BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,700	A→C	12.2%	E181D (GAA→GAC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,700	0	A	C	12.2%	293.7 / 20.8	151	E181D (GAA→GAC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (80/49); new base C (15/7); total (95/56)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

ACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAC  >  NZ_CP034943/607566‑607837

aCGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCgg                                                                                                                                      <  1:3698218/140‑1 (MQ=255)

 cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                                                     <  1:974904/140‑1 (MQ=255)

 cGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                                                     <  1:1853876/140‑1 (MQ=255)

  gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACAGGGGAAGCTGGCGCAGGCGGCGCTACCGGAGCTAACGGGGCAGCgggcg                                                                                                                                    >  2:2146477/1‑138 (MQ=11)

           aGGCGCTACTGGGGAAGCCGCACCAGCCGGAGCGACAGGAGCGACCGGGGTAGCCGGACCAGCCGGAGCGACAGGCGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                                                                                           <  2:3825418/140‑1 (MQ=14)

            ggggCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGc                                                                                                                          >  1:2519051/1‑140 (MQ=255)

               gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                       <  2:613331/140‑1 (MQ=40)

                       ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGcga                                                                                                               >  2:3067916/1‑138 (MQ=255)

                       ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACCGGAGcga                                                                                                               >  2:242833/1‑138 (MQ=255)

                        gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGCAGCTAc                                                                                                              >  1:1301019/1‑140 (MQ=255)

                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCAACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCAAACGCCGACCCCGGAACAGCCCTAGCTACAGGAGCTAcc                                                                                                             >  1:2489602/1‑140 (MQ=14)

                         aaGCCGGACCAGCCGGAGCGACAGGAGATACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                                                             >  1:454531/1‑140 (MQ=255)

                             cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCGGGCGCACGCGGAGCGACAGCAGCGACAGGCGAAGCCGGGGACGCCGGAGCGACAGGTGCTACCgggg                                                                                                         >  2:2610470/1‑140 (MQ=14)

                              ggACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGa                                                                                                        >  2:479282/1‑140 (MQ=255)

                                aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                      >  2:598277/1‑140 (MQ=255)

                                    gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGTCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                                                                                                  >  2:1518679/1‑140 (MQ=255)

                                    cccGGAGGCACAGGAGCTCCCGGCCTCGCCGGAGCAACCGGAGCCACAGGAGCTCCCGGCGAACCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCGGGAGCGACAGGAGCTACCGGGGAAGCCgg                                                                                                  <  2:3034580/139‑1 (MQ=11)

                                     ccGGAGCGACGGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGGGAAGCCGGACCAACCGCAGCGACAGGAGCTACAGGGGAAGCCGGACCAGCGGGAGCGCCCGGAGCGAGCGGGGACGCgggc                                                                                                 >  1:416421/1‑139 (MQ=14)

                                     ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGACGCCGGACCAACCGGAGCGACAGGAGCGCCCGGCGCCGCAGGACCAGCCGGAGCGCCAGGAGCTCCCGGCGCAGCCGGa                                                                                                 >  2:1524406/1‑140 (MQ=14)

                                          gacaCAGGAGCTCCCGGCGAACCCGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                                                                                            <  1:931312/137‑1 (MQ=11)

                                          gCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                                                                                            <  1:2536939/140‑1 (MQ=255)

                                                      aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGGAGCCGGACCAACCGGAGCGACggg                                                                                >  2:692814/1‑140 (MQ=255)

                                                      aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                >  2:1093604/1‑140 (MQ=255)

                                                      aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGACGCCGGCCCAGCCGGAGCGACGGGAGCGACCGGGGCAGCCGGACCAACCGGAGCGACggc                                                                                >  2:1005885/1‑139 (MQ=14)

                                                       ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGAGCGACCGGAGCTACCGGGGAAGCCGGGCCCGCCGGAGCGACAGGAGCTCCCGGGGAGACCGGACCAGCCGGAGCGCCgggc                                                                               >  2:823629/1‑139 (MQ=11)

                                                        cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACGGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAg                                                                              <  1:183499/140‑1 (MQ=255)

                                                        cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAg                                                                              <  1:1093604/140‑1 (MQ=40)

                                                            gacgCCGGCGCACCCGGAGCGCCAGGAGCTACCGGCGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                          <  2:3102099/137‑1 (MQ=14)

                                                            gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCCGGAGCGCCCGCGGACGCCGGAGCACCCGGAGCGCCGGGAGcgcc                                                                          >  2:3084676/1‑137 (MQ=11)

                                                            ccatctggATCAGCCCGAGCCACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                          <  1:3037817/134‑1 (MQ=14)

                                                               gCCGGACGACCCGGCGCCACAGGAGCTACCGGGGAAGCCGGACCACCCGTCGCGAGTGGAGCTTCCTGCTAAGCCGGCCCTGCCGGAGCTACAGGTGCTCCCGGCGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                       <  1:1428000/140‑1 (MQ=9)

                                                               gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                       <  1:1314443/140‑1 (MQ=255)

                                                               cccGGACCAGCTGGTGCTCCCGGAGCTACCGGGGAAGCCGGAACAACCTGAGCTACCGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                       <  1:1668208/139‑1 (MQ=14)

                                                                 cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                     >  2:3676551/1‑140 (MQ=255)

                                                                 cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACgggag                                                                     >  2:2848612/1‑138 (MQ=255)

                                                                 cGGACCACCCGGACCCACAGGAGCCACAGGCGCCCCGGCACCAACCGGAGCGACAGGACCAACCGGCGCAGCCGGACCCCCGGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                                                     <  1:674764/140‑1 (MQ=9)

                                                                    aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa                                                                  >  1:328312/1‑140 (MQ=255)

                                                                    aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa                                                                  >  1:2677278/1‑140 (MQ=255)

                                                                        gCCGGAGCGACCGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTgg                                                              <  2:3576785/140‑1 (MQ=255)

                                                                        gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTgg                                                              <  2:2519649/140‑1 (MQ=255)

                                                                        cccGGAGCGACAGGCGCTACCGGGGAAGCCGGACCACCCGGAGCGACTGGAGCTACCGGGGAAGCCGCACAATCCGGAGCGACAGGAGCTCGCGGGGAAGCCGGACCAACCGCAGCGACGGGAGCCACAGGGGCTACTgg                                                              <  2:2519051/139‑1 (MQ=14)

                                                                         ccGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTggg                                                             >  1:2217019/1‑140 (MQ=255)

                                                                           ggAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGa                                                           <  1:3098430/140‑1 (MQ=255)

                                                                            gAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGAc                                                          <  1:598277/140‑1 (MQ=255)

                                                                             aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                         >  1:1074543/1‑140 (MQ=255)

                                                                                   aggaggTACCGGTGAAGCCGGACCAACCGGTGCAACTGGAGCACCCGGGGAACCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                                   <  1:3325843/140‑1 (MQ=18)

                                                                                   aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                         >  1:373685/1‑118 (MQ=255)

                                                                                   aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                         <  2:373685/118‑1 (MQ=255)

                                                                                   aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                         <  2:3753851/118‑1 (MQ=255)

                                                                                   aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                                   <  2:2677278/140‑1 (MQ=255)

                                                                                       gCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                <  1:326845/107‑1 (MQ=40)

                                                                                       gCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                >  2:326845/1‑107 (MQ=40)

                                                                                        cgaCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGcc                                              <  1:3504550/138‑1 (MQ=255)

                                                                                          aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCAACTGGCGACGGCGGACCGGCggg                                            >  2:1664471/1‑140 (MQ=17)

                                                                                          aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                            >  1:2078315/1‑140 (MQ=255)

                                                                                          aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCAACGGGGGACGTCGGACCAGCCgg                                            >  1:2443688/1‑140 (MQ=18)

                                                                                           ccGGGGAAGCCGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTCCTGGGTAACTCGGACCAGCCGGa                                           >  1:211364/1‑140 (MQ=17)

                                                                                           ccGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGCCAAGTCGGACCAGCCGGa                                           >  1:3465801/1‑140 (MQ=17)

                                                                                           ccGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                         >  2:3109532/1‑110 (MQ=255)

                                                                                            cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCCGGAGCCACAGGCGCATCGGGAGAAAACGGAGCTCCGGGAGCCAAAGCCGCTACCGGGGACGCCGGACCAACCGGAg                                          >  1:3311201/1‑140 (MQ=2)

                                                                                            cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                       >  2:2382623/1‑63 (MQ=37)

                                                                                            cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCCGGAGCCACAGGGGCGACGGGGGACGGCGGACCAGCCGGAg                                          >  2:3399297/1‑140 (MQ=17)

                                                                                            cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAg                                          <  1:1518679/140‑1 (MQ=255)

                                                                                            cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCGGGAGCCACAGGGGCGACTGGGGACGTCGGACCTGCGGGAg                                          >  1:3776776/1‑140 (MQ=255)

                                                                                            cGGGGAACCCGGACCAACCGGAGCGACAGGAGCGCCCGGGGAACCCGGCCCAGCCGGAGCGACAGGAGCAACCGGCGCAGCCGGACCAACCGGAGCCACCGGAGCCACAGGCGCTACCGGGGACGGCGGACCAGCCGCAg                                          >  2:2499558/1‑140 (MQ=17)

                                                                                                gAAGCCGGCCCAACCGGAGCCACAGGAGCTCCCGGCGACGCCGGACCAGCCGCAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAGGGGAGCCACAGGGGCTACTGGGGTCGTCGGACCAGCCGGAGCGAc                                      <  2:3824466/140‑1 (MQ=18)

                                                                                                gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACGTCGGACCAGCCGGAGCGAc                                      >  1:3066885/1‑140 (MQ=255)

                                                                                                gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                      >  1:1883413/1‑140 (MQ=255)

                                                                                                   gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTCCTGGGGACGTCGGCCCAGCCGGAGCGACAgg                                   >  1:43903/1‑140 (MQ=255)

                                                                                                   gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGACGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACAAGACGGAGCGACAgg                                   >  1:321159/1‑140 (MQ=255)

                                                                                                     cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAg                                 >  2:1989038/1‑140 (MQ=255)

                                                                                                     cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAg                                 >  2:122941/1‑140 (MQ=255)

                                                                                                     cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGAg                                 >  2:2369482/1‑140 (MQ=255)

                                                                                                       caccgaccgGCGCCACCGGCGCCACGGGCGACCCCGGACCAGCCGGAGCCACACGAGCCACCGGCGAAGCCGGACCCACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCt                               <  2:43903/139‑1 (MQ=17)

                                                                                                        aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAGCAGCCGGAGCGACAGGAGCTa                              >  1:574027/1‑140 (MQ=255)

                                                                                                           aaCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCg                           >  1:3127013/1‑140 (MQ=255)

                                                                                                                      cAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGCCGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                <  1:692814/140‑1 (MQ=255)

                                                                                                                       aGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc               >  1:2558807/1‑140 (MQ=255)

                                                                                                                       aGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc               >  1:3452022/1‑140 (MQ=255)

                                                                                                                            cTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc          >  1:2974676/1‑140 (MQ=255)

                                                                                                                            cTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc          >  1:2201594/1‑140 (MQ=255)

                                                                                                                              actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg        >  2:1431342/4‑140 (MQ=255)

                                                                                                                              actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg        >  2:199774/4‑140 (MQ=255)

                                                                                                                              actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGCACCAGCCGGAGCGACAGGAGCTACCGGGTAAGCCGGACCAACCgg        >  2:2930219/4‑140 (MQ=17)

                                                                                                                              actggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGa                                                                               <  1:2229252/66‑1 (MQ=0)

                                                                                                                              actggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGa                                                                               >  2:3589678/4‑69 (MQ=25)

                                                                                                                              aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg        >  1:2428969/1‑140 (MQ=255)

                                                                                                                              aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg        >  2:2030969/1‑140 (MQ=255)

                                                                                                                                cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACGGGGGACGTCGGCCCAGCCGGATCGACAGTAGCTACCGGGGAAGCCGGACCAACCGGac      >  2:1402981/1‑139 (MQ=18)

                                                                                                                                cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg      >  2:3179297/1‑140 (MQ=255)

                                                                                                                                    gcaGCCGGACCCGCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc  <  2:1657854/138‑1 (MQ=255)

                                                                                                                                    gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                     <  2:3405270/121‑1 (MQ=255)

                                                                                                                                    gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc  >  2:2107083/1‑140 (MQ=255)

                                                                                                                                    gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc  <  1:1989038/140‑1 (MQ=255)

                                                                                                                                    gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc  <  1:122941/140‑1 (MQ=255)

                                                                                                                                    gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc  <  1:2369482/140‑1 (MQ=255)

                                                                                                                                    gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc  >  2:782350/1‑140 (MQ=255)

                                                                                                                                    gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGCACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGGGACGTCGGACCAGCCGTAGCGAAAGGAGCTACCGGGGAAGCCGGACCAACAGGAGCGAc  >  2:2032446/1‑140 (MQ=18)

                                                                                                                                      aGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTgg                                                              >  2:1881064/1‑78 (MQ=255)

                                                                                                                                      aGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTgg                                                              <  1:1881064/78‑1 (MQ=255)

ACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAC  >  NZ_CP034943/607566‑607837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`