ=255)
                                                           tttttAAATTTATACCGATTGAAACAAAGTAAACCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATg                                                                                <  2:5950550/140‑1 (MQ=255)
                                                           tttttAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTGTGTTTTTTATGTAATTAATAAAAATTTGAAAAAAAAGGGAAAAAAATACCATATAAGTACAAAATATatgatg                                                                                >  2:6245831/1‑140 (MQ=255)
                                                            ttttAAATTTATACCCATTCAAACAACGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                               <  1:2339183/140‑1 (MQ=255)
                                                                      atcccGTTTGAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                     <  1:1612185/137‑1 (MQ=255)
                                                                            aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTTTAATTAATACAAAATTTAAAAATACAGGCAAAAAATACCATTTTCTTACAAAAAATTTTGTGGTAAATTTATGTTGGtt                                                               >  1:7532574/1‑139 (MQ=255)
                                                                                aaaCAAATTAAAACAAAAACGCCTGCGAAACCGGGCGTTTTTTTGTTATTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                           <  2:7155612/140‑1 (MQ=255)
                                                                                aaaCAAAGAAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                           <  1:2857404/140‑1 (MQ=255)
                                                                                          aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  1:6098666/140‑1 (MQ=255)
                                                                                          aaacaaacACGCCTGGGAAACCGGGCGATTTTTTGTTCTTTTTTATGTAATTACTACAACTTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:2966818/140‑1 (MQ=255)
                                                                                          aaaaaaaaACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  1:862439/140‑1 (MQ=255)
                                                                                          aaaCAACCACGCCTGCGACACCCGGCGTTTTTTTGTTCTTTTTTATGTAATTACTCCAACTTTGCAAAATACGGGACAAACATCCCATTTACTTCCAAAATATTGGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:4547577/140‑1 (MQ=255)
                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  1:3001630/140‑1 (MQ=255)
                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  1:1232772/140‑1 (MQ=255)
                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  1:2418401/140‑1 (MQ=255)
                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  1:4173208/140‑1 (MQ=255)
                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:2297032/140‑1 (MQ=255)
                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  1:6458312/140‑1 (MQ=255)
                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  1:1655437/140‑1 (MQ=255)
                                                                                           taCAAAAACGCCTGGGAAACCGGGCGTGGTTTTTTTGTTTTTTATTTAATTACTAAAAATTTGAAAAATACGGGAAAAACATGCCATTTACTTAGAAAATATTTGGTTGTAATTTTTTGTAGGTTAAATTTGTAGTTTgg                                                >  2:7256067/2‑140 (MQ=255)
                                                                                           aacaaacaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGc                                               <  1:1061493/140‑1 (MQ=255)
                                                                                           aaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATGTAATTAATACAAATTTGAAAAATAAGGGAAAAACAACCCATTTACTTCAAAAATATTTGAAGGTAATTTTATGTAGGTAAAAATTGTATTTggg                                               >  2:1399777/1‑139 (MQ=255)
                                                                                           aaCAAAAAAGCCTGGGAAACCGGGCGTGTGTTTTGTCTTTTTTATTTTTTTAATAAAAAATTGTAAAAAAACGGACAAAACTAGCAGTTAGTTACAAAATATTTGATTTTAATTTTTTGTAGGTTAGATTTTTATTTgtc                                               >  2:1089973/1‑138 (MQ=255)
                                                                                             cAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCg                                             <  1:3796960/140‑1 (MQ=255)
                                                                                               aacaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                                           <  1:588313/137‑1 (MQ=255)
                                                                                                aaaCGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTAATACAAATTTGAAAAATAAGGGCAAAACATGCCATTTAAGTGCCAAATAGTTGATGTTTCCTTTATGTGGGTCAAATTTGTCTTTGGGCGtgc                                          >  2:6085839/1‑138 (MQ=255)
                                                                                                aaaCGCCTGGGAAACCGGGCGTTTTTGTGTGTTTTTTTATGTAATTACTACAAATTTGTACAAAAAGGGCAAAAAATCCCATGTAATTAGCAAATATATTATTGTAATTTGTTTTAGGTTAAATGTGTATTTGGGCGttg                                          >  2:2796125/1‑139 (MQ=255)
                                                                                                  aCGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTTATTAATACAAATTTGAAAAAAAAGGGGAAAAAATACCAATTACTTCCAAAAGATTTTATTGTTATTTTATTTAGGTAAAATTTGTATTTGGGCGTTGag                                        >  2:6487407/1‑140 (MQ=255)
                                                                                                    gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTATTTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                      <  1:7687582/140‑1 (MQ=255)
                                                                                                    gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGACAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                      <  1:5164815/140‑1 (MQ=255)
                                                                                                    gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                      <  1:6361356/140‑1 (MQ=255)
                                                                                                    gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                      <  1:4872118/140‑1 (MQ=255)
                                                                                                    gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                      <  1:2675818/140‑1 (MQ=255)
                                                                                                         ggAAACCGGGCTTTTTTTTGTTATTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGt                                 <  1:4349548/140‑1 (MQ=255)
                                                                                                         ggAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGt                                 <  1:1572754/140‑1 (MQ=255)
                                                                                                              ccGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa                            <  1:5530620/140‑1 (MQ=255)
                                                                                                                   cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                       <  2:6608529/140‑1 (MQ=255)
                                                                                                                   cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                       <  1:6866507/140‑1 (MQ=255)
                                                                                                                   agTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTACAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                       <  1:7064183/139‑1 (MQ=255)
                                                                                                                           ttGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAACACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCt                <  2:2512313/140‑1 (MQ=255)
                                                                                                                            tGTTATTGTTTATGTAATTACTACAAATTTGAAAAATACGGGATAAACATCCCATGTACTTCCAAAATATTAGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTg               <  2:7698904/140‑1 (MQ=255)
                                                                                                                               tattttttATTTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAat            <  2:5852033/138‑1 (MQ=255)
                                                                                                                                 ttttttATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAatat          >  2:5942368/1‑140 (MQ=255)
                                                                                                                                   ttttATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATAttt        >  1:2749957/1‑140 (MQ=255)
                                                                                                                                       aTGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTtat    <  1:1912869/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                              
CTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAA  >  NZ_CP034943/2991109‑2991384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: