BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,280,377	G→T	8.9%	Q115K (CAA→AAA)	EO946_RS16965 ←	lantibiotic dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,280,377	0	G	T	8.9%	1071.7 / 11.9	446	Q115K (CAA→AAA)	EO946_RS16965	lantibiotic dehydratase
Reads supporting (aligned to +/- strand): ref base G (253/125); new base T (2/65); total (255/191)
Fisher's exact test for biased strand distribution p-value = 5.10e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 446 total aligned reads displayed.

AGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCTTTTTTTTCCCTTT  >  NZ_CP034943/3280239‑3280515

 gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                                                          >  1:455887/1‑140 (MQ=255)

 gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                                                          <  1:1312977/140‑1 (MQ=255)

 gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                                                          >  2:6679942/1‑140 (MQ=255)

 gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                                                          <  1:6934952/140‑1 (MQ=255)

 gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                                                          <  1:2398868/140‑1 (MQ=255)

 gTAAGCCCGGTCACCATTAATATAACAAGCGTAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                                                          <  1:2285126/140‑1 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTACAATGACTGTTGAGTa                                                                                                                                        >  2:6434156/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATTAATGTTGATTa                                                                                                                                        >  1:4924943/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                        >  1:6770992/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                        <  2:591162/140‑1 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                        >  1:3114279/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                        >  1:7502403/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                        >  2:7445026/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCAAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                        >  1:3583132/1‑140 (MQ=255)

      cccGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTaaaa                                                                                                                                     >  1:322274/1‑140 (MQ=255)

       ccGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAg                                                                                                                                    <  1:6214187/140‑1 (MQ=255)

          gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAtata                                                                                                                                 >  1:2988234/1‑140 (MQ=255)

          gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAAGTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGACTGTTGATTAAAAGATa                                                                                                                                 >  2:2481562/1‑140 (MQ=255)

           tCACCCTTAATATAACAAGCGGACTTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTGTTGTAAAATGAATGTTGATTAAGAGATaa                                                                                                                                >  2:5698161/1‑140 (MQ=255)

           tCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATaa                                                                                                                                >  2:1293877/1‑140 (MQ=255)

            cACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAg                                                                                                                               <  1:2545539/140‑1 (MQ=255)

            cACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAg                                                                                                                               <  1:7741153/140‑1 (MQ=255)

                      ataACCCGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAAGCAACACGAGCCTTTTTGTAAAATGAATGTGGATTAAAAGATAAGTGATTTTTAt                                                                                                                     <  1:5031740/140‑1 (MQ=255)

                      ataACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                                                                                     <  2:3366809/140‑1 (MQ=255)

                      ataACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                                                                                     <  2:1484896/140‑1 (MQ=255)

                      ataACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                                                                                     <  1:3507066/140‑1 (MQ=255)

                      ataACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                                                                                     <  2:3628336/140‑1 (MQ=255)

                      ataACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                                                                                     <  2:3532174/140‑1 (MQ=255)

                      ataACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAAGTTTCTAATTAATTGGTAGAGCCACTCAAAATCACCACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                                                                                     <  2:377832/140‑1 (MQ=255)

                      ataACAAGCCGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                                                                                     <  2:4457281/140‑1 (MQ=255)

                         aCAAGCGGGATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                  >  1:6958904/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCa                                                                                                                   >  1:2699334/1‑139 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCa                                                                                                                   >  1:5669033/1‑139 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                  >  2:1782589/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                  >  1:1174190/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                  >  1:6526112/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                  >  2:7583604/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                  <  2:7306410/140‑1 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                  >  2:695478/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                  >  1:4135119/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                  >  1:3862357/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAGTAAAAGATACGTGATTTTTATCAg                                                                                                                  >  2:2849161/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTGGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                  >  1:6024365/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATAAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                  >  2:6736145/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTACAATTTTCTAATTAATTGTTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAATTGATTTTTATCAg                                                                                                                  >  2:1026442/1‑140 (MQ=255)

                             gggtattttATTGTAAAATAATGTGGGTGGGTGTATTCGTTTTCCTATTTACTATTTATTTGGTAGAGCCACTCAAAATCAACACGAGCATTTTTGTAAAATTAATGTTGATTAAAACATAAGTTATTTTTATCAGaaaa                                                                                                              <  2:4588346/134‑1 (MQ=255)

                             gcttaaTTTATTTTAAAATAAATTCGGTCGGTGTATTCTTTTTCAATTTTTCTAATTATTTGTTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATTATTGTTTTTTAAAATATAAGTTATTTTTATCAGaaaa                                                                                                              <  2:2681772/136‑1 (MQ=255)

                             gcgtattttAATGTAAAATAAAGTGGGTGGGTTTTTTCTTTTTCCAATTTTTTATTTATTTGGTAGAGCCACAAAAAATCAACACGAGCCTTTTTTTAAAATGAATTTTTATTAAAAGTTAAGTTATTTTTATAACaaaa                                                                                                              <  2:3961153/134‑1 (MQ=255)

                             gcgcAATTTAAGGTAAACGAAAGTCTGAGGGTTTTTTCGTTTTCCATTTTTATATTTATTTGGTTGAGCCACTCAAAATCAACACGAGCCTTTTTTTAAAATGATTGTTGATTAAACGATAAGGGTTTTTTATCAGaaaa                                                                                                              <  2:4127069/140‑1 (MQ=255)

                             gCGGAATTTATTGTAAAAGAAAGTGGGTCGGTGTTTTGGTTTTCCTATTTTCTAATTATTTGGTAGAGCAACTCAAAATCAACCCGAGCCTTTTTGTAAAATGATTGTTTATTAAAAGATATGTTATTTTTATCAGaaaa                                                                                                              <  2:3083836/140‑1 (MQ=255)

                             gCGGAATTTAATTAAAAAGATTGTCGGTCGGTGTTTTCTTTTTCCATTTTTATAATTATTTGTTTGAGCCACTCAAAATAAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAACATAAGTTATTTTTATCAGaaaa                                                                                                              <  2:3894182/140‑1 (MQ=255)

                             gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTATAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                              >  1:5575558/1‑140 (MQ=255)

                             gCGGAATTTAATGTAAAACAAAGTCGGTGGGTGTATTCGTTTTCCAATTTTCTAATTATTTGGTAGAGCAACTAAAAATAAACACGACCCTTTTTGTAAAATGAATTTTGATTAAAACATAAGTGTTTTTTATCAGaaaa                                                                                                              <  2:5141755/140‑1 (MQ=255)

                             ccgcaaTTTAATGTAAAAGAAAGTCGGTCGTTTTATTCGTTTTCCAATTTTATAATTAATTGGTCGAGCACCTAAAAATCAACACGAGCCTTTTTTTAAAATGAATGTTGATTAAAACATAAGTGATTTTTATCATaaaa                                                                                                              <  2:4393878/136‑1 (MQ=255)

                             cagtaaTTTAATGTAAAAGAAAGTCGGTCGGTGTATTCTTTTTCCAATTTTCTAATTATTTGGTAGAGCAAATAAAAATCAACACGCGCTTTTTTGTAAAATGATTGTTGATTAAAAGATAATTGATTTTTATCAGaaaa                                                                                                              <  2:5210021/136‑1 (MQ=255)

                               ttaatttaatttAAAATAAATTGGGTGGGTTTTTTCTTTTTCCATTTTTCTAATTATTTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCATAAAACg                                                                                                            <  2:5032568/138‑1 (MQ=255)

                                  aTTTAATGTAAAATCAAGTCGGTCTGTGTATTCGTTTACCAATTTTCTAATTATTTGTTAGAGCCACTCAAAAGCAACACGCGCCTTTTTGTAAAATGACTGTTTATTAAAACATAAGTGATTTTTATCAGAAAACGAcc                                                                                                         <  2:6075151/140‑1 (MQ=255)

                                      aaTGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGAGATTTTTATAATAAAACGACCCtat                                                                                                     >  2:7176199/1‑140 (MQ=255)

                                                    tcggtcggTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGaaa                                                                                       >  2:7690865/1‑140 (MQ=255)

                                                      tgttgttgtATTCTTTTTCCATTTTTATAATTAATTGGTAGAGCCACTAAAAATCAACACGACCGTTTTTTTAAAATTATTTTTTATTAAAAGTTAATTGTTTTTTATCAGAAAACGACCCTATACCGACTGAAGaaaaa                                                                                     <  1:5356989/134‑1 (MQ=255)

                                                      ggtcgttTTATTCTTTTTCAAATTTTATAATTATTTGTAACACCCACAAAAAAAAAACACCAGCATTTTTTTAAAATTAATTTTTATAAAAACATAAGTGATTTTTAACACAAAACGACCCTATACCGACTGAAGaaaaa                                                                                     <  1:5759864/140‑1 (MQ=255)

                                                       gtcggtGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGaaaaaa                                                                                    >  1:1592615/1‑140 (MQ=255)

                                                            ttttttCTTTTTCCAATTTTCTAATTAATTTTTAGAGCAACTCAAAATCAAAACGAGCCTTTTTGTAAAATGAATGTTTATTAAAAGTTAAGTGATTTTTATATGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCa                                                                               <  2:5609314/136‑1 (MQ=255)

                                                             ttATTCTTTTACCAATTTTATAATTATTTGTTACACACACACAAAAACACCACGCCCCTTTTTTAAAAATTAATTTTTTTTAAAAGATACTTTATTTTTATCACAAAACGACCCTATACCGACTGAAGAAAAAAGGCCaa                                                                              <  1:6314813/139‑1 (MQ=255)

                                                              ttttCTTTTTAAAATTTTATAATTATTTGTTAGAGCAACTCAAAATAAACACGAGATTTTTTTTAAAATGAATTTTTATAAAAATATATTTTTTTTTTATAAAAACACGACCCTATACCGACTGAAGAAAAAAGGCCaaa                                                                             <  1:5940675/138‑1 (MQ=255)

                                                              atttgtttttCCATTTTTCTATTTAATGGGTAGACCAAATAAAAATCAACCCGAGCCTTTTTGTAAAATGATTGTTGATAAAAACATACGTTATTTTTATCATAAAACGACCCTATACCGAATGAAGAAAAAAGGCCaaa                                                                             <  1:3697027/134‑1 (MQ=255)

                                                                  tgTTTTCCAATTTTCTATTTATTTGGTAGAGCAAATCAAAAACAACACGAGCCTTTTTTTAAAATTATTGTTGATTAAAATATAAGTTTTTTTTAAAAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGgt                                                                         <  1:1862521/139‑1 (MQ=255)

                                                                      ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTCTACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                     >  2:6363153/1‑140 (MQ=255)

                                                                      ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                     >  1:1008399/1‑140 (MQ=255)

                                                                      ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                     >  1:4729007/1‑140 (MQ=255)

                                                                      ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                     >  2:1662187/1‑140 (MQ=255)

                                                                      ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                     >  2:5543362/1‑140 (MQ=255)

                                                                      ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                     >  1:7393351/1‑140 (MQ=255)

                                                                      ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATAAGAAAACGACCCTATACCGACTGAACAAAAAAGGCCAAAAGGGGTgg                                                                     >  2:6221304/1‑140 (MQ=255)

                                                                        ccAATTTTCTAATTAATTTGTATATACACTCAAAATAAAAAATAGAATTTTTGTAAAATTAATGTTTATTAAAAGATAAGTGATTTTTATCAGAAAAAGACCCTATACCGACTGAAGAAAAAATGACAAAAggctgtctc                                                                   >  2:6547343/1‑133 (MQ=255)

                                                                        ccAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGTCCAAAAGGTGTGGtt                                                                   >  2:5074093/1‑140 (MQ=255)

                                                                             tttCTAATTAATTGGTAGAGCCACACAAAATCAACACCAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAACAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCttgt                                                              <  2:20799/140‑1 (MQ=255)

                                                                             ttgcTAATTAATGGGTAGAGCCCCTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAACAGATAAGTGTTTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCttgt                                                              <  2:1200681/137‑1 (MQ=255)

                                                                                   attaattGTTAGAGCCACTCAAAATCAACACGCGCCTTTTTTTAAACTGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGctct                                                        <  2:5400105/140‑1 (MQ=255)

                                                                                   ataaattGGTAGCGCCACACAAAATCAACACGAGCGTTTTTGTAAAATCAATGTTGATTAAAAGATACGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGctct                                                        <  2:5813501/137‑1 (MQ=255)

                                                                                     tatttGGTAGCGCCACACAAAATAAACACGAGCCTTTTTGTAAAAGGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAACACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCtcttc                                                      <  2:7630619/137‑1 (MQ=255)

                                                                                     taattGGTATAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATTAATGTTGATTAAAAGATACGTTATTTTTATCACAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCtcttc                                                      <  2:3025502/140‑1 (MQ=255)

                                                                                       attGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTcttctt                                                    >  2:235172/1‑140 (MQ=255)

                                                                                             cgcgCCACTCAAAATCAACACGAGCTTTTTTGTAAAATCAATGTTGATAAAAAGATAAGTGATTTTTATCACAAACCGACCCTATACCGACTGAAGAAAAAAGGCCAAACGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                              <  1:7195148/137‑1 (MQ=255)

                                                                                             agagCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                              >  2:1898139/1‑140 (MQ=255)

                                                                                             agagCCACAAAAAATCACCCCGAGCCTTTTTGTAAAATGATGGTTTATTAAAAGATACGGGATTTTTATCACAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                              <  1:854953/140‑1 (MQ=255)

                                                                                             agacCCACTAAAAATCAACACGACCCTTTTTTTAAAATGATTGTTTATTAAAAGATAAGTTATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                              <  1:3909288/140‑1 (MQ=255)

                                                                                             agacCCACTAAAAACCAACACGAGCCTTTTTGTAAAATGATTGTTTATTAAAAGATAAGTGATTTTTAACACAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                              <  1:3371603/140‑1 (MQ=255)

                                                                                              gagCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCAt                                             >  1:509555/1‑140 (MQ=255)

                                                                                                    ctaaaaaTCAACACGAGCCTTTTTTTAAAATGAATGTTTATTAAAAGATATGTGATTTTTATCATAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCa                                       <  2:2566466/137‑1 (MQ=255)

                                                                                                     tCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCaa                                      >  2:548338/1‑140 (MQ=255)

                                                                                                           taaaCACGAGCATTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCATAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGc                                <  2:4173318/138‑1 (MQ=255)

                                                                                                           caaaCACCCGCTTTTTTTTAAAATGAATGTTGATTAAAAGATATGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGc                                <  2:4729007/138‑1 (MQ=255)

                                                                                                               cacGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGt                            >  1:2144412/1‑140 (MQ=255)

                                                                                                               cacGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGt                            >  2:2166872/1‑140 (MQ=255)

                                                                                                                         ttttTAAAATTAATTTTTATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTgc                  <  2:6894617/140‑1 (MQ=255)

                                                                                                                          ttGTAAAATGAATGTTGATTAAAAGATACGTGAGTTTTATCCGAACACGACCCTATACCGACTGAAGAAAAAAGGCCAAACGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTgcg                 <  1:1678596/140‑1 (MQ=255)

                                                                                                                                aattaaggTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTGCTTGTTGCGCTTCTTGTTGCATACTTCAAAATAGCCTGGTGACAGTTGCGCtttgt           >  2:493767/1‑138 (MQ=255)

                                                                                                                                aatgaatgTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCttttt           >  2:4783207/1‑140 (MQ=255)

                                                                                                                                         tGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCTTTTTTTTCCCttt  >  1:4376777/1‑140 (MQ=255)

AGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCTTTTTTTTCCCTTT  >  NZ_CP034943/3280239‑3280515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`