Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 634,052 T→G 17.4% E573D (GAA→GAC EO946_RS03330 ← ABC‑F family ATP‑binding cassette domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943634,0520TG17.4% 334.1 / 25.5 178E573D (GAA→GACEO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (57/83);  new base G (35/2);  total (92/86)
Fisher's exact test for biased strand distribution p-value = 1.01e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 186 total aligned reads displayed.

GTCTGCATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAAT  >  NZ_CP034943/633914‑634191
                                                                                                                                          |                                                                                                                                            
ttCTGCATGGATGGCTTGTACCTTTTCATGGTGTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGtt                                                                                                                                             <  2:2132672/139‑1 (MQ=255)
     cttggatggCTTGTACCTTTTCTTGGTCTTGATAGATTTCCGGATCACATACCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAAGAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGttctttc                                                                                                                                        <  2:2163127/138‑1 (MQ=255)
     cATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGttggttg                                                                                                                                        >  1:3867412/1‑135 (MQ=255)
     cATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGCGgtatttt                                                                                                                                        >  1:1018373/1‑139 (MQ=255)
          atggCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTGGTTTGttttt                                                                                                                                   >  1:2928706/1‑140 (MQ=255)
          atggCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCttttt                                                                                                                                   >  2:1305431/1‑140 (MQ=255)
           tggCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGGGTTCTTTAttttta                                                                                                                                  >  2:1618865/1‑139 (MQ=255)
           tggCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTCTTTCtttttg                                                                                                                                  >  2:2460721/1‑139 (MQ=255)
           tggCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTGGTTTGtttttg                                                                                                                                  >  1:1445498/1‑139 (MQ=255)
           tggCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTCttttta                                                                                                                                  >  1:1839823/1‑139 (MQ=255)
           tggCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTc                                                                                                                                  <  1:986245/140‑1 (MQ=255)
           tggCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTCttttta                                                                                                                                  >  2:771555/1‑139 (MQ=255)
           tggCTTGTACATTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTCATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATGCGACTGAGTCGTTGGCGTTGTTTCtttttg                                                                                                                                  >  2:1058156/1‑139 (MQ=255)
             gCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCa                                                                                                                                <  2:2834645/140‑1 (MQ=255)
             gCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCa                                                                                                                                <  2:1001803/140‑1 (MQ=255)
             gCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTGTGTTTCCa                                                                                                                                >  2:2141663/1‑140 (MQ=255)
              cTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCAt                                                                                                                               <  2:3538909/140‑1 (MQ=255)
              cTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCAt                                                                                                                               <  2:1338529/140‑1 (MQ=255)
              cTTGTACCTTTTCATGGTCTTGATAGATGTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCAt                                                                                                                               <  2:3299459/140‑1 (MQ=255)
               ttGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGCGTTCTTTGtgtttgcctt                                                                                                                              >  1:227089/1‑135 (MQ=255)
                      ttttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATTGTTATGACTAATGTTTTCTTCTATGTTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTttc                                                                                                                       <  2:2520331/140‑1 (MQ=255)
                       tttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTTATTTTTCAATTAtttttat                                                                                                                      >  2:13862/1‑138 (MQ=255)
                       tttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAAATTCTTCAATCCGACGGAGTCGTTGGGGTTCTTTATTTTTACATTAtttttat                                                                                                                      >  2:3159723/1‑138 (MQ=255)
                        ttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGCGTTGGTTCTTTTTTCCTTAtttttttt                                                                                                                     >  2:3479856/1‑140 (MQ=255)
                        ttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTTATTTTTACATTCTTTttatt                                                                                                                     >  2:652950/1‑140 (MQ=255)
                        ttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGTTTGTTTTTCCAATATTTttatt                                                                                                                     >  1:1864696/1‑140 (MQ=255)
                        ttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTCTTTTTGCATTATTTttatt                                                                                                                     >  2:1666198/1‑140 (MQ=255)
                        ttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTttctt                                                                                                                     <  1:770378/140‑1 (MQ=255)
                        ttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTttctt                                                                                                                     <  1:524762/140‑1 (MQ=255)
                        ttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAAGGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTATTTTTGGATTGTTTttgtt                                                                                                                     >  1:3601361/1‑140 (MQ=255)
                         tCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTttcttc                                                                                                                    <  2:1485610/140‑1 (MQ=255)
                          cATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGTCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTttcttct                                                                                                                   <  2:1005879/140‑1 (MQ=255)
                          cATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTttcttct                                                                                                                   <  2:3646499/140‑1 (MQ=255)
                          cATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTttcttct                                                                                                                   <  2:2289826/140‑1 (MQ=255)
                            tGGTCTTGATAGATGTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTTGTTTTTGAATTATTTttattgtta                                                                                                                 >  1:979561/1‑139 (MQ=255)
                               tCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGGGTTCTTTCTTTTTGCATTATTTTTCTTATTAata                                                                                                              >  2:1811869/1‑140 (MQ=255)
                               tCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGTGCGTTGTTTATTTTTCCCTTATTTTTCTTGTTCata                                                                                                              >  2:804580/1‑140 (MQ=255)
                                 ttGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATg                                                                                                            <  2:1071285/140‑1 (MQ=255)
                                 ttGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGCTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCGTTTTACAATATATTTCTTATTCAAATg                                                                                                            >  2:859738/1‑140 (MQ=255)
                                   gATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGGGTTGTTTCTTTTTGCAGTGTTTTTCTTCTTCATATTaa                                                                                                         >  2:443414/1‑140 (MQ=255)
                                   gATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGCGTTGTTTATTTTTCCATTATTTTTATTATTAatattta                                                                                                          >  2:2688193/1‑137 (MQ=255)
                                   gATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGaa                                                                                                         <  1:1955298/140‑1 (MQ=255)
                                        aTTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGttt                                                                                                    <  2:3157999/140‑1 (MQ=255)
                                        aTTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGttt                                                                                                    <  2:2508852/140‑1 (MQ=255)
                                         tttCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTCTTTTTCCATTCTTTTTGTTCTTAATATGAACGtttt                                                                                                   >  2:3432218/1‑140 (MQ=255)
                                         tttCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGGTCGTTTTTTTTGCATTATTTTTCTTCTTAATATGAAAGtttt                                                                                                   >  1:2197046/1‑140 (MQ=255)
                                           tCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTGTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGa                                                                                                 >  2:3670403/1‑140 (MQ=255)
                                           tCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCAATCTTTTTATTCTTAATATGAAAGGTTTGa                                                                                                 >  2:2697462/1‑140 (MQ=255)
                                           tCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTATTTTTGCATTATTTTTCTTCTTCATATGAAAGTTTTGa                                                                                                 >  2:2901013/1‑140 (MQ=255)
                                           tCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCGTTCTTTTTCCCTTCTTTTTAGTATTCATATGAACGTTTTGa                                                                                                 >  2:1138070/1‑140 (MQ=255)
                                             cGGATCACATAGCAATTCATCGTTATGACTACTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                               <  2:2761572/140‑1 (MQ=255)
                                             cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                               <  1:459808/140‑1 (MQ=255)
                                             cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                               <  1:1305431/140‑1 (MQ=255)
                                             cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTATTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                               <  1:3380402/140‑1 (MQ=255)
                                             cGGATCACATAGCAAGTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCACTCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                               <  2:2914904/140‑1 (MQ=255)
                                             cGGAACACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGATCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                               <  1:1835733/140‑1 (MQ=255)
                                                       aGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAAc                                                                                     <  2:3143633/140‑1 (MQ=255)
                                                       aGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAAc                                                                                     <  2:2347596/140‑1 (MQ=255)
                                                       aGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAAc                                                                                     <  2:1825115/140‑1 (MQ=255)
                                                       aGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAAc                                                                                     <  2:1543577/140‑1 (MQ=255)
                                                            ttCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGGTTGGTTTTGCATTCTTTTTCTTGTTCATATTAAAGTTTTTTGTCATGGTTaaaccaac                                                                                >  1:3755122/1‑134 (MQ=255)
                                                             tCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGGTTCTTTTTGCCTTGTTTTTCTTGTTCATAAGAACGTTTTTAGTCAGGCTTAAACGAAGc                                                                               >  2:935641/1‑140 (MQ=255)
                                                             tCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGGGTTTTTGTTTTCATTAGTGTTGTTGTTAATATGAACGTTTTTAATGAGGCTTAACAGAAGc                                                                               >  1:2642008/1‑140 (MQ=255)
                                                              cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTTGTTTTTACCGTATTTTTATTATTAAAATTAAAGTTTTTAGTGAGGGTTAAACCAAGCa                                                                              >  1:2623296/1‑140 (MQ=255)
                                                              cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGTTTGTTTTTGCATTCTTTTTGGTCTTCATATTAACGTTTTTTTTCATGCTTAAACAAAGCa                                                                              >  1:2593562/1‑140 (MQ=255)
                                                              cATCGTTATGACTAATGTTTTCTTCAAGGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGGGGTTTGTTTTTGCCTTCTTTTTGTTCTTGATTTGTAGCGTTTTATTGAGGCTTGAACGAAGCa                                                                              >  1:168974/1‑140 (MQ=255)
                                                                 cGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGCGTGGGTTCTTTTTGCATTCTTTTTGTTCTTCATATGTACGTTTTTAGTAAGGCTTAAACGAATCAGGc                                                                           >  2:2105406/1‑140 (MQ=255)
                                                                 cGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTGCTTTATTTTTACATTATTTTTCTTATTCATATGAACGTTTTGAGTCAGGCTTAACCGCAGCACGc                                                                           >  2:1167825/1‑140 (MQ=255)
                                                                 cGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGc                                                                           >  2:900058/1‑140 (MQ=255)
                                                                       gACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGttttt                                                                     <  2:1176826/140‑1 (MQ=255)
                                                                       gACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGttttt                                                                     <  2:2724643/140‑1 (MQ=255)
                                                                       gACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGttttt                                                                     <  2:3759415/140‑1 (MQ=255)
                                                                           aaTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTAGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCgg                                                                 <  2:2227087/140‑1 (MQ=255)
                                                                            ctGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTATTCCATTCATTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGa                                                                <  1:3032082/139‑1 (MQ=255)
                                                                             tGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTGTTTTTGCATTATTTTTATTATTCATATTAACGTTTTGTGTCAGGCTTAAACGAAACAGGCGTTTTTTGGGAt                                                               >  2:595427/1‑140 (MQ=255)
                                                                                 ttcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGTTTGTTTTTTGAATATTTTTATTGTTAATATTAAAGTTTTTTGTAAGGGGTAAAACAAGCAGGCGTTTTTTgggtgtgct                                                           >  1:1658682/1‑134 (MQ=255)
                                                                                  tcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGGTTGTTTTTGCCTTGTTTTTATTTTTGATATTTACGTTTTTTTTGAGGGTTAACCGAATCAGGCGTTTTTTGGGtttgggg                                                          >  1:3564724/1‑136 (MQ=255)
                                                                                  tcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTATTTTTACATTCATTTTCTTCATCATAAGAACGTTTTTATTCAGGCTTAACCGAAGCATGCGTTTTTTAGGAttacaa                                                          >  2:3780933/1‑136 (MQ=255)
                                                                                  tcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGGTGGTTGTTTTTGCAATGTTTTTGTTGTTGATATGAAAGGTTTTAGTGATGCTTTAACCAAGCACGCGTTTTTTTGGAttgcga                                                          >  1:1033725/1‑136 (MQ=255)
                                                                                    ttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTGTTTTTGCATTGTTTTTCTTCTTCAAATGAAAGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGGTTACACtt                                                        >  2:1906810/1‑140 (MQ=255)
                                                                                     tcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTg                                                       <  1:2697462/140‑1 (MQ=255)
                                                                                     tcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTg                                                       <  2:2313099/140‑1 (MQ=255)
                                                                                                   aGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTtct                                         <  2:2623296/140‑1 (MQ=255)
                                                                                                   aGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTtct                                         <  2:979561/140‑1 (MQ=255)
                                                                                                     tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTCTTTGTTTTTCCATTCTTTTTGTTCTTAATATGAAAGTTTTTTGTCAGGCTTAAACCAAGCAGGCGTTTTTTGGGATTCCTCTTGGTGTTTAAttttatac                                       >  2:2482344/1‑136 (MQ=255)
                                                                                                        cTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAAt                                    <  1:900058/140‑1 (MQ=255)
                                                                                                             aTTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAg                               <  2:662471/140‑1 (MQ=255)
                                                                                                                  ttcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                          <  2:2452355/140‑1 (MQ=255)
                                                                                                                  ttcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                          <  1:595427/140‑1 (MQ=255)
                                                                                                                  ttcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                          <  1:209456/140‑1 (MQ=255)
                                                                                                                            cGGAGTCGTTTGCGTTGTTTGTTTTTGCATTGTTTTTCTTGTTCATATGAACGTTTTGAGTCAGGGTTAAACGAAGCAGGCGTTTTTTAGGATTCCACTTGCTGATTCATTTTCTCCAAATCAAACTGGTGGtttttttt                >  2:3842876/1‑140 (MQ=255)
                                                                                                                            cGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt                <  1:1242207/140‑1 (MQ=255)
                                                                                                                            cGGAGTCGTTGGCGGTCTTTGTTTTTACATTATTTTTATTCTTAATATTAACGTTTTGATTCAGGCTTAACCGAAGCAGGCGTTTTTTGGGTTTCCGATTTCTGATTGATTTTCTCCAATTCAAGCTGGTCTGttttttt                >  2:1934932/1‑140 (MQ=255)
                                                                                                                            cGGAGTCGTTGGCGGTCTTTCTTTTTCCAGTCTTTTTCTTCTTAATATGTACCTTTTGAGTCAGGCTTAAACGAAGCAGGCGTTTTTTCGGATTACTCGTGCGGATTCATTTTGTCCAATTCAAACTGGTATGttttttt                >  2:3020060/1‑140 (MQ=255)
                                                                                                                              gAGTCGGTGGCGGTCTTACAGATTCCACTCTTTGTCGTCGTCATAGGACCGTTGTGAGTCAGGCTTACCCGCAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATGCATTTTCTCCAATTCAAGCTGCTCTGTTTTGTtct              <  1:2474123/140‑1 (MQ=255)
                                                                                                                              gAGTCGGTGGCGGTCGTTCTTTCGCCAGTCTTTGTCTTCCTCATATCCCCGTTGTGAGTCAGGCTTAACCGCAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCGGTTTTTTtct              <  1:3205276/140‑1 (MQ=255)
                                                                                                                                 tCGTTGGGGGTGTTTGTTTTTGCATTCTTTTTGTTGTTGATATGAACGTTTTGAGTCACGGTTAAACGAAGCCGGCGTTTTTTGGGTTTACTCGTTGGGATTCATTTTCTGCAAATAAAGCTGCGGGGtttttttatgcg           >  2:1516847/1‑135 (MQ=255)
                                                                                                                                      ggCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTataa      <  1:2688193/140‑1 (MQ=255)
                                                                                                                                          ttctttctTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATaataat  <  1:3176539/140‑1 (MQ=255)
                                                                                                                                          ttctttctTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATaataat  <  1:2760709/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
GTCTGCATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAAT  >  NZ_CP034943/633914‑634191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: