Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 183,034 A→G 26.7% M104T (ATG→ACG)  feuA ← iron ABC transporter substrate‑binding protein FeuA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943183,0340AG26.7% 729.0 / 312.1 421M104T (ATG→ACG) feuAiron ABC transporter substrate‑binding protein FeuA
Reads supporting (aligned to +/- strand):  ref base A (126/182);  new base G (39/73);  total (165/256)
Fisher's exact test for biased strand distribution p-value = 3.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 424 total aligned reads displayed.

TTCCAGTTTGAAGAGATATGGGAAACGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAGCAGTTTCGCGTCTTCCATCGATTCTACGCTTCCCGTAATGGC  >  NZ_CP034943/182896‑183173
                                                                                                                                          |                                                                                                                                           
ttccAGTTTGAAGAGATATGGGAAACGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCAt                                                                                                                                            >  2:3344696/1‑140 (MQ=255)
  ccAGTTTGAAGAGATATGGGAAACGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGttt                                                                                                                                          >  1:5596544/1‑140 (MQ=255)
               atatGGGAAACGGGGCTTGTAGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGACCTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGc                                                                                                                             >  2:1575865/1‑140 (MQ=255)
                tatGGGAAACGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCt                                                                                                                            <  2:4810785/140‑1 (MQ=255)
                tatGGGAAACGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCt                                                                                                                            <  1:7225938/140‑1 (MQ=255)
                tatGGGAAACGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCt                                                                                                                            <  2:5472140/140‑1 (MQ=255)
                 atGGGAAACGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCtt                                                                                                                           >  2:4312535/1‑140 (MQ=255)
                 atGGGAAACGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCAGGCTTCATTTCAAGAATTTTTTCAATCTTTGGCTCCATTTTTTCTACGGTTGCtt                                                                                                                           >  2:6116364/1‑140 (MQ=255)
                  tGGGAAACGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTc                                                                                                                          <  1:3308486/140‑1 (MQ=255)
                       aaCGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCtt                                                                                                                     <  1:1478405/140‑1 (MQ=255)
                       aaCGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCtt                                                                                                                     <  2:5541796/140‑1 (MQ=255)
                       aaCGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCtt                                                                                                                     <  2:7028404/140‑1 (MQ=255)
                       aaCGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCtt                                                                                                                     <  2:2832822/140‑1 (MQ=255)
                       aaCGGGGAGTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCtt                                                                                                                     <  2:5864318/140‑1 (MQ=255)
                        aCGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCttt                                                                                                                    >  2:5808854/1‑140 (MQ=255)
                        aCGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCttt                                                                                                                    >  2:506539/1‑140 (MQ=255)
                         cGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTa                                                                                                                   >  1:1028718/1‑140 (MQ=255)
                         cGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTa                                                                                                                   >  1:3568790/1‑140 (MQ=255)
                         cGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTa                                                                                                                   >  2:2362093/1‑140 (MQ=255)
                         cGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTa                                                                                                                   >  2:195856/1‑140 (MQ=255)
                         cGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTa                                                                                                                   >  1:5054152/1‑140 (MQ=255)
                         cGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGATTTGCTCCATTTTTTCTACGGATGCTTAGGCTTTa                                                                                                                   >  2:7452150/1‑140 (MQ=255)
                         cGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTGGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCTATGTTTGGCTCGATTTTTTCTCCGGTTGGGTCgggtgta                                                                                                                   >  1:124929/1‑135 (MQ=255)
                          ggggATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTAt                                                                                                                  >  2:6802144/1‑140 (MQ=255)
                             gATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAg                                                                                                               <  1:742859/140‑1 (MQ=255)
                                tGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTTCTTCGGCTTTATCAGTGa                                                                                                            <  1:6168128/140‑1 (MQ=255)
                                tGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGa                                                                                                            <  2:5742699/140‑1 (MQ=255)
                                tGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGa                                                                                                            <  1:838931/140‑1 (MQ=255)
                                tGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGa                                                                                                            <  2:6463649/140‑1 (MQ=255)
                                tGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGa                                                                                                            <  2:1296171/140‑1 (MQ=255)
                                tGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGa                                                                                                            <  1:4926067/140‑1 (MQ=255)
                                tGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGa                                                                                                            <  1:198362/140‑1 (MQ=255)
                                tGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGa                                                                                                            <  2:3502315/140‑1 (MQ=255)
                                tGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGa                                                                                                            <  1:3109557/140‑1 (MQ=255)
                                tGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGa                                                                                                            <  1:2338963/140‑1 (MQ=255)
                                tGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGa                                                                                                            <  2:775513/140‑1 (MQ=255)
                                tGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGa                                                                                                            <  1:5274502/140‑1 (MQ=255)
                                tGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGa                                                                                                            <  2:5203393/140‑1 (MQ=255)
                                tGTCGGGCCGGCAGTGCTGATTTTTTGCAGCGTGTTTGCCGGGAACTTTGTGCAAGCAAGGATGACATCGGGCTTCATTTCAAGAAATTTTTCAATGTTTGGCTCCATTTGTTCTCCGGTTGCTTCGGCTTTATCAGTGa                                                                                                            <  2:7703368/140‑1 (MQ=255)
                                tGTCGAGCCGGCAGTACTGATTTTTTAAAAAGAATAAACCGCGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTAAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCTGATTTATCAGtaa                                                                                                            >  2:3650301/1‑138 (MQ=255)
                                  tCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATg                                                                                                          <  2:104427/140‑1 (MQ=255)
                                     tGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCt                                                                                                       <  2:1334968/140‑1 (MQ=255)
                                     tGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCt                                                                                                       <  2:865618/140‑1 (MQ=255)
                                     tGCCGCCAGTGCTGATTTTGTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGTTGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTGGTCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCt                                                                                                       <  1:5312725/140‑1 (MQ=255)
                                      gCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCtt                                                                                                      >  2:5968614/1‑140 (MQ=255)
                                       ccGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCACGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTAt                                                                                                                  <  1:7616356/127‑1 (MQ=255)
                                       ccGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGc                                                                                                                       >  2:2656933/1‑122 (MQ=255)
                                       ccGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTAt                                                                                                                  >  2:7616356/1‑127 (MQ=255)
                                          gCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGaa                                                                                                  <  1:2808013/140‑1 (MQ=255)
                                          gCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGaa                                                                                                  <  1:6069221/140‑1 (MQ=255)
                                          gCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGaa                                                                                                  <  2:6956861/140‑1 (MQ=255)
                                          gCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGaa                                                                                                  <  2:7428096/140‑1 (MQ=255)
                                          gCAGTGCTGATTGTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGaa                                                                                                  <  1:6640907/140‑1 (MQ=255)
                                            aGTGCTGATTTTTTGCAGCGTTTTTTCCGTGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACa                                                                                                <  2:2243137/140‑1 (MQ=255)
                                            aGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACa                                                                                                <  2:1415473/140‑1 (MQ=255)
                                            aGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGGTGCTTCGGCTTTATCAGTGATGTCTTTGAACa                                                                                                <  2:2516086/140‑1 (MQ=255)
                                            aGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACa                                                                                                >  2:4672306/1‑140 (MQ=255)
                                            aGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGATCCATTTTTTCTGCGGTTGCTTAGGCTTTATAAGTGATGTCTTTGAACa                                                                                                >  2:4234139/1‑140 (MQ=255)
                                                 tGATTTTTTGCAGCGTTTTTTCCGGTAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCa                                                                                           <  2:1912394/140‑1 (MQ=255)
                                                   aTTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAgg                                                                                         <  1:4080153/140‑1 (MQ=255)
                                                    ttttttGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAggg                                                                                        >  1:1576681/1‑140 (MQ=255)
                                                             gCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCg                                                                               <  1:1739594/140‑1 (MQ=255)
                                                             gCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCg                                                                               >  2:2876046/1‑140 (MQ=255)
                                                             gCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCg                                                                               <  1:4405432/140‑1 (MQ=255)
                                                             gCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCg                                                                               <  2:725760/140‑1 (MQ=255)
                                                               gTTTGTTCCGGGAAGTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGATCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGga                                                                             <  2:4184045/140‑1 (MQ=255)
                                                                                tgttgAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATTAAATAGCGCCtt                                                            >  2:4464815/1‑140 (MQ=255)
                                                                                 gttgAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTg                                                           <  2:5618512/140‑1 (MQ=255)
                                                                                 gttgAAGCAAGGAAGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTg                                                           <  1:6325494/140‑1 (MQ=255)
                                                                                    gAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCg                                                                               <  2:3130346/117‑1 (MQ=255)
                                                                                      aGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGAt                                                      <  1:6594877/140‑1 (MQ=255)
                                                                                      aGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGAt                                                      <  2:4933114/140‑1 (MQ=255)
                                                                                      aGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGAt                                                      <  1:3828166/140‑1 (MQ=255)
                                                                                       gCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATg                                                     >  1:2054328/1‑140 (MQ=255)
                                                                                          aGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAAc                                                  >  2:3445297/1‑140 (MQ=255)
                                                                                           ggATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACa                                                 <  1:5497076/140‑1 (MQ=255)
                                                                                                aCATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAg                                            <  1:5661025/140‑1 (MQ=255)
                                                                                                aCATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAg                                            <  2:3589478/140‑1 (MQ=255)
                                                                                                 cATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACAACAAGc                                           >  2:1822841/1‑140 (MQ=255)
                                                                                                      ggCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAGCAGttt                                      >  1:6449447/1‑140 (MQ=255)
                                                                                                      ggCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAGCAGttt                                      >  1:3747389/1‑140 (MQ=255)
                                                                                                       gCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAGCAGTTTc                                     >  1:256609/1‑140 (MQ=255)
                                                                                                        cTTCATTTCAATAATTTTTTCTATGTATGGCTTGATTTTTTCTCCTGTTTCTTAGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCTGAGAATGTAATCGCGCGTTGCGGATGAACATCACGCAGTTTcg                                    <  2:7176000/140‑1 (MQ=255)
                                                                                                        cTTCAGTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAGCAGTTTcg                                    <  2:5367247/140‑1 (MQ=255)
                                                                                                           cATTTCAAGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAGCAGTTTCGCGt                                 >  1:6865484/1‑140 (MQ=255)
                                                                                                           cATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAAAATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAATCAGTTTCGCGt                                 >  2:663080/1‑140 (MQ=255)
                                                                                                            aTTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGTAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAGCAGTTTCGCGTc                                >  1:6750382/1‑140 (MQ=255)
                                                                                                            aTTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGAt                                                      >  2:6383222/1‑118 (MQ=255)
                                                                                                            aTTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAGCAGTTTCGCGTc                                >  1:7687341/1‑140 (MQ=255)
                                                                                                            aTTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAGCAGTTTCGCGTc                                >  1:6988484/1‑140 (MQ=255)
                                                                                                             tttAAAGAATTTTTTCAAGGTGTGGCTCCATTTTTTCTCCGGGTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATGTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAGCAGTTTCGCGTCt                               <  2:3927258/140‑1 (MQ=255)
                                                                                                              ttCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAGCAGTTTCGCGTCtt                              >  1:981414/1‑140 (MQ=255)
                                                                                                              ttCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAGCAGTTTCGCGTCtt                              >  1:894925/1‑140 (MQ=255)
                                                                                                                  aGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAGCAGTTTCGCGTCTTCCAt                          <  1:7515063/140‑1 (MQ=255)
                                                                                                                  aGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAGCAGTTTCGCGTCTTCCAt                          <  1:2922492/140‑1 (MQ=255)
                                                                                                                  aGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAGCAGTTTCGCGTCTTCCAt                          <  1:5808854/140‑1 (MQ=255)
                                                                                                                  aGAATTTTTTCAATGTTTGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAGCAGTTTCGCGTCTTCCAt                          <  1:2248702/140‑1 (MQ=255)
                                                                                                                  aGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAGCAGTTTCGCGTCTTCCAt                          >  2:7330924/1‑140 (MQ=255)
                                                                                                                  aGAATTTGGTCCATGTTGGGCTCCGTTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAGCAGTTTCGCGTCTTCCAt                          <  1:4436400/140‑1 (MQ=255)
                                                                                                                        ttttCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCaa                                             <  2:1146988/115‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
TTCCAGTTTGAAGAGATATGGGAAACGGGGATTGTCGTGCCGGCAGTGCTGATTTTTTGCAGCGTTTTTTCCGGGAACTTTGTTGAAGCAAGGATGACATCTGGCTTCATTTCAAGAATTTTTTCAATGTTTGGCTCCATTTTTTCTCCGGTTGCTTCGGCTTTATCAGTGATGTCTTTGAACATATCAGGGAATTTGCCGGAGAATGAAATCGCGCCTTGCGGATGAACATCAAGCAGTTTCGCGTCTTCCATCGATTCTACGCTTCCCGTAATGGC  >  NZ_CP034943/182896‑183173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: