Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,991,252 C→A 29.5% intergenic (+53/‑154) gbsR → / → bslA transcriptional regulator GbsR/biofilm‑surface layer protein BslA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,991,2520CA29.5% 326.9 / 58.6 234intergenic (+53/‑154)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand):  ref base C (46/107);  new base A (72/2);  total (125/109)
Fisher's exact test for biased strand distribution p-value = 1.33e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

AGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTT  >  NZ_CP034943/2991115‑2991390
                                                                                                                                           |                                                                                                                                            
aGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATtacta                                                                                                                                            <  2:345075/140‑1 (MQ=255)
aGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATtacta                                                                                                                                            <  2:5405024/140‑1 (MQ=255)
    tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACACAGTAAAACAAAATCGCCTGGGAACCCGGGCGTTTTTTTGGTGTTTTTTATTTAATTACAACaaa                                                                                                                                       >  2:1372476/1‑140 (MQ=255)
    tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTATTTTTTTTTTAATTAAAACaaa                                                                                                                                       >  2:6110633/1‑140 (MQ=255)
    tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTTTAATTAGTAaaaa                                                                                                                                       >  1:1507850/1‑140 (MQ=255)
    tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAATtactaacaa                                                                                                                                        >  2:4694147/1‑136 (MQ=255)
    tACTAGGAGTGGCTGAAACGGCTTGCTGACTCGGTGCAGAGCGGGGAAATTTTTAAATGTATACCGATTGAAACACAGTACAACAAAAACGCCTGGGAAACCGGGCGTTTTTTGTGTGTTTTTTTTGTAGTTACAACaaa                                                                                                                                       >  1:1611212/1‑140 (MQ=255)
     aCTATGATTGGCTCAAACGGCTTGCTTAATCGGTGAAGAGCGGGTAAATTTTTAAATTTATACCGATTGAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAAt                                                                                                                                      <  1:4694147/140‑1 (MQ=255)
      cTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTTTGTTATTAATAaaaaat                                                                                                                                     >  1:2743553/1‑138 (MQ=255)
          tatgggCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTTAAACAACGTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGaa                                                                                                                                 <  1:7428769/136‑1 (MQ=255)
           aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTTTGTTATTAATACAAAATTTaaa                                                                                                                                >  2:2675818/1‑140 (MQ=255)
           aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTATTTTTTTTTTAAATAATAAAAAATTTaaa                                                                                                                                >  2:3177998/1‑140 (MQ=255)
           aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATTTAATTAATAAAAATTTTaaa                                                                                                                                >  2:5886517/1‑140 (MQ=255)
           aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTTTGTAATTAATAAAAAattttaa                                                                                                                                >  2:4872118/1‑136 (MQ=255)
            ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTAATACAAATTTTaaaa                                                                                                                               >  2:5607991/1‑140 (MQ=255)
              ggCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTAATACAAATTTTaaaaaa                                                                                                                             >  2:6783892/1‑139 (MQ=255)
              ggCTGAAACGGATTGCTGACTCGGTGACGCGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAt                                                                                                                             <  1:7756512/140‑1 (MQ=255)
               gCTGAAACGGCTTGCTGAATCGGTGCATAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTCTGAAACCGGGCGTTTTTTTGTTATTTTTTATGTAAAAAATACAAATTTGaaaaaaa                                                                                                                            >  2:4501659/1‑138 (MQ=255)
               gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTTTTAATAAAAAAAAATTTaaaaaaa                                                                                                                             >  1:6796825/1‑139 (MQ=255)
               gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATTTTAATTCTACAAAATTGaaaaaaa                                                                                                                            >  1:5856696/1‑138 (MQ=255)
               gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTATATTTTATTTAATTACTACAAAAATGaaaaaaa                                                                                                                            >  2:4269076/1‑138 (MQ=255)
               gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGGTGTTTTTTATGTAATTAAGAAAAATTTTTAAAATa                                                                                                                            >  1:4280769/1‑140 (MQ=255)
               gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTGTTTTTGTTATTAATAAAAAATTTTAAAATa                                                                                                                            >  1:5038544/1‑140 (MQ=255)
               gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTGTTATTTCTACAAAATTTTaaaaaa                                                                                                                             >  1:333486/1‑139 (MQ=255)
               gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTGTTATTTATTCAAATTTTtaaaaaa                                                                                                                            >  1:6395426/1‑138 (MQ=255)
               gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTATTTTTTTTTTAATTAATACAAATTTTAAAAATa                                                                                                                            >  2:3815280/1‑140 (MQ=255)
                 tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTTATTAGTAAAAATTTTAAAAATaac                                                                                                                          >  2:774444/1‑138 (MQ=255)
                 tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTATTTTTTATGTAATTACTACAAAATTGAAAAATaag                                                                                                                          >  2:2095564/1‑138 (MQ=255)
                   aaaCGGCTTGCTGAATCGTTGCAGAGCGGGTAATTTTTTAAATTTATACCTATTCAAAAAAAGTAAAAAAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACggg                                                                                                                        <  1:554218/140‑1 (MQ=255)
                      cgtcTTGCTTATTCTGGGCAGAGCTGGCAAATTTTTAAATTTATACCGATTCAAAAAAATTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGaaa                                                                                                                     <  1:6313899/137‑1 (MQ=255)
                         cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTGTGTTTTTTTTTTAATTTATAACAATTTGAAAAATAAGGGaaaaaa                                                                                                                  >  1:3529419/1‑139 (MQ=255)
                         cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTTTTGTTTTTTTTTTTTTTAATAAAAAATTTAAAAATAATGGaaaaaa                                                                                                                  >  1:3062179/1‑139 (MQ=255)
                          ttGCTTAATCGGTGCAGCGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAACACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACa                                                                                                                 <  1:6951133/140‑1 (MQ=255)
                          ttGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATTTAATTAATACAAATTTTAAAAATAAGGGaaaaaaa                                                                                                                 >  2:3991153/1‑138 (MQ=255)
                            gatGAATCTGTGAATATCTGGTAAATTTTTAAATTTATACCTTTTTTAAAAATTTTAAAAAAAAACTACTGTGAAACAGTTAGTTTTTTTGTTATTTTTTATTTTATTTATATTAATTTTAAAAATATGTGATAAACATc                                                                                                               <  2:5487004/138‑1 (MQ=255)
                            gCTGAATCTTTGAAGAGCGGGGAAATTTTTAAATTTATACCTATTGAAAAAAAGTAAAAAAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATc                                                                                                               <  2:1227786/140‑1 (MQ=255)
                             cTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGACACCGGGCGTTTTTTTTTTGTTTTTTATGTAATTACTACAAAATTGTATCAGACGGGTAAAAAAttc                                                                                                              >  2:7008190/1‑138 (MQ=255)
                                 actggggCAGAGCGGGGAAATTTTTAAATTTATACCGATTTAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCAtt                                                                                                          <  1:5944118/134‑1 (MQ=255)
                                   cGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTTTAATTAATAAAAATTTGAAAAATACTGGAAAAACATGCCAATTa                                                                                                        >  2:476894/1‑140 (MQ=255)
                                     gTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGCCGTGGTTTTTTTGTTTTTTATTTAAATTATACAAATTTTTAAAATGAGGGAAAAACATCGCAtatgat                                                                                                      >  2:4943025/1‑135 (MQ=255)
                                         agagCGGGTAAATTTTTAAATTTATACCGATTGAAACAAATTAAAAAAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCa                                                                                                  <  2:2743553/140‑1 (MQ=255)
                                               gggACATTTATAAAGTGATACCGATTCACACAAAGTAAAACAAACACGCCGCGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAatat                                                                                            <  2:4528374/140‑1 (MQ=255)
                                               gggAAATTTTTAAATTTATACCTATTGAAACAAAGTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAatat                                                                                            <  2:6034270/140‑1 (MQ=255)
                                               gggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTATTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAatat                                                                                            <  2:1564782/140‑1 (MQ=255)
                                                ggAAATTTTTAAATTTATCCCGAGTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTACTTACTACCAAATTGAAAAATTCCGGTAAAACATGCCAGTTACATACAAAAAAtt                                                                                           >  2:5204667/1‑140 (MQ=255)
                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAATCGCCTGGGAAACCGGGCGTTGTTTTGTTTTTTTTTTTGTTATTTATAAAAAATTTTAAAAAAAGGGTAAAAAATACAAATTAGTTGCAAAAAAtt                                                                                           >  1:3295991/1‑140 (MQ=255)
                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTTGGAAACCGGGCGTGTTTTTGTTGGTTTTTTTTTAATTACTAAACATTTTAAAAATTAGGGAAAAACATCCCATGTAATTCGAAAAAAtt                                                                                           >  2:6639398/1‑140 (MQ=255)
                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTGTGGTTGTTTTTTTTGTTATTACAACAAATTTTAAAAAAACGGGAAAAACATAACAGTTACATACAAAataag                                                                                           >  2:413198/1‑138 (MQ=255)
                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTTTTATTTATACAAAATTTTTAAATAATGGAAAAAAAAACCCATTTATTACAAAatagt                                                                                           >  1:2337531/1‑138 (MQ=255)
                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTATTTAATTAAAAAAAAATTTAAAAAAAAGGGAAAAACAAACCAGTTAATTCAAAAAAAtt                                                                                           >  1:1413755/1‑140 (MQ=255)
                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTTTTATTAAAAAAAATTTGAAAAATAACGGAAAAACAAACCAGTTACTTGCAAAAAAtt                                                                                           >  1:3218671/1‑140 (MQ=255)
                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTGTAAGTAATACAAATTTGTAAAATAAGGGGCAAACATCCCCTGTACTTGGAAAAGAtt                                                                                           >  1:6654868/1‑140 (MQ=255)
                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTGTTGGTGTTGTTTTTTTTATTTATTAAAATTTTTAAAATACGGGGAAAAAGTCCCCAGTTGTTCCAAAattgt                                                                                           >  1:5379678/1‑137 (MQ=255)
                                                ggAAATTTTTAAAGTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTGTGTTTTTTGTTATGTATATATTAAAAATTTGAAAAAAACCGCACAAACAAACCAGTTAAGTACAAAATAtt                                                                                           >  1:5386383/1‑140 (MQ=255)
                                                   aaTTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGa                                                                                        <  2:673827/140‑1 (MQ=255)
                                                     tttttAAATTTATACCGATTGAAACAAAGTAAACCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATg                                                                                      <  2:5950550/140‑1 (MQ=255)
                                                     tttttAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTGTGTTTTTTATGTAATTAATAAAAATTTGAAAAAAAAGGGAAAAAAATACCATATAAGTACAAAATATatgatg                                                                                      >  2:6245831/1‑140 (MQ=255)
                                                                     taTTTAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                      <  1:3017986/139‑1 (MQ=255)
                                                                      aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTGTTTATTTAAATACAACAAATTTGAAAAATACGGGGAAAACCTCACAGTTAAATCCAAAATATTTTATTGTAAGTTTATGTAGGTa                                                                     >  2:232450/1‑140 (MQ=255)
                                                                      aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTATGTAATTAATACAAAATTGTAAAATACGGGCAAAACCAGCCATTTTATTACAAAATATTTTTTGGTAATTTTgtgtttgtt                                                                     >  2:1041490/1‑135 (MQ=255)
                                                                          aaaCAAATTAAAACAAAAACGCCTGCGAAACCGGGCGTTTTTTTGTTATTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                 <  2:7155612/140‑1 (MQ=255)
                                                                          aaaCAAAGTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTACTTACTACAAATTTGAAAAATACGGGAACAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                 <  2:1732104/140‑1 (MQ=255)
                                                                          aaaCAAAGAAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                 <  1:2857404/140‑1 (MQ=255)
                                                                                aGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTGTAATTACTACAAATTTGAAAAAAAAGGGCAAAACAACCCCATTACATGCAAAATATTTTTTTGTAATTTTATGTTGGTTAAAATTGttt                                                           >  2:2506238/1‑138 (MQ=255)
                                                                                    aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:1889001/140‑1 (MQ=255)
                                                                                    aaaaaaACACGCCTGGTAAACCGGTCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:4432889/140‑1 (MQ=255)
                                                                                    aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:1232772/140‑1 (MQ=255)
                                                                                    aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:2418401/140‑1 (MQ=255)
                                                                                    aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  2:2877029/140‑1 (MQ=255)
                                                                                    aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:1655437/140‑1 (MQ=255)
                                                                                    aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:4173208/140‑1 (MQ=255)
                                                                                     taCAAAAACGCCTGGGAAACCGGGCGTGGTTTTTTTGTTTTTTATTTAATTACTAAAAATTTGAAAAATACGGGAAAAACATGCCATTTACTTAGAAAATATTTGGTTGTAATTTTTTGTAGGTTAAATTTGTAGTTTgg                                                      >  2:7256067/2‑140 (MQ=255)
                                                                                     aacaaacaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGc                                                     <  1:1061493/140‑1 (MQ=255)
                                                                                     aaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATGTAATTAATACAAATTTGAAAAATAAGGGAAAAACAACCCATTTACTTCAAAAATATTTGAAGGTAATTTTATGTAGGTAAAAATTGTATTTggg                                                     >  2:1399777/1‑139 (MQ=255)
                                                                                     aaCAAAAACGCCTGGGAAACCGGGCGTTTGTTTGTTGTTTTTTATTTAAATACAAAAAAATTTAAAAATAAGGGCAAAAACTCCCAGTTACTTAAAAAAAATTTGTTTGTTATTTTATTTAGGTTAAATTTGTtttttgg                                                     >  2:4303607/1‑137 (MQ=255)
                                                                                     aaCAAAAACGCCTGGGAAACCGGGCGTTTGTTTGGTGTTTTTTATTTAATTAATACAAAATTTTAAAATACGGGAAAAAAATACCAGTTAAGTGCAAAAAATTTGTTTGTAATTTTGTGTAGGTAAAAGTTGTATTTGGc                                                     >  2:3791064/1‑140 (MQ=255)
                                                                                     aaCAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTGTAATTACAAAAAATTTTTAAAAAAAGGGAAAAAAAACCCATTTAATTCGAAAATATTTGTTTGTAATTTTATTTGGGTAAAAATTGTTTTTTGc                                                     >  2:428514/1‑140 (MQ=255)
                                                                                         aacaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                                                 <  1:588313/137‑1 (MQ=255)
                                                                                          aaaCGCCTGGGAAACCGGGCGTGTTTTTTGTGTTTTTTATTTAATTACTACAAAATTTAAAAATAACGGAAAAACAACCCAATTTCTTCCAAAACATTTGGTGGTAATTTTTTTTAGGGAAAATTTGTATTTGGTCGtta                                                >  2:198775/1‑139 (MQ=255)
                                                                                            aCGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTTATTAATACAAATTTGAAAAAAAAGGGGAAAAAATACCAATTACTTCCAAAAGATTTTATTGTTATTTTATTTAGGTAAAATTTGTATTTGGGCGTTGag                                              >  2:6487407/1‑140 (MQ=255)
                                                                                              gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGACAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  1:5164815/140‑1 (MQ=255)
                                                                                              gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  2:2051071/140‑1 (MQ=255)
                                                                                              gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  1:6361356/140‑1 (MQ=255)
                                                                                              gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACGTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  1:2786590/140‑1 (MQ=255)
                                                                                              gCCGGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  1:3898294/140‑1 (MQ=255)
                                                                                                      aaCCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaa                                    <  1:7591047/140‑1 (MQ=255)
                                                                                                             cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGGAAAGCggg                             <  1:5759104/140‑1 (MQ=255)
                                                                                                             cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                             <  1:6937126/140‑1 (MQ=255)
                                                                                                             cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                             <  1:5002073/140‑1 (MQ=255)
                                                                                                             cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                             <  2:6608529/140‑1 (MQ=255)
                                                                                                             agTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTACAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                             <  1:7064183/139‑1 (MQ=255)
                                                                                                                   ttttGTTATTTTTTATGTAATGACTACAAATTTGAATAATACGGGAAAAACATCCCATTTACTTCCAAACTATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc                        <  2:4201936/140‑1 (MQ=255)
                                                                                                                      tGTTATTGTTTATGTAATTACTACAAATTTGAAAAATACGGGATAAACATCCCATGTACTTCCAAAATATTAGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTg                     <  2:7698904/140‑1 (MQ=255)
                                                                                                                           ttttttATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAatat                >  2:3092313/1‑140 (MQ=255)
                                                                                                                           ttttttATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAatat                >  2:5942368/1‑140 (MQ=255)
                                                                                                                              ttttATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGTATAttt              >  2:2291933/1‑140 (MQ=255)
                                                                                                                                       aTTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACtt     >  1:3705258/1‑140 (MQ=255)
                                                                                                                                        ttACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACttt    >  1:2485413/1‑140 (MQ=255)
                                                                                                                                        ttACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACttt    >  1:939500/1‑140 (MQ=255)
                                                                                                                                          actacAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACttttt  >  2:5815013/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                            
AGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTT  >  NZ_CP034943/2991115‑2991390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: