Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,991,247 A→T 10.4% intergenic (+48/‑159) gbsR → / → bslA transcriptional regulator GbsR/biofilm‑surface layer protein BslA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,991,2470AT10.4% 653.0 / 11.0 234intergenic (+48/‑159)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand):  ref base A (95/111);  new base T (26/2);  total (121/113)
Fisher's exact test for biased strand distribution p-value = 1.14e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 238 total aligned reads displayed.

CTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACT  >  NZ_CP034943/2991109‑2991386
                                                                                                                                          |                                                                                                                                              
 tctAAAGCTTACTATGATTGGCTGAAACGGCTTGCGGAATCGGGGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAAt                                                                                                                                              <  2:4787946/140‑1 (MQ=255)
   tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTCTTTTTTATGTAATta                                                                                                                                            >  2:4510702/1‑140 (MQ=255)
      aGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATtacta                                                                                                                                         <  2:345075/140‑1 (MQ=255)
      aGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATtacta                                                                                                                                         <  2:5405024/140‑1 (MQ=255)
          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTATTTTTTTTTTAATTAAAACaaa                                                                                                                                    >  2:6110633/1‑140 (MQ=255)
          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTTTAAATAAAACaaa                                                                                                                                    >  1:6816452/1‑140 (MQ=255)
           aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTTTAATTAATAaaaaa                                                                                                                                   >  1:5652437/1‑139 (MQ=255)
           aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTAATACAAAt                                                                                                                                   >  2:2085008/1‑140 (MQ=255)
           aCTATGATTGGCTCAAACGGCTTGCTTAATCGGTGAAGAGCGGGTAAATTTTTAAATTTATACCGATTGAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAAt                                                                                                                                   <  1:4694147/140‑1 (MQ=255)
                tatgggCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTTAAACAACGTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGaa                                                                                                                              <  1:7428769/136‑1 (MQ=255)
                gATTGGCGTAACCGGCGTGCTGCATCGTGGCCGAGCGGGTAAATTTTTAAATTTACACCCATCGAAACAACCGACACAACAAACGCCTGGGAAACCGGGAGTTTTTTTGTACATTTTTATGGAATTACTACAAATTTGaa                                                                                                                              <  1:533258/140‑1 (MQ=255)
                 aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTTTTTAATTAATACAAAATTGaaa                                                                                                                             >  2:2418401/1‑140 (MQ=255)
                 aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTATTTTTTTTTTAAATAATAAAAAATTTaaa                                                                                                                             >  2:3177998/1‑140 (MQ=255)
                 aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTAATACAAATTTTaaa                                                                                                                             >  2:1232772/1‑140 (MQ=255)
                 aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATTTAATTAATAAAAATTTTaaa                                                                                                                             >  2:5886517/1‑140 (MQ=255)
                    ggCTTAAACGGCTTCCTGAATCGGTGCAGAGCGGTGAAATTTTTAAATTTATACCTATTTAAAAAAAGTAACACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTATTTACTACAAATTTGAAAAAt                                                                                                                          <  1:3375936/140‑1 (MQ=255)
                    ggCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTAATACAAATTTTaaaaaa                                                                                                                          >  2:6783892/1‑139 (MQ=255)
                    ggCTGAAACGGATTGCTGACTCGGTGACGCGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAt                                                                                                                          <  1:7756512/140‑1 (MQ=255)
                     gCTGAAACGGCTTGCTGAATCGGTGCATAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTCTGAAACCGGGCGTTTTTTTGTTATTTTTTATGTAAAAAATACAAATTTGaaaaaaa                                                                                                                         >  2:4501659/1‑138 (MQ=255)
                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTTATTAATAAAAATTTTaaaaaaa                                                                                                                         >  1:3687933/1‑138 (MQ=255)
                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTATATTTTATTTAATTACTACAAAAATGaaaaaaa                                                                                                                         >  2:4269076/1‑138 (MQ=255)
                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGGTGTTTTTTATGTAATTAAGAAAAATTTTTAAAATa                                                                                                                         >  1:4280769/1‑140 (MQ=255)
                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTGTTTTTGTTATTAATAAAAAATTTTAAAATa                                                                                                                         >  1:5038544/1‑140 (MQ=255)
                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTATTTTTTTTTTAATTAATACAAATTTTAAAAATa                                                                                                                         >  2:3815280/1‑140 (MQ=255)
                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTTTTGTTTTTTATTTAATTACTAAAAAATTTAAAAATa                                                                                                                         >  2:3105469/1‑140 (MQ=255)
                       tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTTATTAGTAAAAATTTTAAAAATaac                                                                                                                       >  2:774444/1‑138 (MQ=255)
                       tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTATTTTTTATGTAATTACTACAAAATTGAAAAATaag                                                                                                                       >  2:2095564/1‑138 (MQ=255)
                            cgtcTTGCTTATTCTGGGCAGAGCTGGCAAATTTTTAAATTTATACCGATTCAAAAAAATTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGaaa                                                                                                                 <  1:6313899/137‑1 (MQ=255)
                               cTTGCTGAATCGGTTCAGAGCGGGTAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAAc                                                                                                              <  2:2677616/140‑1 (MQ=255)
                               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAAc                                                                                                              <  2:488257/140‑1 (MQ=255)
                               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTTTTGTTTTTTTTTTTTTTAATAAAAAATTTAAAAATAATGGaaaaaa                                                                                                              >  1:3062179/1‑139 (MQ=255)
                                  gatGAATCTGTGAATATCTGGTAAATTTTTAAATTTATACCTTTTTTAAAAATTTTAAAAAAAAACTACTGTGAAACAGTTAGTTTTTTTGTTATTTTTTATTTTATTTATATTAATTTTAAAAATATGTGATAAACATc                                                                                                           <  2:5487004/138‑1 (MQ=255)
                                   cTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGACACCGGGCGTTTTTTTTTTGTTTTTTATGTAATTACTACAAAATTGTATCAGACGGGTAAAAAAttc                                                                                                          >  2:7008190/1‑138 (MQ=255)
                                           gTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGCCGTGGTTTTTTTGTTTTTTATTTAAATTATACAAATTTTTAAAATGAGGGAAAAACATCGCAtatgat                                                                                                  >  2:4943025/1‑135 (MQ=255)
                                               agagCGGGTAAATTTTTAAATTTATACCGATTGAAACAAATTAAAAAAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCa                                                                                              <  2:2743553/140‑1 (MQ=255)
                                                 agCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTAATTAAGACAAATTTTAAAAAAAAGGGAAAAAAAAACCCATTACTTACaaa                                                                                            >  1:5865964/1‑140 (MQ=255)
                                                     gggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTTTTATTAATAAAAATTTTAAAAAAACGGTGAAAAACACCCAATTTCTTGCAAAaaat                                                                                        >  2:1912869/1‑140 (MQ=255)
                                                      ggAAATTTTTAAATTTATAGCGATTGAAACAAAGTAAAACAAAAACTCCTGGGAAACCGGGCGTTTTTTTGTTATTTTTTATGTAATTAATACAAATTTGTATAATACGGGAAAAACATCCCCTTTAAGTCCcaaagagt                                                                                       >  2:5309717/1‑136 (MQ=255)
                                                      ggAAATTTTTAAATTTATACCGATTGACACACAGTAAAACAAAAACGCCTGGTAAACCGGGCGTGTGTGTGTTGTTATTTATTTAAATACTACAAATTTGAAAAATACAGCACAAACATACCATTTACTGCCAAAAAAtt                                                                                       >  2:565176/1‑140 (MQ=255)
                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAATCGCCTGGGAAACCGGGCGTTGTTTTGTTTTTTTTTTTGTTATTTATAAAAAATTTTAAAAAAAGGGTAAAAAATACAAATTAGTTGCAAAAAAtt                                                                                       >  1:3295991/1‑140 (MQ=255)
                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTTGGAAACCGGGCGTGTTTTTGTTGGTTTTTTTTTAATTACTAAACATTTTAAAAATTAGGGAAAAACATCCCATGTAATTCGAAAAAAtt                                                                                       >  2:6639398/1‑140 (MQ=255)
                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTGTGGTTGTTTTTTTTGTTATTACAACAAATTTTAAAAAAACGGGAAAAACATAACAGTTACATACAAAataag                                                                                       >  2:413198/1‑138 (MQ=255)
                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTTATTAATTAAAAATTTTAAAATAAAGGAAAAAAATCACAGTTAATTGAAAAAAAtt                                                                                       >  1:5852033/1‑140 (MQ=255)
                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTATTTAATTAAAAAAAAATTTAAAAAAAAGGGAAAAACAAACCAGTTAATTCAAAAAAAtt                                                                                       >  1:1413755/1‑140 (MQ=255)
                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTTTTATTAAAAAAAATTTGAAAAATAACGGAAAAACAAACCAGTTACTTGCAAAAAAtt                                                                                       >  1:3218671/1‑140 (MQ=255)
                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTGGGTTTTTTTGTAATTACTACGAATTTGTAAAATACGGGTGAAACATGCCAGTTTCTTGCCAAATAtt                                                                                       >  2:1393552/1‑140 (MQ=255)
                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTGTTTGTTGTTTTTTTTTTTATTAATAAAAATTTTAAAAATAAGGGAAAAAAAACACACTTAATTCAaaaaaaat                                                                                       >  1:76108/1‑136 (MQ=255)
                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGGTTTTTGGTTTTTTTTGTAATTAAAACAAAATTTGAAAATAAAGGGAAAAAATCCCCTTTTCTTGCaaaagagt                                                                                       >  1:7060301/1‑136 (MQ=255)
                                                      ggAAATTTTTAAAGTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTGTGTTTTTTGTTATGTATATATTAAAAATTTGAAAAAAACCGCACAAACAAACCAGTTAAGTACAAAATAtt                                                                                       >  1:5386383/1‑140 (MQ=255)
                                                       taaaTTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTCTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATAttt                                                                                      <  2:4039502/139‑1 (MQ=255)
                                                         aaTTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGa                                                                                    <  2:673827/140‑1 (MQ=255)
                                                           tttttAAATTTATACCGATTGAAACAAAGTAAACCAAAAACGCCTGGTAAACCGGTCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATg                                                                                  <  2:1670103/140‑1 (MQ=255)
                                                           tttttAAATTTATACCGATTGAAACAAAGTAAACCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATg                                                                                  <  2:5950550/140‑1 (MQ=255)
                                                           tttttAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTGTGTTTTTTATGTAATTAATAAAAATTTGAAAAAAAAGGGAAAAAAATACCATATAAGTACAAAATATatgatg                                                                                  >  2:6245831/1‑140 (MQ=255)
                                                            ttttAAATTTATACCCATTCAAACAACGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                                 <  1:2339183/140‑1 (MQ=255)
                                                                      atcccGTTTGAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                       <  1:1612185/137‑1 (MQ=255)
                                                                            aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTATGTAATTAATACAAAATTGTAAAATACGGGCAAAACCAGCCATTTTATTACAAAATATTTTTTGGTAATTTTgtgtttgtt                                                                 >  2:1041490/1‑135 (MQ=255)
                                                                                aaaaaaACTAAAACAAAAACGCCTGGGAAACCGGGCGATTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                             <  1:6763571/140‑1 (MQ=255)
                                                                                aaaCAAATTAAAACAAAAACGCCTGCGAAACCGGGCGTTTTTTTGTTATTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                             <  2:7155612/140‑1 (MQ=255)
                                                                                aaaCAAAGAAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                             <  1:2857404/140‑1 (MQ=255)
                                                                                      aGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTGTAATTACTACAAATTTGAAAAAAAAGGGCAAAACAACCCCATTACATGCAAAATATTTTTTTGTAATTTTATGTTGGTTAAAATTGttt                                                       >  2:2506238/1‑138 (MQ=255)
                                                                                          aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  1:6098666/140‑1 (MQ=255)
                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  1:4173208/140‑1 (MQ=255)
                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  1:3001630/140‑1 (MQ=255)
                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  1:2418793/140‑1 (MQ=255)
                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  1:6458312/140‑1 (MQ=255)
                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  2:2877029/140‑1 (MQ=255)
                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  1:1655437/140‑1 (MQ=255)
                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  1:1232772/140‑1 (MQ=255)
                                                                                          aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACGACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  1:5886517/140‑1 (MQ=255)
                                                                                           taCAAAAACGCCTGGGAAACCGGGCGTGGTTTTTTTGTTTTTTATTTAATTACTAAAAATTTGAAAAATACGGGAAAAACATGCCATTTACTTAGAAAATATTTGGTTGTAATTTTTTGTAGGTTAAATTTGTAGTTTgg                                                  >  2:7256067/2‑140 (MQ=255)
                                                                                           aacaaacaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGc                                                 <  1:1061493/140‑1 (MQ=255)
                                                                                           aaCAAAAACGCCTGGGAAACCGGGCGTTTGTTTGTTGTTTTTTATTTAAATACAAAAAAATTTAAAAATAAGGGCAAAAACTCCCAGTTACTTAAAAAAAATTTGTTTGTTATTTTATTTAGGTTAAATTTGTtttttgg                                                 >  2:4303607/1‑137 (MQ=255)
                                                                                           aaCAAAAACGCCTGGGAAACCGGGCGTTTGTTTGGTGTTTTTTATTTAATTAATACAAAATTTTAAAATACGGGAAAAAAATACCAGTTAAGTGCAAAAAATTTGTTTGTAATTTTGTGTAGGTAAAAGTTGTATTTGGc                                                 >  2:3791064/1‑140 (MQ=255)
                                                                                           aaCAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTGTAATTACAAAAAATTTTTAAAAAAAGGGAAAAAAAACCCATTTAATTCGAAAATATTTGTTTGTAATTTTATTTGGGTAAAAATTGTTTTTTGc                                                 >  2:428514/1‑140 (MQ=255)
                                                                                             cAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCg                                               <  1:3796960/140‑1 (MQ=255)
                                                                                               aaaaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                                             <  1:7299403/140‑1 (MQ=255)
                                                                                                aaaCGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTAATACAAATTTGAAAAATAAGGGCAAAACATGCCATTTAAGTGCCAAATAGTTGATGTTTCCTTTATGTGGGTCAAATTTGTCTTTGGGCGtgc                                            >  2:6085839/1‑138 (MQ=255)
                                                                                                aaaCGCCTGGGAAACCGGGCGTGTTTTTTGTGTTTTTTATTTAATTACTACAAAATTTAAAAATAACGGAAAAACAACCCAATTTCTTCCAAAACATTTGGTGGTAATTTTTTTTAGGGAAAATTTGTATTTGGTCGtta                                            >  2:198775/1‑139 (MQ=255)
                                                                                                  aCGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTTATTAATACAAATTTGAAAAAAAAGGGGAAAAAATACCAATTACTTCCAAAAGATTTTATTGTTATTTTATTTAGGTAAAATTTGTATTTGGGCGTTGag                                          >  2:6487407/1‑140 (MQ=255)
                                                                                                    gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGACAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                        <  1:5164815/140‑1 (MQ=255)
                                                                                                    gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                        <  1:6361377/140‑1 (MQ=255)
                                                                                                    gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                        <  1:4872118/140‑1 (MQ=255)
                                                                                                    gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                        <  1:2675818/140‑1 (MQ=255)
                                                                                                    gCCTGGGAAACCGGGAGTTTTTTTGTTCTATTTTATGTAATGACGACAAATGTGAAAAATACGGGAAAAACATCCCATCTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                        <  2:4659374/140‑1 (MQ=255)
                                                                                                         ggAAACCGGGCTTTTTTTTGTTATTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGt                                   <  1:4349548/140‑1 (MQ=255)
                                                                                                         ggAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGt                                   <  1:1572754/140‑1 (MQ=255)
                                                                                                              ccGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa                              <  1:5530620/140‑1 (MQ=255)
                                                                                                                   cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                         <  2:6608529/140‑1 (MQ=255)
                                                                                                                   cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                         <  1:6937126/140‑1 (MQ=255)
                                                                                                                        ttttGTTATTTTTTATGTAATGACTACAAATTTGAATAATACGGGAAAAACATCCCATTTACTTCCAAACTATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc                    <  2:4201936/140‑1 (MQ=255)
                                                                                                                          ttGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCt                  <  2:1134402/140‑1 (MQ=255)
                                                                                                                           tGTTATTGTTTATGTAATTACTACAAATTTGAAAAATACGGGATAAACATCCCATGTACTTCCAAAATATTAGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTg                 <  2:7698904/140‑1 (MQ=255)
                                                                                                                              tattttttATTTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAat              <  2:5852033/138‑1 (MQ=255)
                                                                                                                              tCTTTTTTATGTAATTAATACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAat              <  2:1415862/140‑1 (MQ=255)
                                                                                                                                ttttttATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAatat            >  2:5942368/1‑140 (MQ=255)
                                                                                                                                  ttttATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGTATAttt          >  2:2291933/1‑140 (MQ=255)
                                                                                                                                  ttttATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATAttt          >  1:2749957/1‑140 (MQ=255)
                                                                                                                                      aTGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTtat      <  1:1912869/140‑1 (MQ=255)
                                                                                                                                          aaTTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATAGTTGTTGGTAATTTTATGTAGGTAAAATTTGTATTTGGGCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACt  >  2:4759253/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                              
CTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACT  >  NZ_CP034943/2991109‑2991386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: