BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,219,734	C→A	6.6%	Q358K (CAA→AAA)	oxdC →	oxalate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,219,734	0	C	A	6.6%	813.6 / 10.3	314	Q358K (CAA→AAA)	oxdC	oxalate decarboxylase
Reads supporting (aligned to +/- strand): ref base C (182/98); new base A (2/32); total (184/130)
Fisher's exact test for biased strand distribution p-value = 1.09e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 318 total aligned reads displayed.

TGTTCCATTTGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAG  >  NZ_CP034943/3219598‑3219872

  ttCCATTTGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAg                                                                                                                                       >  1:6826060/1‑140 (MQ=255)

  ttCCATTTGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAg                                                                                                                                       >  2:5623107/1‑140 (MQ=255)

   tCCATTTGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGc                                                                                                                                      >  1:2557729/1‑140 (MQ=255)

     cATTTGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCAt                                                                                                                                    >  2:2373181/1‑140 (MQ=255)

       tttGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCt                                                                                                                                  >  1:981678/1‑140 (MQ=255)

       tttGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCt                                                                                                                                  >  1:1600364/1‑140 (MQ=255)

       tttGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCt                                                                                                                                  >  1:4062882/1‑140 (MQ=255)

       tttGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCt                                                                                                                                  >  1:2417127/1‑140 (MQ=255)

       tttGCAATGGGTCAGTACGTTGAAAACAGCGGTGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTAGGCCGAGGTATCTTTAAACCCATGGCTTGCAATGCTTCCTGAAAGATTAGTTCACCAGCagcg                                                                                                                                  >  1:1630949/1‑137 (MQ=255)

       gttGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCt                                                                                                                                  >  1:1103976/2‑140 (MQ=255)

         tGCAATGGGTCATTACGTTGAAAACATCGGGGATGAACCGCTTGTCTTTTTAGAAATCTTCAAAGACGACCATTATGCTGATGTATCTTTAAACCAATGGCTTGCCATGCTTCCTGAAACATTTGTTCAAGCGCACCTTg                                                                                                                                >  2:6115384/1‑140 (MQ=255)

                ggTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTTATTTAgg                                                                                                                         >  2:1372714/1‑140 (MQ=255)

                 gTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCATTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGa                                                                                                                        <  1:509744/140‑1 (MQ=255)

                  tCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGaa                                                                                                                       <  2:3223183/140‑1 (MQ=255)

                      tACGTTGAAAACTTCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGa                                                                                                                   <  2:340940/140‑1 (MQ=255)

                      tACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGa                                                                                                                   <  2:7119073/140‑1 (MQ=255)

                      tACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGa                                                                                                                   <  2:5407053/140‑1 (MQ=255)

                        cGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACt                                                                                                                 >  1:6740018/1‑140 (MQ=255)

                        cGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACt                                                                                                                 >  1:4944532/1‑140 (MQ=255)

                         gTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACtt                                                                                                                <  2:4796731/140‑1 (MQ=255)

                         gTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACtt                                                                                                                <  1:6525961/140‑1 (MQ=255)

                         gTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACtt                                                                                                                <  2:6596437/140‑1 (MQ=255)

                          ttGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGATAAGACttt                                                                                                               >  1:583975/1‑140 (MQ=255)

                          ttGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACttt                                                                                                               >  2:632883/1‑140 (MQ=255)

                          ttGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACttt                                                                                                               >  1:537155/1‑140 (MQ=255)

                          ttGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTGGCAATGCTTCCTGAAACATTAGTTCCACAGCATCTTGATTTAGGAAAAGACttt                                                                                                               >  1:4851563/1‑140 (MQ=255)

                           tGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTa                                                                                                              >  2:3892914/1‑140 (MQ=255)

                            gAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTAc                                                                                                             >  2:4961163/1‑140 (MQ=255)

                             aaaaCATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACt                                                                                                            >  1:5633223/1‑140 (MQ=255)

                                  aTCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCGGATGTATCTTTGAACCAATGGCTGGCAATGCTTCCTGAAACAGTAGTTCTACAGCATCTTGATGTAGGACAAGAGTTTACTGctat                                                                                                       >  1:4194901/1‑140 (MQ=255)

                                  aTCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGTCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGatat                                                                                                       >  1:7601678/1‑140 (MQ=255)

                                  aTCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGatat                                                                                                       >  1:4581022/1‑140 (MQ=255)

                                  aTCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCAGTATGCCGATGTATCTTTAAACCAATTGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCAGCTTGATTTAGGCAAAGACTTTAGTGatat                                                                                                       >  2:6269174/1‑140 (MQ=255)

                                    cGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTc                                                                                                     >  1:6181759/1‑140 (MQ=255)

                                    cGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTc                                                                                                     >  1:3296257/1‑140 (MQ=255)

                                       ggACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCttt                                                                                                  >  2:1881340/1‑140 (MQ=255)

                                       ggACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCttt                                                                                                  <  1:6390432/140‑1 (MQ=255)

                                            aaCCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCgaaa                                                                                             <  1:1715611/140‑1 (MQ=255)

                                                 cTTGTCTTTTTAGAAATCTATAAAGACGATCATTATGCCGATGTATCTTGAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGaaaa                                                                                        <  1:7458664/140‑1 (MQ=255)

                                                   tGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGc                                                                                      >  1:4706342/1‑140 (MQ=255)

                                                         tttAGAAAGCTTTAAAGACGATCATAATGCCGATGTATCTATAAAACAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCgg                                                                                <  2:7070147/140‑1 (MQ=255)

                                                                      aaaGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGaa                                                                   <  1:7588668/140‑1 (MQ=255)

                                                                           cGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCa                                                              <  1:2297515/140‑1 (MQ=255)

                                                                            gATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAg                                                             >  1:2727537/1‑140 (MQ=255)

                                                                             aTCATTATGCCGATGTATCTTTAAACCAATGGGTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGt                                                            >  1:3604670/1‑140 (MQ=255)

                                                                             aTCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGt                                                            >  1:7181461/1‑140 (MQ=255)

                                                                             aTCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGt                                                            >  2:7445550/1‑140 (MQ=255)

                                                                             aTCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCAGCCGGTTGTAAAAAAGAAATGCAGt                                                            >  1:4655240/1‑140 (MQ=255)

                                                                                      ccGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGACTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTACAAAAGAAATGCAGTAAATaaaaa                                                   >  2:854915/1‑140 (MQ=255)

                                                                                         aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACg                                                >  2:5450834/1‑140 (MQ=255)

                                                                                         aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACg                                                >  2:7198118/1‑140 (MQ=255)

                                                                                          tttttCTTTAAAAAATTGCCTTGAATTTCTTCCTTAAACTTTATTTCAACTGCATTTTTATTTTGTAAATGATTTTACTGATATTTTTTCGAAAGAAACGCATCCGGTTTTAAAAAAGAAATGCAGTAAATAAAAAACGa                                               <  2:7613510/136‑1 (MQ=255)

                                                                                          tttATTTTTAAACCAATGGTTTGAATTGATTCCTTAAACTTTAGTTCAACAGATTATTTTTTTAGTAAAAGATTTTAGTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCATTAAATAAAAAACGa                                               <  2:4457874/138‑1 (MQ=255)

                                                                                          tGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAActa                                               >  1:6268620/1‑138 (MQ=255)

                                                                                          tGTATATTTAAAACAATGGCTGTCATTCTGTCCTCAAAATTTATTGCAACACAATCTTGATTTAGCAAACGACTTTACTGATATTCTTTCGAAAAAAAAGCATCCGTTTGAAAAAAATAAAGGCTGTAAATAAAACACGa                                               <  2:6775220/140‑1 (MQ=255)

                                                                                              tCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAATAAATGCAGTAAATAAAAAACGACCAg                                           >  2:3045387/1‑140 (MQ=255)

                                                                                              tCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAg                                           >  2:2591876/1‑140 (MQ=255)

                                                                                              tCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAg                                           >  2:4616241/1‑140 (MQ=255)

                                                                                               atttAAACCAATGGCTTGCAATGCTGCCTTAAACTTTTGTTAAACATCATTTTTTTTTAGAAAAATACTTTACTTATTTTCTTTCCAAACAAACGCATCGGGTTTAAAAAAATAAATGAATTAAATAAAAAACGACCAGc                                          <  2:2120707/139‑1 (MQ=255)

                                                                                                  tAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAACGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTg                                       >  1:877785/1‑140 (MQ=255)

                                                                                                    accccctGGCTTCAAAGGCTTCCTCAAACATTATTACCACACCATTTTGATTGAGGAAAAGACTTTACTCATATTCTTACGAACCAAAAGCACCCGTTTTTAAAAACTAAATGCCGAAAATAAAAAACGACCAGCTTGCa                                     <  2:3095479/134‑1 (MQ=255)

                                                                                                    aaCCATTGGCTTTAAATTCTTCCTTAAACTTTATTAAAAAAGAATCTTGATTTAGTAAAATACTTTACTCATATTCTTCAGAATCAAAACCATCGGGTTTTAAAAAACAAATCAATTAAATAAAAAACGACCAGCTTGCa                                     <  2:2759606/140‑1 (MQ=255)

                                                                                                    aaCCATTGCCTTGCAATGCTTCCTCAAACATTATGTCAACACCATCTTTATTGAGCAAAAGACTTTACTGATATCGTTCCGAAACAAAAGCATCCGGTTGTAAAAAAGAAATCCACTAATTAAAAAACGACCAGCTTGCa                                     <  2:5082571/140‑1 (MQ=255)

                                                                                                    aaCCAATGGCTTGCAATGCTTCCTCAAACATTATTTAAACAGCTTTTTTTTTTAGTAAATTATTTTACTTATATTCTTTCTAAACAAAAGCATCGGGTTGTAAAAAACAAATCAAGTAAATAAAACACGACCAGCTTGCa                                     <  2:2558785/140‑1 (MQ=255)

                                                                                                    aaCCAATGGCTTCCATTGCTTCATGAAAAATTAGTACAACAGCATATTGATTTACGAAAAGACTTTACTGATATTCTTTCCAAAGAAAAGCATCCGGTTGTAAAAAATAAATGCAGTAAATAAAACACGACCAGCTTGCa                                     <  2:3141564/140‑1 (MQ=255)

                                                                                                         aTGGCTTGCAATGCTTCCTGAAACCTTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagagag                                >  2:1658267/1‑140 (MQ=255)

                                                                                                         aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACTACCAGCTTGCagagag                                >  2:3788794/1‑140 (MQ=255)

                                                                                                         aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagctgt                                >  1:3480020/1‑136 (MQ=255)

                                                                                                         aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagagag                                >  2:4002888/1‑140 (MQ=255)

                                                                                                         aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagagag                                >  1:1514977/1‑140 (MQ=255)

                                                                                                         aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagagag                                >  2:6581326/1‑140 (MQ=255)

                                                                                                         aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagagag                                >  2:3696455/1‑140 (MQ=255)

                                                                                                         aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagagag                                >  1:2941127/1‑140 (MQ=255)

                                                                                                         aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagagag                                >  1:5444185/1‑140 (MQ=255)

                                                                                                         aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagagag                                >  2:769356/1‑140 (MQ=255)

                                                                                                         aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCagagag                                >  2:2062475/1‑140 (MQ=255)

                                                                                                         aTGGCTTGCAATGCTTCCTGAAAAATTATTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTAGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACAAGCTTGCagagag                                >  2:4553447/1‑140 (MQ=255)

                                                                                                          tGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTCGGAAAAGACTTTACTGATATTCTTTCGCAAGAAAAGCATCCGGTTGTAAAAAAGACATGCAGTAAATCAAAAACGACCAGCTTGCAGAGAGc                               >  2:6412785/1‑140 (MQ=255)

                                                                                                          tGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGc                               >  1:7103183/1‑140 (MQ=255)

                                                                                                          tGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAAAGACCAGCTTGCAGAGAGc                               >  2:1788237/1‑140 (MQ=255)

                                                                                                                 cAATCCTCCCTCAACAATTACTTCCACCCCATCTTGATTGAGGAAAAGACTTGAGTGATAGTCTGTCGAAAGAAACGCAGCCGGTGTTAAAAAACAAAGGCAGTAAATAAAAACCGACCAGCTTGCAGAGAGCAATCGtt                        <  2:5164554/140‑1 (MQ=255)

                                                                                                                      cTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTg                   >  2:4840003/1‑140 (MQ=255)

                                                                                                                      cTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTg                   >  1:2324434/1‑140 (MQ=255)

                                                                                                                      cTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTg                   >  1:3764215/1‑140 (MQ=255)

                                                                                                                      cTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTg                   >  2:3533084/1‑140 (MQ=255)

                                                                                                                       ttCCTCAAACTTTATTTCAACATCATTTTTATTTATTAAAATATTTTACTTTTATTCTTTCAAAACAAACGCATCGGGTTTTAAAAAAAAAATCCATAAAAAAAACCACGACCAGCTTGCAGAGAGCAATCGTTCTCTGc                  <  1:4553447/140‑1 (MQ=255)

                                                                                                                        tccataaaTATTAGTTCAACAGAATCTTTATTTAGCAAAATACTTTACTGATATTATTTCTAAAGAAAAGAATCCGGTTGTAAAAAAGAAATGCCTTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCa                 <  2:4581022/135‑1 (MQ=255)

                                                                                                                        tcattaaaCATTAGTTAAAAACCATGTTTATTTAGGAAAAATGTTTACTGAAATTCTTTCGAAAGAAAACCTTCCGGTTGTAAAAAAGAAATGCTGTAAATAAACCACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCa                 <  2:5926351/135‑1 (MQ=255)

                                                                                                                        tCCTGAAACATTAGTTCAACCGCATCTTGATTTTGTAAAAGACTTTACTGATTTGGTTTCGAAAGAAAAGCATCCGTTTGTAAAAAATAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCa                 <  2:7002406/140‑1 (MQ=255)

                                                                                                                        tCCTCATACATTAGTTAAACATCATCTTTATTTAGTAAAAGATTTTACTGATATTCTTTCGAAAGAAAAGCATCCTGTTTTAAAAAATAAATGAAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCa                 <  2:7571480/140‑1 (MQ=255)

                                                                                                                          cTGAAACATTAGTTCAACAGAATCTTTATTTAGTAAAAGACTTTACTGATATTCTTTCCAAAGAAAAGCATCCGTTTGTAAAAAAGAAATGCAGTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAg               <  2:5739295/140‑1 (MQ=255)

                                                                                                                           ttaaaCATTAGTTCAACAGCATCTTTATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGAATCCGGTTTTAAAAAAGAAATCCATTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGc              <  2:4194901/138‑1 (MQ=255)

                                                                                                                             aaaCATTAGTTAAAAAGAATCTTTATTTAGTAAAATATTTTACTTATTTTCTTTCTAATGAAAAGAATCGGTTTTTAAAAAATAATTGCAGTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCt            <  2:1201053/140‑1 (MQ=255)

                                                                                                                                cATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCGTTta         >  1:1054316/1‑140 (MQ=255)

                                                                                                                                  ttcgtacAACAGAACCTTGTTTTAGAAAAACAATTTACTCATATTCTTTCCAAAGAAACGCATCGGTTTTTAAACAAGAACTGCACAAAATAAACCACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTtata       <  1:961841/134‑1 (MQ=255)

                                                                                                                                  ttATTTCAACAGCATTTTTTTTTATTAAAATATTTTACTTATTTTTTTTCGAAATAAAATCAGCGGTTTTTAAAAAATAAAGGCATTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTtata       <  1:4455200/140‑1 (MQ=255)

                                                                                                                                  ttAGTACAACAGCATCTTTTTTTAGTAAAATACTTTACTTATATTATTTATAAATAAAAGCATCGGTTTTTAAAAAAGAAATCCAGTAAAAAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTtata       <  1:7757509/140‑1 (MQ=255)

                                                                                                                                   tAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATaa      >  2:4360940/1‑140 (MQ=255)

                                                                                                                                   tAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATaa      >  2:6889082/1‑140 (MQ=255)

                                                                                                                                     gtacAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAgg    <  2:1608411/137‑1 (MQ=255)

TGTTCCATTTGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCAGTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAG  >  NZ_CP034943/3219598‑3219872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`